Amino acid dipepetide frequency for Lachnospiraceae bacterium 28-4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.016AlaAla: 8.016 ± 0.12	0.982AlaCys: 0.982 ± 0.027	4.547AlaAsp: 4.547 ± 0.067	6.243AlaGlu: 6.243 ± 0.092	2.963AlaPhe: 2.963 ± 0.06	6.662AlaGly: 6.662 ± 0.09	0.998AlaHis: 0.998 ± 0.03	4.644AlaIle: 4.644 ± 0.069	4.649AlaLys: 4.649 ± 0.072	6.772AlaLeu: 6.772 ± 0.075	2.388AlaMet: 2.388 ± 0.047	2.499AlaAsn: 2.499 ± 0.054	1.943AlaPro: 1.943 ± 0.048	2.18AlaGln: 2.18 ± 0.051	3.047AlaArg: 3.047 ± 0.049	3.931AlaSer: 3.931 ± 0.075	2.764AlaThr: 2.764 ± 0.054	6.578AlaVal: 6.578 ± 0.081	0.677AlaTrp: 0.677 ± 0.026	2.986AlaTyr: 2.986 ± 0.058	0.0AlaXaa: 0.0 ± 0.0
Cys	0.973CysAla: 0.973 ± 0.03	0.283CysCys: 0.283 ± 0.016	0.741CysAsp: 0.741 ± 0.026	0.84CysGlu: 0.84 ± 0.026	0.761CysPhe: 0.761 ± 0.023	1.553CysGly: 1.553 ± 0.043	0.301CysHis: 0.301 ± 0.015	1.089CysIle: 1.089 ± 0.03	0.874CysLys: 0.874 ± 0.03	1.246CysLeu: 1.246 ± 0.036	0.503CysMet: 0.503 ± 0.02	0.56CysAsn: 0.56 ± 0.022	0.621CysPro: 0.621 ± 0.028	0.414CysGln: 0.414 ± 0.019	0.904CysArg: 0.904 ± 0.027	0.922CysSer: 0.922 ± 0.028	0.637CysThr: 0.637 ± 0.027	0.942CysVal: 0.942 ± 0.029	0.129CysTrp: 0.129 ± 0.011	0.603CysTyr: 0.603 ± 0.024	0.0CysXaa: 0.0 ± 0.0
Asp	4.016AspAla: 4.016 ± 0.066	0.78AspCys: 0.78 ± 0.026	2.66AspAsp: 2.66 ± 0.06	4.396AspGlu: 4.396 ± 0.065	2.684AspPhe: 2.684 ± 0.047	4.567AspGly: 4.567 ± 0.082	0.861AspHis: 0.861 ± 0.032	4.711AspIle: 4.711 ± 0.068	3.587AspLys: 3.587 ± 0.061	4.306AspLeu: 4.306 ± 0.059	2.07AspMet: 2.07 ± 0.033	2.102AspAsn: 2.102 ± 0.043	1.481AspPro: 1.481 ± 0.037	1.11AspGln: 1.11 ± 0.031	2.747AspArg: 2.747 ± 0.049	2.947AspSer: 2.947 ± 0.063	2.757AspThr: 2.757 ± 0.05	3.463AspVal: 3.463 ± 0.057	0.61AspTrp: 0.61 ± 0.024	2.805AspTyr: 2.805 ± 0.051	0.0AspXaa: 0.0 ± 0.0
Glu	6.161GluAla: 6.161 ± 0.092	0.941GluCys: 0.941 ± 0.031	4.581GluAsp: 4.581 ± 0.064	9.115GluGlu: 9.115 ± 0.131	2.676GluPhe: 2.676 ± 0.047	5.227GluGly: 5.227 ± 0.075	1.23GluHis: 1.23 ± 0.034	6.011GluIle: 6.011 ± 0.072	7.648GluLys: 7.648 ± 0.094	6.923GluLeu: 6.923 ± 0.088	2.751GluMet: 2.751 ± 0.045	4.408GluAsn: 4.408 ± 0.068	1.989GluPro: 1.989 ± 0.045	2.963GluGln: 2.963 ± 0.064	4.311GluArg: 4.311 ± 0.069	3.535GluSer: 3.535 ± 0.057	3.827GluThr: 3.827 ± 0.06	4.379GluVal: 4.379 ± 0.064	0.869GluTrp: 0.869 ± 0.029	3.487GluTyr: 3.487 ± 0.056	0.0GluXaa: 0.0 ± 0.0
Phe	3.04PheAla: 3.04 ± 0.053	0.794PheCys: 0.794 ± 0.031	2.472PheAsp: 2.472 ± 0.047	2.619PheGlu: 2.619 ± 0.042	1.966PhePhe: 1.966 ± 0.048	2.959PheGly: 2.959 ± 0.052	0.956PheHis: 0.956 ± 0.03	2.857PheIle: 2.857 ± 0.054	1.853PheLys: 1.853 ± 0.047	4.119PheLeu: 4.119 ± 0.077	1.29PheMet: 1.29 ± 0.032	1.387PheAsn: 1.387 ± 0.036	1.38PhePro: 1.38 ± 0.034	1.393PheGln: 1.393 ± 0.03	2.026PheArg: 2.026 ± 0.045	3.023PheSer: 3.023 ± 0.051	2.125PheThr: 2.125 ± 0.043	2.625PheVal: 2.625 ± 0.051	0.464PheTrp: 0.464 ± 0.023	1.933PheTyr: 1.933 ± 0.041	0.0PheXaa: 0.0 ± 0.0
Gly	4.806GlyAla: 4.806 ± 0.077	1.255GlyCys: 1.255 ± 0.039	3.359GlyAsp: 3.359 ± 0.058	5.608GlyGlu: 5.608 ± 0.079	3.123GlyPhe: 3.123 ± 0.056	5.204GlyGly: 5.204 ± 0.106	1.171GlyHis: 1.171 ± 0.034	6.493GlyIle: 6.493 ± 0.077	6.176GlyLys: 6.176 ± 0.067	5.903GlyLeu: 5.903 ± 0.079	2.786GlyMet: 2.786 ± 0.051	3.589GlyAsn: 3.589 ± 0.069	1.028GlyPro: 1.028 ± 0.033	2.19GlyGln: 2.19 ± 0.042	3.719GlyArg: 3.719 ± 0.067	4.128GlySer: 4.128 ± 0.081	3.957GlyThr: 3.957 ± 0.075	4.549GlyVal: 4.549 ± 0.064	0.782GlyTrp: 0.782 ± 0.027	3.397GlyTyr: 3.397 ± 0.059	0.0GlyXaa: 0.0 ± 0.0
His	1.051HisAla: 1.051 ± 0.024	0.32HisCys: 0.32 ± 0.017	0.831HisAsp: 0.831 ± 0.022	1.041HisGlu: 1.041 ± 0.033	0.823HisPhe: 0.823 ± 0.024	1.213HisGly: 1.213 ± 0.031	0.397HisHis: 0.397 ± 0.025	1.516HisIle: 1.516 ± 0.036	0.993HisLys: 0.993 ± 0.029	1.484HisLeu: 1.484 ± 0.037	0.596HisMet: 0.596 ± 0.02	0.726HisAsn: 0.726 ± 0.025	0.795HisPro: 0.795 ± 0.028	0.488HisGln: 0.488 ± 0.022	0.817HisArg: 0.817 ± 0.026	0.981HisSer: 0.981 ± 0.028	0.889HisThr: 0.889 ± 0.032	1.009HisVal: 1.009 ± 0.028	0.166HisTrp: 0.166 ± 0.013	0.81HisTyr: 0.81 ± 0.031	0.0HisXaa: 0.0 ± 0.0
Ile	5.621IleAla: 5.621 ± 0.072	1.361IleCys: 1.361 ± 0.037	4.12IleAsp: 4.12 ± 0.066	5.011IleGlu: 5.011 ± 0.066	3.137IlePhe: 3.137 ± 0.061	5.277IleGly: 5.277 ± 0.077	1.395IleHis: 1.395 ± 0.037	4.914IleIle: 4.914 ± 0.081	4.168IleLys: 4.168 ± 0.064	6.78IleLeu: 6.78 ± 0.084	2.081IleMet: 2.081 ± 0.047	2.891IleAsn: 2.891 ± 0.057	3.055IlePro: 3.055 ± 0.056	2.167IleGln: 2.167 ± 0.043	4.1IleArg: 4.1 ± 0.062	4.81IleSer: 4.81 ± 0.074	3.735IleThr: 3.735 ± 0.062	4.837IleVal: 4.837 ± 0.06	0.687IleTrp: 0.687 ± 0.026	3.022IleTyr: 3.022 ± 0.051	0.0IleXaa: 0.0 ± 0.0
Lys	5.169LysAla: 5.169 ± 0.081	0.764LysCys: 0.764 ± 0.025	3.844LysAsp: 3.844 ± 0.065	7.523LysGlu: 7.523 ± 0.091	1.798LysPhe: 1.798 ± 0.04	4.728LysGly: 4.728 ± 0.067	0.95LysHis: 0.95 ± 0.029	4.946LysIle: 4.946 ± 0.063	6.425LysLys: 6.425 ± 0.093	5.197LysLeu: 5.197 ± 0.066	2.237LysMet: 2.237 ± 0.047	3.714LysAsn: 3.714 ± 0.06	2.03LysPro: 2.03 ± 0.045	2.351LysGln: 2.351 ± 0.05	3.502LysArg: 3.502 ± 0.066	3.327LysSer: 3.327 ± 0.065	3.594LysThr: 3.594 ± 0.056	3.942LysVal: 3.942 ± 0.067	0.656LysTrp: 0.656 ± 0.024	3.038LysTyr: 3.038 ± 0.051	0.0LysXaa: 0.0 ± 0.0
Leu	6.884LeuAla: 6.884 ± 0.091	1.531LeuCys: 1.531 ± 0.035	4.81LeuAsp: 4.81 ± 0.074	6.719LeuGlu: 6.719 ± 0.088	3.984LeuPhe: 3.984 ± 0.061	5.51LeuGly: 5.51 ± 0.08	1.488LeuHis: 1.488 ± 0.035	5.608LeuIle: 5.608 ± 0.088	6.048LeuLys: 6.048 ± 0.083	8.655LeuLeu: 8.655 ± 0.11	2.648LeuMet: 2.648 ± 0.05	3.68LeuAsn: 3.68 ± 0.067	3.402LeuPro: 3.402 ± 0.057	2.769LeuGln: 2.769 ± 0.05	3.834LeuArg: 3.834 ± 0.068	6.025LeuSer: 6.025 ± 0.071	4.516LeuThr: 4.516 ± 0.07	5.166LeuVal: 5.166 ± 0.074	0.876LeuTrp: 0.876 ± 0.03	3.471LeuTyr: 3.471 ± 0.057	0.0LeuXaa: 0.0 ± 0.0
Met	2.641MetAla: 2.641 ± 0.054	0.365MetCys: 0.365 ± 0.018	2.108MetAsp: 2.108 ± 0.039	3.299MetGlu: 3.299 ± 0.059	0.994MetPhe: 0.994 ± 0.032	2.302MetGly: 2.302 ± 0.046	0.512MetHis: 0.512 ± 0.022	2.197MetIle: 2.197 ± 0.043	2.676MetLys: 2.676 ± 0.048	2.819MetLeu: 2.819 ± 0.049	0.978MetMet: 0.978 ± 0.031	1.616MetAsn: 1.616 ± 0.033	1.209MetPro: 1.209 ± 0.035	1.084MetGln: 1.084 ± 0.033	1.411MetArg: 1.411 ± 0.036	1.664MetSer: 1.664 ± 0.034	1.617MetThr: 1.617 ± 0.032	1.982MetVal: 1.982 ± 0.044	0.227MetTrp: 0.227 ± 0.015	0.848MetTyr: 0.848 ± 0.023	0.0MetXaa: 0.0 ± 0.0
Asn	3.254AsnAla: 3.254 ± 0.057	0.6AsnCys: 0.6 ± 0.021	2.028AsnAsp: 2.028 ± 0.046	2.965AsnGlu: 2.965 ± 0.049	1.587AsnPhe: 1.587 ± 0.037	3.75AsnGly: 3.75 ± 0.066	0.8AsnHis: 0.8 ± 0.029	3.613AsnIle: 3.613 ± 0.057	2.584AsnLys: 2.584 ± 0.045	3.56AsnLeu: 3.56 ± 0.05	1.465AsnMet: 1.465 ± 0.037	1.908AsnAsn: 1.908 ± 0.047	1.832AsnPro: 1.832 ± 0.042	1.301AsnGln: 1.301 ± 0.032	2.341AsnArg: 2.341 ± 0.047	2.28AsnSer: 2.28 ± 0.048	2.212AsnThr: 2.212 ± 0.057	2.681AsnVal: 2.681 ± 0.054	0.435AsnTrp: 0.435 ± 0.021	1.954AsnTyr: 1.954 ± 0.044	0.0AsnXaa: 0.0 ± 0.0
Pro	2.405ProAla: 2.405 ± 0.055	0.431ProCys: 0.431 ± 0.02	2.147ProAsp: 2.147 ± 0.043	3.393ProGlu: 3.393 ± 0.055	1.515ProPhe: 1.515 ± 0.044	2.245ProGly: 2.245 ± 0.052	0.558ProHis: 0.558 ± 0.021	1.744ProIle: 1.744 ± 0.043	1.777ProLys: 1.777 ± 0.036	2.61ProLeu: 2.61 ± 0.048	0.837ProMet: 0.837 ± 0.025	1.136ProAsn: 1.136 ± 0.031	0.825ProPro: 0.825 ± 0.036	0.939ProGln: 0.939 ± 0.032	1.006ProArg: 1.006 ± 0.027	1.757ProSer: 1.757 ± 0.04	1.292ProThr: 1.292 ± 0.035	2.897ProVal: 2.897 ± 0.053	0.314ProTrp: 0.314 ± 0.016	1.49ProTyr: 1.49 ± 0.037	0.0ProXaa: 0.0 ± 0.0
Gln	2.228GlnAla: 2.228 ± 0.048	0.371GlnCys: 0.371 ± 0.018	1.484GlnAsp: 1.484 ± 0.041	2.886GlnGlu: 2.886 ± 0.06	1.159GlnPhe: 1.159 ± 0.027	2.02GlnGly: 2.02 ± 0.04	0.421GlnHis: 0.421 ± 0.02	2.45GlnIle: 2.45 ± 0.047	2.546GlnLys: 2.546 ± 0.053	2.548GlnLeu: 2.548 ± 0.05	1.215GlnMet: 1.215 ± 0.036	1.478GlnAsn: 1.478 ± 0.038	0.828GlnPro: 0.828 ± 0.03	1.118GlnGln: 1.118 ± 0.03	1.509GlnArg: 1.509 ± 0.04	1.659GlnSer: 1.659 ± 0.041	1.637GlnThr: 1.637 ± 0.045	1.775GlnVal: 1.775 ± 0.039	0.335GlnTrp: 0.335 ± 0.016	1.369GlnTyr: 1.369 ± 0.035	0.0GlnXaa: 0.0 ± 0.0
Arg	2.948ArgAla: 2.948 ± 0.054	0.668ArgCys: 0.668 ± 0.023	2.316ArgAsp: 2.316 ± 0.05	4.555ArgGlu: 4.555 ± 0.066	1.992ArgPhe: 1.992 ± 0.039	2.758ArgGly: 2.758 ± 0.048	0.85ArgHis: 0.85 ± 0.028	3.976ArgIle: 3.976 ± 0.057	4.202ArgLys: 4.202 ± 0.069	4.334ArgLeu: 4.334 ± 0.064	1.819ArgMet: 1.819 ± 0.044	2.329ArgAsn: 2.329 ± 0.045	1.384ArgPro: 1.384 ± 0.039	1.903ArgGln: 1.903 ± 0.043	2.639ArgArg: 2.639 ± 0.052	2.321ArgSer: 2.321 ± 0.042	2.337ArgThr: 2.337 ± 0.04	2.703ArgVal: 2.703 ± 0.047	0.44ArgTrp: 0.44 ± 0.02	2.109ArgTyr: 2.109 ± 0.044	0.0ArgXaa: 0.0 ± 0.0
Ser	4.221SerAla: 4.221 ± 0.079	0.817SerCys: 0.817 ± 0.029	2.975SerAsp: 2.975 ± 0.058	3.823SerGlu: 3.823 ± 0.057	2.771SerPhe: 2.771 ± 0.044	5.054SerGly: 5.054 ± 0.067	1.059SerHis: 1.059 ± 0.033	4.197SerIle: 4.197 ± 0.068	3.157SerLys: 3.157 ± 0.055	5.152SerLeu: 5.152 ± 0.073	1.844SerMet: 1.844 ± 0.034	2.158SerAsn: 2.158 ± 0.044	1.778SerPro: 1.778 ± 0.043	1.667SerGln: 1.667 ± 0.041	2.762SerArg: 2.762 ± 0.051	3.432SerSer: 3.432 ± 0.075	2.447SerThr: 2.447 ± 0.054	4.12SerVal: 4.12 ± 0.059	0.539SerTrp: 0.539 ± 0.023	2.511SerTyr: 2.511 ± 0.053	0.0SerXaa: 0.0 ± 0.0
Thr	4.258ThrAla: 4.258 ± 0.077	0.542ThrCys: 0.542 ± 0.022	2.993ThrAsp: 2.993 ± 0.058	4.031ThrGlu: 4.031 ± 0.064	1.865ThrPhe: 1.865 ± 0.042	4.347ThrGly: 4.347 ± 0.061	0.793ThrHis: 0.793 ± 0.031	3.558ThrIle: 3.558 ± 0.05	2.751ThrLys: 2.751 ± 0.058	4.188ThrLeu: 4.188 ± 0.062	1.322ThrMet: 1.322 ± 0.035	1.85ThrAsn: 1.85 ± 0.043	1.808ThrPro: 1.808 ± 0.044	1.286ThrGln: 1.286 ± 0.037	1.846ThrArg: 1.846 ± 0.037	2.536ThrSer: 2.536 ± 0.051	2.2ThrThr: 2.2 ± 0.05	4.093ThrVal: 4.093 ± 0.064	0.433ThrTrp: 0.433 ± 0.019	1.958ThrTyr: 1.958 ± 0.046	0.0ThrXaa: 0.0 ± 0.0
Val	4.327ValAla: 4.327 ± 0.079	1.255ValCys: 1.255 ± 0.037	3.535ValAsp: 3.535 ± 0.058	4.775ValGlu: 4.775 ± 0.068	3.078ValPhe: 3.078 ± 0.054	3.887ValGly: 3.887 ± 0.066	1.102ValHis: 1.102 ± 0.032	4.824ValIle: 4.824 ± 0.068	4.275ValLys: 4.275 ± 0.061	6.22ValLeu: 6.22 ± 0.079	2.053ValMet: 2.053 ± 0.044	2.754ValAsn: 2.754 ± 0.047	2.301ValPro: 2.301 ± 0.043	1.83ValGln: 1.83 ± 0.043	3.176ValArg: 3.176 ± 0.052	4.434ValSer: 4.434 ± 0.067	3.59ValThr: 3.59 ± 0.069	4.432ValVal: 4.432 ± 0.074	0.664ValTrp: 0.664 ± 0.026	2.773ValTyr: 2.773 ± 0.052	0.0ValXaa: 0.0 ± 0.0
Trp	0.571TrpAla: 0.571 ± 0.023	0.152TrpCys: 0.152 ± 0.011	0.563TrpAsp: 0.563 ± 0.024	0.835TrpGlu: 0.835 ± 0.03	0.435TrpPhe: 0.435 ± 0.017	0.689TrpGly: 0.689 ± 0.028	0.191TrpHis: 0.191 ± 0.012	0.781TrpIle: 0.781 ± 0.024	0.91TrpLys: 0.91 ± 0.027	0.898TrpLeu: 0.898 ± 0.031	0.372TrpMet: 0.372 ± 0.021	0.584TrpAsn: 0.584 ± 0.022	0.17TrpPro: 0.17 ± 0.014	0.391TrpGln: 0.391 ± 0.017	0.427TrpArg: 0.427 ± 0.021	0.458TrpSer: 0.458 ± 0.021	0.411TrpThr: 0.411 ± 0.019	0.509TrpVal: 0.509 ± 0.02	0.121TrpTrp: 0.121 ± 0.01	0.401TrpTyr: 0.401 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.882TyrAla: 2.882 ± 0.048	0.683TyrCys: 0.683 ± 0.024	2.546TyrAsp: 2.546 ± 0.058	3.358TyrGlu: 3.358 ± 0.055	1.97TyrPhe: 1.97 ± 0.042	3.271TyrGly: 3.271 ± 0.061	0.914TyrHis: 0.914 ± 0.033	3.041TyrIle: 3.041 ± 0.052	2.433TyrLys: 2.433 ± 0.048	3.909TyrLeu: 3.909 ± 0.061	1.289TyrMet: 1.289 ± 0.034	1.782TyrAsn: 1.782 ± 0.042	1.458TyrPro: 1.458 ± 0.035	1.494TyrGln: 1.494 ± 0.03	2.459TyrArg: 2.459 ± 0.049	2.313TyrSer: 2.313 ± 0.042	2.116TyrThr: 2.116 ± 0.046	2.604TyrVal: 2.604 ± 0.049	0.444TyrTrp: 0.444 ± 0.023	2.061TyrTyr: 2.061 ± 0.053	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3953 proteins (1220500 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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