Staphylococcus phage vB_SauS_JS02

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Triavirus; Staphylococcus virus JS02

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6F8Z6H0|A0A6F8Z6H0_9CAUD Virulence-associated protein E OS=Staphylococcus phage vB_SauS_JS02 OX=2726708 PE=4 SV=1
MM1 pKa = 7.06ATQRR5 pKa = 11.84QVEE8 pKa = 4.5YY9 pKa = 11.4VMSLQEE15 pKa = 3.92QLEE18 pKa = 4.35LEE20 pKa = 4.45DD21 pKa = 3.95CEE23 pKa = 5.56KK24 pKa = 10.67YY25 pKa = 9.42TDD27 pKa = 3.83EE28 pKa = 4.01QVKK31 pKa = 10.4AMSHH35 pKa = 6.03KK36 pKa = 10.06EE37 pKa = 3.72VSNVIEE43 pKa = 4.41NYY45 pKa = 9.42KK46 pKa = 10.77ASISNEE52 pKa = 3.8EE53 pKa = 4.63LYY55 pKa = 10.85DD56 pKa = 3.48EE57 pKa = 4.93CMSFGLPNCC66 pKa = 4.51

Molecular weight: 7.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6F8Z5W7|A0A6F8Z5W7_9CAUD Major tail protein2 OS=Staphylococcus phage vB_SauS_JS02 OX=2726708 PE=4 SV=1
MM1 pKa = 7.64ARR3 pKa = 11.84RR4 pKa = 11.84KK5 pKa = 9.81VIRR8 pKa = 11.84VRR10 pKa = 11.84IKK12 pKa = 10.5GKK14 pKa = 10.6LMTLRR19 pKa = 11.84EE20 pKa = 4.12VSEE23 pKa = 4.8KK24 pKa = 10.69YY25 pKa = 10.32HH26 pKa = 6.64ISPEE30 pKa = 4.02LLRR33 pKa = 11.84YY34 pKa = 9.46RR35 pKa = 11.84YY36 pKa = 8.24KK37 pKa = 10.75HH38 pKa = 6.21KK39 pKa = 10.45MRR41 pKa = 11.84GDD43 pKa = 3.41EE44 pKa = 4.19LLCGRR49 pKa = 11.84KK50 pKa = 9.44DD51 pKa = 3.52SKK53 pKa = 11.56SKK55 pKa = 11.51DD56 pKa = 3.34EE57 pKa = 4.1VEE59 pKa = 4.23YY60 pKa = 9.76MQSQIKK66 pKa = 10.45DD67 pKa = 3.49EE68 pKa = 4.33EE69 pKa = 4.2KK70 pKa = 10.52GRR72 pKa = 11.84EE73 pKa = 4.07KK74 pKa = 10.34IRR76 pKa = 11.84KK77 pKa = 8.34KK78 pKa = 10.82AILNRR83 pKa = 11.84YY84 pKa = 5.97QRR86 pKa = 11.84NVRR89 pKa = 11.84AEE91 pKa = 3.88YY92 pKa = 8.71EE93 pKa = 4.07QEE95 pKa = 3.75RR96 pKa = 11.84KK97 pKa = 9.61RR98 pKa = 11.84RR99 pKa = 11.84LRR101 pKa = 11.84PWLYY105 pKa = 10.66DD106 pKa = 3.11GTPQKK111 pKa = 10.53HH112 pKa = 6.08SRR114 pKa = 11.84DD115 pKa = 4.06PYY117 pKa = 10.08WFDD120 pKa = 3.01VTYY123 pKa = 11.33NQMFKK128 pKa = 10.23KK129 pKa = 9.86WSEE132 pKa = 3.76AA133 pKa = 3.32

Molecular weight: 16.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

14009

38

2074

209.1

23.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.839 ± 0.602

0.5 ± 0.101

6.11 ± 0.292

7.738 ± 0.422

3.662 ± 0.186

6.025 ± 0.574

1.842 ± 0.133

7.01 ± 0.376

9.915 ± 0.464

8.159 ± 0.335

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.584 ± 0.13

6.696 ± 0.315

2.72 ± 0.208

3.798 ± 0.163

4.112 ± 0.219

6.446 ± 0.352

5.661 ± 0.272

5.611 ± 0.227

1.263 ± 0.12

4.312 ± 0.361
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski