Amino acid dipepetide frequency for Methylibium sp. NZG

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.356AlaAla: 20.356 ± 0.224
1.357AlaCys: 1.357 ± 0.038
7.365AlaAsp: 7.365 ± 0.076
6.798AlaGlu: 6.798 ± 0.088
4.263AlaPhe: 4.263 ± 0.066
11.601AlaGly: 11.601 ± 0.126
2.917AlaHis: 2.917 ± 0.059
4.728AlaIle: 4.728 ± 0.069
3.956AlaLys: 3.956 ± 0.072
15.784AlaLeu: 15.784 ± 0.163
3.49AlaMet: 3.49 ± 0.054
2.865AlaAsn: 2.865 ± 0.06
6.904AlaPro: 6.904 ± 0.102
5.937AlaGln: 5.937 ± 0.074
9.345AlaArg: 9.345 ± 0.118
7.231AlaSer: 7.231 ± 0.1
7.051AlaThr: 7.051 ± 0.093
9.874AlaVal: 9.874 ± 0.106
2.134AlaTrp: 2.134 ± 0.049
2.327AlaTyr: 2.327 ± 0.047
0.0AlaXaa: 0.0 ± 0.0
Cys
1.179CysAla: 1.179 ± 0.038
0.095CysCys: 0.095 ± 0.009
0.578CysAsp: 0.578 ± 0.02
0.506CysGlu: 0.506 ± 0.022
0.308CysPhe: 0.308 ± 0.015
0.944CysGly: 0.944 ± 0.029
0.284CysHis: 0.284 ± 0.015
0.403CysIle: 0.403 ± 0.019
0.249CysLys: 0.249 ± 0.016
0.763CysLeu: 0.763 ± 0.024
0.191CysMet: 0.191 ± 0.012
0.236CysAsn: 0.236 ± 0.012
0.471CysPro: 0.471 ± 0.019
0.22CysGln: 0.22 ± 0.013
0.55CysArg: 0.55 ± 0.023
0.518CysSer: 0.518 ± 0.022
0.516CysThr: 0.516 ± 0.025
0.755CysVal: 0.755 ± 0.024
0.122CysTrp: 0.122 ± 0.01
0.191CysTyr: 0.191 ± 0.012
0.0CysXaa: 0.0 ± 0.0
Asp
7.923AspAla: 7.923 ± 0.096
0.409AspCys: 0.409 ± 0.019
2.795AspAsp: 2.795 ± 0.053
3.172AspGlu: 3.172 ± 0.055
1.89AspPhe: 1.89 ± 0.049
4.699AspGly: 4.699 ± 0.093
1.129AspHis: 1.129 ± 0.03
2.131AspIle: 2.131 ± 0.041
1.712AspLys: 1.712 ± 0.039
5.025AspLeu: 5.025 ± 0.079
1.117AspMet: 1.117 ± 0.029
1.182AspAsn: 1.182 ± 0.031
2.954AspPro: 2.954 ± 0.039
1.4AspGln: 1.4 ± 0.034
3.433AspArg: 3.433 ± 0.051
2.28AspSer: 2.28 ± 0.045
2.643AspThr: 2.643 ± 0.067
4.289AspVal: 4.289 ± 0.061
0.9AspTrp: 0.9 ± 0.028
1.218AspTyr: 1.218 ± 0.035
0.0AspXaa: 0.0 ± 0.0
Glu
6.598GluAla: 6.598 ± 0.09
0.348GluCys: 0.348 ± 0.016
1.854GluAsp: 1.854 ± 0.044
2.025GluGlu: 2.025 ± 0.052
1.796GluPhe: 1.796 ± 0.039
3.431GluGly: 3.431 ± 0.061
1.346GluHis: 1.346 ± 0.03
2.21GluIle: 2.21 ± 0.04
1.547GluLys: 1.547 ± 0.044
5.904GluLeu: 5.904 ± 0.082
1.203GluMet: 1.203 ± 0.031
1.028GluAsn: 1.028 ± 0.032
2.85GluPro: 2.85 ± 0.054
2.395GluGln: 2.395 ± 0.047
4.794GluArg: 4.794 ± 0.074
2.283GluSer: 2.283 ± 0.047
2.224GluThr: 2.224 ± 0.038
4.122GluVal: 4.122 ± 0.066
0.679GluTrp: 0.679 ± 0.023
0.93GluTyr: 0.93 ± 0.03
0.0GluXaa: 0.0 ± 0.0
Phe
4.346PheAla: 4.346 ± 0.066
0.397PheCys: 0.397 ± 0.014
2.647PheAsp: 2.647 ± 0.048
2.182PheGlu: 2.182 ± 0.04
1.309PhePhe: 1.309 ± 0.036
3.541PheGly: 3.541 ± 0.051
0.72PheHis: 0.72 ± 0.024
1.607PheIle: 1.607 ± 0.04
1.261PheLys: 1.261 ± 0.032
2.689PheLeu: 2.689 ± 0.05
0.802PheMet: 0.802 ± 0.026
1.178PheAsn: 1.178 ± 0.03
1.46PhePro: 1.46 ± 0.03
0.956PheGln: 0.956 ± 0.031
1.828PheArg: 1.828 ± 0.038
2.041PheSer: 2.041 ± 0.04
1.985PheThr: 1.985 ± 0.045
2.876PheVal: 2.876 ± 0.052
0.509PheTrp: 0.509 ± 0.021
0.761PheTyr: 0.761 ± 0.025
0.0PheXaa: 0.0 ± 0.0
Gly
10.375GlyAla: 10.375 ± 0.113
0.933GlyCys: 0.933 ± 0.029
4.14GlyAsp: 4.14 ± 0.074
4.257GlyGlu: 4.257 ± 0.066
3.29GlyPhe: 3.29 ± 0.055
7.636GlyGly: 7.636 ± 0.136
1.996GlyHis: 1.996 ± 0.039
3.563GlyIle: 3.563 ± 0.063
3.235GlyLys: 3.235 ± 0.05
9.03GlyLeu: 9.03 ± 0.087
2.282GlyMet: 2.282 ± 0.05
2.216GlyAsn: 2.216 ± 0.095
3.366GlyPro: 3.366 ± 0.054
3.192GlyGln: 3.192 ± 0.066
5.658GlyArg: 5.658 ± 0.08
5.038GlySer: 5.038 ± 0.113
4.709GlyThr: 4.709 ± 0.108
7.038GlyVal: 7.038 ± 0.073
1.55GlyTrp: 1.55 ± 0.039
2.049GlyTyr: 2.049 ± 0.04
0.0GlyXaa: 0.0 ± 0.0
His
3.116HisAla: 3.116 ± 0.056
0.271HisCys: 0.271 ± 0.016
1.219HisAsp: 1.219 ± 0.033
1.088HisGlu: 1.088 ± 0.033
0.937HisPhe: 0.937 ± 0.027
2.282HisGly: 2.282 ± 0.045
0.678HisHis: 0.678 ± 0.023
0.862HisIle: 0.862 ± 0.026
0.564HisLys: 0.564 ± 0.026
2.214HisLeu: 2.214 ± 0.047
0.426HisMet: 0.426 ± 0.02
0.5HisAsn: 0.5 ± 0.02
1.462HisPro: 1.462 ± 0.037
0.727HisGln: 0.727 ± 0.025
1.573HisArg: 1.573 ± 0.036
1.006HisSer: 1.006 ± 0.031
1.116HisThr: 1.116 ± 0.03
1.686HisVal: 1.686 ± 0.038
0.399HisTrp: 0.399 ± 0.018
0.548HisTyr: 0.548 ± 0.022
0.0HisXaa: 0.0 ± 0.0
Ile
5.724IleAla: 5.724 ± 0.072
0.35IleCys: 0.35 ± 0.016
3.119IleAsp: 3.119 ± 0.049
2.966IleGlu: 2.966 ± 0.051
1.148IlePhe: 1.148 ± 0.034
4.048IleGly: 4.048 ± 0.065
0.791IleHis: 0.791 ± 0.023
1.265IleIle: 1.265 ± 0.037
1.352IleLys: 1.352 ± 0.04
2.648IleLeu: 2.648 ± 0.05
0.632IleMet: 0.632 ± 0.025
1.276IleAsn: 1.276 ± 0.033
1.758IlePro: 1.758 ± 0.04
1.056IleGln: 1.056 ± 0.028
2.261IleArg: 2.261 ± 0.043
2.049IleSer: 2.049 ± 0.045
2.337IleThr: 2.337 ± 0.043
3.599IleVal: 3.599 ± 0.062
0.359IleTrp: 0.359 ± 0.017
0.833IleTyr: 0.833 ± 0.024
0.0IleXaa: 0.0 ± 0.0
Lys
4.266LysAla: 4.266 ± 0.07
0.175LysCys: 0.175 ± 0.013
1.579LysAsp: 1.579 ± 0.043
1.347LysGlu: 1.347 ± 0.033
0.935LysPhe: 0.935 ± 0.029
2.444LysGly: 2.444 ± 0.05
0.69LysHis: 0.69 ± 0.023
1.225LysIle: 1.225 ± 0.035
1.302LysLys: 1.302 ± 0.051
3.599LysLeu: 3.599 ± 0.056
0.785LysMet: 0.785 ± 0.023
0.845LysAsn: 0.845 ± 0.029
2.069LysPro: 2.069 ± 0.04
1.094LysGln: 1.094 ± 0.03
2.355LysArg: 2.355 ± 0.046
1.711LysSer: 1.711 ± 0.041
1.826LysThr: 1.826 ± 0.041
2.675LysVal: 2.675 ± 0.057
0.334LysTrp: 0.334 ± 0.016
0.613LysTyr: 0.613 ± 0.022
0.0LysXaa: 0.0 ± 0.0
Leu
15.359LeuAla: 15.359 ± 0.148
0.982LeuCys: 0.982 ± 0.029
5.73LeuAsp: 5.73 ± 0.079
4.559LeuGlu: 4.559 ± 0.071
3.361LeuPhe: 3.361 ± 0.051
8.825LeuGly: 8.825 ± 0.087
2.36LeuHis: 2.36 ± 0.045
4.022LeuIle: 4.022 ± 0.076
3.77LeuLys: 3.77 ± 0.063
11.177LeuLeu: 11.177 ± 0.149
2.605LeuMet: 2.605 ± 0.051
2.741LeuAsn: 2.741 ± 0.05
6.212LeuPro: 6.212 ± 0.084
4.134LeuGln: 4.134 ± 0.061
7.89LeuArg: 7.89 ± 0.104
5.952LeuSer: 5.952 ± 0.073
5.428LeuThr: 5.428 ± 0.078
8.113LeuVal: 8.113 ± 0.079
1.392LeuTrp: 1.392 ± 0.039
1.913LeuTyr: 1.913 ± 0.041
0.0LeuXaa: 0.0 ± 0.0
Met
3.04MetAla: 3.04 ± 0.05
0.2MetCys: 0.2 ± 0.013
1.066MetAsp: 1.066 ± 0.028
0.93MetGlu: 0.93 ± 0.027
0.756MetPhe: 0.756 ± 0.023
1.816MetGly: 1.816 ± 0.045
0.511MetHis: 0.511 ± 0.022
0.928MetIle: 0.928 ± 0.031
1.078MetLys: 1.078 ± 0.031
2.737MetLeu: 2.737 ± 0.05
0.56MetMet: 0.56 ± 0.022
0.862MetAsn: 0.862 ± 0.027
1.482MetPro: 1.482 ± 0.037
1.039MetGln: 1.039 ± 0.027
1.74MetArg: 1.74 ± 0.039
1.49MetSer: 1.49 ± 0.038
1.484MetThr: 1.484 ± 0.039
1.732MetVal: 1.732 ± 0.043
0.21MetTrp: 0.21 ± 0.012
0.355MetTyr: 0.355 ± 0.017
0.0MetXaa: 0.0 ± 0.0
Asn
3.364AsnAla: 3.364 ± 0.067
0.249AsnCys: 0.249 ± 0.015
1.325AsnAsp: 1.325 ± 0.067
1.145AsnGlu: 1.145 ± 0.027
0.924AsnPhe: 0.924 ± 0.026
2.183AsnGly: 2.183 ± 0.068
0.528AsnHis: 0.528 ± 0.02
1.104AsnIle: 1.104 ± 0.033
0.759AsnLys: 0.759 ± 0.028
2.576AsnLeu: 2.576 ± 0.059
0.495AsnMet: 0.495 ± 0.018
0.684AsnAsn: 0.684 ± 0.04
1.766AsnPro: 1.766 ± 0.039
0.81AsnGln: 0.81 ± 0.026
1.579AsnArg: 1.579 ± 0.04
1.158AsnSer: 1.158 ± 0.035
1.438AsnThr: 1.438 ± 0.043
2.022AsnVal: 2.022 ± 0.041
0.364AsnTrp: 0.364 ± 0.017
0.61AsnTyr: 0.61 ± 0.035
0.0AsnXaa: 0.0 ± 0.0
Pro
7.977ProAla: 7.977 ± 0.113
0.362ProCys: 0.362 ± 0.017
2.858ProAsp: 2.858 ± 0.048
2.996ProGlu: 2.996 ± 0.046
1.836ProPhe: 1.836 ± 0.04
4.784ProGly: 4.784 ± 0.069
1.186ProHis: 1.186 ± 0.03
1.855ProIle: 1.855 ± 0.041
1.638ProLys: 1.638 ± 0.038
5.303ProLeu: 5.303 ± 0.082
1.33ProMet: 1.33 ± 0.034
1.34ProAsn: 1.34 ± 0.038
3.139ProPro: 3.139 ± 0.07
2.204ProGln: 2.204 ± 0.041
3.171ProArg: 3.171 ± 0.053
2.831ProSer: 2.831 ± 0.055
3.053ProThr: 3.053 ± 0.054
4.345ProVal: 4.345 ± 0.061
0.805ProTrp: 0.805 ± 0.027
1.055ProTyr: 1.055 ± 0.032
0.0ProXaa: 0.0 ± 0.0
Gln
5.679GlnAla: 5.679 ± 0.077
0.266GlnCys: 0.266 ± 0.015
1.399GlnAsp: 1.399 ± 0.037
1.248GlnGlu: 1.248 ± 0.037
1.244GlnPhe: 1.244 ± 0.03
2.943GlnGly: 2.943 ± 0.044
0.908GlnHis: 0.908 ± 0.029
1.624GlnIle: 1.624 ± 0.04
0.957GlnLys: 0.957 ± 0.029
3.852GlnLeu: 3.852 ± 0.055
0.985GlnMet: 0.985 ± 0.029
0.682GlnAsn: 0.682 ± 0.026
2.25GlnPro: 2.25 ± 0.046
1.664GlnGln: 1.664 ± 0.045
3.726GlnArg: 3.726 ± 0.059
1.869GlnSer: 1.869 ± 0.04
1.868GlnThr: 1.868 ± 0.04
3.041GlnVal: 3.041 ± 0.052
0.625GlnTrp: 0.625 ± 0.023
0.642GlnTyr: 0.642 ± 0.026
0.0GlnXaa: 0.0 ± 0.0
Arg
8.632ArgAla: 8.632 ± 0.114
0.657ArgCys: 0.657 ± 0.024
3.777ArgAsp: 3.777 ± 0.06
4.006ArgGlu: 4.006 ± 0.057
2.869ArgPhe: 2.869 ± 0.045
5.092ArgGly: 5.092 ± 0.069
1.861ArgHis: 1.861 ± 0.036
3.196ArgIle: 3.196 ± 0.046
1.977ArgLys: 1.977 ± 0.042
7.953ArgLeu: 7.953 ± 0.112
1.893ArgMet: 1.893 ± 0.039
1.688ArgAsn: 1.688 ± 0.033
3.29ArgPro: 3.29 ± 0.055
2.61ArgGln: 2.61 ± 0.047
5.079ArgArg: 5.079 ± 0.074
3.748ArgSer: 3.748 ± 0.057
3.264ArgThr: 3.264 ± 0.049
5.46ArgVal: 5.46 ± 0.069
1.299ArgTrp: 1.299 ± 0.038
1.65ArgTyr: 1.65 ± 0.04
0.0ArgXaa: 0.0 ± 0.0
Ser
7.217SerAla: 7.217 ± 0.094
0.414SerCys: 0.414 ± 0.02
2.516SerAsp: 2.516 ± 0.046
2.304SerGlu: 2.304 ± 0.045
2.115SerPhe: 2.115 ± 0.045
5.321SerGly: 5.321 ± 0.12
1.122SerHis: 1.122 ± 0.028
2.196SerIle: 2.196 ± 0.044
1.489SerLys: 1.489 ± 0.037
5.668SerLeu: 5.668 ± 0.07
1.334SerMet: 1.334 ± 0.03
1.342SerAsn: 1.342 ± 0.047
2.924SerPro: 2.924 ± 0.052
1.703SerGln: 1.703 ± 0.041
3.403SerArg: 3.403 ± 0.054
2.897SerSer: 2.897 ± 0.061
2.999SerThr: 2.999 ± 0.062
4.295SerVal: 4.295 ± 0.076
0.679SerTrp: 0.679 ± 0.026
1.063SerTyr: 1.063 ± 0.033
0.0SerXaa: 0.0 ± 0.0
Thr
6.525ThrAla: 6.525 ± 0.101
0.432ThrCys: 0.432 ± 0.023
2.627ThrAsp: 2.627 ± 0.05
2.395ThrGlu: 2.395 ± 0.048
1.764ThrPhe: 1.764 ± 0.04
4.883ThrGly: 4.883 ± 0.093
1.184ThrHis: 1.184 ± 0.031
2.013ThrIle: 2.013 ± 0.05
1.26ThrLys: 1.26 ± 0.037
6.573ThrLeu: 6.573 ± 0.09
1.153ThrMet: 1.153 ± 0.03
1.232ThrAsn: 1.232 ± 0.042
3.671ThrPro: 3.671 ± 0.062
1.925ThrGln: 1.925 ± 0.038
3.359ThrArg: 3.359 ± 0.051
2.78ThrSer: 2.78 ± 0.058
3.085ThrThr: 3.085 ± 0.089
4.558ThrVal: 4.558 ± 0.081
0.672ThrTrp: 0.672 ± 0.026
1.011ThrTyr: 1.011 ± 0.037
0.0ThrXaa: 0.0 ± 0.0
Val
10.701ValAla: 10.701 ± 0.104
0.789ValCys: 0.789 ± 0.024
3.992ValAsp: 3.992 ± 0.057
4.128ValGlu: 4.128 ± 0.065
2.875ValPhe: 2.875 ± 0.047
6.29ValGly: 6.29 ± 0.075
1.676ValHis: 1.676 ± 0.034
3.369ValIle: 3.369 ± 0.061
2.657ValLys: 2.657 ± 0.051
8.778ValLeu: 8.778 ± 0.102
1.974ValMet: 1.974 ± 0.041
2.227ValAsn: 2.227 ± 0.054
4.297ValPro: 4.297 ± 0.062
2.94ValGln: 2.94 ± 0.048
5.398ValArg: 5.398 ± 0.07
4.241ValSer: 4.241 ± 0.063
4.209ValThr: 4.209 ± 0.089
6.881ValVal: 6.881 ± 0.09
1.108ValTrp: 1.108 ± 0.027
1.473ValTyr: 1.473 ± 0.034
0.0ValXaa: 0.0 ± 0.0
Trp
1.54TrpAla: 1.54 ± 0.037
0.189TrpCys: 0.189 ± 0.013
0.623TrpAsp: 0.623 ± 0.024
0.519TrpGlu: 0.519 ± 0.019
0.602TrpPhe: 0.602 ± 0.022
0.978TrpGly: 0.978 ± 0.028
0.391TrpHis: 0.391 ± 0.017
0.565TrpIle: 0.565 ± 0.02
0.483TrpLys: 0.483 ± 0.023
2.118TrpLeu: 2.118 ± 0.044
0.374TrpMet: 0.374 ± 0.016
0.433TrpAsn: 0.433 ± 0.02
0.785TrpPro: 0.785 ± 0.024
0.734TrpGln: 0.734 ± 0.026
1.27TrpArg: 1.27 ± 0.038
0.78TrpSer: 0.78 ± 0.025
0.692TrpThr: 0.692 ± 0.027
1.1TrpVal: 1.1 ± 0.032
0.286TrpTrp: 0.286 ± 0.016
0.271TrpTyr: 0.271 ± 0.013
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.368TyrAla: 2.368 ± 0.045
0.204TyrCys: 0.204 ± 0.014
1.092TyrAsp: 1.092 ± 0.041
0.997TyrGlu: 0.997 ± 0.034
0.875TyrPhe: 0.875 ± 0.024
1.813TyrGly: 1.813 ± 0.039
0.399TyrHis: 0.399 ± 0.018
0.704TyrIle: 0.704 ± 0.024
0.625TyrLys: 0.625 ± 0.026
2.134TyrLeu: 2.134 ± 0.043
0.38TyrMet: 0.38 ± 0.015
0.527TyrAsn: 0.527 ± 0.024
0.956TyrPro: 0.956 ± 0.028
0.73TyrGln: 0.73 ± 0.022
1.589TyrArg: 1.589 ± 0.037
1.097TyrSer: 1.097 ± 0.035
1.172TyrThr: 1.172 ± 0.036
1.543TyrVal: 1.543 ± 0.032
0.31TyrTrp: 0.31 ± 0.015
0.478TyrTyr: 0.478 ± 0.023
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3979 proteins (1311359 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski