Tenacibaculum sp. SG-28

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Tenacibaculum; unclassified Tenacibaculum

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2136 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S7TMX1|A0A2S7TMX1_9FLAO Probable transcriptional regulatory protein BSU00_02810 OS=Tenacibaculum sp. SG-28 OX=754426 GN=BSU00_02810 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 10.19PEE4 pKa = 4.81LKK6 pKa = 10.6FSDD9 pKa = 3.58ACSSNGNRR17 pKa = 11.84DD18 pKa = 3.32FQIGFRR24 pKa = 11.84YY25 pKa = 7.72NTSTFQDD32 pKa = 3.64DD33 pKa = 4.37NIFTLEE39 pKa = 4.16LSDD42 pKa = 4.93ASGSFANPVNLATVANKK59 pKa = 9.9NATFSEE65 pKa = 4.18ILVAFQLPDD74 pKa = 3.3NTNGSGYY81 pKa = 10.3KK82 pKa = 9.76IRR84 pKa = 11.84IKK86 pKa = 10.52ADD88 pKa = 3.1KK89 pKa = 10.04PRR91 pKa = 11.84TSSQEE96 pKa = 3.9MFSPASDD103 pKa = 3.3AFAAYY108 pKa = 10.67LLFSEE113 pKa = 4.88TLWINDD119 pKa = 3.13NKK121 pKa = 10.78ASEE124 pKa = 4.66TICGSGSVRR133 pKa = 11.84LTLNTDD139 pKa = 3.09EE140 pKa = 4.67TASFDD145 pKa = 3.39WFKK148 pKa = 11.5DD149 pKa = 2.78GDD151 pKa = 4.03FFATTTEE158 pKa = 3.88PFIEE162 pKa = 4.29VSEE165 pKa = 4.03QGKK168 pKa = 8.83YY169 pKa = 7.92QGKK172 pKa = 9.78INYY175 pKa = 7.93FEE177 pKa = 4.64CGYY180 pKa = 10.61VDD182 pKa = 3.57SRR184 pKa = 11.84LIDD187 pKa = 3.59VYY189 pKa = 11.32VVSTTDD195 pKa = 3.38AAIQGGNEE203 pKa = 3.77VEE205 pKa = 4.11ICSDD209 pKa = 3.06EE210 pKa = 4.5TYY212 pKa = 10.39TFEE215 pKa = 5.71ASISDD220 pKa = 3.32TSYY223 pKa = 10.12TYY225 pKa = 11.0NWYY228 pKa = 10.59LDD230 pKa = 3.84DD231 pKa = 4.48QLVASSNSSTYY242 pKa = 7.35TTPNTGQFGIYY253 pKa = 10.19YY254 pKa = 10.28LILEE258 pKa = 4.68KK259 pKa = 10.73DD260 pKa = 3.17GCEE263 pKa = 3.93AKK265 pKa = 10.81SQDD268 pKa = 3.7VEE270 pKa = 4.79LKK272 pKa = 10.52QKK274 pKa = 9.22TDD276 pKa = 3.18SAIEE280 pKa = 4.48EE281 pKa = 4.1ITGDD285 pKa = 3.43FGTFLLLPGEE295 pKa = 4.2NKK297 pKa = 10.22KK298 pKa = 11.04LEE300 pKa = 3.94ISIAPSSVSTDD311 pKa = 3.54IIWLKK316 pKa = 10.92NDD318 pKa = 2.88VVLQGVSGLSMTPNGPGIYY337 pKa = 9.47KK338 pKa = 10.48ARR340 pKa = 11.84VTEE343 pKa = 4.39TGTSCPFTQDD353 pKa = 2.2SDD355 pKa = 4.01VFEE358 pKa = 5.2LVDD361 pKa = 4.43LQSLSPSIRR370 pKa = 11.84TASDD374 pKa = 3.56YY375 pKa = 10.11VACGIDD381 pKa = 3.43QTRR384 pKa = 11.84LLMVGITATGTDD396 pKa = 3.38GNTYY400 pKa = 9.95EE401 pKa = 5.79LSDD404 pKa = 3.86TQVNDD409 pKa = 3.14YY410 pKa = 10.69VQYY413 pKa = 10.56QWYY416 pKa = 9.84KK417 pKa = 9.7GGNIINGATQMEE429 pKa = 4.56FAIASYY435 pKa = 10.37QEE437 pKa = 3.87NDD439 pKa = 3.19SYY441 pKa = 11.25TLEE444 pKa = 4.18VSSGTFSGVSDD455 pKa = 5.36AIDD458 pKa = 3.02IQVTIEE464 pKa = 4.04SPEE467 pKa = 3.93ILSSSTSNALCTDD480 pKa = 3.58GTIVYY485 pKa = 8.9TIEE488 pKa = 3.92EE489 pKa = 4.34VVADD493 pKa = 4.92FTYY496 pKa = 9.65TWFKK500 pKa = 10.89DD501 pKa = 3.45GEE503 pKa = 4.45EE504 pKa = 4.5IEE506 pKa = 4.41TDD508 pKa = 3.29TPEE511 pKa = 3.89TLEE514 pKa = 4.2VIEE517 pKa = 4.42EE518 pKa = 4.31GVYY521 pKa = 10.17FLQISGFGCVTDD533 pKa = 4.65LEE535 pKa = 5.15SITVVPFDD543 pKa = 4.28DD544 pKa = 3.94SAVEE548 pKa = 4.04VTPSEE553 pKa = 4.47VVVVLPGQNVTVTASGANTYY573 pKa = 7.81EE574 pKa = 3.94WYY576 pKa = 9.84EE577 pKa = 4.2DD578 pKa = 3.48SSGSLLSTNEE588 pKa = 3.88TLDD591 pKa = 3.27INTVGTYY598 pKa = 8.11TLIATVDD605 pKa = 3.57DD606 pKa = 4.35CEE608 pKa = 4.35VIKK611 pKa = 10.03TIEE614 pKa = 4.14AVEE617 pKa = 4.01QDD619 pKa = 3.71DD620 pKa = 4.81QIIVPNIVSPYY631 pKa = 9.61LQDD634 pKa = 4.8GINDD638 pKa = 3.31TWTLSNKK645 pKa = 9.8YY646 pKa = 9.92AFQPSVTIAIYY657 pKa = 10.07DD658 pKa = 3.69ANGVEE663 pKa = 4.28VLRR666 pKa = 11.84TNEE669 pKa = 4.43YY670 pKa = 10.89KK671 pKa = 10.63NDD673 pKa = 3.61WPNKK677 pKa = 8.57DD678 pKa = 3.91LRR680 pKa = 11.84NQQVFYY686 pKa = 10.91YY687 pKa = 10.36KK688 pKa = 10.31IIRR691 pKa = 11.84DD692 pKa = 3.7DD693 pKa = 3.77MLIKK697 pKa = 10.59AGSISVLDD705 pKa = 3.78

Molecular weight: 77.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S7TJL7|A0A2S7TJL7_9FLAO Peptide transporter OS=Tenacibaculum sp. SG-28 OX=754426 GN=BSU00_09150 PE=3 SV=1
MM1 pKa = 7.23SVQSVRR7 pKa = 11.84HH8 pKa = 4.4FFEE11 pKa = 5.25RR12 pKa = 11.84NGFNVSSRR20 pKa = 11.84LADD23 pKa = 3.79RR24 pKa = 11.84LGMRR28 pKa = 11.84AVNVRR33 pKa = 11.84LFFIYY38 pKa = 10.25ISFVTVGLSFGLYY51 pKa = 8.31LTLAFLLKK59 pKa = 10.31LKK61 pKa = 10.99DD62 pKa = 3.33MIYY65 pKa = 10.19TKK67 pKa = 9.83RR68 pKa = 11.84TSVFDD73 pKa = 3.66LL74 pKa = 4.18

Molecular weight: 8.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2136

0

2136

658611

38

3746

308.3

34.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.503 ± 0.048

0.782 ± 0.017

5.289 ± 0.056

6.5 ± 0.053

5.187 ± 0.045

6.301 ± 0.058

1.766 ± 0.026

8.177 ± 0.046

8.129 ± 0.078

9.154 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.145 ± 0.026

6.154 ± 0.042

3.265 ± 0.026

3.365 ± 0.033

3.394 ± 0.038

6.487 ± 0.042

6.049 ± 0.065

6.313 ± 0.046

0.969 ± 0.017

4.071 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski