Mycobacterium phage LilDestine

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bronvirus; unclassified Bronvirus

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 129 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A386KJ48|A0A386KJ48_9CAUD Uncharacterized protein OS=Mycobacterium phage LilDestine OX=2315542 GN=66 PE=4 SV=1
MM1 pKa = 7.37VDD3 pKa = 4.18CVDD6 pKa = 4.31HH7 pKa = 6.52NHH9 pKa = 5.96TDD11 pKa = 3.29EE12 pKa = 5.56LGACEE17 pKa = 4.58GVVSLYY23 pKa = 11.16ASLSGSGEE31 pKa = 4.12SYY33 pKa = 10.25PRR35 pKa = 11.84CEE37 pKa = 5.32RR38 pKa = 11.84PPDD41 pKa = 3.55WFDD44 pKa = 3.08PTYY47 pKa = 11.01AGEE50 pKa = 4.21RR51 pKa = 11.84WNEE54 pKa = 3.6DD55 pKa = 3.28DD56 pKa = 4.71

Molecular weight: 6.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A386KFM9|A0A386KFM9_9CAUD Lysin B OS=Mycobacterium phage LilDestine OX=2315542 GN=28 PE=4 SV=1
MM1 pKa = 6.83VQTVTPTTSQTSAHH15 pKa = 6.23TIRR18 pKa = 11.84WIPEE22 pKa = 3.54AVQNLLHH29 pKa = 6.64NNDD32 pKa = 2.75ILTRR36 pKa = 11.84TEE38 pKa = 3.61LAKK41 pKa = 10.92ALGVARR47 pKa = 11.84STVYY51 pKa = 9.25RR52 pKa = 11.84TFDD55 pKa = 3.81EE56 pKa = 4.67DD57 pKa = 3.32WSGEE61 pKa = 3.83PSMTMLATMAGHH73 pKa = 6.55FRR75 pKa = 11.84VPLNRR80 pKa = 11.84IVTEE84 pKa = 4.13PGSAVARR91 pKa = 11.84RR92 pKa = 11.84RR93 pKa = 11.84TVRR96 pKa = 11.84LAGG99 pKa = 3.58

Molecular weight: 10.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

129

0

129

22895

32

1709

177.5

19.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.649 ± 0.416

1.057 ± 0.157

6.416 ± 0.18

6.049 ± 0.274

3.202 ± 0.121

8.504 ± 0.443

1.931 ± 0.142

4.154 ± 0.173

4.979 ± 0.3

8.342 ± 0.215

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.324 ± 0.116

3.625 ± 0.147

5.093 ± 0.261

3.617 ± 0.18

6.373 ± 0.223

5.49 ± 0.206

5.822 ± 0.256

7.456 ± 0.307

2.105 ± 0.177

2.813 ± 0.139
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski