Amino acid dipepetide frequency for Aquabacterium sp. KMB7

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.571AlaAla: 15.571 ± 0.23
1.308AlaCys: 1.308 ± 0.034
6.429AlaAsp: 6.429 ± 0.087
6.609AlaGlu: 6.609 ± 0.098
3.756AlaPhe: 3.756 ± 0.056
9.621AlaGly: 9.621 ± 0.141
3.084AlaHis: 3.084 ± 0.059
4.212AlaIle: 4.212 ± 0.078
3.481AlaLys: 3.481 ± 0.082
15.214AlaLeu: 15.214 ± 0.248
3.524AlaMet: 3.524 ± 0.058
2.828AlaAsn: 2.828 ± 0.067
6.299AlaPro: 6.299 ± 0.108
6.924AlaGln: 6.924 ± 0.125
8.768AlaArg: 8.768 ± 0.124
7.068AlaSer: 7.068 ± 0.101
6.174AlaThr: 6.174 ± 0.088
8.718AlaVal: 8.718 ± 0.092
2.303AlaTrp: 2.303 ± 0.055
2.286AlaTyr: 2.286 ± 0.046
0.0AlaXaa: 0.0 ± 0.0
Cys
1.07CysAla: 1.07 ± 0.03
0.12CysCys: 0.12 ± 0.009
0.486CysAsp: 0.486 ± 0.02
0.494CysGlu: 0.494 ± 0.02
0.292CysPhe: 0.292 ± 0.016
0.92CysGly: 0.92 ± 0.034
0.283CysHis: 0.283 ± 0.016
0.344CysIle: 0.344 ± 0.018
0.241CysLys: 0.241 ± 0.015
0.957CysLeu: 0.957 ± 0.032
0.216CysMet: 0.216 ± 0.012
0.225CysAsn: 0.225 ± 0.013
0.532CysPro: 0.532 ± 0.025
0.359CysGln: 0.359 ± 0.018
0.581CysArg: 0.581 ± 0.024
0.522CysSer: 0.522 ± 0.022
0.502CysThr: 0.502 ± 0.022
0.73CysVal: 0.73 ± 0.028
0.161CysTrp: 0.161 ± 0.012
0.179CysTyr: 0.179 ± 0.014
0.0CysXaa: 0.0 ± 0.0
Asp
6.565AspAla: 6.565 ± 0.087
0.436AspCys: 0.436 ± 0.021
2.847AspAsp: 2.847 ± 0.059
3.146AspGlu: 3.146 ± 0.063
1.809AspPhe: 1.809 ± 0.042
4.447AspGly: 4.447 ± 0.09
1.291AspHis: 1.291 ± 0.038
2.34AspIle: 2.34 ± 0.044
1.72AspLys: 1.72 ± 0.046
5.493AspLeu: 5.493 ± 0.081
1.22AspMet: 1.22 ± 0.037
1.284AspAsn: 1.284 ± 0.039
2.823AspPro: 2.823 ± 0.051
2.045AspGln: 2.045 ± 0.052
3.171AspArg: 3.171 ± 0.054
2.279AspSer: 2.279 ± 0.055
3.293AspThr: 3.293 ± 0.096
4.115AspVal: 4.115 ± 0.06
0.925AspTrp: 0.925 ± 0.028
1.092AspTyr: 1.092 ± 0.033
0.0AspXaa: 0.0 ± 0.0
Glu
7.213GluAla: 7.213 ± 0.101
0.382GluCys: 0.382 ± 0.019
2.006GluAsp: 2.006 ± 0.045
2.263GluGlu: 2.263 ± 0.053
1.684GluPhe: 1.684 ± 0.046
4.212GluGly: 4.212 ± 0.068
1.434GluHis: 1.434 ± 0.042
2.192GluIle: 2.192 ± 0.048
1.603GluLys: 1.603 ± 0.043
5.313GluLeu: 5.313 ± 0.075
1.143GluMet: 1.143 ± 0.032
1.032GluAsn: 1.032 ± 0.036
2.585GluPro: 2.585 ± 0.057
2.902GluGln: 2.902 ± 0.048
4.228GluArg: 4.228 ± 0.068
2.263GluSer: 2.263 ± 0.048
2.285GluThr: 2.285 ± 0.051
4.321GluVal: 4.321 ± 0.072
0.692GluTrp: 0.692 ± 0.027
0.852GluTyr: 0.852 ± 0.031
0.0GluXaa: 0.0 ± 0.0
Phe
3.462PheAla: 3.462 ± 0.064
0.358PheCys: 0.358 ± 0.02
2.375PheAsp: 2.375 ± 0.045
1.988PheGlu: 1.988 ± 0.048
1.206PhePhe: 1.206 ± 0.033
2.934PheGly: 2.934 ± 0.048
0.718PheHis: 0.718 ± 0.026
1.28PheIle: 1.28 ± 0.032
1.338PheLys: 1.338 ± 0.037
2.72PheLeu: 2.72 ± 0.055
0.849PheMet: 0.849 ± 0.029
1.177PheAsn: 1.177 ± 0.033
1.35PhePro: 1.35 ± 0.035
1.233PheGln: 1.233 ± 0.034
1.749PheArg: 1.749 ± 0.039
1.986PheSer: 1.986 ± 0.045
1.952PheThr: 1.952 ± 0.044
2.471PheVal: 2.471 ± 0.056
0.505PheTrp: 0.505 ± 0.023
0.803PheTyr: 0.803 ± 0.03
0.0PheXaa: 0.0 ± 0.0
Gly
8.612GlyAla: 8.612 ± 0.117
0.936GlyCys: 0.936 ± 0.033
3.957GlyAsp: 3.957 ± 0.084
4.049GlyGlu: 4.049 ± 0.068
2.995GlyPhe: 2.995 ± 0.056
6.849GlyGly: 6.849 ± 0.136
2.307GlyHis: 2.307 ± 0.049
3.305GlyIle: 3.305 ± 0.056
3.124GlyLys: 3.124 ± 0.065
9.723GlyLeu: 9.723 ± 0.105
2.3GlyMet: 2.3 ± 0.044
2.2GlyAsn: 2.2 ± 0.092
3.199GlyPro: 3.199 ± 0.057
4.578GlyGln: 4.578 ± 0.088
5.156GlyArg: 5.156 ± 0.07
4.545GlySer: 4.545 ± 0.098
4.604GlyThr: 4.604 ± 0.103
6.547GlyVal: 6.547 ± 0.082
1.594GlyTrp: 1.594 ± 0.041
1.908GlyTyr: 1.908 ± 0.045
0.0GlyXaa: 0.0 ± 0.0
His
3.016HisAla: 3.016 ± 0.063
0.288HisCys: 0.288 ± 0.018
1.361HisAsp: 1.361 ± 0.038
1.355HisGlu: 1.355 ± 0.039
0.92HisPhe: 0.92 ± 0.032
2.24HisGly: 2.24 ± 0.056
0.83HisHis: 0.83 ± 0.033
0.887HisIle: 0.887 ± 0.031
0.628HisLys: 0.628 ± 0.026
2.738HisLeu: 2.738 ± 0.06
0.518HisMet: 0.518 ± 0.019
0.538HisAsn: 0.538 ± 0.026
1.768HisPro: 1.768 ± 0.045
1.013HisGln: 1.013 ± 0.029
1.699HisArg: 1.699 ± 0.045
1.173HisSer: 1.173 ± 0.032
1.452HisThr: 1.452 ± 0.042
1.742HisVal: 1.742 ± 0.041
0.579HisTrp: 0.579 ± 0.025
0.549HisTyr: 0.549 ± 0.021
0.0HisXaa: 0.0 ± 0.0
Ile
4.341IleAla: 4.341 ± 0.067
0.317IleCys: 0.317 ± 0.016
3.029IleAsp: 3.029 ± 0.056
2.927IleGlu: 2.927 ± 0.055
1.038IlePhe: 1.038 ± 0.035
3.48IleGly: 3.48 ± 0.056
0.878IleHis: 0.878 ± 0.027
1.302IleIle: 1.302 ± 0.04
1.516IleLys: 1.516 ± 0.044
2.796IleLeu: 2.796 ± 0.053
0.706IleMet: 0.706 ± 0.028
1.351IleAsn: 1.351 ± 0.039
1.637IlePro: 1.637 ± 0.041
1.486IleGln: 1.486 ± 0.041
2.343IleArg: 2.343 ± 0.045
2.238IleSer: 2.238 ± 0.052
2.579IleThr: 2.579 ± 0.057
2.891IleVal: 2.891 ± 0.05
0.395IleTrp: 0.395 ± 0.021
0.749IleTyr: 0.749 ± 0.03
0.0IleXaa: 0.0 ± 0.0
Lys
4.684LysAla: 4.684 ± 0.098
0.173LysCys: 0.173 ± 0.013
1.624LysAsp: 1.624 ± 0.038
1.466LysGlu: 1.466 ± 0.044
0.94LysPhe: 0.94 ± 0.035
2.776LysGly: 2.776 ± 0.064
0.699LysHis: 0.699 ± 0.026
1.244LysIle: 1.244 ± 0.042
1.275LysLys: 1.275 ± 0.05
3.185LysLeu: 3.185 ± 0.061
0.703LysMet: 0.703 ± 0.028
0.833LysAsn: 0.833 ± 0.031
2.025LysPro: 2.025 ± 0.052
1.325LysGln: 1.325 ± 0.034
1.98LysArg: 1.98 ± 0.048
1.548LysSer: 1.548 ± 0.044
1.673LysThr: 1.673 ± 0.047
2.831LysVal: 2.831 ± 0.065
0.288LysTrp: 0.288 ± 0.015
0.541LysTyr: 0.541 ± 0.023
0.0LysXaa: 0.0 ± 0.0
Leu
13.991LeuAla: 13.991 ± 0.152
1.062LeuCys: 1.062 ± 0.031
5.83LeuAsp: 5.83 ± 0.079
4.742LeuGlu: 4.742 ± 0.077
3.066LeuPhe: 3.066 ± 0.054
8.881LeuGly: 8.881 ± 0.109
2.563LeuHis: 2.563 ± 0.052
4.131LeuIle: 4.131 ± 0.068
3.966LeuLys: 3.966 ± 0.064
10.769LeuLeu: 10.769 ± 0.148
2.95LeuMet: 2.95 ± 0.058
3.181LeuAsn: 3.181 ± 0.05
6.444LeuPro: 6.444 ± 0.095
5.374LeuGln: 5.374 ± 0.085
7.27LeuArg: 7.27 ± 0.092
7.117LeuSer: 7.117 ± 0.121
6.052LeuThr: 6.052 ± 0.149
7.865LeuVal: 7.865 ± 0.086
1.57LeuTrp: 1.57 ± 0.041
1.952LeuTyr: 1.952 ± 0.045
0.0LeuXaa: 0.0 ± 0.0
Met
3.573MetAla: 3.573 ± 0.061
0.172MetCys: 0.172 ± 0.013
1.172MetAsp: 1.172 ± 0.031
0.947MetGlu: 0.947 ± 0.027
0.649MetPhe: 0.649 ± 0.027
2.137MetGly: 2.137 ± 0.044
0.595MetHis: 0.595 ± 0.024
0.838MetIle: 0.838 ± 0.029
0.921MetLys: 0.921 ± 0.032
2.543MetLeu: 2.543 ± 0.053
0.531MetMet: 0.531 ± 0.022
0.819MetAsn: 0.819 ± 0.026
1.646MetPro: 1.646 ± 0.042
1.065MetGln: 1.065 ± 0.032
1.655MetArg: 1.655 ± 0.044
1.764MetSer: 1.764 ± 0.039
1.377MetThr: 1.377 ± 0.037
1.854MetVal: 1.854 ± 0.043
0.239MetTrp: 0.239 ± 0.014
0.338MetTyr: 0.338 ± 0.018
0.0MetXaa: 0.0 ± 0.0
Asn
3.183AsnAla: 3.183 ± 0.064
0.227AsnCys: 0.227 ± 0.014
1.464AsnAsp: 1.464 ± 0.068
1.209AsnGlu: 1.209 ± 0.034
0.874AsnPhe: 0.874 ± 0.028
2.379AsnGly: 2.379 ± 0.071
0.572AsnHis: 0.572 ± 0.024
1.054AsnIle: 1.054 ± 0.03
0.825AsnLys: 0.825 ± 0.031
2.797AsnLeu: 2.797 ± 0.051
0.581AsnMet: 0.581 ± 0.023
0.783AsnAsn: 0.783 ± 0.037
1.859AsnPro: 1.859 ± 0.048
1.04AsnGln: 1.04 ± 0.03
1.605AsnArg: 1.605 ± 0.04
1.229AsnSer: 1.229 ± 0.041
1.589AsnThr: 1.589 ± 0.048
2.017AsnVal: 2.017 ± 0.056
0.428AsnTrp: 0.428 ± 0.019
0.581AsnTyr: 0.581 ± 0.024
0.0AsnXaa: 0.0 ± 0.0
Pro
6.881ProAla: 6.881 ± 0.109
0.421ProCys: 0.421 ± 0.02
3.379ProAsp: 3.379 ± 0.066
3.632ProGlu: 3.632 ± 0.065
1.623ProPhe: 1.623 ± 0.044
4.605ProGly: 4.605 ± 0.073
1.286ProHis: 1.286 ± 0.041
1.708ProIle: 1.708 ± 0.042
1.575ProLys: 1.575 ± 0.045
5.358ProLeu: 5.358 ± 0.094
1.452ProMet: 1.452 ± 0.032
1.336ProAsn: 1.336 ± 0.033
2.662ProPro: 2.662 ± 0.068
2.443ProGln: 2.443 ± 0.047
3.029ProArg: 3.029 ± 0.062
2.979ProSer: 2.979 ± 0.054
2.976ProThr: 2.976 ± 0.052
4.623ProVal: 4.623 ± 0.081
1.032ProTrp: 1.032 ± 0.032
0.981ProTyr: 0.981 ± 0.032
0.0ProXaa: 0.0 ± 0.0
Gln
8.148GlnAla: 8.148 ± 0.121
0.354GlnCys: 0.354 ± 0.017
1.835GlnAsp: 1.835 ± 0.04
1.767GlnGlu: 1.767 ± 0.044
1.327GlnPhe: 1.327 ± 0.032
4.383GlnGly: 4.383 ± 0.074
1.291GlnHis: 1.291 ± 0.039
1.832GlnIle: 1.832 ± 0.056
1.108GlnLys: 1.108 ± 0.032
4.861GlnLeu: 4.861 ± 0.077
1.051GlnMet: 1.051 ± 0.034
0.893GlnAsn: 0.893 ± 0.031
2.903GlnPro: 2.903 ± 0.063
2.757GlnGln: 2.757 ± 0.054
3.891GlnArg: 3.891 ± 0.074
2.168GlnSer: 2.168 ± 0.05
2.13GlnThr: 2.13 ± 0.045
4.241GlnVal: 4.241 ± 0.13
0.975GlnTrp: 0.975 ± 0.035
0.768GlnTyr: 0.768 ± 0.03
0.0GlnXaa: 0.0 ± 0.0
Arg
7.057ArgAla: 7.057 ± 0.1
0.58ArgCys: 0.58 ± 0.026
3.37ArgAsp: 3.37 ± 0.066
3.743ArgGlu: 3.743 ± 0.064
2.587ArgPhe: 2.587 ± 0.051
4.182ArgGly: 4.182 ± 0.069
2.099ArgHis: 2.099 ± 0.053
2.969ArgIle: 2.969 ± 0.056
2.09ArgLys: 2.09 ± 0.047
8.034ArgLeu: 8.034 ± 0.114
1.886ArgMet: 1.886 ± 0.045
1.663ArgAsn: 1.663 ± 0.038
3.305ArgPro: 3.305 ± 0.065
3.761ArgGln: 3.761 ± 0.065
4.54ArgArg: 4.54 ± 0.073
3.349ArgSer: 3.349 ± 0.058
3.437ArgThr: 3.437 ± 0.054
5.068ArgVal: 5.068 ± 0.066
1.45ArgTrp: 1.45 ± 0.045
1.582ArgTyr: 1.582 ± 0.041
0.0ArgXaa: 0.0 ± 0.0
Ser
6.781SerAla: 6.781 ± 0.103
0.454SerCys: 0.454 ± 0.019
2.629SerAsp: 2.629 ± 0.056
2.462SerGlu: 2.462 ± 0.047
1.922SerPhe: 1.922 ± 0.042
5.04SerGly: 5.04 ± 0.101
1.27SerHis: 1.27 ± 0.039
2.104SerIle: 2.104 ± 0.049
1.575SerLys: 1.575 ± 0.041
6.207SerLeu: 6.207 ± 0.094
1.299SerMet: 1.299 ± 0.038
1.463SerAsn: 1.463 ± 0.044
3.171SerPro: 3.171 ± 0.059
2.318SerGln: 2.318 ± 0.05
3.588SerArg: 3.588 ± 0.06
3.181SerSer: 3.181 ± 0.083
3.371SerThr: 3.371 ± 0.111
4.252SerVal: 4.252 ± 0.079
0.816SerTrp: 0.816 ± 0.027
1.069SerTyr: 1.069 ± 0.033
0.0SerXaa: 0.0 ± 0.0
Thr
6.369ThrAla: 6.369 ± 0.182
0.429ThrCys: 0.429 ± 0.019
2.853ThrAsp: 2.853 ± 0.075
2.624ThrGlu: 2.624 ± 0.053
1.805ThrPhe: 1.805 ± 0.045
4.746ThrGly: 4.746 ± 0.096
1.263ThrHis: 1.263 ± 0.035
1.863ThrIle: 1.863 ± 0.053
1.14ThrLys: 1.14 ± 0.037
6.871ThrLeu: 6.871 ± 0.11
1.015ThrMet: 1.015 ± 0.031
1.282ThrAsn: 1.282 ± 0.046
3.783ThrPro: 3.783 ± 0.066
2.349ThrGln: 2.349 ± 0.07
3.421ThrArg: 3.421 ± 0.053
2.971ThrSer: 2.971 ± 0.072
3.152ThrThr: 3.152 ± 0.145
4.627ThrVal: 4.627 ± 0.106
0.843ThrTrp: 0.843 ± 0.025
1.138ThrTyr: 1.138 ± 0.042
0.0ThrXaa: 0.0 ± 0.0
Val
9.632ValAla: 9.632 ± 0.123
0.8ValCys: 0.8 ± 0.026
4.01ValAsp: 4.01 ± 0.068
3.692ValGlu: 3.692 ± 0.054
2.574ValPhe: 2.574 ± 0.045
5.857ValGly: 5.857 ± 0.084
1.93ValHis: 1.93 ± 0.048
3.214ValIle: 3.214 ± 0.064
2.605ValLys: 2.605 ± 0.062
8.651ValLeu: 8.651 ± 0.102
1.989ValMet: 1.989 ± 0.041
2.396ValAsn: 2.396 ± 0.058
4.118ValPro: 4.118 ± 0.073
3.74ValGln: 3.74 ± 0.071
5.127ValArg: 5.127 ± 0.061
4.641ValSer: 4.641 ± 0.092
4.085ValThr: 4.085 ± 0.097
6.829ValVal: 6.829 ± 0.093
1.213ValTrp: 1.213 ± 0.04
1.422ValTyr: 1.422 ± 0.035
0.0ValXaa: 0.0 ± 0.0
Trp
1.882TrpAla: 1.882 ± 0.053
0.215TrpCys: 0.215 ± 0.014
0.592TrpAsp: 0.592 ± 0.021
0.467TrpGlu: 0.467 ± 0.018
0.567TrpPhe: 0.567 ± 0.022
1.16TrpGly: 1.16 ± 0.031
0.463TrpHis: 0.463 ± 0.022
0.489TrpIle: 0.489 ± 0.021
0.438TrpLys: 0.438 ± 0.02
2.448TrpLeu: 2.448 ± 0.063
0.51TrpMet: 0.51 ± 0.021
0.359TrpAsn: 0.359 ± 0.019
0.973TrpPro: 0.973 ± 0.036
1.098TrpGln: 1.098 ± 0.034
1.516TrpArg: 1.516 ± 0.049
0.841TrpSer: 0.841 ± 0.027
0.669TrpThr: 0.669 ± 0.022
1.363TrpVal: 1.363 ± 0.034
0.419TrpTrp: 0.419 ± 0.022
0.275TrpTyr: 0.275 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.148TyrAla: 2.148 ± 0.044
0.185TyrCys: 0.185 ± 0.013
1.092TyrAsp: 1.092 ± 0.035
1.02TyrGlu: 1.02 ± 0.032
0.816TyrPhe: 0.816 ± 0.026
1.64TyrGly: 1.64 ± 0.039
0.437TyrHis: 0.437 ± 0.019
0.689TyrIle: 0.689 ± 0.029
0.646TyrLys: 0.646 ± 0.024
2.101TyrLeu: 2.101 ± 0.043
0.394TyrMet: 0.394 ± 0.018
0.654TyrAsn: 0.654 ± 0.024
0.899TyrPro: 0.899 ± 0.029
0.871TyrGln: 0.871 ± 0.031
1.435TyrArg: 1.435 ± 0.037
1.11TyrSer: 1.11 ± 0.034
1.193TyrThr: 1.193 ± 0.037
1.418TyrVal: 1.418 ± 0.038
0.318TyrTrp: 0.318 ± 0.016
0.438TyrTyr: 0.438 ± 0.022
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3324 proteins (1183161 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski