Halothiobacillus neapolitanus (strain ATCC 23641 / c2) (Thiobacillus neapolitanus)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Halothiobacillaceae; Halothiobacillus; Halothiobacillus neapolitanus

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2354 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D0L086|D0L086_HALNC Adenylate kinase OS=Halothiobacillus neapolitanus (strain ATCC 23641 / c2) OX=555778 GN=adk PE=3 SV=1
MM1 pKa = 7.33TVPIDD6 pKa = 3.38RR7 pKa = 11.84RR8 pKa = 11.84YY9 pKa = 10.58GPTHH13 pKa = 5.66EE14 pKa = 4.63WYY16 pKa = 10.83LLEE19 pKa = 6.43DD20 pKa = 4.45DD21 pKa = 5.25LLVLGVTEE29 pKa = 4.24QGQALLGDD37 pKa = 3.86VVFAQLPDD45 pKa = 3.62VGSHH49 pKa = 4.49VQRR52 pKa = 11.84GQACATLEE60 pKa = 4.27SVKK63 pKa = 10.55AASDD67 pKa = 3.74VICPVDD73 pKa = 3.58GVVIAVNSALSDD85 pKa = 3.74SPEE88 pKa = 5.07LINDD92 pKa = 3.74DD93 pKa = 4.35PFDD96 pKa = 4.07TGWILHH102 pKa = 5.86IHH104 pKa = 6.71PSGSPDD110 pKa = 2.45EE111 pKa = 4.31WMSPEE116 pKa = 4.35EE117 pKa = 3.93YY118 pKa = 10.28LAQAEE123 pKa = 4.26

Molecular weight: 13.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D0KXN8|D0KXN8_HALNC 50S ribosomal protein L17 OS=Halothiobacillus neapolitanus (strain ATCC 23641 / c2) OX=555778 GN=rplQ PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.37RR3 pKa = 11.84TYY5 pKa = 9.98QPSIIHH11 pKa = 6.99RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.7GFRR19 pKa = 11.84ARR21 pKa = 11.84MATRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2353

1

2354

761027

31

2507

323.3

35.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.688 ± 0.062

0.823 ± 0.016

5.636 ± 0.036

5.431 ± 0.053

3.788 ± 0.033

7.553 ± 0.049

2.415 ± 0.02

5.747 ± 0.04

3.74 ± 0.039

10.553 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.545 ± 0.025

3.344 ± 0.035

5.002 ± 0.036

4.385 ± 0.042

6.173 ± 0.051

5.881 ± 0.032

5.471 ± 0.04

7.038 ± 0.04

1.39 ± 0.025

2.397 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski