Citrus sinensis (Sweet orange) (Citrus aurantium var. sinensis)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Sapindales; Rutaceae; Aurantioideae;

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 44004 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A067G4T7|A0A067G4T7_CITSI Prolamin_like domain-containing protein OS=Citrus sinensis OX=2711 GN=CISIN_1g039220mg PE=4 SV=1
NN1 pKa = 7.58EE2 pKa = 3.99EE3 pKa = 3.92FGPAEE8 pKa = 4.08EE9 pKa = 4.48CCNRR13 pKa = 11.84IWKK16 pKa = 7.82PQEE19 pKa = 3.41MPEE22 pKa = 3.98YY23 pKa = 10.46LNISGDD29 pKa = 3.67PMLDD33 pKa = 3.36GYY35 pKa = 11.55SSLEE39 pKa = 3.88NSPPFPVLLTAIQAKK54 pKa = 9.72SVSDD58 pKa = 4.09SIYY61 pKa = 10.62LPVNFSSQTEE71 pKa = 4.13VVAYY75 pKa = 8.11FVIYY79 pKa = 10.4FMDD82 pKa = 5.28PIIRR86 pKa = 11.84FEE88 pKa = 3.92IGEE91 pKa = 4.11VEE93 pKa = 4.62IYY95 pKa = 10.31IDD97 pKa = 3.7GQKK100 pKa = 10.65FNARR104 pKa = 11.84DD105 pKa = 3.2VDD107 pKa = 4.0VPNWATFGTEE117 pKa = 3.84NNVVSLYY124 pKa = 9.36PVRR127 pKa = 11.84VSGSANVTISPANGSTLPPLVNAMEE152 pKa = 4.34VFQVLDD158 pKa = 3.73VVDD161 pKa = 5.49DD162 pKa = 3.99LTDD165 pKa = 3.44PP166 pKa = 4.21

Molecular weight: 18.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A067EQT9|A0A067EQT9_CITSI Isoform of A0A067EI47 Uncharacterized protein (Fragment) OS=Citrus sinensis OX=2711 GN=CISIN_1g0201162mg PE=4 SV=1
SS1 pKa = 7.41RR2 pKa = 11.84SRR4 pKa = 11.84SSSPLSSPRR13 pKa = 11.84SPRR16 pKa = 11.84GRR18 pKa = 11.84SRR20 pKa = 11.84SRR22 pKa = 11.84TRR24 pKa = 11.84SISRR28 pKa = 11.84SLSPP32 pKa = 4.29

Molecular weight: 3.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

28132

15872

44004

16339298

8

3776

371.3

41.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.789 ± 0.011

1.894 ± 0.005

5.3 ± 0.008

6.444 ± 0.013

4.277 ± 0.008

6.461 ± 0.011

2.345 ± 0.005

5.408 ± 0.009

6.085 ± 0.011

9.959 ± 0.016

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.385 ± 0.004

4.553 ± 0.009

4.778 ± 0.012

3.745 ± 0.009

5.222 ± 0.009

8.988 ± 0.016

4.751 ± 0.007

6.542 ± 0.009

1.267 ± 0.005

2.808 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski