Tepidiphilus thermophilus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Hydrogenophilalia; Hydrogenophilales; Hydrogenophilaceae; Tepidiphilus

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2181 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K6IVV7|A0A0K6IVV7_9PROT N-terminal domain of reverse transcriptase (Fragment) OS=Tepidiphilus thermophilus OX=876478 GN=Ga0061068_105160 PE=4 SV=1
MM1 pKa = 6.33TTEE4 pKa = 4.0TQTDD8 pKa = 3.6DD9 pKa = 3.53VLIFTDD15 pKa = 3.56SAAAKK20 pKa = 9.51VRR22 pKa = 11.84EE23 pKa = 4.4LIEE26 pKa = 4.44EE27 pKa = 4.25EE28 pKa = 4.39GNPDD32 pKa = 2.85LKK34 pKa = 11.14LRR36 pKa = 11.84VFVTGGGCSGFQYY49 pKa = 10.93GFTFDD54 pKa = 4.11EE55 pKa = 4.83EE56 pKa = 4.47VAEE59 pKa = 4.45DD60 pKa = 3.86DD61 pKa = 4.69AVIEE65 pKa = 4.29KK66 pKa = 10.94GGVTLLIDD74 pKa = 3.8PMSYY78 pKa = 10.31QYY80 pKa = 11.58LVGAEE85 pKa = 3.63IDD87 pKa = 3.79YY88 pKa = 11.54VEE90 pKa = 5.08GIEE93 pKa = 4.2GAQFVIRR100 pKa = 11.84NPNATTTCGCGQSFSVV116 pKa = 3.85

Molecular weight: 12.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K6ITA6|A0A0K6ITA6_9PROT DNA-binding transcriptional response regulator NtrC family contains REC AAA-type ATPase and a Fis-type DNA-binding domains OS=Tepidiphilus thermophilus OX=876478 GN=Ga0061068_103105 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.07QPSKK9 pKa = 9.35IRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.13RR14 pKa = 11.84THH16 pKa = 5.96GFLVRR21 pKa = 11.84MRR23 pKa = 11.84TRR25 pKa = 11.84GGRR28 pKa = 11.84SVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.91GRR39 pKa = 11.84HH40 pKa = 4.92RR41 pKa = 11.84LAVV44 pKa = 3.37

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2181

0

2181

701948

20

1848

321.8

35.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.659 ± 0.075

0.912 ± 0.017

4.867 ± 0.041

7.099 ± 0.053

3.524 ± 0.034

7.937 ± 0.048

2.237 ± 0.026

4.381 ± 0.04

2.952 ± 0.043

11.438 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.094 ± 0.023

2.048 ± 0.028

5.658 ± 0.039

3.367 ± 0.033

8.369 ± 0.059

4.317 ± 0.035

4.698 ± 0.033

7.588 ± 0.047

1.595 ± 0.024

2.261 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski