Proteome-pI 2.0 - Proteome Isoelectric Point Database

Gordonia phage Zarbodnamra

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fairfaxidumvirus; unclassified Fairfaxidumvirus

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 84 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|A0A345MAD9|A0A345MAD9_9CAUD Minor tail protein OS=Gordonia phage Zarbodnamra OX=2283248 GN=30 PE=4 SV=1

MM1 pKa = 7.46ANDD4 pKa = 4.61TIFEE8 pKa = 4.28LPEE11 pKa = 4.65IPGVTFTASYY21 pKa = 10.84GSGGEE26 pKa = 4.12TGLPSNWIRR35 pKa = 11.84IVGTVEE41 pKa = 3.86NPWYY45 pKa = 10.31DD46 pKa = 3.23PTYY49 pKa = 11.08NYY51 pKa = 10.95GVDD54 pKa = 3.91PNGHH58 pKa = 5.62TEE60 pKa = 4.15VTDD63 pKa = 3.08PWKK66 pKa = 10.72RR67 pKa = 11.84HH68 pKa = 3.95TQFPAVLGPMGFEE81 pKa = 4.55GPSIGLPTDD90 pKa = 3.97PPPPPLEE97 pKa = 4.48PEE99 pKa = 3.97PTPDD103 pKa = 4.29IIEE106 pKa = 4.47EE107 pKa = 4.28PTDD110 pKa = 3.47GG111 pKa = 4.67

Molecular weight: 12.0 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|A0A345MAF6|A0A345MAF6_9CAUD Uncharacterized protein OS=Gordonia phage Zarbodnamra OX=2283248 GN=51 PE=4 SV=1

MM1 pKa = 6.42TTNVPIHH8 pKa = 6.57RR9 pKa = 11.84EE10 pKa = 3.47EE11 pKa = 3.84VARR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84EE17 pKa = 4.31AIAEE21 pKa = 4.11LTRR24 pKa = 11.84AGLSAPEE31 pKa = 3.47IAIRR35 pKa = 11.84LNVTARR41 pKa = 11.84TVVRR45 pKa = 11.84HH46 pKa = 5.74RR47 pKa = 11.84ALAGVSKK54 pKa = 8.77PTHH57 pKa = 5.77PRR59 pKa = 11.84LTEE62 pKa = 3.86EE63 pKa = 3.94QLATARR69 pKa = 11.84RR70 pKa = 11.84LIDD73 pKa = 3.23EE74 pKa = 4.54GAPYY78 pKa = 10.49KK79 pKa = 10.46EE80 pKa = 4.1VARR83 pKa = 11.84TIGCHH88 pKa = 5.08EE89 pKa = 4.39CTVAQRR95 pKa = 11.84FPGRR99 pKa = 11.84GWTRR103 pKa = 11.84KK104 pKa = 10.05QIDD107 pKa = 3.05EE108 pKa = 3.86WRR110 pKa = 11.84RR111 pKa = 11.84EE112 pKa = 3.77VRR114 pKa = 11.84LWADD118 pKa = 3.35ASS120 pKa = 3.72

Molecular weight: 13.66 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
84	0	84	16110	53	1840	191.8	20.9

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
11.645 ± 0.44	0.919 ± 0.155	6.89 ± 0.286	5.469 ± 0.278	2.719 ± 0.132	8.485 ± 0.334	2.16 ± 0.182	4.569 ± 0.163	3.42 ± 0.245	7.722 ± 0.255

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.216 ± 0.21	2.793 ± 0.135	5.773 ± 0.224	3.402 ± 0.145	7.449 ± 0.362	5.376 ± 0.197	6.865 ± 0.248	7.815 ± 0.205	2.135 ± 0.115	2.179 ± 0.155

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski