Amino acid dipepetide frequency for Barnesiella sp. WM24

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.394AlaAla: 7.394 ± 0.106
0.968AlaCys: 0.968 ± 0.029
5.122AlaAsp: 5.122 ± 0.072
5.083AlaGlu: 5.083 ± 0.08
3.012AlaPhe: 3.012 ± 0.062
5.893AlaGly: 5.893 ± 0.087
1.259AlaHis: 1.259 ± 0.035
5.704AlaIle: 5.704 ± 0.072
4.194AlaLys: 4.194 ± 0.081
7.153AlaLeu: 7.153 ± 0.096
2.362AlaMet: 2.362 ± 0.05
3.073AlaAsn: 3.073 ± 0.061
2.833AlaPro: 2.833 ± 0.061
2.442AlaGln: 2.442 ± 0.057
3.724AlaArg: 3.724 ± 0.061
4.893AlaSer: 4.893 ± 0.08
4.883AlaThr: 4.883 ± 0.082
5.744AlaVal: 5.744 ± 0.093
0.912AlaTrp: 0.912 ± 0.029
2.94AlaTyr: 2.94 ± 0.064
0.0AlaXaa: 0.0 ± 0.0
Cys
0.875CysAla: 0.875 ± 0.029
0.206CysCys: 0.206 ± 0.013
0.85CysAsp: 0.85 ± 0.037
0.655CysGlu: 0.655 ± 0.024
0.552CysPhe: 0.552 ± 0.025
1.089CysGly: 1.089 ± 0.033
0.369CysHis: 0.369 ± 0.019
0.777CysIle: 0.777 ± 0.03
0.588CysLys: 0.588 ± 0.024
0.984CysLeu: 0.984 ± 0.035
0.281CysMet: 0.281 ± 0.018
0.61CysAsn: 0.61 ± 0.023
0.52CysPro: 0.52 ± 0.021
0.266CysGln: 0.266 ± 0.015
0.803CysArg: 0.803 ± 0.035
0.866CysSer: 0.866 ± 0.027
0.596CysThr: 0.596 ± 0.026
0.824CysVal: 0.824 ± 0.029
0.159CysTrp: 0.159 ± 0.012
0.535CysTyr: 0.535 ± 0.024
0.0CysXaa: 0.0 ± 0.0
Asp
4.796AspAla: 4.796 ± 0.073
0.814AspCys: 0.814 ± 0.031
3.954AspAsp: 3.954 ± 0.068
4.082AspGlu: 4.082 ± 0.061
3.187AspPhe: 3.187 ± 0.054
5.038AspGly: 5.038 ± 0.1
0.913AspHis: 0.913 ± 0.031
4.754AspIle: 4.754 ± 0.071
3.556AspLys: 3.556 ± 0.055
4.578AspLeu: 4.578 ± 0.07
1.881AspMet: 1.881 ± 0.048
3.348AspAsn: 3.348 ± 0.067
2.271AspPro: 2.271 ± 0.057
1.056AspGln: 1.056 ± 0.032
3.309AspArg: 3.309 ± 0.054
4.161AspSer: 4.161 ± 0.068
3.235AspThr: 3.235 ± 0.061
4.116AspVal: 4.116 ± 0.073
0.869AspTrp: 0.869 ± 0.027
3.109AspTyr: 3.109 ± 0.057
0.0AspXaa: 0.0 ± 0.0
Glu
5.005GluAla: 5.005 ± 0.087
0.68GluCys: 0.68 ± 0.028
2.838GluAsp: 2.838 ± 0.056
4.329GluGlu: 4.329 ± 0.08
2.384GluPhe: 2.384 ± 0.051
3.94GluGly: 3.94 ± 0.07
1.082GluHis: 1.082 ± 0.034
4.767GluIle: 4.767 ± 0.069
4.267GluLys: 4.267 ± 0.072
5.711GluLeu: 5.711 ± 0.084
1.894GluMet: 1.894 ± 0.044
3.127GluAsn: 3.127 ± 0.06
2.029GluPro: 2.029 ± 0.049
2.058GluGln: 2.058 ± 0.046
3.422GluArg: 3.422 ± 0.06
3.572GluSer: 3.572 ± 0.059
3.089GluThr: 3.089 ± 0.057
3.98GluVal: 3.98 ± 0.065
0.846GluTrp: 0.846 ± 0.028
2.634GluTyr: 2.634 ± 0.051
0.0GluXaa: 0.0 ± 0.0
Phe
3.186PheAla: 3.186 ± 0.057
0.621PheCys: 0.621 ± 0.026
2.959PheAsp: 2.959 ± 0.058
2.144PheGlu: 2.144 ± 0.043
1.874PhePhe: 1.874 ± 0.047
3.2PheGly: 3.2 ± 0.071
0.798PheHis: 0.798 ± 0.029
2.966PheIle: 2.966 ± 0.067
2.137PheLys: 2.137 ± 0.042
3.276PheLeu: 3.276 ± 0.062
1.196PheMet: 1.196 ± 0.037
2.381PheAsn: 2.381 ± 0.055
1.674PhePro: 1.674 ± 0.037
0.92PheGln: 0.92 ± 0.03
2.081PheArg: 2.081 ± 0.043
3.089PheSer: 3.089 ± 0.06
2.735PheThr: 2.735 ± 0.049
2.578PheVal: 2.578 ± 0.053
0.533PheTrp: 0.533 ± 0.024
1.716PheTyr: 1.716 ± 0.039
0.0PheXaa: 0.0 ± 0.0
Gly
5.192GlyAla: 5.192 ± 0.081
1.132GlyCys: 1.132 ± 0.039
4.416GlyAsp: 4.416 ± 0.074
4.347GlyGlu: 4.347 ± 0.076
3.072GlyPhe: 3.072 ± 0.059
4.945GlyGly: 4.945 ± 0.086
1.375GlyHis: 1.375 ± 0.037
5.322GlyIle: 5.322 ± 0.079
4.915GlyLys: 4.915 ± 0.066
5.66GlyLeu: 5.66 ± 0.083
2.098GlyMet: 2.098 ± 0.053
3.664GlyAsn: 3.664 ± 0.071
1.282GlyPro: 1.282 ± 0.04
1.743GlyGln: 1.743 ± 0.047
3.236GlyArg: 3.236 ± 0.067
4.441GlySer: 4.441 ± 0.07
4.025GlyThr: 4.025 ± 0.073
5.373GlyVal: 5.373 ± 0.079
1.06GlyTrp: 1.06 ± 0.036
3.389GlyTyr: 3.389 ± 0.058
0.0GlyXaa: 0.0 ± 0.0
His
1.274HisAla: 1.274 ± 0.039
0.283HisCys: 0.283 ± 0.017
1.283HisAsp: 1.283 ± 0.035
1.082HisGlu: 1.082 ± 0.032
0.89HisPhe: 0.89 ± 0.031
1.397HisGly: 1.397 ± 0.039
0.509HisHis: 0.509 ± 0.027
1.397HisIle: 1.397 ± 0.035
0.938HisLys: 0.938 ± 0.032
1.594HisLeu: 1.594 ± 0.043
0.385HisMet: 0.385 ± 0.018
0.957HisAsn: 0.957 ± 0.031
1.031HisPro: 1.031 ± 0.031
0.447HisGln: 0.447 ± 0.019
0.971HisArg: 0.971 ± 0.032
1.192HisSer: 1.192 ± 0.035
0.967HisThr: 0.967 ± 0.03
1.091HisVal: 1.091 ± 0.036
0.23HisTrp: 0.23 ± 0.014
0.803HisTyr: 0.803 ± 0.028
0.0HisXaa: 0.0 ± 0.0
Ile
6.184IleAla: 6.184 ± 0.089
0.92IleCys: 0.92 ± 0.03
5.119IleAsp: 5.119 ± 0.066
4.424IleGlu: 4.424 ± 0.068
2.645IlePhe: 2.645 ± 0.056
4.764IleGly: 4.764 ± 0.084
1.234IleHis: 1.234 ± 0.037
4.884IleIle: 4.884 ± 0.078
3.996IleLys: 3.996 ± 0.059
5.34IleLeu: 5.34 ± 0.081
1.666IleMet: 1.666 ± 0.04
3.614IleAsn: 3.614 ± 0.064
3.135IlePro: 3.135 ± 0.06
1.586IleGln: 1.586 ± 0.042
2.97IleArg: 2.97 ± 0.056
4.81IleSer: 4.81 ± 0.079
4.209IleThr: 4.209 ± 0.064
4.882IleVal: 4.882 ± 0.074
0.666IleTrp: 0.666 ± 0.024
2.607IleTyr: 2.607 ± 0.048
0.0IleXaa: 0.0 ± 0.0
Lys
4.607LysAla: 4.607 ± 0.076
0.538LysCys: 0.538 ± 0.026
3.19LysAsp: 3.19 ± 0.06
4.309LysGlu: 4.309 ± 0.078
2.002LysPhe: 2.002 ± 0.045
3.808LysGly: 3.808 ± 0.065
1.002LysHis: 1.002 ± 0.03
3.822LysIle: 3.822 ± 0.065
3.713LysLys: 3.713 ± 0.068
4.555LysLeu: 4.555 ± 0.059
1.82LysMet: 1.82 ± 0.039
2.63LysAsn: 2.63 ± 0.052
2.166LysPro: 2.166 ± 0.049
1.648LysGln: 1.648 ± 0.048
2.942LysArg: 2.942 ± 0.052
3.671LysSer: 3.671 ± 0.057
3.029LysThr: 3.029 ± 0.064
4.065LysVal: 4.065 ± 0.073
0.727LysTrp: 0.727 ± 0.025
2.401LysTyr: 2.401 ± 0.053
0.0LysXaa: 0.0 ± 0.0
Leu
6.83LeuAla: 6.83 ± 0.1
1.233LeuCys: 1.233 ± 0.038
5.326LeuAsp: 5.326 ± 0.081
4.409LeuGlu: 4.409 ± 0.067
3.597LeuPhe: 3.597 ± 0.066
5.619LeuGly: 5.619 ± 0.076
1.721LeuHis: 1.721 ± 0.044
5.328LeuIle: 5.328 ± 0.081
4.87LeuLys: 4.87 ± 0.082
7.833LeuLeu: 7.833 ± 0.105
2.392LeuMet: 2.392 ± 0.054
4.17LeuAsn: 4.17 ± 0.057
4.212LeuPro: 4.212 ± 0.063
2.489LeuGln: 2.489 ± 0.055
4.782LeuArg: 4.782 ± 0.077
6.71LeuSer: 6.71 ± 0.099
5.369LeuThr: 5.369 ± 0.077
5.158LeuVal: 5.158 ± 0.078
0.97LeuTrp: 0.97 ± 0.029
3.379LeuTyr: 3.379 ± 0.059
0.0LeuXaa: 0.0 ± 0.0
Met
2.158MetAla: 2.158 ± 0.048
0.264MetCys: 0.264 ± 0.017
1.303MetAsp: 1.303 ± 0.035
1.674MetGlu: 1.674 ± 0.039
1.064MetPhe: 1.064 ± 0.034
1.67MetGly: 1.67 ± 0.044
0.471MetHis: 0.471 ± 0.023
1.682MetIle: 1.682 ± 0.043
2.152MetLys: 2.152 ± 0.043
2.637MetLeu: 2.637 ± 0.051
0.88MetMet: 0.88 ± 0.031
1.356MetAsn: 1.356 ± 0.036
1.355MetPro: 1.355 ± 0.035
0.868MetGln: 0.868 ± 0.033
1.487MetArg: 1.487 ± 0.04
1.924MetSer: 1.924 ± 0.044
1.953MetThr: 1.953 ± 0.043
1.526MetVal: 1.526 ± 0.04
0.312MetTrp: 0.312 ± 0.02
0.784MetTyr: 0.784 ± 0.029
0.0MetXaa: 0.0 ± 0.0
Asn
3.622AsnAla: 3.622 ± 0.073
0.55AsnCys: 0.55 ± 0.025
2.922AsnAsp: 2.922 ± 0.057
2.658AsnGlu: 2.658 ± 0.059
1.902AsnPhe: 1.902 ± 0.053
3.844AsnGly: 3.844 ± 0.078
1.007AsnHis: 1.007 ± 0.032
3.557AsnIle: 3.557 ± 0.064
2.497AsnLys: 2.497 ± 0.049
4.112AsnLeu: 4.112 ± 0.064
1.177AsnMet: 1.177 ± 0.035
2.44AsnAsn: 2.44 ± 0.059
2.687AsnPro: 2.687 ± 0.052
1.24AsnGln: 1.24 ± 0.038
2.647AsnArg: 2.647 ± 0.058
2.886AsnSer: 2.886 ± 0.057
2.485AsnThr: 2.485 ± 0.044
3.313AsnVal: 3.313 ± 0.06
0.605AsnTrp: 0.605 ± 0.026
2.042AsnTyr: 2.042 ± 0.046
0.0AsnXaa: 0.0 ± 0.0
Pro
3.506ProAla: 3.506 ± 0.072
0.416ProCys: 0.416 ± 0.019
3.321ProAsp: 3.321 ± 0.067
3.467ProGlu: 3.467 ± 0.058
1.647ProPhe: 1.647 ± 0.038
2.957ProGly: 2.957 ± 0.061
0.724ProHis: 0.724 ± 0.027
2.085ProIle: 2.085 ± 0.042
1.755ProLys: 1.755 ± 0.049
3.206ProLeu: 3.206 ± 0.053
0.98ProMet: 0.98 ± 0.032
1.32ProAsn: 1.32 ± 0.039
0.976ProPro: 0.976 ± 0.036
1.241ProGln: 1.241 ± 0.031
1.669ProArg: 1.669 ± 0.042
2.534ProSer: 2.534 ± 0.059
1.955ProThr: 1.955 ± 0.047
3.653ProVal: 3.653 ± 0.067
0.486ProTrp: 0.486 ± 0.02
1.709ProTyr: 1.709 ± 0.035
0.0ProXaa: 0.0 ± 0.0
Gln
2.148GlnAla: 2.148 ± 0.048
0.3GlnCys: 0.3 ± 0.015
1.189GlnAsp: 1.189 ± 0.035
1.715GlnGlu: 1.715 ± 0.047
1.227GlnPhe: 1.227 ± 0.035
1.66GlnGly: 1.66 ± 0.046
0.505GlnHis: 0.505 ± 0.02
1.78GlnIle: 1.78 ± 0.043
1.612GlnLys: 1.612 ± 0.045
2.698GlnLeu: 2.698 ± 0.059
0.87GlnMet: 0.87 ± 0.032
1.244GlnAsn: 1.244 ± 0.045
1.159GlnPro: 1.159 ± 0.032
1.11GlnGln: 1.11 ± 0.049
1.567GlnArg: 1.567 ± 0.039
1.843GlnSer: 1.843 ± 0.047
1.393GlnThr: 1.393 ± 0.038
1.587GlnVal: 1.587 ± 0.044
0.425GlnTrp: 0.425 ± 0.021
1.255GlnTyr: 1.255 ± 0.037
0.0GlnXaa: 0.0 ± 0.0
Arg
3.269ArgAla: 3.269 ± 0.057
0.589ArgCys: 0.589 ± 0.024
3.01ArgAsp: 3.01 ± 0.059
3.255ArgGlu: 3.255 ± 0.064
2.426ArgPhe: 2.426 ± 0.047
2.869ArgGly: 2.869 ± 0.062
1.412ArgHis: 1.412 ± 0.04
3.692ArgIle: 3.692 ± 0.064
3.106ArgLys: 3.106 ± 0.053
5.336ArgLeu: 5.336 ± 0.077
1.486ArgMet: 1.486 ± 0.04
2.629ArgAsn: 2.629 ± 0.047
1.809ArgPro: 1.809 ± 0.051
1.857ArgGln: 1.857 ± 0.047
3.341ArgArg: 3.341 ± 0.069
2.656ArgSer: 2.656 ± 0.05
2.294ArgThr: 2.294 ± 0.048
3.172ArgVal: 3.172 ± 0.057
0.729ArgTrp: 0.729 ± 0.026
2.486ArgTyr: 2.486 ± 0.051
0.0ArgXaa: 0.0 ± 0.0
Ser
5.001SerAla: 5.001 ± 0.079
0.792SerCys: 0.792 ± 0.03
4.232SerAsp: 4.232 ± 0.07
3.592SerGlu: 3.592 ± 0.06
2.902SerPhe: 2.902 ± 0.053
5.114SerGly: 5.114 ± 0.081
1.256SerHis: 1.256 ± 0.038
4.457SerIle: 4.457 ± 0.077
3.011SerLys: 3.011 ± 0.054
6.28SerLeu: 6.28 ± 0.093
1.616SerMet: 1.616 ± 0.043
2.551SerAsn: 2.551 ± 0.054
2.704SerPro: 2.704 ± 0.053
1.919SerGln: 1.919 ± 0.042
3.671SerArg: 3.671 ± 0.064
4.178SerSer: 4.178 ± 0.067
3.649SerThr: 3.649 ± 0.069
4.716SerVal: 4.716 ± 0.077
0.822SerTrp: 0.822 ± 0.031
2.676SerTyr: 2.676 ± 0.058
0.0SerXaa: 0.0 ± 0.0
Thr
4.733ThrAla: 4.733 ± 0.077
0.532ThrCys: 0.532 ± 0.025
3.858ThrAsp: 3.858 ± 0.059
3.169ThrGlu: 3.169 ± 0.061
2.591ThrPhe: 2.591 ± 0.048
4.751ThrGly: 4.751 ± 0.076
0.969ThrHis: 0.969 ± 0.03
4.025ThrIle: 4.025 ± 0.076
2.35ThrLys: 2.35 ± 0.052
5.419ThrLeu: 5.419 ± 0.081
1.214ThrMet: 1.214 ± 0.037
2.139ThrAsn: 2.139 ± 0.051
3.148ThrPro: 3.148 ± 0.049
1.35ThrGln: 1.35 ± 0.037
2.555ThrArg: 2.555 ± 0.048
3.492ThrSer: 3.492 ± 0.066
3.193ThrThr: 3.193 ± 0.07
4.696ThrVal: 4.696 ± 0.084
0.633ThrTrp: 0.633 ± 0.026
2.274ThrTyr: 2.274 ± 0.053
0.001ThrXaa: 0.001 ± 0.001
Val
6.092ValAla: 6.092 ± 0.087
0.822ValCys: 0.822 ± 0.028
4.544ValAsp: 4.544 ± 0.075
4.228ValGlu: 4.228 ± 0.067
2.654ValPhe: 2.654 ± 0.058
4.168ValGly: 4.168 ± 0.071
0.982ValHis: 0.982 ± 0.033
4.996ValIle: 4.996 ± 0.085
4.306ValLys: 4.306 ± 0.059
5.282ValLeu: 5.282 ± 0.088
1.891ValMet: 1.891 ± 0.052
3.792ValAsn: 3.792 ± 0.071
2.625ValPro: 2.625 ± 0.055
1.421ValGln: 1.421 ± 0.039
3.128ValArg: 3.128 ± 0.053
4.666ValSer: 4.666 ± 0.076
4.88ValThr: 4.88 ± 0.103
4.806ValVal: 4.806 ± 0.076
0.753ValTrp: 0.753 ± 0.031
2.578ValTyr: 2.578 ± 0.051
0.0ValXaa: 0.0 ± 0.0
Trp
0.826TrpAla: 0.826 ± 0.029
0.165TrpCys: 0.165 ± 0.011
0.759TrpAsp: 0.759 ± 0.029
0.749TrpGlu: 0.749 ± 0.028
0.516TrpPhe: 0.516 ± 0.026
1.015TrpGly: 1.015 ± 0.038
0.306TrpHis: 0.306 ± 0.019
0.814TrpIle: 0.814 ± 0.031
0.621TrpLys: 0.621 ± 0.023
1.234TrpLeu: 1.234 ± 0.036
0.356TrpMet: 0.356 ± 0.02
0.68TrpAsn: 0.68 ± 0.028
0.224TrpPro: 0.224 ± 0.018
0.476TrpGln: 0.476 ± 0.026
0.755TrpArg: 0.755 ± 0.027
0.772TrpSer: 0.772 ± 0.027
0.728TrpThr: 0.728 ± 0.03
0.738TrpVal: 0.738 ± 0.03
0.243TrpTrp: 0.243 ± 0.016
0.517TrpTyr: 0.517 ± 0.022
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.89TyrAla: 2.89 ± 0.058
0.579TyrCys: 0.579 ± 0.03
2.834TyrAsp: 2.834 ± 0.05
2.283TyrGlu: 2.283 ± 0.046
1.926TyrPhe: 1.926 ± 0.043
2.947TyrGly: 2.947 ± 0.057
0.825TyrHis: 0.825 ± 0.026
2.847TyrIle: 2.847 ± 0.052
2.063TyrLys: 2.063 ± 0.045
3.507TyrLeu: 3.507 ± 0.065
1.073TyrMet: 1.073 ± 0.029
2.466TyrAsn: 2.466 ± 0.054
1.768TyrPro: 1.768 ± 0.043
1.151TyrGln: 1.151 ± 0.037
2.367TyrArg: 2.367 ± 0.054
2.782TyrSer: 2.782 ± 0.055
2.423TyrThr: 2.423 ± 0.055
2.593TyrVal: 2.593 ± 0.054
0.513TyrTrp: 0.513 ± 0.024
1.949TyrTyr: 1.949 ± 0.053
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.001XaaThr: 0.001 ± 0.001
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3000 proteins (1038617 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski