Lactobacillus virus LP65

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Salchichonvirus

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 165 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q5ULS8|Q5ULS8_9CAUD Orf36 OS=Lactobacillus virus LP65 OX=298338 GN=orf36 PE=4 SV=1
MM1 pKa = 7.48VVIKK5 pKa = 10.17FRR7 pKa = 11.84GIPLEE12 pKa = 4.44DD13 pKa = 3.5VSDD16 pKa = 3.96IDD18 pKa = 4.78GVDD21 pKa = 3.29YY22 pKa = 10.99DD23 pKa = 4.11GKK25 pKa = 10.52FVYY28 pKa = 10.45GNYY31 pKa = 9.87VKK33 pKa = 10.79YY34 pKa = 10.4DD35 pKa = 3.67DD36 pKa = 4.19SAVIVGDD43 pKa = 3.71ALEE46 pKa = 4.31VGEE49 pKa = 4.91DD50 pKa = 3.87RR51 pKa = 11.84FWPSWWVPVDD61 pKa = 3.74PKK63 pKa = 10.86TVGQFTGVEE72 pKa = 4.25DD73 pKa = 3.86VDD75 pKa = 3.61GHH77 pKa = 6.7DD78 pKa = 3.77IYY80 pKa = 11.76VGDD83 pKa = 4.53EE84 pKa = 4.03VEE86 pKa = 4.3SWSDD90 pKa = 3.21ASEE93 pKa = 3.81LTMEE97 pKa = 4.65PKK99 pKa = 10.4LYY101 pKa = 10.46EE102 pKa = 4.04VVDD105 pKa = 3.72RR106 pKa = 11.84GLFNNPGYY114 pKa = 9.93YY115 pKa = 10.24LSIVGVPHH123 pKa = 7.62AIYY126 pKa = 10.03PNLSFSFGLYY136 pKa = 9.33RR137 pKa = 11.84VVGTIYY143 pKa = 10.82DD144 pKa = 3.47

Molecular weight: 16.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q5ULN6|Q5ULN6_9CAUD Orf78 OS=Lactobacillus virus LP65 OX=298338 GN=orf78 PE=4 SV=1
MM1 pKa = 7.56SGHH4 pKa = 6.37SKK6 pKa = 10.79DD7 pKa = 5.0IVCLWRR13 pKa = 11.84NRR15 pKa = 11.84YY16 pKa = 6.33TLWVRR21 pKa = 11.84DD22 pKa = 3.45KK23 pKa = 11.57GLGKK27 pKa = 10.82GSGKK31 pKa = 8.73WRR33 pKa = 11.84HH34 pKa = 5.3INGVRR39 pKa = 11.84VATDD43 pKa = 3.32LQKK46 pKa = 11.31

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

165

0

165

37260

30

2112

225.8

25.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.151 ± 0.345

0.636 ± 0.095

7.319 ± 0.207

5.754 ± 0.308

3.631 ± 0.133

6.355 ± 0.299

1.487 ± 0.097

6.669 ± 0.162

7.888 ± 0.321

7.713 ± 0.235

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.158 ± 0.081

6.951 ± 0.155

2.593 ± 0.136

3.245 ± 0.122

3.532 ± 0.144

8.739 ± 0.36

6.447 ± 0.299

6.702 ± 0.17

0.929 ± 0.064

5.091 ± 0.187
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski