Halomonas huangheensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Halomonadaceae; Halomonas

Average proteome isoelectric point is 5.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4209 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W1N8F4|W1N8F4_9GAMM C4-dicarboxylate ABC transporter OS=Halomonas huangheensis OX=1178482 GN=BJB45_11750 PE=4 SV=1
MM1 pKa = 6.83KK2 pKa = 9.93TSRR5 pKa = 11.84LVGTAALLAVTAFGAQSALAYY26 pKa = 9.81QAGDD30 pKa = 3.52FYY32 pKa = 11.8VRR34 pKa = 11.84GGFEE38 pKa = 3.83KK39 pKa = 10.62SDD41 pKa = 3.67PEE43 pKa = 4.43SGNGALYY50 pKa = 10.83GDD52 pKa = 5.56DD53 pKa = 3.81INVSEE58 pKa = 4.8DD59 pKa = 2.77TGFAYY64 pKa = 10.2GMGYY68 pKa = 10.5LFHH71 pKa = 7.12NKK73 pKa = 9.96LGVEE77 pKa = 4.32LNGSQTMDD85 pKa = 2.97QDD87 pKa = 3.7LTAGNTGLGGSFDD100 pKa = 3.76STPINLMLNYY110 pKa = 9.54YY111 pKa = 8.86PLGGISDD118 pKa = 4.17ARR120 pKa = 11.84VHH122 pKa = 6.8PYY124 pKa = 10.76AGIGVNYY131 pKa = 8.34THH133 pKa = 7.68FDD135 pKa = 3.67NEE137 pKa = 4.52SFNADD142 pKa = 3.76LDD144 pKa = 4.3EE145 pKa = 4.54SWGFAGQVGVDD156 pKa = 4.29LSVTDD161 pKa = 3.85NLLVGTWARR170 pKa = 11.84YY171 pKa = 9.94ADD173 pKa = 3.67VDD175 pKa = 3.85TDD177 pKa = 3.35VDD179 pKa = 3.21IDD181 pKa = 3.82GTYY184 pKa = 8.61KK185 pKa = 10.05TRR187 pKa = 11.84LDD189 pKa = 3.68IDD191 pKa = 4.02PVTVGAGVTYY201 pKa = 10.65RR202 pKa = 11.84FF203 pKa = 3.91

Molecular weight: 21.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W1N691|W1N691_9GAMM Transport permease protein OS=Halomonas huangheensis OX=1178482 GN=BJB45_04765 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.42RR12 pKa = 11.84KK13 pKa = 9.04RR14 pKa = 11.84AHH16 pKa = 6.12GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.3NGRR28 pKa = 11.84AVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.93GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4209

0

4209

1385470

29

5144

329.2

36.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.691 ± 0.039

0.969 ± 0.013

5.977 ± 0.063

6.259 ± 0.04

3.411 ± 0.028

8.224 ± 0.042

2.406 ± 0.024

4.982 ± 0.027

2.4 ± 0.027

11.173 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.64 ± 0.025

2.806 ± 0.03

4.754 ± 0.029

4.146 ± 0.029

6.752 ± 0.053

6.153 ± 0.028

5.11 ± 0.056

7.338 ± 0.037

1.475 ± 0.019

2.335 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski