Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8) (Yeast) (Hansenula anomala)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Saccharomycotina; Saccharomycetes; Saccharomycetales; Phaffomycetaceae; Wickerhamomyces; Wickerhamomyces anomalus

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6406 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1E3NYD3|A0A1E3NYD3_WICAA Uncharacterized protein OS=Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8) OX=683960 GN=WICANDRAFT_64249 PE=4 SV=1
MM1 pKa = 8.0PSRR4 pKa = 11.84SYY6 pKa = 10.78ISPPSSTHH14 pKa = 6.11SDD16 pKa = 3.19SNEE19 pKa = 3.66PQNLVNPIVLPPIRR33 pKa = 11.84SLFDD37 pKa = 3.17EE38 pKa = 4.73GRR40 pKa = 11.84LPFFVDD46 pKa = 3.52QVEE49 pKa = 4.28DD50 pKa = 4.09LPFGGADD57 pKa = 3.97DD58 pKa = 4.24PVAPITTHH66 pKa = 5.9RR67 pKa = 11.84HH68 pKa = 5.44DD69 pKa = 4.71PGSAFSDD76 pKa = 3.54SSSDD80 pKa = 2.94ISSIFSQDD88 pKa = 3.06SAPISPPLTASLHH101 pKa = 5.26STASSDD107 pKa = 2.89HH108 pKa = 6.82FYY110 pKa = 11.14FEE112 pKa = 4.93EE113 pKa = 5.19LPLSPPPPYY122 pKa = 10.34SEE124 pKa = 5.53LEE126 pKa = 4.28SFNLNHH132 pKa = 6.58NFTSDD137 pKa = 3.75DD138 pKa = 3.52PFNFYY143 pKa = 10.64LVEE146 pKa = 4.06EE147 pKa = 4.47VAYY150 pKa = 9.58EE151 pKa = 3.9HH152 pKa = 7.25AYY154 pKa = 10.21EE155 pKa = 4.45LAQAAYY161 pKa = 10.65NSTDD165 pKa = 3.56LDD167 pKa = 4.08LDD169 pKa = 3.84SGVIADD175 pKa = 5.64DD176 pKa = 3.74EE177 pKa = 4.44MTNAGDD183 pKa = 3.44WDD185 pKa = 4.12YY186 pKa = 12.1LMTRR190 pKa = 11.84QTTAPEE196 pKa = 3.91TSNIMEE202 pKa = 4.67RR203 pKa = 11.84YY204 pKa = 7.28YY205 pKa = 11.21AGVRR209 pKa = 11.84FLQEE213 pKa = 3.97HH214 pKa = 6.71GLQPSEE220 pKa = 4.11IVSPTTFLYY229 pKa = 10.28SQNIEE234 pKa = 3.98NQYY237 pKa = 10.83LSTSIVSSEE246 pKa = 4.12QPSTPDD252 pKa = 3.37VSQHH256 pKa = 6.47PPLDD260 pKa = 4.11APSSEE265 pKa = 4.02THH267 pKa = 6.31YY268 pKa = 10.98EE269 pKa = 4.0DD270 pKa = 3.92PTFKK274 pKa = 9.93MDD276 pKa = 3.49TPIEE280 pKa = 4.0RR281 pKa = 11.84WIEE284 pKa = 3.9DD285 pKa = 3.14ASQEE289 pKa = 4.92DD290 pKa = 5.0EE291 pKa = 4.14VTEE294 pKa = 4.45DD295 pKa = 3.53NEE297 pKa = 4.91SVKK300 pKa = 10.32TPVNSPLVDD309 pKa = 4.28DD310 pKa = 4.36YY311 pKa = 12.11VDD313 pKa = 3.42YY314 pKa = 11.82DD315 pKa = 3.22MDD317 pKa = 3.64EE318 pKa = 4.39EE319 pKa = 5.05YY320 pKa = 10.97FFF322 pKa = 6.44

Molecular weight: 36.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1E3P1N4|A0A1E3P1N4_WICAA Zn(2)-C6 fungal-type domain-containing protein OS=Wickerhamomyces anomalus (strain ATCC 58044 / CBS 1984 / NCYC 433 / NRRL Y-366-8) OX=683960 GN=WICANDRAFT_79636 PE=4 SV=1
NN1 pKa = 7.52SSTSSSTLLGMTPEE15 pKa = 3.92TARR18 pKa = 11.84RR19 pKa = 11.84NRR21 pKa = 11.84CTICQKK27 pKa = 8.75QFKK30 pKa = 9.67RR31 pKa = 11.84PSSLQTHH38 pKa = 7.29LYY40 pKa = 9.4SHH42 pKa = 6.85TGEE45 pKa = 4.61KK46 pKa = 10.34PFACTWDD53 pKa = 3.14NCGRR57 pKa = 11.84LFSVRR62 pKa = 11.84SNMIRR67 pKa = 11.84HH68 pKa = 6.14RR69 pKa = 11.84KK70 pKa = 7.35LHH72 pKa = 5.34EE73 pKa = 3.82RR74 pKa = 3.72

Molecular weight: 8.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6406

0

6406

2782713

49

3951

434.4

49.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.561 ± 0.032

0.988 ± 0.011

5.988 ± 0.025

6.751 ± 0.029

4.768 ± 0.022

5.37 ± 0.03

2.048 ± 0.011

6.983 ± 0.028

7.446 ± 0.03

9.685 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.634 ± 0.011

5.773 ± 0.03

4.359 ± 0.028

4.062 ± 0.028

4.008 ± 0.02

8.628 ± 0.045

5.607 ± 0.02

5.818 ± 0.021

1.059 ± 0.009

3.465 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski