Amino acid dipepetide frequency for Mycobacterium phage Brocalys

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.065AlaAla: 14.065 ± 1.5
1.361AlaCys: 1.361 ± 0.24
7.203AlaAsp: 7.203 ± 0.637
7.146AlaGlu: 7.146 ± 0.58
2.666AlaPhe: 2.666 ± 0.415
9.131AlaGly: 9.131 ± 1.136
2.666AlaHis: 2.666 ± 0.426
4.764AlaIle: 4.764 ± 0.562
4.197AlaLys: 4.197 ± 0.512
7.883AlaLeu: 7.883 ± 0.768
1.701AlaMet: 1.701 ± 0.304
3.46AlaAsn: 3.46 ± 0.437
5.275AlaPro: 5.275 ± 0.583
3.516AlaGln: 3.516 ± 0.392
7.77AlaArg: 7.77 ± 0.692
5.388AlaSer: 5.388 ± 0.708
6.239AlaThr: 6.239 ± 0.555
6.636AlaVal: 6.636 ± 0.656
2.495AlaTrp: 2.495 ± 0.408
2.212AlaTyr: 2.212 ± 0.417
0.0AlaXaa: 0.0 ± 0.0
Cys
1.078CysAla: 1.078 ± 0.273
0.0CysCys: 0.0 ± 0.0
1.361CysAsp: 1.361 ± 0.321
0.737CysGlu: 0.737 ± 0.203
0.227CysPhe: 0.227 ± 0.114
1.475CysGly: 1.475 ± 0.335
0.397CysHis: 0.397 ± 0.154
0.057CysIle: 0.057 ± 0.052
0.737CysLys: 0.737 ± 0.202
0.794CysLeu: 0.794 ± 0.253
0.227CysMet: 0.227 ± 0.111
0.454CysAsn: 0.454 ± 0.163
1.248CysPro: 1.248 ± 0.282
0.284CysGln: 0.284 ± 0.125
0.681CysArg: 0.681 ± 0.197
0.737CysSer: 0.737 ± 0.254
0.964CysThr: 0.964 ± 0.249
0.737CysVal: 0.737 ± 0.21
0.284CysTrp: 0.284 ± 0.141
0.227CysTyr: 0.227 ± 0.125
0.0CysXaa: 0.0 ± 0.0
Asp
7.089AspAla: 7.089 ± 0.621
1.134AspCys: 1.134 ± 0.263
5.048AspAsp: 5.048 ± 0.523
4.254AspGlu: 4.254 ± 0.522
1.645AspPhe: 1.645 ± 0.219
7.26AspGly: 7.26 ± 0.59
1.191AspHis: 1.191 ± 0.265
2.439AspIle: 2.439 ± 0.332
1.701AspLys: 1.701 ± 0.289
6.012AspLeu: 6.012 ± 0.508
1.248AspMet: 1.248 ± 0.305
1.475AspAsn: 1.475 ± 0.298
5.161AspPro: 5.161 ± 0.527
2.552AspGln: 2.552 ± 0.326
4.877AspArg: 4.877 ± 0.566
3.573AspSer: 3.573 ± 0.468
4.537AspThr: 4.537 ± 0.516
3.97AspVal: 3.97 ± 0.514
1.531AspTrp: 1.531 ± 0.289
1.701AspTyr: 1.701 ± 0.291
0.0AspXaa: 0.0 ± 0.0
Glu
6.806GluAla: 6.806 ± 0.738
0.851GluCys: 0.851 ± 0.254
3.119GluAsp: 3.119 ± 0.377
2.949GluGlu: 2.949 ± 0.6
2.269GluPhe: 2.269 ± 0.4
3.233GluGly: 3.233 ± 0.416
1.815GluHis: 1.815 ± 0.462
2.439GluIle: 2.439 ± 0.416
2.325GluLys: 2.325 ± 0.37
5.898GluLeu: 5.898 ± 0.701
1.475GluMet: 1.475 ± 0.32
1.588GluAsn: 1.588 ± 0.275
2.892GluPro: 2.892 ± 0.51
3.233GluGln: 3.233 ± 0.391
5.275GluArg: 5.275 ± 0.672
2.949GluSer: 2.949 ± 0.466
4.083GluThr: 4.083 ± 0.637
4.197GluVal: 4.197 ± 0.473
1.531GluTrp: 1.531 ± 0.249
1.701GluTyr: 1.701 ± 0.296
0.0GluXaa: 0.0 ± 0.0
Phe
3.063PheAla: 3.063 ± 0.419
0.227PheCys: 0.227 ± 0.12
3.119PheAsp: 3.119 ± 0.645
1.304PheGlu: 1.304 ± 0.285
0.624PhePhe: 0.624 ± 0.178
2.609PheGly: 2.609 ± 0.52
0.624PheHis: 0.624 ± 0.216
1.248PheIle: 1.248 ± 0.332
0.964PheLys: 0.964 ± 0.234
2.042PheLeu: 2.042 ± 0.336
1.021PheMet: 1.021 ± 0.236
1.021PheAsn: 1.021 ± 0.237
1.418PhePro: 1.418 ± 0.306
0.964PheGln: 0.964 ± 0.278
1.815PheArg: 1.815 ± 0.407
1.361PheSer: 1.361 ± 0.272
2.325PheThr: 2.325 ± 0.289
2.439PheVal: 2.439 ± 0.369
0.681PheTrp: 0.681 ± 0.2
0.737PheTyr: 0.737 ± 0.237
0.0PheXaa: 0.0 ± 0.0
Gly
8.11GlyAla: 8.11 ± 1.17
1.248GlyCys: 1.248 ± 0.309
5.501GlyAsp: 5.501 ± 0.495
4.991GlyGlu: 4.991 ± 0.601
2.382GlyPhe: 2.382 ± 0.424
9.585GlyGly: 9.585 ± 1.852
2.155GlyHis: 2.155 ± 0.386
3.63GlyIle: 3.63 ± 0.55
2.382GlyLys: 2.382 ± 0.342
5.728GlyLeu: 5.728 ± 0.638
2.155GlyMet: 2.155 ± 0.399
2.609GlyAsn: 2.609 ± 0.433
3.913GlyPro: 3.913 ± 0.729
2.439GlyGln: 2.439 ± 0.493
6.352GlyArg: 6.352 ± 0.675
4.764GlySer: 4.764 ± 0.666
5.275GlyThr: 5.275 ± 0.637
6.239GlyVal: 6.239 ± 0.608
2.042GlyTrp: 2.042 ± 0.346
2.155GlyTyr: 2.155 ± 0.391
0.0GlyXaa: 0.0 ± 0.0
His
2.269HisAla: 2.269 ± 0.378
0.17HisCys: 0.17 ± 0.103
1.078HisAsp: 1.078 ± 0.234
1.475HisGlu: 1.475 ± 0.325
0.51HisPhe: 0.51 ± 0.157
1.248HisGly: 1.248 ± 0.279
0.851HisHis: 0.851 ± 0.268
1.701HisIle: 1.701 ± 0.281
1.078HisLys: 1.078 ± 0.283
1.418HisLeu: 1.418 ± 0.258
0.681HisMet: 0.681 ± 0.193
1.078HisAsn: 1.078 ± 0.244
1.475HisPro: 1.475 ± 0.263
0.794HisGln: 0.794 ± 0.206
2.382HisArg: 2.382 ± 0.453
0.737HisSer: 0.737 ± 0.203
1.418HisThr: 1.418 ± 0.29
1.758HisVal: 1.758 ± 0.442
0.454HisTrp: 0.454 ± 0.15
1.021HisTyr: 1.021 ± 0.201
0.0HisXaa: 0.0 ± 0.0
Ile
4.594IleAla: 4.594 ± 0.614
0.681IleCys: 0.681 ± 0.269
3.8IleAsp: 3.8 ± 0.491
3.8IleGlu: 3.8 ± 0.444
0.907IlePhe: 0.907 ± 0.211
3.573IleGly: 3.573 ± 0.447
1.361IleHis: 1.361 ± 0.311
1.361IleIle: 1.361 ± 0.244
1.134IleLys: 1.134 ± 0.304
1.928IleLeu: 1.928 ± 0.416
0.397IleMet: 0.397 ± 0.157
1.758IleAsn: 1.758 ± 0.31
2.666IlePro: 2.666 ± 0.375
1.928IleGln: 1.928 ± 0.285
2.439IleArg: 2.439 ± 0.442
2.552IleSer: 2.552 ± 0.465
3.289IleThr: 3.289 ± 0.408
3.176IleVal: 3.176 ± 0.489
1.078IleTrp: 1.078 ± 0.278
0.681IleTyr: 0.681 ± 0.202
0.0IleXaa: 0.0 ± 0.0
Lys
4.254LysAla: 4.254 ± 0.453
0.51LysCys: 0.51 ± 0.174
2.269LysAsp: 2.269 ± 0.268
1.134LysGlu: 1.134 ± 0.264
1.361LysPhe: 1.361 ± 0.221
2.666LysGly: 2.666 ± 0.346
1.191LysHis: 1.191 ± 0.297
0.964LysIle: 0.964 ± 0.249
1.191LysLys: 1.191 ± 0.338
2.949LysLeu: 2.949 ± 0.482
0.567LysMet: 0.567 ± 0.145
1.021LysAsn: 1.021 ± 0.222
2.495LysPro: 2.495 ± 0.346
1.418LysGln: 1.418 ± 0.304
2.269LysArg: 2.269 ± 0.352
1.872LysSer: 1.872 ± 0.251
2.098LysThr: 2.098 ± 0.331
2.552LysVal: 2.552 ± 0.396
0.624LysTrp: 0.624 ± 0.165
1.078LysTyr: 1.078 ± 0.283
0.0LysXaa: 0.0 ± 0.0
Leu
8.394LeuAla: 8.394 ± 0.767
0.794LeuCys: 0.794 ± 0.214
5.048LeuAsp: 5.048 ± 0.604
4.197LeuGlu: 4.197 ± 0.466
2.382LeuPhe: 2.382 ± 0.389
5.275LeuGly: 5.275 ± 0.664
1.191LeuHis: 1.191 ± 0.232
3.233LeuIle: 3.233 ± 0.469
2.042LeuLys: 2.042 ± 0.365
4.821LeuLeu: 4.821 ± 0.55
1.872LeuMet: 1.872 ± 0.358
2.949LeuAsn: 2.949 ± 0.392
5.104LeuPro: 5.104 ± 0.65
3.233LeuGln: 3.233 ± 0.465
5.104LeuArg: 5.104 ± 0.712
5.388LeuSer: 5.388 ± 0.614
6.125LeuThr: 6.125 ± 0.507
4.934LeuVal: 4.934 ± 0.591
1.248LeuTrp: 1.248 ± 0.292
2.042LeuTyr: 2.042 ± 0.427
0.0LeuXaa: 0.0 ± 0.0
Met
2.439MetAla: 2.439 ± 0.391
0.284MetCys: 0.284 ± 0.221
1.418MetAsp: 1.418 ± 0.281
0.907MetGlu: 0.907 ± 0.223
0.567MetPhe: 0.567 ± 0.218
1.304MetGly: 1.304 ± 0.229
0.17MetHis: 0.17 ± 0.08
0.907MetIle: 0.907 ± 0.22
0.624MetLys: 0.624 ± 0.199
2.098MetLeu: 2.098 ± 0.305
0.567MetMet: 0.567 ± 0.225
0.737MetAsn: 0.737 ± 0.176
1.361MetPro: 1.361 ± 0.265
0.454MetGln: 0.454 ± 0.128
1.531MetArg: 1.531 ± 0.31
2.722MetSer: 2.722 ± 0.404
2.439MetThr: 2.439 ± 0.288
1.531MetVal: 1.531 ± 0.334
0.227MetTrp: 0.227 ± 0.111
0.397MetTyr: 0.397 ± 0.154
0.0MetXaa: 0.0 ± 0.0
Asn
3.233AsnAla: 3.233 ± 0.404
0.397AsnCys: 0.397 ± 0.164
1.418AsnAsp: 1.418 ± 0.277
1.475AsnGlu: 1.475 ± 0.311
0.624AsnPhe: 0.624 ± 0.232
3.516AsnGly: 3.516 ± 0.476
0.964AsnHis: 0.964 ± 0.213
1.021AsnIle: 1.021 ± 0.329
0.737AsnLys: 0.737 ± 0.211
3.063AsnLeu: 3.063 ± 0.478
0.851AsnMet: 0.851 ± 0.198
1.701AsnAsn: 1.701 ± 0.324
2.666AsnPro: 2.666 ± 0.403
1.248AsnGln: 1.248 ± 0.316
2.098AsnArg: 2.098 ± 0.325
1.531AsnSer: 1.531 ± 0.35
1.645AsnThr: 1.645 ± 0.274
2.098AsnVal: 2.098 ± 0.315
0.624AsnTrp: 0.624 ± 0.185
0.737AsnTyr: 0.737 ± 0.195
0.0AsnXaa: 0.0 ± 0.0
Pro
5.275ProAla: 5.275 ± 0.672
0.737ProCys: 0.737 ± 0.182
4.367ProAsp: 4.367 ± 0.554
4.254ProGlu: 4.254 ± 0.492
2.325ProPhe: 2.325 ± 0.389
6.352ProGly: 6.352 ± 0.741
1.815ProHis: 1.815 ± 0.322
1.872ProIle: 1.872 ± 0.261
2.722ProLys: 2.722 ± 0.447
4.48ProLeu: 4.48 ± 0.512
1.701ProMet: 1.701 ± 0.353
2.155ProAsn: 2.155 ± 0.382
4.254ProPro: 4.254 ± 0.541
2.382ProGln: 2.382 ± 0.375
3.119ProArg: 3.119 ± 0.487
2.836ProSer: 2.836 ± 0.341
3.289ProThr: 3.289 ± 0.537
4.821ProVal: 4.821 ± 0.515
1.304ProTrp: 1.304 ± 0.268
1.248ProTyr: 1.248 ± 0.266
0.0ProXaa: 0.0 ± 0.0
Gln
4.367GlnAla: 4.367 ± 0.452
0.284GlnCys: 0.284 ± 0.147
1.588GlnAsp: 1.588 ± 0.253
1.815GlnGlu: 1.815 ± 0.308
1.191GlnPhe: 1.191 ± 0.238
2.609GlnGly: 2.609 ± 0.473
1.134GlnHis: 1.134 ± 0.216
1.872GlnIle: 1.872 ± 0.302
1.361GlnLys: 1.361 ± 0.261
3.233GlnLeu: 3.233 ± 0.36
0.567GlnMet: 0.567 ± 0.178
0.737GlnAsn: 0.737 ± 0.24
2.609GlnPro: 2.609 ± 0.394
1.134GlnGln: 1.134 ± 0.23
2.722GlnArg: 2.722 ± 0.481
2.042GlnSer: 2.042 ± 0.337
1.758GlnThr: 1.758 ± 0.299
3.063GlnVal: 3.063 ± 0.356
0.851GlnTrp: 0.851 ± 0.204
0.851GlnTyr: 0.851 ± 0.231
0.0GlnXaa: 0.0 ± 0.0
Arg
7.146ArgAla: 7.146 ± 0.645
1.134ArgCys: 1.134 ± 0.354
5.331ArgAsp: 5.331 ± 0.633
4.991ArgGlu: 4.991 ± 0.681
2.552ArgPhe: 2.552 ± 0.368
4.48ArgGly: 4.48 ± 0.434
1.475ArgHis: 1.475 ± 0.327
4.48ArgIle: 4.48 ± 0.559
3.063ArgLys: 3.063 ± 0.455
4.934ArgLeu: 4.934 ± 0.561
2.098ArgMet: 2.098 ± 0.346
2.269ArgAsn: 2.269 ± 0.435
3.63ArgPro: 3.63 ± 0.434
2.098ArgGln: 2.098 ± 0.329
6.466ArgArg: 6.466 ± 0.842
3.516ArgSer: 3.516 ± 0.454
3.176ArgThr: 3.176 ± 0.497
4.877ArgVal: 4.877 ± 0.618
2.325ArgTrp: 2.325 ± 0.422
1.701ArgTyr: 1.701 ± 0.339
0.0ArgXaa: 0.0 ± 0.0
Ser
5.842SerAla: 5.842 ± 0.809
0.51SerCys: 0.51 ± 0.201
3.857SerAsp: 3.857 ± 0.509
2.836SerGlu: 2.836 ± 0.349
2.155SerPhe: 2.155 ± 0.314
4.651SerGly: 4.651 ± 0.492
0.964SerHis: 0.964 ± 0.212
2.439SerIle: 2.439 ± 0.317
2.382SerLys: 2.382 ± 0.345
4.31SerLeu: 4.31 ± 0.458
1.645SerMet: 1.645 ± 0.316
1.872SerAsn: 1.872 ± 0.262
3.46SerPro: 3.46 ± 0.299
1.645SerGln: 1.645 ± 0.319
3.233SerArg: 3.233 ± 0.448
3.006SerSer: 3.006 ± 0.545
3.403SerThr: 3.403 ± 0.499
4.764SerVal: 4.764 ± 0.575
0.964SerTrp: 0.964 ± 0.225
1.475SerTyr: 1.475 ± 0.232
0.0SerXaa: 0.0 ± 0.0
Thr
6.352ThrAla: 6.352 ± 0.908
0.51ThrCys: 0.51 ± 0.193
5.161ThrAsp: 5.161 ± 0.543
3.97ThrGlu: 3.97 ± 0.43
1.701ThrPhe: 1.701 ± 0.267
6.409ThrGly: 6.409 ± 0.643
1.645ThrHis: 1.645 ± 0.282
3.63ThrIle: 3.63 ± 0.511
1.928ThrLys: 1.928 ± 0.355
4.537ThrLeu: 4.537 ± 0.553
1.475ThrMet: 1.475 ± 0.285
1.361ThrAsn: 1.361 ± 0.247
4.934ThrPro: 4.934 ± 0.694
2.042ThrGln: 2.042 ± 0.345
3.857ThrArg: 3.857 ± 0.466
3.403ThrSer: 3.403 ± 0.383
4.424ThrThr: 4.424 ± 0.724
5.161ThrVal: 5.161 ± 0.634
0.964ThrTrp: 0.964 ± 0.288
1.701ThrTyr: 1.701 ± 0.285
0.0ThrXaa: 0.0 ± 0.0
Val
6.976ValAla: 6.976 ± 0.617
1.078ValCys: 1.078 ± 0.252
4.934ValAsp: 4.934 ± 0.545
5.331ValGlu: 5.331 ± 0.487
1.872ValPhe: 1.872 ± 0.373
5.104ValGly: 5.104 ± 0.492
1.134ValHis: 1.134 ± 0.239
3.119ValIle: 3.119 ± 0.514
2.666ValLys: 2.666 ± 0.363
5.331ValLeu: 5.331 ± 0.62
1.475ValMet: 1.475 ± 0.325
2.042ValAsn: 2.042 ± 0.364
4.254ValPro: 4.254 ± 0.44
2.666ValGln: 2.666 ± 0.378
5.218ValArg: 5.218 ± 0.687
4.934ValSer: 4.934 ± 0.581
4.934ValThr: 4.934 ± 0.545
6.012ValVal: 6.012 ± 0.724
1.645ValTrp: 1.645 ± 0.24
1.758ValTyr: 1.758 ± 0.366
0.0ValXaa: 0.0 ± 0.0
Trp
2.042TrpAla: 2.042 ± 0.341
0.454TrpCys: 0.454 ± 0.129
1.191TrpAsp: 1.191 ± 0.25
0.964TrpGlu: 0.964 ± 0.336
0.851TrpPhe: 0.851 ± 0.211
1.248TrpGly: 1.248 ± 0.268
0.567TrpHis: 0.567 ± 0.174
0.907TrpIle: 0.907 ± 0.221
0.681TrpLys: 0.681 ± 0.164
1.815TrpLeu: 1.815 ± 0.341
0.681TrpMet: 0.681 ± 0.198
0.567TrpAsn: 0.567 ± 0.18
0.907TrpPro: 0.907 ± 0.301
0.907TrpGln: 0.907 ± 0.247
2.495TrpArg: 2.495 ± 0.385
1.248TrpSer: 1.248 ± 0.316
1.985TrpThr: 1.985 ± 0.341
1.531TrpVal: 1.531 ± 0.328
1.021TrpTrp: 1.021 ± 0.225
0.34TrpTyr: 0.34 ± 0.136
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.382TyrAla: 2.382 ± 0.367
0.34TyrCys: 0.34 ± 0.153
1.588TyrAsp: 1.588 ± 0.4
2.098TyrGlu: 2.098 ± 0.328
0.794TyrPhe: 0.794 ± 0.199
1.928TyrGly: 1.928 ± 0.337
0.284TyrHis: 0.284 ± 0.12
1.021TyrIle: 1.021 ± 0.221
0.567TyrLys: 0.567 ± 0.174
2.098TyrLeu: 2.098 ± 0.425
0.227TyrMet: 0.227 ± 0.1
0.794TyrAsn: 0.794 ± 0.166
1.588TyrPro: 1.588 ± 0.239
0.851TyrGln: 0.851 ± 0.226
2.042TyrArg: 2.042 ± 0.392
0.851TyrSer: 0.851 ± 0.253
1.815TyrThr: 1.815 ± 0.323
1.985TyrVal: 1.985 ± 0.299
0.51TyrTrp: 0.51 ± 0.178
0.284TyrTyr: 0.284 ± 0.087
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 96 proteins (17633 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski