Pseudomonas phage pf8_ST274-AUS411

Taxonomy: Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes; Tubulavirales; Inoviridae; unclassified Inoviridae

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 16 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9J4B2|A0A6B9J4B2_9VIRU Uncharacterized protein OS=Pseudomonas phage pf8_ST274-AUS411 OX=2686285 PE=4 SV=1
MM1 pKa = 7.79RR2 pKa = 11.84IKK4 pKa = 10.19RR5 pKa = 11.84TLLVLLTLFMSVCASAEE22 pKa = 3.87DD23 pKa = 4.78YY24 pKa = 10.1YY25 pKa = 10.62WPRR28 pKa = 11.84GTQKK32 pKa = 9.82YY33 pKa = 8.53GSYY36 pKa = 9.51MEE38 pKa = 4.29VVEE41 pKa = 4.74EE42 pKa = 3.89ARR44 pKa = 11.84KK45 pKa = 9.19AAIANNPGYY54 pKa = 10.76SRR56 pKa = 11.84VEE58 pKa = 4.01AVRR61 pKa = 11.84VIYY64 pKa = 8.8PANGRR69 pKa = 11.84QDD71 pKa = 3.05MATYY75 pKa = 10.55GLEE78 pKa = 4.63FYY80 pKa = 10.71CLSQGVEE87 pKa = 4.42RR88 pKa = 11.84MCSTSYY94 pKa = 11.27NNPVYY99 pKa = 10.49RR100 pKa = 11.84KK101 pKa = 10.45GEE103 pKa = 3.98GCTAPKK109 pKa = 10.31IPDD112 pKa = 3.7EE113 pKa = 4.32TTGTCKK119 pKa = 10.35EE120 pKa = 4.38PPTPPEE126 pKa = 4.1DD127 pKa = 4.36CIKK130 pKa = 11.06GLTDD134 pKa = 4.65LFSSPPSNIFVSGGRR149 pKa = 11.84NFVNSSPPTGCKK161 pKa = 9.91NGCQYY166 pKa = 11.41LPTTSKK172 pKa = 7.66TTSCYY177 pKa = 10.13RR178 pKa = 11.84YY179 pKa = 9.48PGSDD183 pKa = 2.89NQGFCNYY190 pKa = 9.86VLMTDD195 pKa = 4.32GSACAADD202 pKa = 3.63SGNPGMTGPSLNDD215 pKa = 3.57TPPTNPDD222 pKa = 3.95EE223 pKa = 4.85PPSDD227 pKa = 4.41PNDD230 pKa = 4.17PGCPPGYY237 pKa = 8.96SWSGTTCVKK246 pKa = 10.27TPTDD250 pKa = 3.57PTEE253 pKa = 4.25PGGDD257 pKa = 3.65GGDD260 pKa = 3.91GGDD263 pKa = 4.12GGNTGGGDD271 pKa = 3.29GGGDD275 pKa = 2.96NGGGNDD281 pKa = 3.77NGGGDD286 pKa = 3.94GGTGGSDD293 pKa = 2.98GSGGNGEE300 pKa = 4.28GGGDD304 pKa = 3.57GSGGGDD310 pKa = 3.27GSGGGTGGGDD320 pKa = 3.45GGDD323 pKa = 3.81GGDD326 pKa = 5.45GGNCDD331 pKa = 4.17PAKK334 pKa = 10.16QGCSPGPAGPGGEE347 pKa = 4.21LKK349 pKa = 10.5EE350 pKa = 4.48PKK352 pKa = 9.97PGTWDD357 pKa = 3.36DD358 pKa = 6.11AIATWEE364 pKa = 4.22QKK366 pKa = 10.35VEE368 pKa = 4.01QAKK371 pKa = 10.49KK372 pKa = 8.89EE373 pKa = 4.12LKK375 pKa = 10.59DD376 pKa = 3.45KK377 pKa = 11.0VRR379 pKa = 11.84ANVDD383 pKa = 3.19QMKK386 pKa = 10.62GAFDD390 pKa = 4.66LNLAEE395 pKa = 5.82GGGQLPCEE403 pKa = 4.52SVTIWGRR410 pKa = 11.84SYY412 pKa = 11.07SLCVADD418 pKa = 5.85YY419 pKa = 11.46ADD421 pKa = 3.42QLSNLRR427 pKa = 11.84VALLLMAALIAAFILLRR444 pKa = 11.84DD445 pKa = 3.62

Molecular weight: 45.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9J9N2|A0A6B9J9N2_9VIRU Replication initiation protein OS=Pseudomonas phage pf8_ST274-AUS411 OX=2686285 PE=4 SV=1
MM1 pKa = 7.58LAKK4 pKa = 9.49TLKK7 pKa = 10.76ALLLLCLIQAARR19 pKa = 11.84TVADD23 pKa = 3.95PVKK26 pKa = 10.4GRR28 pKa = 11.84APGSSEE34 pKa = 3.48QLHH37 pKa = 6.03RR38 pKa = 11.84SGEE41 pKa = 4.27RR42 pKa = 11.84KK43 pKa = 9.53HH44 pKa = 6.5GRR46 pKa = 11.84SAPLNASPLKK56 pKa = 10.05QPPLGSVGQLLRR68 pKa = 11.84PALPSPRR75 pKa = 11.84RR76 pKa = 11.84QEE78 pKa = 4.28RR79 pKa = 11.84DD80 pKa = 3.23DD81 pKa = 3.5KK82 pKa = 11.56GRR84 pKa = 11.84ALGVALRR91 pKa = 11.84VPRR94 pKa = 11.84RR95 pKa = 11.84SVPLAVGDD103 pKa = 3.97DD104 pKa = 3.83VAIGTVTRR112 pKa = 11.84MGRR115 pKa = 11.84DD116 pKa = 3.43EE117 pKa = 5.0HH118 pKa = 7.03PWLAWFASEE127 pKa = 4.71

Molecular weight: 13.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

16

0

16

3003

30

512

187.7

20.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.724 ± 0.998

1.532 ± 0.418

5.661 ± 0.422

5.128 ± 0.405

3.63 ± 0.439

8.858 ± 1.695

2.198 ± 0.558

5.128 ± 0.738

4.862 ± 0.438

9.491 ± 0.916

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.331 ± 0.24

3.064 ± 0.545

6.327 ± 0.894

3.497 ± 0.297

6.593 ± 0.9

6.294 ± 0.581

5.295 ± 0.509

5.794 ± 0.355

1.732 ± 0.301

2.864 ± 0.317
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski