Cariama cristata (Red-legged seriema)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Sauropsida; Sauria; Archelosauria; Archosauria; Dinosauria;

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8694 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A091M2E2|A0A091M2E2_CARIC Discoidin domain-containing receptor 2 (Fragment) OS=Cariama cristata OX=54380 GN=N322_13046 PE=4 SV=1
MM1 pKa = 6.59VTGDD5 pKa = 3.95VLDD8 pKa = 4.03SCTAAEE14 pKa = 4.36LRR16 pKa = 11.84LDD18 pKa = 4.02SLYY21 pKa = 10.25LQCMDD26 pKa = 3.23SHH28 pKa = 6.78KK29 pKa = 11.29ADD31 pKa = 3.64VTDD34 pKa = 3.73VLPKK38 pKa = 10.27QEE40 pKa = 4.01IQASEE45 pKa = 4.14GQAAALDD52 pKa = 4.0AEE54 pKa = 4.74VLQTARR60 pKa = 11.84GFLGHH65 pKa = 5.74YY66 pKa = 5.48TTPKK70 pKa = 10.1VLSADD75 pKa = 3.71APQTGATEE83 pKa = 4.46CEE85 pKa = 4.16VGLPDD90 pKa = 4.38TYY92 pKa = 11.28LSPTADD98 pKa = 3.12SCEE101 pKa = 4.27NISLATVDD109 pKa = 5.36KK110 pKa = 11.1GDD112 pKa = 4.08LPHH115 pKa = 7.56SIVYY119 pKa = 10.04QNEE122 pKa = 3.61EE123 pKa = 4.41GKK125 pKa = 9.57WVTDD129 pKa = 3.51LAYY132 pKa = 9.07YY133 pKa = 10.17TSFDD137 pKa = 3.62EE138 pKa = 4.4EE139 pKa = 4.26QNLNLSEE146 pKa = 4.35DD147 pKa = 3.67DD148 pKa = 4.51KK149 pKa = 11.61INEE152 pKa = 4.01EE153 pKa = 4.77FITT156 pKa = 4.04

Molecular weight: 17.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A091MR16|A0A091MR16_CARIC Leucine-rich repeat-containing protein 73 (Fragment) OS=Cariama cristata OX=54380 GN=N322_11167 PE=4 SV=1
KKK2 pKa = 10.15RR3 pKa = 11.84QGKKK7 pKa = 8.77WQDDD11 pKa = 3.28KK12 pKa = 9.65RR13 pKa = 11.84HHH15 pKa = 5.16KKK17 pKa = 7.15RR18 pKa = 11.84QGMARR23 pKa = 11.84HHH25 pKa = 5.78KKK27 pKa = 9.28RR28 pKa = 11.84QGKKK32 pKa = 8.41RR33 pKa = 11.84QGKKK37 pKa = 8.77RR38 pKa = 11.84HHH40 pKa = 5.31KKK42 pKa = 9.29RR43 pKa = 11.84QGKKK47 pKa = 8.41RR48 pKa = 11.84QGKKK52 pKa = 8.81RR53 pKa = 11.84HHH55 pKa = 5.28Q

Molecular weight: 6.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8694

0

8694

3715725

31

4517

427.4

48.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.328 ± 0.021

2.23 ± 0.02

5.051 ± 0.02

7.233 ± 0.032

3.913 ± 0.02

5.84 ± 0.036

2.518 ± 0.013

5.049 ± 0.022

6.481 ± 0.029

9.68 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.256 ± 0.012

4.194 ± 0.019

5.244 ± 0.034

4.664 ± 0.027

5.15 ± 0.021

8.249 ± 0.037

5.442 ± 0.018

6.283 ± 0.02

1.171 ± 0.011

3.02 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski