Amino acid dipepetide frequency for Mycobacterium phage LilDestine

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.885AlaAla: 12.885 ± 1.129
0.917AlaCys: 0.917 ± 0.207
6.202AlaAsp: 6.202 ± 0.518
7.6AlaGlu: 7.6 ± 0.684
3.363AlaPhe: 3.363 ± 0.37
8.037AlaGly: 8.037 ± 0.86
1.704AlaHis: 1.704 ± 0.276
4.848AlaIle: 4.848 ± 0.446
6.29AlaLys: 6.29 ± 0.58
9.478AlaLeu: 9.478 ± 0.514
3.276AlaMet: 3.276 ± 0.397
3.625AlaAsn: 3.625 ± 0.5
4.324AlaPro: 4.324 ± 0.575
4.193AlaGln: 4.193 ± 0.655
5.635AlaArg: 5.635 ± 0.438
5.504AlaSer: 5.504 ± 0.63
6.202AlaThr: 6.202 ± 0.663
8.255AlaVal: 8.255 ± 0.868
1.572AlaTrp: 1.572 ± 0.253
2.577AlaTyr: 2.577 ± 0.287
0.0AlaXaa: 0.0 ± 0.0
Cys
0.917CysAla: 0.917 ± 0.281
0.218CysCys: 0.218 ± 0.118
0.917CysAsp: 0.917 ± 0.199
0.699CysGlu: 0.699 ± 0.188
0.306CysPhe: 0.306 ± 0.14
1.354CysGly: 1.354 ± 0.306
0.131CysHis: 0.131 ± 0.078
0.306CysIle: 0.306 ± 0.104
0.175CysLys: 0.175 ± 0.09
0.568CysLeu: 0.568 ± 0.166
0.437CysMet: 0.437 ± 0.146
0.349CysAsn: 0.349 ± 0.137
0.48CysPro: 0.48 ± 0.136
0.393CysGln: 0.393 ± 0.126
0.699CysArg: 0.699 ± 0.223
0.524CysSer: 0.524 ± 0.154
0.655CysThr: 0.655 ± 0.174
1.048CysVal: 1.048 ± 0.376
0.175CysTrp: 0.175 ± 0.087
0.218CysTyr: 0.218 ± 0.095
0.0CysXaa: 0.0 ± 0.0
Asp
5.591AspAla: 5.591 ± 0.741
0.917AspCys: 0.917 ± 0.222
4.717AspAsp: 4.717 ± 0.468
4.062AspGlu: 4.062 ± 0.48
2.097AspPhe: 2.097 ± 0.279
5.897AspGly: 5.897 ± 0.53
1.048AspHis: 1.048 ± 0.206
2.883AspIle: 2.883 ± 0.362
3.058AspLys: 3.058 ± 0.353
5.504AspLeu: 5.504 ± 0.425
1.747AspMet: 1.747 ± 0.296
1.835AspAsn: 1.835 ± 0.307
3.931AspPro: 3.931 ± 0.434
2.184AspGln: 2.184 ± 0.238
4.324AspArg: 4.324 ± 0.475
3.363AspSer: 3.363 ± 0.428
3.101AspThr: 3.101 ± 0.382
4.062AspVal: 4.062 ± 0.398
1.529AspTrp: 1.529 ± 0.275
2.315AspTyr: 2.315 ± 0.378
0.0AspXaa: 0.0 ± 0.0
Glu
6.77GluAla: 6.77 ± 0.71
0.612GluCys: 0.612 ± 0.178
2.927GluAsp: 2.927 ± 0.337
2.402GluGlu: 2.402 ± 0.414
1.878GluPhe: 1.878 ± 0.284
4.15GluGly: 4.15 ± 0.45
2.053GluHis: 2.053 ± 0.278
2.621GluIle: 2.621 ± 0.335
2.621GluLys: 2.621 ± 0.455
6.945GluLeu: 6.945 ± 0.594
1.398GluMet: 1.398 ± 0.202
2.402GluAsn: 2.402 ± 0.381
2.927GluPro: 2.927 ± 0.429
2.315GluGln: 2.315 ± 0.266
5.067GluArg: 5.067 ± 0.598
2.533GluSer: 2.533 ± 0.33
3.8GluThr: 3.8 ± 0.522
4.237GluVal: 4.237 ± 0.408
1.048GluTrp: 1.048 ± 0.241
1.791GluTyr: 1.791 ± 0.26
0.0GluXaa: 0.0 ± 0.0
Phe
2.752PheAla: 2.752 ± 0.32
0.393PheCys: 0.393 ± 0.135
1.966PheAsp: 1.966 ± 0.349
1.878PheGlu: 1.878 ± 0.247
1.223PhePhe: 1.223 ± 0.162
3.232PheGly: 3.232 ± 0.41
0.83PheHis: 0.83 ± 0.173
1.398PheIle: 1.398 ± 0.238
1.835PheLys: 1.835 ± 0.283
2.795PheLeu: 2.795 ± 0.46
0.874PheMet: 0.874 ± 0.205
1.267PheAsn: 1.267 ± 0.194
1.354PhePro: 1.354 ± 0.253
1.005PheGln: 1.005 ± 0.207
1.616PheArg: 1.616 ± 0.305
1.791PheSer: 1.791 ± 0.268
1.922PheThr: 1.922 ± 0.248
2.228PheVal: 2.228 ± 0.257
0.48PheTrp: 0.48 ± 0.119
1.179PheTyr: 1.179 ± 0.197
0.0PheXaa: 0.0 ± 0.0
Gly
7.688GlyAla: 7.688 ± 1.07
0.874GlyCys: 0.874 ± 0.245
5.591GlyAsp: 5.591 ± 0.489
5.591GlyGlu: 5.591 ± 0.46
2.752GlyPhe: 2.752 ± 0.414
8.692GlyGly: 8.692 ± 1.208
1.791GlyHis: 1.791 ± 0.315
4.324GlyIle: 4.324 ± 0.415
5.067GlyLys: 5.067 ± 0.48
6.989GlyLeu: 6.989 ± 0.832
1.791GlyMet: 1.791 ± 0.211
2.271GlyAsn: 2.271 ± 0.343
3.189GlyPro: 3.189 ± 0.365
2.49GlyGln: 2.49 ± 0.393
4.674GlyArg: 4.674 ± 0.403
5.242GlySer: 5.242 ± 0.616
5.373GlyThr: 5.373 ± 0.603
6.246GlyVal: 6.246 ± 0.614
1.529GlyTrp: 1.529 ± 0.247
2.883GlyTyr: 2.883 ± 0.391
0.0GlyXaa: 0.0 ± 0.0
His
1.922HisAla: 1.922 ± 0.246
0.131HisCys: 0.131 ± 0.064
0.917HisAsp: 0.917 ± 0.208
1.223HisGlu: 1.223 ± 0.259
0.961HisPhe: 0.961 ± 0.19
1.572HisGly: 1.572 ± 0.21
0.262HisHis: 0.262 ± 0.123
0.874HisIle: 0.874 ± 0.25
0.786HisLys: 0.786 ± 0.178
1.791HisLeu: 1.791 ± 0.29
0.524HisMet: 0.524 ± 0.15
0.524HisAsn: 0.524 ± 0.138
1.136HisPro: 1.136 ± 0.201
0.524HisGln: 0.524 ± 0.147
1.354HisArg: 1.354 ± 0.235
0.874HisSer: 0.874 ± 0.228
1.005HisThr: 1.005 ± 0.184
1.922HisVal: 1.922 ± 0.341
0.393HisTrp: 0.393 ± 0.136
0.612HisTyr: 0.612 ± 0.175
0.0HisXaa: 0.0 ± 0.0
Ile
4.848IleAla: 4.848 ± 0.534
0.349IleCys: 0.349 ± 0.149
2.927IleAsp: 2.927 ± 0.334
3.232IleGlu: 3.232 ± 0.414
0.917IlePhe: 0.917 ± 0.169
3.276IleGly: 3.276 ± 0.387
0.699IleHis: 0.699 ± 0.184
1.704IleIle: 1.704 ± 0.247
2.359IleLys: 2.359 ± 0.346
2.927IleLeu: 2.927 ± 0.306
0.786IleMet: 0.786 ± 0.171
1.529IleAsn: 1.529 ± 0.26
2.446IlePro: 2.446 ± 0.306
1.529IleGln: 1.529 ± 0.367
2.752IleArg: 2.752 ± 0.364
1.966IleSer: 1.966 ± 0.24
2.533IleThr: 2.533 ± 0.341
2.97IleVal: 2.97 ± 0.377
0.786IleTrp: 0.786 ± 0.201
1.005IleTyr: 1.005 ± 0.21
0.0IleXaa: 0.0 ± 0.0
Lys
6.596LysAla: 6.596 ± 0.71
0.524LysCys: 0.524 ± 0.158
3.058LysAsp: 3.058 ± 0.369
2.009LysGlu: 2.009 ± 0.309
1.791LysPhe: 1.791 ± 0.301
4.237LysGly: 4.237 ± 0.454
1.136LysHis: 1.136 ± 0.223
1.441LysIle: 1.441 ± 0.289
2.271LysLys: 2.271 ± 0.372
3.713LysLeu: 3.713 ± 0.49
0.874LysMet: 0.874 ± 0.194
2.228LysAsn: 2.228 ± 0.331
2.795LysPro: 2.795 ± 0.473
1.966LysGln: 1.966 ± 0.298
4.761LysArg: 4.761 ± 0.501
2.184LysSer: 2.184 ± 0.358
2.315LysThr: 2.315 ± 0.367
3.101LysVal: 3.101 ± 0.366
1.441LysTrp: 1.441 ± 0.224
1.354LysTyr: 1.354 ± 0.239
0.0LysXaa: 0.0 ± 0.0
Leu
8.954LeuAla: 8.954 ± 0.596
0.786LeuCys: 0.786 ± 0.286
5.94LeuAsp: 5.94 ± 0.42
4.674LeuGlu: 4.674 ± 0.528
2.664LeuPhe: 2.664 ± 0.348
5.591LeuGly: 5.591 ± 0.382
1.485LeuHis: 1.485 ± 0.297
3.363LeuIle: 3.363 ± 0.52
4.455LeuLys: 4.455 ± 0.378
6.552LeuLeu: 6.552 ± 0.457
1.878LeuMet: 1.878 ± 0.259
3.494LeuAsn: 3.494 ± 0.489
4.892LeuPro: 4.892 ± 0.537
3.101LeuGln: 3.101 ± 0.411
5.591LeuArg: 5.591 ± 0.43
5.504LeuSer: 5.504 ± 0.681
4.979LeuThr: 4.979 ± 0.479
5.722LeuVal: 5.722 ± 0.477
2.14LeuTrp: 2.14 ± 0.375
1.66LeuTyr: 1.66 ± 0.298
0.0LeuXaa: 0.0 ± 0.0
Met
2.883MetAla: 2.883 ± 0.384
0.306MetCys: 0.306 ± 0.115
1.136MetAsp: 1.136 ± 0.227
1.267MetGlu: 1.267 ± 0.261
0.655MetPhe: 0.655 ± 0.175
1.529MetGly: 1.529 ± 0.238
0.437MetHis: 0.437 ± 0.124
0.655MetIle: 0.655 ± 0.196
0.917MetLys: 0.917 ± 0.167
1.835MetLeu: 1.835 ± 0.302
0.349MetMet: 0.349 ± 0.119
0.655MetAsn: 0.655 ± 0.179
1.398MetPro: 1.398 ± 0.247
0.786MetGln: 0.786 ± 0.207
1.398MetArg: 1.398 ± 0.263
2.446MetSer: 2.446 ± 0.358
1.922MetThr: 1.922 ± 0.254
1.398MetVal: 1.398 ± 0.205
0.524MetTrp: 0.524 ± 0.164
0.743MetTyr: 0.743 ± 0.196
0.0MetXaa: 0.0 ± 0.0
Asn
4.499AsnAla: 4.499 ± 0.434
0.131AsnCys: 0.131 ± 0.079
2.446AsnAsp: 2.446 ± 0.317
1.835AsnGlu: 1.835 ± 0.26
1.005AsnPhe: 1.005 ± 0.194
3.494AsnGly: 3.494 ± 0.372
0.917AsnHis: 0.917 ± 0.191
1.441AsnIle: 1.441 ± 0.289
1.485AsnLys: 1.485 ± 0.295
3.145AsnLeu: 3.145 ± 0.341
0.743AsnMet: 0.743 ± 0.182
1.572AsnAsn: 1.572 ± 0.381
2.664AsnPro: 2.664 ± 0.337
1.354AsnGln: 1.354 ± 0.236
2.271AsnArg: 2.271 ± 0.368
1.398AsnSer: 1.398 ± 0.258
1.835AsnThr: 1.835 ± 0.277
2.49AsnVal: 2.49 ± 0.271
0.743AsnTrp: 0.743 ± 0.156
0.743AsnTyr: 0.743 ± 0.174
0.0AsnXaa: 0.0 ± 0.0
Pro
4.63ProAla: 4.63 ± 0.514
0.655ProCys: 0.655 ± 0.224
3.8ProAsp: 3.8 ± 0.421
3.8ProGlu: 3.8 ± 0.439
1.572ProPhe: 1.572 ± 0.344
4.848ProGly: 4.848 ± 0.911
0.655ProHis: 0.655 ± 0.162
1.572ProIle: 1.572 ± 0.223
2.577ProLys: 2.577 ± 0.427
3.538ProLeu: 3.538 ± 0.434
1.223ProMet: 1.223 ± 0.233
2.402ProAsn: 2.402 ± 0.359
2.621ProPro: 2.621 ± 0.419
1.791ProGln: 1.791 ± 0.295
2.533ProArg: 2.533 ± 0.286
2.795ProSer: 2.795 ± 0.325
3.451ProThr: 3.451 ± 0.499
3.975ProVal: 3.975 ± 0.386
1.354ProTrp: 1.354 ± 0.255
1.136ProTyr: 1.136 ± 0.239
0.0ProXaa: 0.0 ± 0.0
Gln
4.674GlnAla: 4.674 ± 0.443
0.437GlnCys: 0.437 ± 0.157
1.66GlnAsp: 1.66 ± 0.299
1.835GlnGlu: 1.835 ± 0.268
1.616GlnPhe: 1.616 ± 0.266
2.446GlnGly: 2.446 ± 0.301
0.568GlnHis: 0.568 ± 0.179
2.053GlnIle: 2.053 ± 0.264
1.485GlnLys: 1.485 ± 0.287
2.795GlnLeu: 2.795 ± 0.425
0.786GlnMet: 0.786 ± 0.213
1.354GlnAsn: 1.354 ± 0.215
1.835GlnPro: 1.835 ± 0.283
1.485GlnGln: 1.485 ± 0.394
2.883GlnArg: 2.883 ± 0.275
1.572GlnSer: 1.572 ± 0.252
1.791GlnThr: 1.791 ± 0.273
3.32GlnVal: 3.32 ± 0.375
0.655GlnTrp: 0.655 ± 0.154
0.917GlnTyr: 0.917 ± 0.209
0.0GlnXaa: 0.0 ± 0.0
Arg
6.901ArgAla: 6.901 ± 0.667
1.136ArgCys: 1.136 ± 0.378
4.237ArgAsp: 4.237 ± 0.413
4.848ArgGlu: 4.848 ± 0.427
1.791ArgPhe: 1.791 ± 0.279
5.242ArgGly: 5.242 ± 0.49
1.223ArgHis: 1.223 ± 0.194
2.49ArgIle: 2.49 ± 0.284
4.281ArgLys: 4.281 ± 0.641
5.023ArgLeu: 5.023 ± 0.409
1.179ArgMet: 1.179 ± 0.238
2.315ArgAsn: 2.315 ± 0.325
2.402ArgPro: 2.402 ± 0.383
2.402ArgGln: 2.402 ± 0.282
3.669ArgArg: 3.669 ± 0.391
3.32ArgSer: 3.32 ± 0.374
3.363ArgThr: 3.363 ± 0.405
4.674ArgVal: 4.674 ± 0.414
1.529ArgTrp: 1.529 ± 0.278
1.704ArgTyr: 1.704 ± 0.281
0.0ArgXaa: 0.0 ± 0.0
Ser
5.678SerAla: 5.678 ± 0.5
0.262SerCys: 0.262 ± 0.104
3.494SerAsp: 3.494 ± 0.383
3.538SerGlu: 3.538 ± 0.402
1.704SerPhe: 1.704 ± 0.346
6.377SerGly: 6.377 ± 0.995
1.136SerHis: 1.136 ± 0.212
2.446SerIle: 2.446 ± 0.306
2.708SerLys: 2.708 ± 0.358
4.368SerLeu: 4.368 ± 0.436
1.529SerMet: 1.529 ± 0.245
1.66SerAsn: 1.66 ± 0.197
2.49SerPro: 2.49 ± 0.298
1.791SerGln: 1.791 ± 0.265
2.795SerArg: 2.795 ± 0.401
2.883SerSer: 2.883 ± 0.396
2.752SerThr: 2.752 ± 0.329
4.106SerVal: 4.106 ± 0.403
1.267SerTrp: 1.267 ± 0.248
1.922SerTyr: 1.922 ± 0.262
0.0SerXaa: 0.0 ± 0.0
Thr
6.115ThrAla: 6.115 ± 0.669
0.306ThrCys: 0.306 ± 0.117
3.625ThrAsp: 3.625 ± 0.407
2.883ThrGlu: 2.883 ± 0.368
2.402ThrPhe: 2.402 ± 0.306
5.198ThrGly: 5.198 ± 0.575
0.961ThrHis: 0.961 ± 0.187
2.708ThrIle: 2.708 ± 0.49
2.315ThrLys: 2.315 ± 0.357
5.635ThrLeu: 5.635 ± 0.439
0.961ThrMet: 0.961 ± 0.256
2.053ThrAsn: 2.053 ± 0.313
3.363ThrPro: 3.363 ± 0.429
1.966ThrGln: 1.966 ± 0.295
3.014ThrArg: 3.014 ± 0.359
2.97ThrSer: 2.97 ± 0.419
2.97ThrThr: 2.97 ± 0.406
5.678ThrVal: 5.678 ± 0.665
1.048ThrTrp: 1.048 ± 0.263
2.053ThrTyr: 2.053 ± 0.297
0.0ThrXaa: 0.0 ± 0.0
Val
7.469ValAla: 7.469 ± 0.768
1.092ValCys: 1.092 ± 0.22
5.154ValAsp: 5.154 ± 0.526
4.15ValGlu: 4.15 ± 0.385
1.704ValPhe: 1.704 ± 0.345
5.766ValGly: 5.766 ± 0.637
1.223ValHis: 1.223 ± 0.218
2.883ValIle: 2.883 ± 0.455
3.582ValLys: 3.582 ± 0.428
5.504ValLeu: 5.504 ± 0.474
1.529ValMet: 1.529 ± 0.266
2.402ValAsn: 2.402 ± 0.341
4.324ValPro: 4.324 ± 0.462
2.752ValGln: 2.752 ± 0.406
5.023ValArg: 5.023 ± 0.427
5.242ValSer: 5.242 ± 0.434
5.198ValThr: 5.198 ± 0.644
5.897ValVal: 5.897 ± 0.869
1.529ValTrp: 1.529 ± 0.261
2.14ValTyr: 2.14 ± 0.34
0.0ValXaa: 0.0 ± 0.0
Trp
1.966TrpAla: 1.966 ± 0.296
0.175TrpCys: 0.175 ± 0.077
1.441TrpAsp: 1.441 ± 0.301
1.092TrpGlu: 1.092 ± 0.246
0.48TrpPhe: 0.48 ± 0.148
1.179TrpGly: 1.179 ± 0.222
0.655TrpHis: 0.655 ± 0.165
0.874TrpIle: 0.874 ± 0.192
0.655TrpLys: 0.655 ± 0.181
2.228TrpLeu: 2.228 ± 0.333
0.655TrpMet: 0.655 ± 0.162
1.179TrpAsn: 1.179 ± 0.197
0.917TrpPro: 0.917 ± 0.194
0.961TrpGln: 0.961 ± 0.224
1.485TrpArg: 1.485 ± 0.227
1.179TrpSer: 1.179 ± 0.234
1.616TrpThr: 1.616 ± 0.357
1.398TrpVal: 1.398 ± 0.258
0.568TrpTrp: 0.568 ± 0.192
0.349TrpTyr: 0.349 ± 0.134
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.752TyrAla: 2.752 ± 0.42
0.349TyrCys: 0.349 ± 0.153
2.009TyrAsp: 2.009 ± 0.303
1.878TyrGlu: 1.878 ± 0.256
1.136TyrPhe: 1.136 ± 0.228
2.927TyrGly: 2.927 ± 0.384
0.393TyrHis: 0.393 ± 0.133
0.655TyrIle: 0.655 ± 0.163
0.874TyrLys: 0.874 ± 0.194
2.097TyrLeu: 2.097 ± 0.279
0.655TyrMet: 0.655 ± 0.166
1.136TyrAsn: 1.136 ± 0.18
1.441TyrPro: 1.441 ± 0.253
1.179TyrGln: 1.179 ± 0.223
2.184TyrArg: 2.184 ± 0.289
1.616TyrSer: 1.616 ± 0.319
1.441TyrThr: 1.441 ± 0.239
1.835TyrVal: 1.835 ± 0.278
0.743TyrTrp: 0.743 ± 0.178
0.83TyrTyr: 0.83 ± 0.191
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 129 proteins (22895 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski