Amino acid dipepetide frequency for Mycobacterium phage Nyxis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.962AlaAla: 10.962 ± 1.039
0.747AlaCys: 0.747 ± 0.23
4.485AlaAsp: 4.485 ± 0.505
6.229AlaGlu: 6.229 ± 0.613
2.927AlaPhe: 2.927 ± 0.364
8.159AlaGly: 8.159 ± 0.802
1.744AlaHis: 1.744 ± 0.341
5.107AlaIle: 5.107 ± 0.563
3.986AlaLys: 3.986 ± 0.617
9.031AlaLeu: 9.031 ± 0.983
3.052AlaMet: 3.052 ± 0.535
3.426AlaAsn: 3.426 ± 0.531
5.232AlaPro: 5.232 ± 0.807
3.737AlaGln: 3.737 ± 0.54
6.042AlaArg: 6.042 ± 0.737
4.049AlaSer: 4.049 ± 0.623
5.543AlaThr: 5.543 ± 0.549
7.599AlaVal: 7.599 ± 0.661
2.055AlaTrp: 2.055 ± 0.394
2.242AlaTyr: 2.242 ± 0.504
0.0AlaXaa: 0.0 ± 0.0
Cys
0.685CysAla: 0.685 ± 0.226
0.187CysCys: 0.187 ± 0.134
0.623CysAsp: 0.623 ± 0.265
0.623CysGlu: 0.623 ± 0.181
0.311CysPhe: 0.311 ± 0.133
0.685CysGly: 0.685 ± 0.186
0.311CysHis: 0.311 ± 0.128
0.125CysIle: 0.125 ± 0.086
0.311CysLys: 0.311 ± 0.114
0.81CysLeu: 0.81 ± 0.24
0.125CysMet: 0.125 ± 0.086
0.436CysAsn: 0.436 ± 0.145
0.561CysPro: 0.561 ± 0.176
0.187CysGln: 0.187 ± 0.101
0.623CysArg: 0.623 ± 0.208
0.187CysSer: 0.187 ± 0.117
0.374CysThr: 0.374 ± 0.166
0.623CysVal: 0.623 ± 0.184
0.249CysTrp: 0.249 ± 0.14
0.249CysTyr: 0.249 ± 0.136
0.0CysXaa: 0.0 ± 0.0
Asp
6.229AspAla: 6.229 ± 0.584
0.498AspCys: 0.498 ± 0.159
3.363AspAsp: 3.363 ± 0.468
3.862AspGlu: 3.862 ± 0.438
1.931AspPhe: 1.931 ± 0.395
5.917AspGly: 5.917 ± 0.631
1.495AspHis: 1.495 ± 0.316
2.865AspIle: 2.865 ± 0.501
2.429AspLys: 2.429 ± 0.414
5.73AspLeu: 5.73 ± 0.56
1.183AspMet: 1.183 ± 0.244
1.806AspAsn: 1.806 ± 0.39
4.671AspPro: 4.671 ± 0.628
1.806AspGln: 1.806 ± 0.31
3.737AspArg: 3.737 ± 0.474
3.114AspSer: 3.114 ± 0.446
3.426AspThr: 3.426 ± 0.533
4.235AspVal: 4.235 ± 0.433
1.433AspTrp: 1.433 ± 0.336
2.491AspTyr: 2.491 ± 0.345
0.0AspXaa: 0.0 ± 0.0
Glu
6.166GluAla: 6.166 ± 0.615
0.685GluCys: 0.685 ± 0.198
4.547GluAsp: 4.547 ± 0.56
4.858GluGlu: 4.858 ± 0.693
2.491GluPhe: 2.491 ± 0.375
5.543GluGly: 5.543 ± 0.712
1.121GluHis: 1.121 ± 0.289
2.118GluIle: 2.118 ± 0.431
2.305GluLys: 2.305 ± 0.354
7.537GluLeu: 7.537 ± 0.777
1.121GluMet: 1.121 ± 0.304
2.305GluAsn: 2.305 ± 0.521
3.177GluPro: 3.177 ± 0.544
2.367GluGln: 2.367 ± 0.314
4.983GluArg: 4.983 ± 0.594
3.986GluSer: 3.986 ± 0.517
2.865GluThr: 2.865 ± 0.425
4.734GluVal: 4.734 ± 0.612
1.433GluTrp: 1.433 ± 0.314
1.619GluTyr: 1.619 ± 0.354
0.0GluXaa: 0.0 ± 0.0
Phe
3.363PheAla: 3.363 ± 0.461
0.249PheCys: 0.249 ± 0.119
2.554PheAsp: 2.554 ± 0.363
2.429PheGlu: 2.429 ± 0.496
0.561PhePhe: 0.561 ± 0.171
3.363PheGly: 3.363 ± 0.436
0.561PheHis: 0.561 ± 0.195
1.993PheIle: 1.993 ± 0.317
1.121PheLys: 1.121 ± 0.228
2.865PheLeu: 2.865 ± 0.549
0.685PheMet: 0.685 ± 0.236
1.121PheAsn: 1.121 ± 0.331
1.931PhePro: 1.931 ± 0.369
0.997PheGln: 0.997 ± 0.251
1.619PheArg: 1.619 ± 0.246
1.744PheSer: 1.744 ± 0.329
1.682PheThr: 1.682 ± 0.336
2.678PheVal: 2.678 ± 0.465
0.311PheTrp: 0.311 ± 0.145
0.623PheTyr: 0.623 ± 0.174
0.0PheXaa: 0.0 ± 0.0
Gly
5.668GlyAla: 5.668 ± 0.608
0.81GlyCys: 0.81 ± 0.231
6.166GlyAsp: 6.166 ± 0.913
4.796GlyGlu: 4.796 ± 0.624
3.924GlyPhe: 3.924 ± 0.572
8.658GlyGly: 8.658 ± 1.601
2.242GlyHis: 2.242 ± 0.383
4.485GlyIle: 4.485 ± 0.649
4.547GlyLys: 4.547 ± 0.523
7.101GlyLeu: 7.101 ± 0.803
2.242GlyMet: 2.242 ± 0.337
3.675GlyAsn: 3.675 ± 0.731
6.353GlyPro: 6.353 ± 2.206
2.678GlyGln: 2.678 ± 0.432
5.107GlyArg: 5.107 ± 0.598
4.921GlySer: 4.921 ± 0.65
5.294GlyThr: 5.294 ± 0.804
6.291GlyVal: 6.291 ± 0.695
2.118GlyTrp: 2.118 ± 0.354
2.803GlyTyr: 2.803 ± 0.436
0.0GlyXaa: 0.0 ± 0.0
His
1.806HisAla: 1.806 ± 0.313
0.125HisCys: 0.125 ± 0.093
1.059HisAsp: 1.059 ± 0.287
1.495HisGlu: 1.495 ± 0.372
0.81HisPhe: 0.81 ± 0.239
1.869HisGly: 1.869 ± 0.364
0.498HisHis: 0.498 ± 0.18
0.872HisIle: 0.872 ± 0.181
0.872HisLys: 0.872 ± 0.254
1.744HisLeu: 1.744 ± 0.32
0.436HisMet: 0.436 ± 0.159
0.311HisAsn: 0.311 ± 0.13
1.495HisPro: 1.495 ± 0.267
0.685HisGln: 0.685 ± 0.204
2.055HisArg: 2.055 ± 0.368
0.561HisSer: 0.561 ± 0.22
1.246HisThr: 1.246 ± 0.336
0.934HisVal: 0.934 ± 0.213
0.561HisTrp: 0.561 ± 0.202
0.685HisTyr: 0.685 ± 0.209
0.0HisXaa: 0.0 ± 0.0
Ile
5.481IleAla: 5.481 ± 0.637
0.249IleCys: 0.249 ± 0.097
3.675IleAsp: 3.675 ± 0.44
3.426IleGlu: 3.426 ± 0.499
0.747IlePhe: 0.747 ± 0.219
4.049IleGly: 4.049 ± 0.523
0.872IleHis: 0.872 ± 0.213
1.619IleIle: 1.619 ± 0.311
1.557IleLys: 1.557 ± 0.302
3.675IleLeu: 3.675 ± 0.454
0.747IleMet: 0.747 ± 0.218
1.246IleAsn: 1.246 ± 0.267
3.737IlePro: 3.737 ± 0.394
1.495IleGln: 1.495 ± 0.427
3.799IleArg: 3.799 ± 0.391
1.806IleSer: 1.806 ± 0.35
3.114IleThr: 3.114 ± 0.428
3.55IleVal: 3.55 ± 0.462
0.436IleTrp: 0.436 ± 0.185
1.246IleTyr: 1.246 ± 0.262
0.0IleXaa: 0.0 ± 0.0
Lys
4.609LysAla: 4.609 ± 0.621
0.187LysCys: 0.187 ± 0.104
2.616LysAsp: 2.616 ± 0.434
2.367LysGlu: 2.367 ± 0.418
1.246LysPhe: 1.246 ± 0.301
4.173LysGly: 4.173 ± 0.705
1.246LysHis: 1.246 ± 0.286
2.118LysIle: 2.118 ± 0.605
2.055LysLys: 2.055 ± 0.397
3.924LysLeu: 3.924 ± 0.375
0.436LysMet: 0.436 ± 0.201
1.495LysAsn: 1.495 ± 0.243
2.616LysPro: 2.616 ± 0.467
1.619LysGln: 1.619 ± 0.304
3.177LysArg: 3.177 ± 0.556
2.367LysSer: 2.367 ± 0.323
2.616LysThr: 2.616 ± 0.39
2.927LysVal: 2.927 ± 0.419
0.872LysTrp: 0.872 ± 0.24
1.308LysTyr: 1.308 ± 0.252
0.0LysXaa: 0.0 ± 0.0
Leu
8.907LeuAla: 8.907 ± 0.754
0.747LeuCys: 0.747 ± 0.213
5.543LeuAsp: 5.543 ± 0.542
6.042LeuGlu: 6.042 ± 0.654
2.491LeuPhe: 2.491 ± 0.405
7.038LeuGly: 7.038 ± 0.709
1.183LeuHis: 1.183 ± 0.331
4.298LeuIle: 4.298 ± 0.449
3.239LeuLys: 3.239 ± 0.627
6.291LeuLeu: 6.291 ± 0.741
2.927LeuMet: 2.927 ± 0.51
2.803LeuAsn: 2.803 ± 0.367
4.36LeuPro: 4.36 ± 0.412
2.678LeuGln: 2.678 ± 0.408
6.727LeuArg: 6.727 ± 0.636
5.668LeuSer: 5.668 ± 0.664
4.734LeuThr: 4.734 ± 0.581
5.232LeuVal: 5.232 ± 0.567
1.246LeuTrp: 1.246 ± 0.291
2.429LeuTyr: 2.429 ± 0.386
0.0LeuXaa: 0.0 ± 0.0
Met
2.367MetAla: 2.367 ± 0.302
0.062MetCys: 0.062 ± 0.063
1.682MetAsp: 1.682 ± 0.371
1.121MetGlu: 1.121 ± 0.229
0.934MetPhe: 0.934 ± 0.287
1.869MetGly: 1.869 ± 0.386
0.498MetHis: 0.498 ± 0.16
0.934MetIle: 0.934 ± 0.282
1.682MetLys: 1.682 ± 0.314
1.433MetLeu: 1.433 ± 0.283
0.311MetMet: 0.311 ± 0.128
0.81MetAsn: 0.81 ± 0.189
1.744MetPro: 1.744 ± 0.31
0.81MetGln: 0.81 ± 0.203
1.806MetArg: 1.806 ± 0.365
1.869MetSer: 1.869 ± 0.335
2.055MetThr: 2.055 ± 0.289
1.495MetVal: 1.495 ± 0.261
0.498MetTrp: 0.498 ± 0.186
0.623MetTyr: 0.623 ± 0.204
0.0MetXaa: 0.0 ± 0.0
Asn
3.488AsnAla: 3.488 ± 0.502
0.311AsnCys: 0.311 ± 0.148
1.806AsnAsp: 1.806 ± 0.339
1.37AsnGlu: 1.37 ± 0.24
0.561AsnPhe: 0.561 ± 0.171
3.924AsnGly: 3.924 ± 0.512
0.747AsnHis: 0.747 ± 0.187
1.37AsnIle: 1.37 ± 0.332
1.37AsnLys: 1.37 ± 0.245
2.491AsnLeu: 2.491 ± 0.391
0.747AsnMet: 0.747 ± 0.255
0.498AsnAsn: 0.498 ± 0.2
2.367AsnPro: 2.367 ± 0.468
1.246AsnGln: 1.246 ± 0.295
2.305AsnArg: 2.305 ± 0.312
2.118AsnSer: 2.118 ± 0.403
1.931AsnThr: 1.931 ± 0.306
2.491AsnVal: 2.491 ± 0.333
0.872AsnTrp: 0.872 ± 0.222
0.997AsnTyr: 0.997 ± 0.252
0.0AsnXaa: 0.0 ± 0.0
Pro
5.232ProAla: 5.232 ± 0.634
0.498ProCys: 0.498 ± 0.187
2.927ProAsp: 2.927 ± 0.404
4.111ProGlu: 4.111 ± 0.479
1.869ProPhe: 1.869 ± 0.289
5.481ProGly: 5.481 ± 0.574
1.059ProHis: 1.059 ± 0.247
2.242ProIle: 2.242 ± 0.337
2.491ProLys: 2.491 ± 0.514
3.986ProLeu: 3.986 ± 0.566
1.931ProMet: 1.931 ± 0.309
2.18ProAsn: 2.18 ± 0.477
2.491ProPro: 2.491 ± 0.484
2.865ProGln: 2.865 ± 1.003
4.36ProArg: 4.36 ± 0.711
2.554ProSer: 2.554 ± 0.358
4.298ProThr: 4.298 ± 0.594
4.049ProVal: 4.049 ± 0.554
1.433ProTrp: 1.433 ± 0.399
1.806ProTyr: 1.806 ± 0.332
0.0ProXaa: 0.0 ± 0.0
Gln
4.235GlnAla: 4.235 ± 0.472
0.125GlnCys: 0.125 ± 0.089
1.806GlnAsp: 1.806 ± 0.342
1.931GlnGlu: 1.931 ± 0.381
1.495GlnPhe: 1.495 ± 0.352
4.36GlnGly: 4.36 ± 1.708
0.436GlnHis: 0.436 ± 0.164
2.118GlnIle: 2.118 ± 0.374
1.308GlnLys: 1.308 ± 0.433
2.99GlnLeu: 2.99 ± 0.531
0.747GlnMet: 0.747 ± 0.183
1.059GlnAsn: 1.059 ± 0.254
1.495GlnPro: 1.495 ± 0.311
1.308GlnGln: 1.308 ± 0.375
2.429GlnArg: 2.429 ± 0.399
1.619GlnSer: 1.619 ± 0.264
1.619GlnThr: 1.619 ± 0.289
2.616GlnVal: 2.616 ± 0.446
0.498GlnTrp: 0.498 ± 0.177
0.747GlnTyr: 0.747 ± 0.206
0.0GlnXaa: 0.0 ± 0.0
Arg
6.353ArgAla: 6.353 ± 0.667
0.81ArgCys: 0.81 ± 0.299
3.426ArgAsp: 3.426 ± 0.534
5.481ArgGlu: 5.481 ± 0.618
2.305ArgPhe: 2.305 ± 0.437
5.107ArgGly: 5.107 ± 0.659
1.433ArgHis: 1.433 ± 0.294
3.114ArgIle: 3.114 ± 0.378
4.235ArgLys: 4.235 ± 0.646
5.543ArgLeu: 5.543 ± 0.599
2.305ArgMet: 2.305 ± 0.318
2.429ArgAsn: 2.429 ± 0.34
3.239ArgPro: 3.239 ± 0.473
2.18ArgGln: 2.18 ± 0.42
5.419ArgArg: 5.419 ± 0.686
3.301ArgSer: 3.301 ± 0.398
3.114ArgThr: 3.114 ± 0.439
4.921ArgVal: 4.921 ± 0.601
1.682ArgTrp: 1.682 ± 0.359
2.242ArgTyr: 2.242 ± 0.435
0.0ArgXaa: 0.0 ± 0.0
Ser
5.606SerAla: 5.606 ± 0.641
0.062SerCys: 0.062 ± 0.059
3.675SerAsp: 3.675 ± 0.443
3.675SerGlu: 3.675 ± 0.514
1.993SerPhe: 1.993 ± 0.416
5.606SerGly: 5.606 ± 0.745
1.308SerHis: 1.308 ± 0.332
2.367SerIle: 2.367 ± 0.369
2.865SerLys: 2.865 ± 0.544
3.737SerLeu: 3.737 ± 0.478
1.619SerMet: 1.619 ± 0.324
1.557SerAsn: 1.557 ± 0.315
2.803SerPro: 2.803 ± 0.398
1.931SerGln: 1.931 ± 0.335
3.363SerArg: 3.363 ± 0.375
2.927SerSer: 2.927 ± 0.36
2.616SerThr: 2.616 ± 0.374
3.363SerVal: 3.363 ± 0.453
1.183SerTrp: 1.183 ± 0.28
1.246SerTyr: 1.246 ± 0.302
0.0SerXaa: 0.0 ± 0.0
Thr
5.045ThrAla: 5.045 ± 0.509
0.436ThrCys: 0.436 ± 0.167
3.114ThrAsp: 3.114 ± 0.465
4.235ThrGlu: 4.235 ± 0.505
2.18ThrPhe: 2.18 ± 0.424
5.419ThrGly: 5.419 ± 0.643
1.121ThrHis: 1.121 ± 0.299
2.18ThrIle: 2.18 ± 0.386
2.429ThrLys: 2.429 ± 0.383
5.357ThrLeu: 5.357 ± 0.451
1.308ThrMet: 1.308 ± 0.272
0.934ThrAsn: 0.934 ± 0.352
3.55ThrPro: 3.55 ± 0.513
2.242ThrGln: 2.242 ± 0.398
2.99ThrArg: 2.99 ± 0.495
3.114ThrSer: 3.114 ± 0.526
3.675ThrThr: 3.675 ± 0.52
4.36ThrVal: 4.36 ± 0.504
1.059ThrTrp: 1.059 ± 0.281
1.806ThrTyr: 1.806 ± 0.311
0.0ThrXaa: 0.0 ± 0.0
Val
5.979ValAla: 5.979 ± 0.83
0.872ValCys: 0.872 ± 0.265
5.606ValAsp: 5.606 ± 0.583
4.921ValGlu: 4.921 ± 0.614
2.491ValPhe: 2.491 ± 0.409
4.921ValGly: 4.921 ± 0.613
1.121ValHis: 1.121 ± 0.245
3.986ValIle: 3.986 ± 0.433
3.363ValLys: 3.363 ± 0.481
5.73ValLeu: 5.73 ± 0.573
1.183ValMet: 1.183 ± 0.271
2.865ValAsn: 2.865 ± 0.374
4.111ValPro: 4.111 ± 0.648
2.242ValGln: 2.242 ± 0.355
4.485ValArg: 4.485 ± 0.506
4.983ValSer: 4.983 ± 0.519
3.426ValThr: 3.426 ± 0.426
5.294ValVal: 5.294 ± 0.59
1.37ValTrp: 1.37 ± 0.316
2.18ValTyr: 2.18 ± 0.423
0.0ValXaa: 0.0 ± 0.0
Trp
1.869TrpAla: 1.869 ± 0.337
0.311TrpCys: 0.311 ± 0.159
1.308TrpAsp: 1.308 ± 0.296
1.557TrpGlu: 1.557 ± 0.305
0.374TrpPhe: 0.374 ± 0.165
1.433TrpGly: 1.433 ± 0.277
0.436TrpHis: 0.436 ± 0.194
1.495TrpIle: 1.495 ± 0.299
0.623TrpLys: 0.623 ± 0.199
1.806TrpLeu: 1.806 ± 0.317
0.747TrpMet: 0.747 ± 0.184
0.747TrpAsn: 0.747 ± 0.188
0.81TrpPro: 0.81 ± 0.2
0.81TrpGln: 0.81 ± 0.216
0.934TrpArg: 0.934 ± 0.245
1.183TrpSer: 1.183 ± 0.221
1.433TrpThr: 1.433 ± 0.313
1.619TrpVal: 1.619 ± 0.294
0.436TrpTrp: 0.436 ± 0.159
0.498TrpTyr: 0.498 ± 0.175
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.305TyrAla: 2.305 ± 0.326
0.311TyrCys: 0.311 ± 0.141
2.242TyrAsp: 2.242 ± 0.339
1.433TyrGlu: 1.433 ± 0.33
0.81TyrPhe: 0.81 ± 0.204
2.367TyrGly: 2.367 ± 0.32
0.685TyrHis: 0.685 ± 0.222
1.121TyrIle: 1.121 ± 0.261
1.183TyrLys: 1.183 ± 0.312
2.803TyrLeu: 2.803 ± 0.399
0.685TyrMet: 0.685 ± 0.217
1.308TyrAsn: 1.308 ± 0.255
1.121TyrPro: 1.121 ± 0.268
1.059TyrGln: 1.059 ± 0.267
2.678TyrArg: 2.678 ± 0.402
1.433TyrSer: 1.433 ± 0.309
1.495TyrThr: 1.495 ± 0.315
2.118TyrVal: 2.118 ± 0.342
0.685TyrTrp: 0.685 ± 0.224
1.121TyrTyr: 1.121 ± 0.29
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 87 proteins (16056 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski