Amino acid dipepetide frequency for Pseudomonas phage UMP151

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.944AlaAla: 17.944 ± 3.009
1.222AlaCys: 1.222 ± 0.392
6.338AlaAsp: 6.338 ± 0.577
8.4AlaGlu: 8.4 ± 0.836
3.589AlaPhe: 3.589 ± 0.519
10.843AlaGly: 10.843 ± 1.019
1.604AlaHis: 1.604 ± 0.392
4.963AlaIle: 4.963 ± 0.435
4.887AlaLys: 4.887 ± 0.798
13.897AlaLeu: 13.897 ± 0.978
3.436AlaMet: 3.436 ± 0.541
2.673AlaAsn: 2.673 ± 0.509
5.269AlaPro: 5.269 ± 0.59
6.185AlaGln: 6.185 ± 0.64
8.018AlaArg: 8.018 ± 0.927
6.872AlaSer: 6.872 ± 0.844
5.574AlaThr: 5.574 ± 0.778
6.414AlaVal: 6.414 ± 0.726
3.207AlaTrp: 3.207 ± 0.449
2.749AlaTyr: 2.749 ± 0.479
0.0AlaXaa: 0.0 ± 0.0
Cys
0.687CysAla: 0.687 ± 0.22
0.229CysCys: 0.229 ± 0.117
0.535CysAsp: 0.535 ± 0.203
0.382CysGlu: 0.382 ± 0.176
0.229CysPhe: 0.229 ± 0.121
0.84CysGly: 0.84 ± 0.244
0.229CysHis: 0.229 ± 0.127
0.153CysIle: 0.153 ± 0.106
0.305CysLys: 0.305 ± 0.146
0.382CysLeu: 0.382 ± 0.144
0.076CysMet: 0.076 ± 0.094
0.382CysAsn: 0.382 ± 0.149
0.687CysPro: 0.687 ± 0.26
0.382CysGln: 0.382 ± 0.166
1.222CysArg: 1.222 ± 0.372
0.916CysSer: 0.916 ± 0.334
0.382CysThr: 0.382 ± 0.219
0.687CysVal: 0.687 ± 0.229
0.0CysTrp: 0.0 ± 0.0
0.382CysTyr: 0.382 ± 0.164
0.0CysXaa: 0.0 ± 0.0
Asp
6.261AspAla: 6.261 ± 0.648
0.382AspCys: 0.382 ± 0.172
3.283AspAsp: 3.283 ± 0.446
4.429AspGlu: 4.429 ± 0.55
2.214AspPhe: 2.214 ± 0.512
5.574AspGly: 5.574 ± 0.614
1.222AspHis: 1.222 ± 0.376
2.902AspIle: 2.902 ± 0.589
1.374AspLys: 1.374 ± 0.324
5.116AspLeu: 5.116 ± 0.483
0.916AspMet: 0.916 ± 0.211
1.527AspAsn: 1.527 ± 0.299
2.825AspPro: 2.825 ± 0.415
3.283AspGln: 3.283 ± 0.508
3.513AspArg: 3.513 ± 0.568
2.825AspSer: 2.825 ± 0.458
2.902AspThr: 2.902 ± 0.378
4.734AspVal: 4.734 ± 0.662
0.764AspTrp: 0.764 ± 0.205
1.451AspTyr: 1.451 ± 0.313
0.0AspXaa: 0.0 ± 0.0
Glu
7.56GluAla: 7.56 ± 1.016
0.764GluCys: 0.764 ± 0.22
3.283GluAsp: 3.283 ± 0.539
4.276GluGlu: 4.276 ± 0.619
1.833GluPhe: 1.833 ± 0.422
2.138GluGly: 2.138 ± 0.365
1.451GluHis: 1.451 ± 0.445
2.825GluIle: 2.825 ± 0.51
3.436GluLys: 3.436 ± 0.62
7.178GluLeu: 7.178 ± 0.812
1.451GluMet: 1.451 ± 0.38
1.985GluAsn: 1.985 ± 0.417
1.527GluPro: 1.527 ± 0.464
4.887GluGln: 4.887 ± 0.665
5.192GluArg: 5.192 ± 0.679
3.513GluSer: 3.513 ± 0.552
2.214GluThr: 2.214 ± 0.394
3.436GluVal: 3.436 ± 0.605
1.145GluTrp: 1.145 ± 0.281
1.833GluTyr: 1.833 ± 0.306
0.0GluXaa: 0.0 ± 0.0
Phe
2.749PheAla: 2.749 ± 0.411
0.305PheCys: 0.305 ± 0.164
2.825PheAsp: 2.825 ± 0.575
1.756PheGlu: 1.756 ± 0.322
0.993PhePhe: 0.993 ± 0.282
3.207PheGly: 3.207 ± 0.469
0.535PheHis: 0.535 ± 0.187
1.145PheIle: 1.145 ± 0.267
0.764PheLys: 0.764 ± 0.283
2.596PheLeu: 2.596 ± 0.34
0.764PheMet: 0.764 ± 0.227
1.374PheAsn: 1.374 ± 0.311
0.993PhePro: 0.993 ± 0.306
1.298PheGln: 1.298 ± 0.295
2.902PheArg: 2.902 ± 0.452
1.833PheSer: 1.833 ± 0.44
1.604PheThr: 1.604 ± 0.371
1.527PheVal: 1.527 ± 0.364
0.458PheTrp: 0.458 ± 0.211
0.687PheTyr: 0.687 ± 0.244
0.0PheXaa: 0.0 ± 0.0
Gly
8.018GlyAla: 8.018 ± 1.166
0.916GlyCys: 0.916 ± 0.255
4.276GlyAsp: 4.276 ± 0.541
3.589GlyGlu: 3.589 ± 0.413
2.367GlyPhe: 2.367 ± 0.336
5.422GlyGly: 5.422 ± 0.601
1.451GlyHis: 1.451 ± 0.37
3.207GlyIle: 3.207 ± 0.455
4.505GlyLys: 4.505 ± 0.602
7.865GlyLeu: 7.865 ± 0.882
2.214GlyMet: 2.214 ± 0.426
2.749GlyAsn: 2.749 ± 0.488
2.214GlyPro: 2.214 ± 0.397
3.971GlyGln: 3.971 ± 0.561
4.734GlyArg: 4.734 ± 0.615
4.047GlySer: 4.047 ± 0.738
4.2GlyThr: 4.2 ± 0.642
5.651GlyVal: 5.651 ± 0.603
2.062GlyTrp: 2.062 ± 0.358
2.52GlyTyr: 2.52 ± 0.435
0.0GlyXaa: 0.0 ± 0.0
His
1.833HisAla: 1.833 ± 0.405
0.076HisCys: 0.076 ± 0.071
0.84HisAsp: 0.84 ± 0.224
1.069HisGlu: 1.069 ± 0.246
0.305HisPhe: 0.305 ± 0.126
1.68HisGly: 1.68 ± 0.362
0.305HisHis: 0.305 ± 0.152
1.069HisIle: 1.069 ± 0.268
0.611HisLys: 0.611 ± 0.179
2.138HisLeu: 2.138 ± 0.454
0.153HisMet: 0.153 ± 0.111
0.764HisAsn: 0.764 ± 0.261
1.222HisPro: 1.222 ± 0.323
1.222HisGln: 1.222 ± 0.355
1.298HisArg: 1.298 ± 0.277
1.374HisSer: 1.374 ± 0.377
0.764HisThr: 0.764 ± 0.264
1.069HisVal: 1.069 ± 0.285
0.229HisTrp: 0.229 ± 0.129
0.229HisTyr: 0.229 ± 0.149
0.0HisXaa: 0.0 ± 0.0
Ile
5.956IleAla: 5.956 ± 0.863
0.305IleCys: 0.305 ± 0.168
2.673IleAsp: 2.673 ± 0.389
4.276IleGlu: 4.276 ± 0.569
0.84IlePhe: 0.84 ± 0.25
2.367IleGly: 2.367 ± 0.415
0.993IleHis: 0.993 ± 0.314
1.222IleIle: 1.222 ± 0.377
1.298IleLys: 1.298 ± 0.287
3.054IleLeu: 3.054 ± 0.436
0.458IleMet: 0.458 ± 0.194
1.604IleAsn: 1.604 ± 0.446
1.756IlePro: 1.756 ± 0.327
1.756IleGln: 1.756 ± 0.36
3.207IleArg: 3.207 ± 0.481
2.825IleSer: 2.825 ± 0.56
3.207IleThr: 3.207 ± 0.555
1.68IleVal: 1.68 ± 0.297
0.764IleTrp: 0.764 ± 0.364
0.84IleTyr: 0.84 ± 0.274
0.0IleXaa: 0.0 ± 0.0
Lys
6.261LysAla: 6.261 ± 0.846
0.229LysCys: 0.229 ± 0.135
2.062LysAsp: 2.062 ± 0.42
2.443LysGlu: 2.443 ± 0.458
0.84LysPhe: 0.84 ± 0.173
2.902LysGly: 2.902 ± 0.553
0.535LysHis: 0.535 ± 0.183
1.298LysIle: 1.298 ± 0.314
1.604LysLys: 1.604 ± 0.281
3.742LysLeu: 3.742 ± 0.552
0.305LysMet: 0.305 ± 0.149
1.069LysAsn: 1.069 ± 0.296
2.062LysPro: 2.062 ± 0.616
1.222LysGln: 1.222 ± 0.391
4.2LysArg: 4.2 ± 0.682
2.367LysSer: 2.367 ± 0.43
1.985LysThr: 1.985 ± 0.413
2.138LysVal: 2.138 ± 0.433
0.458LysTrp: 0.458 ± 0.234
0.611LysTyr: 0.611 ± 0.168
0.0LysXaa: 0.0 ± 0.0
Leu
12.599LeuAla: 12.599 ± 0.951
0.993LeuCys: 0.993 ± 0.317
6.949LeuAsp: 6.949 ± 0.754
5.574LeuGlu: 5.574 ± 0.677
2.596LeuPhe: 2.596 ± 0.596
7.025LeuGly: 7.025 ± 0.6
2.138LeuHis: 2.138 ± 0.36
4.276LeuIle: 4.276 ± 0.59
3.665LeuLys: 3.665 ± 0.632
9.239LeuLeu: 9.239 ± 0.988
2.214LeuMet: 2.214 ± 0.324
3.283LeuAsn: 3.283 ± 0.46
5.422LeuPro: 5.422 ± 0.668
5.574LeuGln: 5.574 ± 0.897
8.018LeuArg: 8.018 ± 0.746
5.956LeuSer: 5.956 ± 0.583
6.032LeuThr: 6.032 ± 0.704
7.865LeuVal: 7.865 ± 0.702
0.611LeuTrp: 0.611 ± 0.191
2.062LeuTyr: 2.062 ± 0.475
0.0LeuXaa: 0.0 ± 0.0
Met
3.131MetAla: 3.131 ± 0.45
0.0MetCys: 0.0 ± 0.0
1.222MetAsp: 1.222 ± 0.278
1.374MetGlu: 1.374 ± 0.329
0.611MetPhe: 0.611 ± 0.183
2.214MetGly: 2.214 ± 0.367
0.382MetHis: 0.382 ± 0.186
1.069MetIle: 1.069 ± 0.29
0.993MetLys: 0.993 ± 0.306
1.833MetLeu: 1.833 ± 0.501
0.076MetMet: 0.076 ± 0.075
0.916MetAsn: 0.916 ± 0.32
0.993MetPro: 0.993 ± 0.252
1.145MetGln: 1.145 ± 0.273
1.145MetArg: 1.145 ± 0.242
2.062MetSer: 2.062 ± 0.361
1.604MetThr: 1.604 ± 0.405
1.145MetVal: 1.145 ± 0.267
0.305MetTrp: 0.305 ± 0.212
0.0MetTyr: 0.0 ± 0.0
0.0MetXaa: 0.0 ± 0.0
Asn
3.436AsnAla: 3.436 ± 0.487
0.305AsnCys: 0.305 ± 0.263
1.298AsnAsp: 1.298 ± 0.3
1.756AsnGlu: 1.756 ± 0.31
0.687AsnPhe: 0.687 ± 0.231
3.131AsnGly: 3.131 ± 0.685
0.687AsnHis: 0.687 ± 0.204
0.535AsnIle: 0.535 ± 0.187
0.84AsnLys: 0.84 ± 0.228
3.207AsnLeu: 3.207 ± 0.424
0.458AsnMet: 0.458 ± 0.185
0.687AsnAsn: 0.687 ± 0.293
1.756AsnPro: 1.756 ± 0.392
1.833AsnGln: 1.833 ± 0.389
2.367AsnArg: 2.367 ± 0.424
1.069AsnSer: 1.069 ± 0.24
1.833AsnThr: 1.833 ± 0.394
1.604AsnVal: 1.604 ± 0.27
0.687AsnTrp: 0.687 ± 0.221
0.993AsnTyr: 0.993 ± 0.268
0.0AsnXaa: 0.0 ± 0.0
Pro
6.567ProAla: 6.567 ± 0.67
0.229ProCys: 0.229 ± 0.131
3.283ProAsp: 3.283 ± 0.605
2.443ProGlu: 2.443 ± 0.398
2.214ProPhe: 2.214 ± 0.451
4.123ProGly: 4.123 ± 0.629
0.458ProHis: 0.458 ± 0.167
1.604ProIle: 1.604 ± 0.27
1.298ProLys: 1.298 ± 0.345
3.818ProLeu: 3.818 ± 0.645
1.298ProMet: 1.298 ± 0.25
1.069ProAsn: 1.069 ± 0.27
2.367ProPro: 2.367 ± 0.547
1.604ProGln: 1.604 ± 0.271
3.971ProArg: 3.971 ± 0.463
3.131ProSer: 3.131 ± 0.469
2.52ProThr: 2.52 ± 0.4
3.818ProVal: 3.818 ± 0.613
0.916ProTrp: 0.916 ± 0.284
1.833ProTyr: 1.833 ± 0.451
0.0ProXaa: 0.0 ± 0.0
Gln
7.33GlnAla: 7.33 ± 1.03
0.305GlnCys: 0.305 ± 0.137
2.214GlnAsp: 2.214 ± 0.438
3.054GlnGlu: 3.054 ± 0.651
1.985GlnPhe: 1.985 ± 0.349
2.062GlnGly: 2.062 ± 0.425
0.84GlnHis: 0.84 ± 0.239
1.604GlnIle: 1.604 ± 0.253
1.909GlnLys: 1.909 ± 0.682
6.796GlnLeu: 6.796 ± 0.773
0.916GlnMet: 0.916 ± 0.259
0.84GlnAsn: 0.84 ± 0.304
3.513GlnPro: 3.513 ± 0.488
3.207GlnGln: 3.207 ± 0.646
3.665GlnArg: 3.665 ± 0.467
1.985GlnSer: 1.985 ± 0.386
2.367GlnThr: 2.367 ± 0.383
3.665GlnVal: 3.665 ± 0.348
0.764GlnTrp: 0.764 ± 0.215
1.298GlnTyr: 1.298 ± 0.341
0.0GlnXaa: 0.0 ± 0.0
Arg
7.254ArgAla: 7.254 ± 0.753
0.687ArgCys: 0.687 ± 0.187
4.505ArgAsp: 4.505 ± 0.496
4.276ArgGlu: 4.276 ± 0.631
1.985ArgPhe: 1.985 ± 0.409
4.963ArgGly: 4.963 ± 0.647
1.604ArgHis: 1.604 ± 0.367
3.742ArgIle: 3.742 ± 0.651
3.283ArgLys: 3.283 ± 0.743
8.323ArgLeu: 8.323 ± 0.684
1.909ArgMet: 1.909 ± 0.358
1.604ArgAsn: 1.604 ± 0.406
3.054ArgPro: 3.054 ± 0.558
4.047ArgGln: 4.047 ± 0.733
5.88ArgArg: 5.88 ± 0.697
4.2ArgSer: 4.2 ± 0.568
2.52ArgThr: 2.52 ± 0.455
4.429ArgVal: 4.429 ± 0.638
1.604ArgTrp: 1.604 ± 0.41
2.596ArgTyr: 2.596 ± 0.457
0.0ArgXaa: 0.0 ± 0.0
Ser
9.01SerAla: 9.01 ± 0.818
0.535SerCys: 0.535 ± 0.213
3.436SerAsp: 3.436 ± 0.544
3.131SerGlu: 3.131 ± 0.412
2.062SerPhe: 2.062 ± 0.339
4.352SerGly: 4.352 ± 0.539
0.535SerHis: 0.535 ± 0.211
2.291SerIle: 2.291 ± 0.368
2.214SerLys: 2.214 ± 0.462
6.185SerLeu: 6.185 ± 0.56
1.451SerMet: 1.451 ± 0.393
1.985SerAsn: 1.985 ± 0.303
3.283SerPro: 3.283 ± 0.567
1.68SerGln: 1.68 ± 0.317
3.665SerArg: 3.665 ± 0.6
3.513SerSer: 3.513 ± 0.507
3.36SerThr: 3.36 ± 0.395
3.742SerVal: 3.742 ± 0.58
0.764SerTrp: 0.764 ± 0.286
1.68SerTyr: 1.68 ± 0.351
0.0SerXaa: 0.0 ± 0.0
Thr
6.414ThrAla: 6.414 ± 0.79
0.535ThrCys: 0.535 ± 0.252
2.214ThrAsp: 2.214 ± 0.407
2.978ThrGlu: 2.978 ± 0.522
1.68ThrPhe: 1.68 ± 0.31
5.803ThrGly: 5.803 ± 0.881
1.222ThrHis: 1.222 ± 0.306
1.909ThrIle: 1.909 ± 0.436
1.604ThrLys: 1.604 ± 0.388
5.116ThrLeu: 5.116 ± 0.569
1.069ThrMet: 1.069 ± 0.294
1.68ThrAsn: 1.68 ± 0.394
3.589ThrPro: 3.589 ± 0.444
1.604ThrGln: 1.604 ± 0.353
2.673ThrArg: 2.673 ± 0.33
3.894ThrSer: 3.894 ± 0.512
3.207ThrThr: 3.207 ± 0.606
3.436ThrVal: 3.436 ± 0.542
0.916ThrTrp: 0.916 ± 0.298
1.298ThrTyr: 1.298 ± 0.298
0.0ThrXaa: 0.0 ± 0.0
Val
7.101ValAla: 7.101 ± 0.722
0.611ValCys: 0.611 ± 0.191
4.505ValAsp: 4.505 ± 0.507
4.658ValGlu: 4.658 ± 0.666
1.985ValPhe: 1.985 ± 0.342
3.971ValGly: 3.971 ± 0.534
1.222ValHis: 1.222 ± 0.275
3.283ValIle: 3.283 ± 0.54
2.443ValLys: 2.443 ± 0.453
6.72ValLeu: 6.72 ± 0.77
1.451ValMet: 1.451 ± 0.313
1.604ValAsn: 1.604 ± 0.371
3.742ValPro: 3.742 ± 0.51
3.207ValGln: 3.207 ± 0.408
3.283ValArg: 3.283 ± 0.506
3.818ValSer: 3.818 ± 0.613
3.818ValThr: 3.818 ± 0.543
5.116ValVal: 5.116 ± 0.785
1.298ValTrp: 1.298 ± 0.365
0.764ValTyr: 0.764 ± 0.301
0.0ValXaa: 0.0 ± 0.0
Trp
1.451TrpAla: 1.451 ± 0.273
0.305TrpCys: 0.305 ± 0.142
0.84TrpAsp: 0.84 ± 0.286
0.305TrpGlu: 0.305 ± 0.15
0.84TrpPhe: 0.84 ± 0.217
0.993TrpGly: 0.993 ± 0.273
0.305TrpHis: 0.305 ± 0.135
1.222TrpIle: 1.222 ± 0.32
0.458TrpLys: 0.458 ± 0.156
2.138TrpLeu: 2.138 ± 0.428
0.687TrpMet: 0.687 ± 0.26
0.611TrpAsn: 0.611 ± 0.215
0.764TrpPro: 0.764 ± 0.357
1.374TrpGln: 1.374 ± 0.3
1.374TrpArg: 1.374 ± 0.251
0.916TrpSer: 0.916 ± 0.261
1.374TrpThr: 1.374 ± 0.424
1.145TrpVal: 1.145 ± 0.287
0.611TrpTrp: 0.611 ± 0.238
0.229TrpTyr: 0.229 ± 0.11
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.52TyrAla: 2.52 ± 0.422
0.153TyrCys: 0.153 ± 0.111
0.916TyrAsp: 0.916 ± 0.288
1.756TyrGlu: 1.756 ± 0.321
0.535TyrPhe: 0.535 ± 0.194
2.367TyrGly: 2.367 ± 0.401
0.535TyrHis: 0.535 ± 0.313
0.764TyrIle: 0.764 ± 0.272
0.84TyrLys: 0.84 ± 0.247
2.749TyrLeu: 2.749 ± 0.387
0.84TyrMet: 0.84 ± 0.268
0.764TyrAsn: 0.764 ± 0.242
1.527TyrPro: 1.527 ± 0.417
0.84TyrGln: 0.84 ± 0.215
2.138TyrArg: 2.138 ± 0.318
1.756TyrSer: 1.756 ± 0.423
1.374TyrThr: 1.374 ± 0.306
1.298TyrVal: 1.298 ± 0.292
0.382TyrTrp: 0.382 ± 0.175
0.305TyrTyr: 0.305 ± 0.167
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 56 proteins (13097 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski