Barnesiella sp. WM24

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Barnesiellaceae; Barnesiella; unclassified Barnesiella

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3000 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y9IYA2|A0A4Y9IYA2_9BACT Uncharacterized protein OS=Barnesiella sp. WM24 OX=2558278 GN=E4T81_06310 PE=4 SV=1
MM1 pKa = 7.32SFWNILGEE9 pKa = 3.9LAIFNMIRR17 pKa = 11.84NLLSQKK23 pKa = 10.19KK24 pKa = 8.71KK25 pKa = 9.62QQSLPYY31 pKa = 8.65PHH33 pKa = 6.99SKK35 pKa = 10.54VPDD38 pKa = 3.92HH39 pKa = 7.45DD40 pKa = 4.0PMRR43 pKa = 11.84EE44 pKa = 3.8ARR46 pKa = 11.84MEE48 pKa = 4.0QLDD51 pKa = 3.97NEE53 pKa = 4.47YY54 pKa = 11.14VIASKK59 pKa = 9.94PAAGRR64 pKa = 11.84YY65 pKa = 6.46EE66 pKa = 4.06TTEE69 pKa = 4.18YY70 pKa = 10.69IPLNDD75 pKa = 3.72TDD77 pKa = 5.63DD78 pKa = 4.31LQDD81 pKa = 3.92YY82 pKa = 7.85DD83 pKa = 4.93ACDD86 pKa = 3.57LQDD89 pKa = 4.8RR90 pKa = 11.84INDD93 pKa = 4.1LEE95 pKa = 4.26SQLDD99 pKa = 3.66DD100 pKa = 3.86CDD102 pKa = 4.51YY103 pKa = 10.69EE104 pKa = 4.54SEE106 pKa = 4.61YY107 pKa = 10.87YY108 pKa = 10.83DD109 pKa = 5.08HH110 pKa = 7.03IQDD113 pKa = 4.89EE114 pKa = 4.63IDD116 pKa = 3.47MLQDD120 pKa = 3.26QLDD123 pKa = 4.34EE124 pKa = 5.65IEE126 pKa = 4.67DD127 pKa = 3.58LRR129 pKa = 11.84NIYY132 pKa = 10.53DD133 pKa = 4.72DD134 pKa = 4.5EE135 pKa = 6.85LDD137 pKa = 4.93DD138 pKa = 6.4EE139 pKa = 4.82LDD141 pKa = 3.54EE142 pKa = 5.09WDD144 pKa = 5.24DD145 pKa = 4.22LQDD148 pKa = 5.47DD149 pKa = 4.93EE150 pKa = 6.43IDD152 pKa = 3.95DD153 pKa = 4.97FDD155 pKa = 4.36YY156 pKa = 11.5QDD158 pKa = 4.19LGLYY162 pKa = 10.42DD163 pKa = 3.31DD164 pKa = 4.82TYY166 pKa = 11.46RR167 pKa = 11.84DD168 pKa = 3.58EE169 pKa = 4.88EE170 pKa = 4.29DD171 pKa = 2.91WW172 pKa = 4.85

Molecular weight: 20.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y9IVS7|A0A4Y9IVS7_9BACT Lysine exporter LysO family protein OS=Barnesiella sp. WM24 OX=2558278 GN=E4T81_11360 PE=4 SV=1
MM1 pKa = 7.49TDD3 pKa = 2.66VMTPEE8 pKa = 3.93QRR10 pKa = 11.84HH11 pKa = 5.18KK12 pKa = 11.18CMAAIKK18 pKa = 10.63GRR20 pKa = 11.84DD21 pKa = 3.65TKK23 pKa = 11.07PEE25 pKa = 4.64LIVRR29 pKa = 11.84RR30 pKa = 11.84FLHH33 pKa = 6.52ARR35 pKa = 11.84GLRR38 pKa = 11.84YY39 pKa = 9.73RR40 pKa = 11.84LNNRR44 pKa = 11.84NLPGTPDD51 pKa = 3.15IVLKK55 pKa = 10.51KK56 pKa = 9.17YY57 pKa = 9.34RR58 pKa = 11.84TVIFIDD64 pKa = 3.67GCFWHH69 pKa = 6.54GHH71 pKa = 4.76EE72 pKa = 5.08GCRR75 pKa = 11.84QFKK78 pKa = 10.34LPKK81 pKa = 10.24SNTDD85 pKa = 3.68FWRR88 pKa = 11.84HH89 pKa = 5.58KK90 pKa = 10.47INMNIARR97 pKa = 11.84DD98 pKa = 3.55YY99 pKa = 10.09RR100 pKa = 11.84ANVEE104 pKa = 3.94LRR106 pKa = 11.84LLGWRR111 pKa = 11.84VIRR114 pKa = 11.84VWEE117 pKa = 4.22CEE119 pKa = 3.51IRR121 pKa = 11.84PQSTASTRR129 pKa = 11.84LEE131 pKa = 3.83SLYY134 pKa = 10.8RR135 pKa = 11.84SS136 pKa = 3.75

Molecular weight: 16.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3000

0

3000

1038617

34

2729

346.2

38.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.959 ± 0.046

1.24 ± 0.016

6.303 ± 0.038

6.165 ± 0.039

4.207 ± 0.028

7.069 ± 0.04

1.872 ± 0.017

6.966 ± 0.04

5.665 ± 0.04

8.64 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.752 ± 0.018

4.819 ± 0.031

3.961 ± 0.027

2.729 ± 0.027

5.143 ± 0.038

6.514 ± 0.037

5.709 ± 0.034

6.861 ± 0.035

1.249 ± 0.015

4.178 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski