Prunus avium (Cherry) (Cerasus avium)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Rosales; Rosaceae;

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 30515 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6P5SZ50|A0A6P5SZ50_PRUAV 30S ribosomal protein 2 chloroplastic OS=Prunus avium OX=42229 GN=LOC110760947 PE=4 SV=1
MM1 pKa = 7.71PSSSSSSPNNYY12 pKa = 9.43NIKK15 pKa = 9.96ILASILFAILLILCAGAGYY34 pKa = 10.92GEE36 pKa = 4.75AQLSPTFYY44 pKa = 11.21DD45 pKa = 3.62EE46 pKa = 5.17DD47 pKa = 4.37CPNATSIVSAVIEE60 pKa = 4.39EE61 pKa = 4.27ALQTDD66 pKa = 3.76PRR68 pKa = 11.84IAASLTRR75 pKa = 11.84LFFHH79 pKa = 7.45DD80 pKa = 4.76CFVNGCDD87 pKa = 5.1GSVLLDD93 pKa = 3.42NSSTIDD99 pKa = 3.49SEE101 pKa = 4.5KK102 pKa = 11.08GAFANNNSARR112 pKa = 11.84GFDD115 pKa = 3.39VVDD118 pKa = 4.33NIKK121 pKa = 8.85TALEE125 pKa = 4.28TACPGIVSCADD136 pKa = 2.91ILAISAEE143 pKa = 4.21EE144 pKa = 4.12SVSLSGGPSWTVLLGRR160 pKa = 11.84RR161 pKa = 11.84DD162 pKa = 3.3STTANRR168 pKa = 11.84TAANEE173 pKa = 3.93ALPAPSFTLDD183 pKa = 3.1EE184 pKa = 4.75LKK186 pKa = 10.98ASFAAVGLDD195 pKa = 3.37TTDD198 pKa = 4.0LVALSGAHH206 pKa = 4.8TFGRR210 pKa = 11.84AQCQFFSDD218 pKa = 3.33RR219 pKa = 11.84LYY221 pKa = 11.26DD222 pKa = 3.96FNSTGSSDD230 pKa = 3.34PTLNSTYY237 pKa = 10.91LEE239 pKa = 4.27TLSALCPEE247 pKa = 4.64SGNGSVLADD256 pKa = 5.1LDD258 pKa = 4.1PSTPDD263 pKa = 3.71VFDD266 pKa = 5.04ADD268 pKa = 4.11YY269 pKa = 10.82FSNLQVHH276 pKa = 6.19YY277 pKa = 10.74GLLQSDD283 pKa = 4.08QEE285 pKa = 4.36LFSTSGADD293 pKa = 3.03TVDD296 pKa = 3.2IVDD299 pKa = 4.51SYY301 pKa = 11.84SANQSAFFEE310 pKa = 4.62SFVISMNKK318 pKa = 8.25MGNISVLTGTDD329 pKa = 3.3GEE331 pKa = 4.36IRR333 pKa = 11.84LNCSKK338 pKa = 11.16VNGDD342 pKa = 4.0SYY344 pKa = 11.66GSSATLIAEE353 pKa = 4.43YY354 pKa = 10.94

Molecular weight: 37.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6P5ST86|A0A6P5ST86_PRUAV uncharacterized protein LOC110761795 OS=Prunus avium OX=42229 GN=LOC110761795 PE=4 SV=1
MM1 pKa = 7.71GDD3 pKa = 4.1VISKK7 pKa = 10.35AADD10 pKa = 3.45GVGGLLEE17 pKa = 4.33KK18 pKa = 11.0AFLAPFKK25 pKa = 10.69TMFGGSCEE33 pKa = 4.36DD34 pKa = 3.64VCSGPWDD41 pKa = 4.24FGCFIEE47 pKa = 4.39HH48 pKa = 6.75LCVSSLLKK56 pKa = 10.93LLMILVLCYY65 pKa = 9.14ITLLFFYY72 pKa = 10.9LLFKK76 pKa = 11.01LGLFQCIGKK85 pKa = 8.54SLCKK89 pKa = 9.67MCWAACEE96 pKa = 4.32TYY98 pKa = 9.35WYY100 pKa = 10.14ALEE103 pKa = 6.32DD104 pKa = 3.34ITCFLWYY111 pKa = 9.98KK112 pKa = 10.49LKK114 pKa = 9.08NTKK117 pKa = 9.59RR118 pKa = 11.84VNRR121 pKa = 11.84RR122 pKa = 11.84RR123 pKa = 11.84HH124 pKa = 3.9RR125 pKa = 11.84HH126 pKa = 3.99RR127 pKa = 11.84RR128 pKa = 11.84RR129 pKa = 11.84SQFRR133 pKa = 11.84DD134 pKa = 2.91IEE136 pKa = 4.17QGYY139 pKa = 10.17SSSEE143 pKa = 4.1EE144 pKa = 3.46NDD146 pKa = 2.87LWDD149 pKa = 4.4NYY151 pKa = 11.08HH152 pKa = 6.59EE153 pKa = 4.95LNVSRR158 pKa = 11.84KK159 pKa = 8.12RR160 pKa = 11.84KK161 pKa = 8.66SVSRR165 pKa = 11.84RR166 pKa = 11.84RR167 pKa = 11.84KK168 pKa = 10.02DD169 pKa = 3.21KK170 pKa = 10.74LQSSVYY176 pKa = 8.36PSSRR180 pKa = 11.84RR181 pKa = 11.84GTHH184 pKa = 4.41SHH186 pKa = 4.68HH187 pKa = 6.47HH188 pKa = 5.74VRR190 pKa = 11.84LKK192 pKa = 10.69SRR194 pKa = 11.84GRR196 pKa = 11.84SVRR199 pKa = 11.84IGGRR203 pKa = 11.84SQRR206 pKa = 11.84PRR208 pKa = 11.84SSRR211 pKa = 11.84HH212 pKa = 3.93LQLRR216 pKa = 11.84KK217 pKa = 9.42VRR219 pKa = 11.84NVRR222 pKa = 11.84RR223 pKa = 11.84QARR226 pKa = 11.84TSKK229 pKa = 10.11RR230 pKa = 11.84RR231 pKa = 11.84KK232 pKa = 9.53LRR234 pKa = 3.61

Molecular weight: 27.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

25598

4917

30515

14032431

37

5357

459.9

51.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.665 ± 0.013

1.884 ± 0.007

5.303 ± 0.009

6.474 ± 0.015

4.201 ± 0.009

6.526 ± 0.012

2.45 ± 0.006

5.247 ± 0.009

6.109 ± 0.011

9.892 ± 0.018

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.366 ± 0.006

4.527 ± 0.009

4.915 ± 0.013

3.795 ± 0.008

5.17 ± 0.01

8.979 ± 0.015

4.891 ± 0.008

6.508 ± 0.009

1.294 ± 0.005

2.798 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski