Amino acid dipepetide frequency for Tenacibaculum sp. SG-28

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.402AlaAla: 4.402 ± 0.111
0.635AlaCys: 0.635 ± 0.03
3.182AlaAsp: 3.182 ± 0.094
3.718AlaGlu: 3.718 ± 0.083
3.16AlaPhe: 3.16 ± 0.076
4.045AlaGly: 4.045 ± 0.09
1.007AlaHis: 1.007 ± 0.038
6.18AlaIle: 6.18 ± 0.122
5.37AlaLys: 5.37 ± 0.091
6.171AlaLeu: 6.171 ± 0.128
1.568AlaMet: 1.568 ± 0.055
3.633AlaAsn: 3.633 ± 0.086
1.865AlaPro: 1.865 ± 0.055
2.211AlaGln: 2.211 ± 0.071
1.936AlaArg: 1.936 ± 0.062
4.377AlaSer: 4.377 ± 0.086
4.13AlaThr: 4.13 ± 0.111
4.394AlaVal: 4.394 ± 0.086
0.598AlaTrp: 0.598 ± 0.029
2.452AlaTyr: 2.452 ± 0.061
0.0AlaXaa: 0.0 ± 0.0
Cys
0.439CysAla: 0.439 ± 0.027
0.117CysCys: 0.117 ± 0.013
0.483CysAsp: 0.483 ± 0.027
0.443CysGlu: 0.443 ± 0.027
0.424CysPhe: 0.424 ± 0.026
0.591CysGly: 0.591 ± 0.029
0.164CysHis: 0.164 ± 0.019
0.692CysIle: 0.692 ± 0.034
0.566CysLys: 0.566 ± 0.03
0.639CysLeu: 0.639 ± 0.029
0.199CysMet: 0.199 ± 0.018
0.457CysAsn: 0.457 ± 0.029
0.32CysPro: 0.32 ± 0.03
0.175CysGln: 0.175 ± 0.017
0.203CysArg: 0.203 ± 0.018
0.665CysSer: 0.665 ± 0.032
0.463CysThr: 0.463 ± 0.032
0.439CysVal: 0.439 ± 0.025
0.059CysTrp: 0.059 ± 0.01
0.284CysTyr: 0.284 ± 0.02
0.0CysXaa: 0.0 ± 0.0
Asp
3.951AspAla: 3.951 ± 0.098
0.408AspCys: 0.408 ± 0.026
2.489AspAsp: 2.489 ± 0.092
2.94AspGlu: 2.94 ± 0.075
3.448AspPhe: 3.448 ± 0.079
3.781AspGly: 3.781 ± 0.22
0.711AspHis: 0.711 ± 0.035
4.519AspIle: 4.519 ± 0.088
4.121AspLys: 4.121 ± 0.085
4.806AspLeu: 4.806 ± 0.095
0.972AspMet: 0.972 ± 0.037
2.911AspAsn: 2.911 ± 0.095
1.593AspPro: 1.593 ± 0.053
1.294AspGln: 1.294 ± 0.055
1.78AspArg: 1.78 ± 0.052
3.199AspSer: 3.199 ± 0.07
3.401AspThr: 3.401 ± 0.115
3.506AspVal: 3.506 ± 0.085
0.633AspTrp: 0.633 ± 0.036
2.434AspTyr: 2.434 ± 0.07
0.0AspXaa: 0.0 ± 0.0
Glu
4.251GluAla: 4.251 ± 0.099
0.343GluCys: 0.343 ± 0.024
3.503GluAsp: 3.503 ± 0.073
5.352GluGlu: 5.352 ± 0.123
2.735GluPhe: 2.735 ± 0.065
3.413GluGly: 3.413 ± 0.083
1.064GluHis: 1.064 ± 0.043
5.697GluIle: 5.697 ± 0.087
6.248GluLys: 6.248 ± 0.125
5.915GluLeu: 5.915 ± 0.092
1.45GluMet: 1.45 ± 0.049
5.02GluAsn: 5.02 ± 0.083
1.371GluPro: 1.371 ± 0.042
2.243GluGln: 2.243 ± 0.059
2.328GluArg: 2.328 ± 0.054
3.161GluSer: 3.161 ± 0.062
3.545GluThr: 3.545 ± 0.074
4.379GluVal: 4.379 ± 0.095
0.572GluTrp: 0.572 ± 0.031
2.411GluTyr: 2.411 ± 0.059
0.0GluXaa: 0.0 ± 0.0
Phe
2.882PheAla: 2.882 ± 0.082
0.424PheCys: 0.424 ± 0.024
3.119PheAsp: 3.119 ± 0.084
3.091PheGlu: 3.091 ± 0.068
2.707PhePhe: 2.707 ± 0.082
3.621PheGly: 3.621 ± 0.088
0.849PheHis: 0.849 ± 0.033
4.08PheIle: 4.08 ± 0.101
3.643PheLys: 3.643 ± 0.084
4.809PheLeu: 4.809 ± 0.115
1.086PheMet: 1.086 ± 0.04
3.114PheAsn: 3.114 ± 0.085
1.611PhePro: 1.611 ± 0.054
1.564PheGln: 1.564 ± 0.051
1.702PheArg: 1.702 ± 0.06
4.247PheSer: 4.247 ± 0.095
3.381PheThr: 3.381 ± 0.073
3.078PheVal: 3.078 ± 0.076
0.58PheTrp: 0.58 ± 0.033
2.281PheTyr: 2.281 ± 0.069
0.0PheXaa: 0.0 ± 0.0
Gly
4.174GlyAla: 4.174 ± 0.088
0.615GlyCys: 0.615 ± 0.046
3.286GlyAsp: 3.286 ± 0.108
3.454GlyGlu: 3.454 ± 0.074
3.574GlyPhe: 3.574 ± 0.085
4.595GlyGly: 4.595 ± 0.128
1.017GlyHis: 1.017 ± 0.039
5.753GlyIle: 5.753 ± 0.102
5.02GlyLys: 5.02 ± 0.093
5.395GlyLeu: 5.395 ± 0.106
1.603GlyMet: 1.603 ± 0.058
3.609GlyAsn: 3.609 ± 0.086
1.278GlyPro: 1.278 ± 0.063
1.634GlyGln: 1.634 ± 0.053
1.989GlyArg: 1.989 ± 0.059
3.816GlySer: 3.816 ± 0.099
4.27GlyThr: 4.27 ± 0.17
4.417GlyVal: 4.417 ± 0.086
0.692GlyTrp: 0.692 ± 0.035
2.815GlyTyr: 2.815 ± 0.062
0.0GlyXaa: 0.0 ± 0.0
His
1.061HisAla: 1.061 ± 0.04
0.17HisCys: 0.17 ± 0.017
0.785HisAsp: 0.785 ± 0.033
0.966HisGlu: 0.966 ± 0.034
1.081HisPhe: 1.081 ± 0.039
1.008HisGly: 1.008 ± 0.036
0.452HisHis: 0.452 ± 0.029
1.418HisIle: 1.418 ± 0.051
1.379HisLys: 1.379 ± 0.048
1.751HisLeu: 1.751 ± 0.056
0.313HisMet: 0.313 ± 0.02
1.013HisAsn: 1.013 ± 0.045
0.879HisPro: 0.879 ± 0.037
0.664HisGln: 0.664 ± 0.03
0.72HisArg: 0.72 ± 0.037
1.023HisSer: 1.023 ± 0.046
1.073HisThr: 1.073 ± 0.04
0.94HisVal: 0.94 ± 0.037
0.191HisTrp: 0.191 ± 0.018
0.776HisTyr: 0.776 ± 0.035
0.0HisXaa: 0.0 ± 0.0
Ile
6.62IleAla: 6.62 ± 0.113
0.68IleCys: 0.68 ± 0.032
4.746IleAsp: 4.746 ± 0.082
5.452IleGlu: 5.452 ± 0.089
3.731IlePhe: 3.731 ± 0.09
5.401IleGly: 5.401 ± 0.111
1.546IleHis: 1.546 ± 0.047
5.838IleIle: 5.838 ± 0.107
5.692IleLys: 5.692 ± 0.104
7.514IleLeu: 7.514 ± 0.142
1.339IleMet: 1.339 ± 0.048
4.569IleAsn: 4.569 ± 0.092
3.403IlePro: 3.403 ± 0.072
2.926IleGln: 2.926 ± 0.061
2.68IleArg: 2.68 ± 0.068
5.767IleSer: 5.767 ± 0.097
5.281IleThr: 5.281 ± 0.122
5.126IleVal: 5.126 ± 0.106
0.673IleTrp: 0.673 ± 0.033
2.783IleTyr: 2.783 ± 0.072
0.0IleXaa: 0.0 ± 0.0
Lys
5.006LysAla: 5.006 ± 0.118
0.348LysCys: 0.348 ± 0.028
4.581LysAsp: 4.581 ± 0.086
6.995LysGlu: 6.995 ± 0.128
3.096LysPhe: 3.096 ± 0.088
4.707LysGly: 4.707 ± 0.086
1.458LysHis: 1.458 ± 0.048
6.6LysIle: 6.6 ± 0.115
7.618LysLys: 7.618 ± 0.139
6.681LysLeu: 6.681 ± 0.113
2.074LysMet: 2.074 ± 0.057
5.809LysAsn: 5.809 ± 0.13
2.29LysPro: 2.29 ± 0.068
2.899LysGln: 2.899 ± 0.073
3.122LysArg: 3.122 ± 0.076
4.531LysSer: 4.531 ± 0.099
4.675LysThr: 4.675 ± 0.09
4.897LysVal: 4.897 ± 0.072
0.701LysTrp: 0.701 ± 0.036
3.202LysTyr: 3.202 ± 0.083
0.0LysXaa: 0.0 ± 0.0
Leu
5.7LeuAla: 5.7 ± 0.096
0.654LeuCys: 0.654 ± 0.034
4.739LeuAsp: 4.739 ± 0.1
6.215LeuGlu: 6.215 ± 0.095
4.857LeuPhe: 4.857 ± 0.114
5.826LeuGly: 5.826 ± 0.111
2.016LeuHis: 2.016 ± 0.072
6.371LeuIle: 6.371 ± 0.134
7.285LeuLys: 7.285 ± 0.112
9.288LeuLeu: 9.288 ± 0.181
1.896LeuMet: 1.896 ± 0.057
5.023LeuAsn: 5.023 ± 0.097
3.438LeuPro: 3.438 ± 0.076
4.03LeuGln: 4.03 ± 0.096
3.237LeuArg: 3.237 ± 0.068
6.502LeuSer: 6.502 ± 0.109
4.836LeuThr: 4.836 ± 0.09
5.612LeuVal: 5.612 ± 0.113
0.768LeuTrp: 0.768 ± 0.043
3.251LeuTyr: 3.251 ± 0.077
0.0LeuXaa: 0.0 ± 0.0
Met
1.5MetAla: 1.5 ± 0.057
0.155MetCys: 0.155 ± 0.015
1.08MetAsp: 1.08 ± 0.035
1.424MetGlu: 1.424 ± 0.053
0.878MetPhe: 0.878 ± 0.036
1.326MetGly: 1.326 ± 0.051
0.486MetHis: 0.486 ± 0.028
1.292MetIle: 1.292 ± 0.047
2.035MetLys: 2.035 ± 0.055
2.01MetLeu: 2.01 ± 0.062
0.528MetMet: 0.528 ± 0.027
1.26MetAsn: 1.26 ± 0.041
0.741MetPro: 0.741 ± 0.033
1.004MetGln: 1.004 ± 0.043
0.94MetArg: 0.94 ± 0.041
1.322MetSer: 1.322 ± 0.05
1.114MetThr: 1.114 ± 0.044
1.418MetVal: 1.418 ± 0.053
0.146MetTrp: 0.146 ± 0.014
0.793MetTyr: 0.793 ± 0.035
0.0MetXaa: 0.0 ± 0.0
Asn
3.905AsnAla: 3.905 ± 0.08
0.498AsnCys: 0.498 ± 0.029
3.143AsnAsp: 3.143 ± 0.08
3.315AsnGlu: 3.315 ± 0.072
3.316AsnPhe: 3.316 ± 0.091
4.101AsnGly: 4.101 ± 0.111
0.979AsnHis: 0.979 ± 0.047
4.968AsnIle: 4.968 ± 0.1
4.642AsnLys: 4.642 ± 0.101
5.392AsnLeu: 5.392 ± 0.089
1.233AsnMet: 1.233 ± 0.049
3.893AsnAsn: 3.893 ± 0.091
2.751AsnPro: 2.751 ± 0.07
2.007AsnGln: 2.007 ± 0.061
2.111AsnArg: 2.111 ± 0.052
4.165AsnSer: 4.165 ± 0.091
4.245AsnThr: 4.245 ± 0.1
3.346AsnVal: 3.346 ± 0.064
0.679AsnTrp: 0.679 ± 0.033
2.853AsnTyr: 2.853 ± 0.073
0.0AsnXaa: 0.0 ± 0.0
Pro
1.793ProAla: 1.793 ± 0.059
0.214ProCys: 0.214 ± 0.02
1.828ProAsp: 1.828 ± 0.076
2.569ProGlu: 2.569 ± 0.064
1.708ProPhe: 1.708 ± 0.05
1.678ProGly: 1.678 ± 0.054
0.6ProHis: 0.6 ± 0.032
2.856ProIle: 2.856 ± 0.076
2.817ProLys: 2.817 ± 0.077
2.835ProLeu: 2.835 ± 0.063
0.747ProMet: 0.747 ± 0.033
2.417ProAsn: 2.417 ± 0.074
0.73ProPro: 0.73 ± 0.036
0.967ProGln: 0.967 ± 0.037
0.937ProArg: 0.937 ± 0.039
2.165ProSer: 2.165 ± 0.061
1.875ProThr: 1.875 ± 0.062
2.22ProVal: 2.22 ± 0.067
0.31ProTrp: 0.31 ± 0.023
1.386ProTyr: 1.386 ± 0.05
0.0ProXaa: 0.0 ± 0.0
Gln
1.902GlnAla: 1.902 ± 0.055
0.214GlnCys: 0.214 ± 0.019
1.6GlnAsp: 1.6 ± 0.048
2.677GlnGlu: 2.677 ± 0.06
1.609GlnPhe: 1.609 ± 0.048
1.776GlnGly: 1.776 ± 0.051
0.616GlnHis: 0.616 ± 0.03
2.68GlnIle: 2.68 ± 0.066
3.33GlnLys: 3.33 ± 0.081
3.067GlnLeu: 3.067 ± 0.085
0.814GlnMet: 0.814 ± 0.038
2.259GlnAsn: 2.259 ± 0.06
1.007GlnPro: 1.007 ± 0.04
1.476GlnGln: 1.476 ± 0.05
1.357GlnArg: 1.357 ± 0.042
1.772GlnSer: 1.772 ± 0.054
1.816GlnThr: 1.816 ± 0.054
1.948GlnVal: 1.948 ± 0.053
0.336GlnTrp: 0.336 ± 0.024
1.392GlnTyr: 1.392 ± 0.044
0.0GlnXaa: 0.0 ± 0.0
Arg
2.06ArgAla: 2.06 ± 0.064
0.196ArgCys: 0.196 ± 0.017
1.702ArgAsp: 1.702 ± 0.057
2.372ArgGlu: 2.372 ± 0.076
1.813ArgPhe: 1.813 ± 0.056
1.937ArgGly: 1.937 ± 0.06
0.572ArgHis: 0.572 ± 0.03
3.069ArgIle: 3.069 ± 0.069
3.104ArgLys: 3.104 ± 0.078
3.032ArgLeu: 3.032 ± 0.082
0.919ArgMet: 0.919 ± 0.039
2.249ArgAsn: 2.249 ± 0.062
0.99ArgPro: 0.99 ± 0.043
1.037ArgGln: 1.037 ± 0.038
1.23ArgArg: 1.23 ± 0.056
1.839ArgSer: 1.839 ± 0.052
1.839ArgThr: 1.839 ± 0.061
2.127ArgVal: 2.127 ± 0.056
0.342ArgTrp: 0.342 ± 0.023
1.512ArgTyr: 1.512 ± 0.049
0.0ArgXaa: 0.0 ± 0.0
Ser
3.702SerAla: 3.702 ± 0.077
0.706SerCys: 0.706 ± 0.033
3.428SerAsp: 3.428 ± 0.093
3.955SerGlu: 3.955 ± 0.075
3.943SerPhe: 3.943 ± 0.082
4.332SerGly: 4.332 ± 0.098
0.984SerHis: 0.984 ± 0.033
5.674SerIle: 5.674 ± 0.105
5.264SerLys: 5.264 ± 0.106
5.932SerLeu: 5.932 ± 0.103
1.297SerMet: 1.297 ± 0.044
3.919SerAsn: 3.919 ± 0.092
1.957SerPro: 1.957 ± 0.055
1.901SerGln: 1.901 ± 0.061
1.988SerArg: 1.988 ± 0.062
4.587SerSer: 4.587 ± 0.107
3.574SerThr: 3.574 ± 0.08
4.092SerVal: 4.092 ± 0.082
0.739SerTrp: 0.739 ± 0.035
2.899SerTyr: 2.899 ± 0.071
0.0SerXaa: 0.0 ± 0.0
Thr
3.975ThrAla: 3.975 ± 0.12
0.431ThrCys: 0.431 ± 0.031
3.173ThrAsp: 3.173 ± 0.129
3.609ThrGlu: 3.609 ± 0.077
3.129ThrPhe: 3.129 ± 0.067
3.864ThrGly: 3.864 ± 0.128
1.019ThrHis: 1.019 ± 0.04
5.72ThrIle: 5.72 ± 0.14
4.575ThrLys: 4.575 ± 0.093
5.32ThrLeu: 5.32 ± 0.09
1.096ThrMet: 1.096 ± 0.042
3.723ThrAsn: 3.723 ± 0.112
2.551ThrPro: 2.551 ± 0.07
1.78ThrGln: 1.78 ± 0.049
1.62ThrArg: 1.62 ± 0.049
4.004ThrSer: 4.004 ± 0.092
3.77ThrThr: 3.77 ± 0.103
3.961ThrVal: 3.961 ± 0.102
0.568ThrTrp: 0.568 ± 0.033
2.598ThrTyr: 2.598 ± 0.076
0.0ThrXaa: 0.0 ± 0.0
Val
4.62ValAla: 4.62 ± 0.092
0.562ValCys: 0.562 ± 0.031
3.269ValAsp: 3.269 ± 0.086
3.591ValGlu: 3.591 ± 0.075
3.509ValPhe: 3.509 ± 0.073
3.797ValGly: 3.797 ± 0.074
1.087ValHis: 1.087 ± 0.046
4.865ValIle: 4.865 ± 0.092
4.657ValLys: 4.657 ± 0.09
6.26ValLeu: 6.26 ± 0.1
1.312ValMet: 1.312 ± 0.052
3.541ValAsn: 3.541 ± 0.091
2.176ValPro: 2.176 ± 0.063
1.871ValGln: 1.871 ± 0.055
2.12ValArg: 2.12 ± 0.061
4.563ValSer: 4.563 ± 0.087
3.999ValThr: 3.999 ± 0.104
4.312ValVal: 4.312 ± 0.106
0.577ValTrp: 0.577 ± 0.029
2.438ValTyr: 2.438 ± 0.063
0.0ValXaa: 0.0 ± 0.0
Trp
0.521TrpAla: 0.521 ± 0.031
0.093TrpCys: 0.093 ± 0.012
0.497TrpAsp: 0.497 ± 0.029
0.58TrpGlu: 0.58 ± 0.034
0.586TrpPhe: 0.586 ± 0.03
0.616TrpGly: 0.616 ± 0.031
0.191TrpHis: 0.191 ± 0.018
0.733TrpIle: 0.733 ± 0.037
0.828TrpLys: 0.828 ± 0.035
0.891TrpLeu: 0.891 ± 0.04
0.273TrpMet: 0.273 ± 0.019
0.709TrpAsn: 0.709 ± 0.038
0.185TrpPro: 0.185 ± 0.019
0.369TrpGln: 0.369 ± 0.023
0.367TrpArg: 0.367 ± 0.025
0.589TrpSer: 0.589 ± 0.036
0.512TrpThr: 0.512 ± 0.029
0.613TrpVal: 0.613 ± 0.032
0.121TrpTrp: 0.121 ± 0.014
0.416TrpTyr: 0.416 ± 0.025
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.569TyrAla: 2.569 ± 0.063
0.36TyrCys: 0.36 ± 0.023
2.261TyrAsp: 2.261 ± 0.068
2.284TyrGlu: 2.284 ± 0.078
2.563TyrPhe: 2.563 ± 0.078
2.59TyrGly: 2.59 ± 0.064
0.844TyrHis: 0.844 ± 0.034
2.762TyrIle: 2.762 ± 0.069
3.097TyrLys: 3.097 ± 0.083
3.835TyrLeu: 3.835 ± 0.082
0.73TyrMet: 0.73 ± 0.029
2.414TyrAsn: 2.414 ± 0.066
1.518TyrPro: 1.518 ± 0.056
1.602TyrGln: 1.602 ± 0.053
1.575TyrArg: 1.575 ± 0.052
2.58TyrSer: 2.58 ± 0.065
2.686TyrThr: 2.686 ± 0.084
2.3TyrVal: 2.3 ± 0.063
0.405TyrTrp: 0.405 ± 0.028
1.729TyrTyr: 1.729 ± 0.066
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2136 proteins (658611 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski