Aquabacterium sp. KMB7

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiales genera incertae sedis; Aquabacterium

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3324 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4V2JFT9|A0A4V2JFT9_9BURK Heme utilization protein OS=Aquabacterium sp. KMB7 OX=2528630 GN=EYS42_04985 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.59TYY4 pKa = 10.56QCIVCGYY11 pKa = 9.88VYY13 pKa = 10.74DD14 pKa = 4.34EE15 pKa = 4.8AAGSPSEE22 pKa = 4.76GIAPGTKK29 pKa = 8.99WEE31 pKa = 4.64DD32 pKa = 3.73VPDD35 pKa = 3.42SWVCPDD41 pKa = 3.95CGVGKK46 pKa = 10.46SDD48 pKa = 4.16FEE50 pKa = 4.18MVEE53 pKa = 3.82II54 pKa = 5.08

Molecular weight: 5.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4V2JFK2|A0A4V2JFK2_9BURK Chain length-determining protein OS=Aquabacterium sp. KMB7 OX=2528630 GN=EYS42_11185 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.25QPSKK9 pKa = 7.79TRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.79GFLVRR21 pKa = 11.84MKK23 pKa = 9.67TKK25 pKa = 10.48GGRR28 pKa = 11.84KK29 pKa = 9.03VINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.44GRR39 pKa = 11.84KK40 pKa = 9.07RR41 pKa = 11.84LGLL44 pKa = 3.7

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3324

0

3324

1183161

28

6465

355.9

38.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.418 ± 0.073

0.922 ± 0.016

5.234 ± 0.036

5.06 ± 0.036

3.245 ± 0.025

8.249 ± 0.044

2.529 ± 0.031

3.8 ± 0.028

3.182 ± 0.044

10.878 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.484 ± 0.023

2.63 ± 0.034

5.398 ± 0.043

4.868 ± 0.037

6.675 ± 0.052

5.497 ± 0.041

5.454 ± 0.058

7.852 ± 0.039

1.674 ± 0.024

1.95 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski