Amino acid dipepetide frequency for Lactococcus phage phiQ1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.142AlaAla: 2.142 ± 0.401
0.494AlaCys: 0.494 ± 0.138
2.911AlaAsp: 2.911 ± 0.398
4.394AlaGlu: 4.394 ± 0.494
2.362AlaPhe: 2.362 ± 0.448
3.955AlaGly: 3.955 ± 0.778
0.879AlaHis: 0.879 ± 0.243
4.339AlaIle: 4.339 ± 0.81
5.438AlaLys: 5.438 ± 0.542
6.042AlaLeu: 6.042 ± 0.754
2.087AlaMet: 2.087 ± 0.332
3.79AlaAsn: 3.79 ± 0.407
1.428AlaPro: 1.428 ± 0.27
2.307AlaGln: 2.307 ± 0.583
1.922AlaArg: 1.922 ± 0.362
3.735AlaSer: 3.735 ± 0.634
4.12AlaThr: 4.12 ± 0.578
4.724AlaVal: 4.724 ± 0.596
0.879AlaTrp: 0.879 ± 0.277
1.977AlaTyr: 1.977 ± 0.389
0.0AlaXaa: 0.0 ± 0.0
Cys
0.384CysAla: 0.384 ± 0.17
0.11CysCys: 0.11 ± 0.077
1.263CysAsp: 1.263 ± 0.253
0.824CysGlu: 0.824 ± 0.301
0.494CysPhe: 0.494 ± 0.155
0.879CysGly: 0.879 ± 0.248
0.22CysHis: 0.22 ± 0.111
0.659CysIle: 0.659 ± 0.183
0.989CysLys: 0.989 ± 0.284
0.989CysLeu: 0.989 ± 0.279
0.384CysMet: 0.384 ± 0.152
0.659CysAsn: 0.659 ± 0.173
0.439CysPro: 0.439 ± 0.153
0.384CysGln: 0.384 ± 0.179
0.33CysArg: 0.33 ± 0.159
0.494CysSer: 0.494 ± 0.176
0.33CysThr: 0.33 ± 0.14
0.824CysVal: 0.824 ± 0.196
0.22CysTrp: 0.22 ± 0.123
0.384CysTyr: 0.384 ± 0.179
0.0CysXaa: 0.0 ± 0.0
Asp
2.582AspAla: 2.582 ± 0.402
0.989AspCys: 0.989 ± 0.253
3.9AspAsp: 3.9 ± 0.538
5.273AspGlu: 5.273 ± 0.754
2.801AspPhe: 2.801 ± 0.353
4.394AspGly: 4.394 ± 0.495
0.824AspHis: 0.824 ± 0.171
4.614AspIle: 4.614 ± 0.547
4.614AspLys: 4.614 ± 0.505
6.591AspLeu: 6.591 ± 0.593
2.032AspMet: 2.032 ± 0.265
4.01AspAsn: 4.01 ± 0.522
1.208AspPro: 1.208 ± 0.24
1.318AspGln: 1.318 ± 0.34
2.032AspArg: 2.032 ± 0.313
4.174AspSer: 4.174 ± 0.458
4.504AspThr: 4.504 ± 0.504
4.284AspVal: 4.284 ± 0.411
1.099AspTrp: 1.099 ± 0.238
3.57AspTyr: 3.57 ± 0.514
0.0AspXaa: 0.0 ± 0.0
Glu
4.174GluAla: 4.174 ± 0.661
0.934GluCys: 0.934 ± 0.284
4.779GluAsp: 4.779 ± 0.637
5.383GluGlu: 5.383 ± 0.866
3.845GluPhe: 3.845 ± 0.563
3.57GluGly: 3.57 ± 0.409
1.813GluHis: 1.813 ± 0.329
4.284GluIle: 4.284 ± 0.562
3.735GluLys: 3.735 ± 0.505
7.305GluLeu: 7.305 ± 0.739
2.362GluMet: 2.362 ± 0.39
3.076GluAsn: 3.076 ± 0.472
2.087GluPro: 2.087 ± 0.408
2.691GluGln: 2.691 ± 0.512
3.296GluArg: 3.296 ± 0.462
3.625GluSer: 3.625 ± 0.508
4.339GluThr: 4.339 ± 0.666
5.877GluVal: 5.877 ± 0.822
0.714GluTrp: 0.714 ± 0.187
3.241GluTyr: 3.241 ± 0.491
0.0GluXaa: 0.0 ± 0.0
Phe
2.636PheAla: 2.636 ± 0.326
0.33PheCys: 0.33 ± 0.178
3.735PheAsp: 3.735 ± 0.464
2.746PheGlu: 2.746 ± 0.451
1.208PhePhe: 1.208 ± 0.29
2.746PheGly: 2.746 ± 0.357
0.384PheHis: 0.384 ± 0.154
2.966PheIle: 2.966 ± 0.511
3.186PheLys: 3.186 ± 0.4
2.472PheLeu: 2.472 ± 0.395
1.153PheMet: 1.153 ± 0.236
3.241PheAsn: 3.241 ± 0.415
0.879PhePro: 0.879 ± 0.263
1.428PheGln: 1.428 ± 0.243
0.934PheArg: 0.934 ± 0.198
2.911PheSer: 2.911 ± 0.44
3.021PheThr: 3.021 ± 0.387
2.746PheVal: 2.746 ± 0.425
0.165PheTrp: 0.165 ± 0.106
1.593PheTyr: 1.593 ± 0.321
0.0PheXaa: 0.0 ± 0.0
Gly
4.229GlyAla: 4.229 ± 1.021
0.769GlyCys: 0.769 ± 0.258
3.515GlyAsp: 3.515 ± 0.443
3.186GlyGlu: 3.186 ± 0.497
3.405GlyPhe: 3.405 ± 0.455
5.438GlyGly: 5.438 ± 1.03
0.604GlyHis: 0.604 ± 0.199
4.065GlyIle: 4.065 ± 0.65
3.79GlyLys: 3.79 ± 0.635
4.339GlyLeu: 4.339 ± 0.697
1.758GlyMet: 1.758 ± 0.319
3.9GlyAsn: 3.9 ± 0.677
0.769GlyPro: 0.769 ± 0.196
2.911GlyGln: 2.911 ± 0.403
2.252GlyArg: 2.252 ± 0.309
3.9GlySer: 3.9 ± 0.504
4.394GlyThr: 4.394 ± 0.388
4.284GlyVal: 4.284 ± 0.574
0.604GlyTrp: 0.604 ± 0.173
3.076GlyTyr: 3.076 ± 0.441
0.0GlyXaa: 0.0 ± 0.0
His
1.044HisAla: 1.044 ± 0.228
0.33HisCys: 0.33 ± 0.138
0.769HisAsp: 0.769 ± 0.262
1.153HisGlu: 1.153 ± 0.297
0.494HisPhe: 0.494 ± 0.182
0.769HisGly: 0.769 ± 0.251
0.22HisHis: 0.22 ± 0.149
1.099HisIle: 1.099 ± 0.233
1.153HisLys: 1.153 ± 0.3
1.153HisLeu: 1.153 ± 0.274
0.275HisMet: 0.275 ± 0.148
1.208HisAsn: 1.208 ± 0.258
0.824HisPro: 0.824 ± 0.229
0.604HisGln: 0.604 ± 0.178
0.989HisArg: 0.989 ± 0.265
1.044HisSer: 1.044 ± 0.231
1.263HisThr: 1.263 ± 0.308
0.934HisVal: 0.934 ± 0.218
0.165HisTrp: 0.165 ± 0.106
0.879HisTyr: 0.879 ± 0.201
0.0HisXaa: 0.0 ± 0.0
Ile
3.955IleAla: 3.955 ± 0.467
0.549IleCys: 0.549 ± 0.149
5.987IleAsp: 5.987 ± 0.606
4.12IleGlu: 4.12 ± 0.507
2.252IlePhe: 2.252 ± 0.402
3.955IleGly: 3.955 ± 0.594
0.769IleHis: 0.769 ± 0.199
3.735IleIle: 3.735 ± 0.475
5.383IleLys: 5.383 ± 0.513
4.174IleLeu: 4.174 ± 0.456
1.208IleMet: 1.208 ± 0.321
4.834IleAsn: 4.834 ± 0.506
2.252IlePro: 2.252 ± 0.428
2.801IleGln: 2.801 ± 0.366
3.076IleArg: 3.076 ± 0.411
4.559IleSer: 4.559 ± 0.489
5.108IleThr: 5.108 ± 0.608
4.339IleVal: 4.339 ± 0.469
0.604IleTrp: 0.604 ± 0.15
2.142IleTyr: 2.142 ± 0.336
0.0IleXaa: 0.0 ± 0.0
Lys
5.273LysAla: 5.273 ± 0.581
0.494LysCys: 0.494 ± 0.181
4.339LysAsp: 4.339 ± 0.43
6.536LysGlu: 6.536 ± 0.806
2.911LysPhe: 2.911 ± 0.386
3.79LysGly: 3.79 ± 0.6
1.318LysHis: 1.318 ± 0.308
3.68LysIle: 3.68 ± 0.597
4.394LysLys: 4.394 ± 0.647
6.207LysLeu: 6.207 ± 0.632
2.527LysMet: 2.527 ± 0.349
4.229LysAsn: 4.229 ± 0.427
2.142LysPro: 2.142 ± 0.428
2.801LysGln: 2.801 ± 0.41
3.351LysArg: 3.351 ± 0.484
4.394LysSer: 4.394 ± 0.524
4.449LysThr: 4.449 ± 0.544
4.229LysVal: 4.229 ± 0.566
1.099LysTrp: 1.099 ± 0.29
2.636LysTyr: 2.636 ± 0.323
0.0LysXaa: 0.0 ± 0.0
Leu
4.834LeuAla: 4.834 ± 0.505
0.934LeuCys: 0.934 ± 0.229
6.042LeuAsp: 6.042 ± 0.526
7.415LeuGlu: 7.415 ± 0.561
2.582LeuPhe: 2.582 ± 0.415
6.536LeuGly: 6.536 ± 0.888
1.703LeuHis: 1.703 ± 0.297
4.339LeuIle: 4.339 ± 0.482
6.811LeuLys: 6.811 ± 0.674
6.152LeuLeu: 6.152 ± 0.678
1.922LeuMet: 1.922 ± 0.364
6.097LeuAsn: 6.097 ± 0.566
3.351LeuPro: 3.351 ± 0.423
2.801LeuGln: 2.801 ± 0.389
3.405LeuArg: 3.405 ± 0.468
6.152LeuSer: 6.152 ± 0.513
5.328LeuThr: 5.328 ± 0.639
4.669LeuVal: 4.669 ± 0.425
0.659LeuTrp: 0.659 ± 0.2
2.911LeuTyr: 2.911 ± 0.43
0.0LeuXaa: 0.0 ± 0.0
Met
2.087MetAla: 2.087 ± 0.377
0.11MetCys: 0.11 ± 0.077
1.483MetAsp: 1.483 ± 0.269
1.758MetGlu: 1.758 ± 0.313
1.373MetPhe: 1.373 ± 0.241
1.153MetGly: 1.153 ± 0.282
0.165MetHis: 0.165 ± 0.09
1.538MetIle: 1.538 ± 0.232
1.922MetLys: 1.922 ± 0.293
2.582MetLeu: 2.582 ± 0.382
0.879MetMet: 0.879 ± 0.22
1.813MetAsn: 1.813 ± 0.252
0.934MetPro: 0.934 ± 0.241
1.099MetGln: 1.099 ± 0.216
0.494MetArg: 0.494 ± 0.154
2.307MetSer: 2.307 ± 0.288
2.856MetThr: 2.856 ± 0.445
2.362MetVal: 2.362 ± 0.335
0.11MetTrp: 0.11 ± 0.081
0.989MetTyr: 0.989 ± 0.212
0.0MetXaa: 0.0 ± 0.0
Asn
4.449AsnAla: 4.449 ± 0.599
1.208AsnCys: 1.208 ± 0.304
3.46AsnAsp: 3.46 ± 0.431
4.559AsnGlu: 4.559 ± 0.64
2.087AsnPhe: 2.087 ± 0.378
4.01AsnGly: 4.01 ± 0.439
1.593AsnHis: 1.593 ± 0.344
4.449AsnIle: 4.449 ± 0.498
4.669AsnLys: 4.669 ± 0.531
5.383AsnLeu: 5.383 ± 0.639
1.483AsnMet: 1.483 ± 0.389
3.351AsnAsn: 3.351 ± 0.453
2.966AsnPro: 2.966 ± 0.438
2.417AsnGln: 2.417 ± 0.363
2.472AsnArg: 2.472 ± 0.445
2.417AsnSer: 2.417 ± 0.386
4.888AsnThr: 4.888 ± 0.546
3.735AsnVal: 3.735 ± 0.466
0.604AsnTrp: 0.604 ± 0.165
2.417AsnTyr: 2.417 ± 0.399
0.0AsnXaa: 0.0 ± 0.0
Pro
1.813ProAla: 1.813 ± 0.375
0.22ProCys: 0.22 ± 0.123
1.922ProAsp: 1.922 ± 0.345
2.197ProGlu: 2.197 ± 0.392
1.373ProPhe: 1.373 ± 0.257
1.483ProGly: 1.483 ± 0.264
0.604ProHis: 0.604 ± 0.144
2.362ProIle: 2.362 ± 0.38
2.636ProLys: 2.636 ± 0.484
2.197ProLeu: 2.197 ± 0.312
0.494ProMet: 0.494 ± 0.141
2.032ProAsn: 2.032 ± 0.291
0.714ProPro: 0.714 ± 0.17
1.428ProGln: 1.428 ± 0.281
1.318ProArg: 1.318 ± 0.33
1.922ProSer: 1.922 ± 0.319
3.241ProThr: 3.241 ± 0.475
2.417ProVal: 2.417 ± 0.38
0.22ProTrp: 0.22 ± 0.115
1.153ProTyr: 1.153 ± 0.293
0.0ProXaa: 0.0 ± 0.0
Gln
3.46GlnAla: 3.46 ± 0.602
0.33GlnCys: 0.33 ± 0.17
1.758GlnAsp: 1.758 ± 0.301
2.746GlnGlu: 2.746 ± 0.408
2.087GlnPhe: 2.087 ± 0.369
1.758GlnGly: 1.758 ± 0.336
0.659GlnHis: 0.659 ± 0.192
2.527GlnIle: 2.527 ± 0.5
1.868GlnLys: 1.868 ± 0.383
3.79GlnLeu: 3.79 ± 0.46
1.208GlnMet: 1.208 ± 0.267
1.922GlnAsn: 1.922 ± 0.401
1.208GlnPro: 1.208 ± 0.235
1.538GlnGln: 1.538 ± 0.305
1.977GlnArg: 1.977 ± 0.355
2.582GlnSer: 2.582 ± 0.418
2.307GlnThr: 2.307 ± 0.395
3.076GlnVal: 3.076 ± 0.444
0.549GlnTrp: 0.549 ± 0.219
1.813GlnTyr: 1.813 ± 0.308
0.0GlnXaa: 0.0 ± 0.0
Arg
1.922ArgAla: 1.922 ± 0.27
0.33ArgCys: 0.33 ± 0.121
2.032ArgAsp: 2.032 ± 0.303
1.977ArgGlu: 1.977 ± 0.342
1.758ArgPhe: 1.758 ± 0.382
1.648ArgGly: 1.648 ± 0.295
0.275ArgHis: 0.275 ± 0.116
2.527ArgIle: 2.527 ± 0.498
2.966ArgLys: 2.966 ± 0.494
4.339ArgLeu: 4.339 ± 0.523
0.824ArgMet: 0.824 ± 0.201
1.813ArgAsn: 1.813 ± 0.431
1.044ArgPro: 1.044 ± 0.248
2.307ArgGln: 2.307 ± 0.358
1.758ArgArg: 1.758 ± 0.294
2.527ArgSer: 2.527 ± 0.486
2.582ArgThr: 2.582 ± 0.368
2.636ArgVal: 2.636 ± 0.378
0.494ArgTrp: 0.494 ± 0.144
1.813ArgTyr: 1.813 ± 0.346
0.0ArgXaa: 0.0 ± 0.0
Ser
3.845SerAla: 3.845 ± 0.506
0.714SerCys: 0.714 ± 0.198
4.394SerAsp: 4.394 ± 0.502
3.79SerGlu: 3.79 ± 0.515
2.417SerPhe: 2.417 ± 0.446
3.57SerGly: 3.57 ± 0.54
0.824SerHis: 0.824 ± 0.246
5.328SerIle: 5.328 ± 0.556
4.12SerLys: 4.12 ± 0.616
5.548SerLeu: 5.548 ± 0.667
1.868SerMet: 1.868 ± 0.292
4.449SerAsn: 4.449 ± 0.55
1.868SerPro: 1.868 ± 0.355
2.911SerGln: 2.911 ± 0.508
1.922SerArg: 1.922 ± 0.364
3.79SerSer: 3.79 ± 0.73
4.12SerThr: 4.12 ± 0.601
4.01SerVal: 4.01 ± 0.446
0.769SerTrp: 0.769 ± 0.244
2.582SerTyr: 2.582 ± 0.348
0.0SerXaa: 0.0 ± 0.0
Thr
3.57ThrAla: 3.57 ± 0.455
1.208ThrCys: 1.208 ± 0.298
4.559ThrAsp: 4.559 ± 0.563
4.449ThrGlu: 4.449 ± 0.561
2.527ThrPhe: 2.527 ± 0.368
4.394ThrGly: 4.394 ± 0.548
1.044ThrHis: 1.044 ± 0.211
4.724ThrIle: 4.724 ± 0.458
4.834ThrLys: 4.834 ± 0.569
6.152ThrLeu: 6.152 ± 0.689
2.252ThrMet: 2.252 ± 0.375
4.174ThrAsn: 4.174 ± 0.556
2.417ThrPro: 2.417 ± 0.402
3.021ThrGln: 3.021 ± 0.448
1.922ThrArg: 1.922 ± 0.357
4.174ThrSer: 4.174 ± 0.57
5.767ThrThr: 5.767 ± 0.764
6.152ThrVal: 6.152 ± 0.623
1.263ThrTrp: 1.263 ± 0.302
2.527ThrTyr: 2.527 ± 0.473
0.0ThrXaa: 0.0 ± 0.0
Val
4.394ValAla: 4.394 ± 0.562
0.659ValCys: 0.659 ± 0.193
4.339ValAsp: 4.339 ± 0.501
5.273ValGlu: 5.273 ± 0.76
2.142ValPhe: 2.142 ± 0.413
4.339ValGly: 4.339 ± 0.542
1.593ValHis: 1.593 ± 0.315
5.163ValIle: 5.163 ± 0.678
5.163ValLys: 5.163 ± 0.547
4.449ValLeu: 4.449 ± 0.507
1.868ValMet: 1.868 ± 0.286
4.614ValAsn: 4.614 ± 0.502
3.57ValPro: 3.57 ± 0.398
1.977ValGln: 1.977 ± 0.334
2.362ValArg: 2.362 ± 0.358
4.284ValSer: 4.284 ± 0.505
5.108ValThr: 5.108 ± 0.508
3.955ValVal: 3.955 ± 0.564
0.549ValTrp: 0.549 ± 0.189
2.362ValTyr: 2.362 ± 0.411
0.0ValXaa: 0.0 ± 0.0
Trp
0.604TrpAla: 0.604 ± 0.235
0.055TrpCys: 0.055 ± 0.054
0.33TrpAsp: 0.33 ± 0.134
1.099TrpGlu: 1.099 ± 0.234
0.549TrpPhe: 0.549 ± 0.18
0.494TrpGly: 0.494 ± 0.184
0.33TrpHis: 0.33 ± 0.137
0.824TrpIle: 0.824 ± 0.193
0.714TrpLys: 0.714 ± 0.2
1.044TrpLeu: 1.044 ± 0.276
0.384TrpMet: 0.384 ± 0.143
0.824TrpAsn: 0.824 ± 0.196
0.0TrpPro: 0.0 ± 0.0
0.494TrpGln: 0.494 ± 0.164
0.439TrpArg: 0.439 ± 0.159
0.879TrpSer: 0.879 ± 0.201
0.824TrpThr: 0.824 ± 0.278
0.549TrpVal: 0.549 ± 0.174
0.0TrpTrp: 0.0 ± 0.0
0.824TrpTyr: 0.824 ± 0.247
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.527TyrAla: 2.527 ± 0.317
0.494TyrCys: 0.494 ± 0.17
3.296TyrAsp: 3.296 ± 0.349
2.252TyrGlu: 2.252 ± 0.452
1.813TyrPhe: 1.813 ± 0.427
2.307TyrGly: 2.307 ± 0.471
0.549TyrHis: 0.549 ± 0.175
2.636TyrIle: 2.636 ± 0.391
2.417TyrLys: 2.417 ± 0.404
3.735TyrLeu: 3.735 ± 0.588
0.989TyrMet: 0.989 ± 0.228
2.582TyrAsn: 2.582 ± 0.409
1.648TyrPro: 1.648 ± 0.282
1.922TyrGln: 1.922 ± 0.45
1.318TyrArg: 1.318 ± 0.259
2.966TyrSer: 2.966 ± 0.482
2.582TyrThr: 2.582 ± 0.336
2.362TyrVal: 2.362 ± 0.278
0.494TyrTrp: 0.494 ± 0.203
2.197TyrTyr: 2.197 ± 0.443
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 86 proteins (18207 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski