Amino acid dipepetide frequency for Salmonella phage SJ46

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.363AlaAla: 10.363 ± 0.716
0.907AlaCys: 0.907 ± 0.173
5.797AlaAsp: 5.797 ± 0.467
6.606AlaGlu: 6.606 ± 0.571
3.498AlaPhe: 3.498 ± 0.382
6.833AlaGly: 6.833 ± 0.526
1.49AlaHis: 1.49 ± 0.262
6.024AlaIle: 6.024 ± 0.547
5.343AlaLys: 5.343 ± 0.419
9.294AlaLeu: 9.294 ± 0.616
2.429AlaMet: 2.429 ± 0.284
4.048AlaAsn: 4.048 ± 0.352
3.271AlaPro: 3.271 ± 0.378
4.178AlaGln: 4.178 ± 0.325
5.732AlaArg: 5.732 ± 0.474
5.02AlaSer: 5.02 ± 0.387
5.732AlaThr: 5.732 ± 0.363
5.441AlaVal: 5.441 ± 0.406
1.36AlaTrp: 1.36 ± 0.228
2.817AlaTyr: 2.817 ± 0.259
0.0AlaXaa: 0.0 ± 0.0
Cys
0.648CysAla: 0.648 ± 0.148
0.227CysCys: 0.227 ± 0.082
0.551CysAsp: 0.551 ± 0.143
0.551CysGlu: 0.551 ± 0.131
0.421CysPhe: 0.421 ± 0.098
0.939CysGly: 0.939 ± 0.245
0.324CysHis: 0.324 ± 0.082
0.551CysIle: 0.551 ± 0.126
0.486CysLys: 0.486 ± 0.133
0.648CysLeu: 0.648 ± 0.149
0.162CysMet: 0.162 ± 0.068
0.324CysAsn: 0.324 ± 0.101
0.518CysPro: 0.518 ± 0.12
0.518CysGln: 0.518 ± 0.171
0.874CysArg: 0.874 ± 0.17
0.615CysSer: 0.615 ± 0.144
0.389CysThr: 0.389 ± 0.097
0.486CysVal: 0.486 ± 0.136
0.194CysTrp: 0.194 ± 0.092
0.453CysTyr: 0.453 ± 0.118
0.0CysXaa: 0.0 ± 0.0
Asp
5.505AspAla: 5.505 ± 0.407
0.712AspCys: 0.712 ± 0.171
4.242AspAsp: 4.242 ± 0.491
4.21AspGlu: 4.21 ± 0.519
2.364AspPhe: 2.364 ± 0.212
5.149AspGly: 5.149 ± 0.428
0.972AspHis: 0.972 ± 0.192
3.141AspIle: 3.141 ± 0.373
2.85AspLys: 2.85 ± 0.292
4.469AspLeu: 4.469 ± 0.392
2.04AspMet: 2.04 ± 0.334
2.817AspAsn: 2.817 ± 0.316
2.17AspPro: 2.17 ± 0.242
2.073AspGln: 2.073 ± 0.261
3.141AspArg: 3.141 ± 0.307
4.307AspSer: 4.307 ± 0.389
3.4AspThr: 3.4 ± 0.359
4.145AspVal: 4.145 ± 0.415
0.81AspTrp: 0.81 ± 0.142
2.105AspTyr: 2.105 ± 0.247
0.0AspXaa: 0.0 ± 0.0
Glu
5.829GluAla: 5.829 ± 0.453
0.453GluCys: 0.453 ± 0.104
3.368GluAsp: 3.368 ± 0.405
4.242GluGlu: 4.242 ± 0.374
2.882GluPhe: 2.882 ± 0.287
3.919GluGly: 3.919 ± 0.358
1.425GluHis: 1.425 ± 0.256
4.372GluIle: 4.372 ± 0.369
4.016GluLys: 4.016 ± 0.531
7.416GluLeu: 7.416 ± 0.531
2.461GluMet: 2.461 ± 0.286
2.688GluAsn: 2.688 ± 0.351
2.396GluPro: 2.396 ± 0.306
3.206GluGln: 3.206 ± 0.38
4.275GluArg: 4.275 ± 0.535
3.757GluSer: 3.757 ± 0.439
3.174GluThr: 3.174 ± 0.299
3.659GluVal: 3.659 ± 0.314
0.972GluTrp: 0.972 ± 0.183
2.202GluTyr: 2.202 ± 0.286
0.0GluXaa: 0.0 ± 0.0
Phe
2.688PheAla: 2.688 ± 0.279
0.453PheCys: 0.453 ± 0.135
2.623PheAsp: 2.623 ± 0.253
2.105PheGlu: 2.105 ± 0.239
1.554PhePhe: 1.554 ± 0.219
2.299PheGly: 2.299 ± 0.26
0.453PheHis: 0.453 ± 0.11
2.429PheIle: 2.429 ± 0.27
1.684PheLys: 1.684 ± 0.237
2.72PheLeu: 2.72 ± 0.282
0.777PheMet: 0.777 ± 0.142
2.267PheAsn: 2.267 ± 0.239
1.425PhePro: 1.425 ± 0.236
1.328PheGln: 1.328 ± 0.208
2.04PheArg: 2.04 ± 0.219
2.591PheSer: 2.591 ± 0.294
2.04PheThr: 2.04 ± 0.261
2.105PheVal: 2.105 ± 0.302
0.518PheTrp: 0.518 ± 0.124
0.972PheTyr: 0.972 ± 0.189
0.0PheXaa: 0.0 ± 0.0
Gly
5.214GlyAla: 5.214 ± 0.419
0.712GlyCys: 0.712 ± 0.211
4.89GlyAsp: 4.89 ± 0.506
4.307GlyGlu: 4.307 ± 0.338
2.623GlyPhe: 2.623 ± 0.234
5.376GlyGly: 5.376 ± 0.479
1.166GlyHis: 1.166 ± 0.177
4.048GlyIle: 4.048 ± 0.438
4.955GlyLys: 4.955 ± 0.396
5.376GlyLeu: 5.376 ± 0.38
1.781GlyMet: 1.781 ± 0.214
3.206GlyAsn: 3.206 ± 0.412
1.069GlyPro: 1.069 ± 0.188
2.785GlyGln: 2.785 ± 0.259
4.307GlyArg: 4.307 ± 0.455
3.692GlySer: 3.692 ± 0.341
3.951GlyThr: 3.951 ± 0.388
5.052GlyVal: 5.052 ± 0.404
1.36GlyTrp: 1.36 ± 0.246
2.267GlyTyr: 2.267 ± 0.235
0.0GlyXaa: 0.0 ± 0.0
His
1.554HisAla: 1.554 ± 0.226
0.324HisCys: 0.324 ± 0.113
0.777HisAsp: 0.777 ± 0.156
0.907HisGlu: 0.907 ± 0.162
0.551HisPhe: 0.551 ± 0.117
1.231HisGly: 1.231 ± 0.165
0.486HisHis: 0.486 ± 0.127
1.004HisIle: 1.004 ± 0.229
1.004HisLys: 1.004 ± 0.195
1.554HisLeu: 1.554 ± 0.221
0.291HisMet: 0.291 ± 0.101
0.68HisAsn: 0.68 ± 0.124
0.939HisPro: 0.939 ± 0.182
0.874HisGln: 0.874 ± 0.17
1.393HisArg: 1.393 ± 0.233
1.133HisSer: 1.133 ± 0.179
0.842HisThr: 0.842 ± 0.139
1.069HisVal: 1.069 ± 0.21
0.324HisTrp: 0.324 ± 0.126
0.518HisTyr: 0.518 ± 0.146
0.0HisXaa: 0.0 ± 0.0
Ile
6.088IleAla: 6.088 ± 0.438
0.648IleCys: 0.648 ± 0.122
3.659IleAsp: 3.659 ± 0.324
4.501IleGlu: 4.501 ± 0.331
1.749IlePhe: 1.749 ± 0.277
3.562IleGly: 3.562 ± 0.359
0.907IleHis: 0.907 ± 0.15
2.915IleIle: 2.915 ± 0.328
3.336IleLys: 3.336 ± 0.34
4.21IleLeu: 4.21 ± 0.399
1.133IleMet: 1.133 ± 0.233
3.206IleAsn: 3.206 ± 0.323
2.558IlePro: 2.558 ± 0.28
2.008IleGln: 2.008 ± 0.268
3.271IleArg: 3.271 ± 0.315
4.761IleSer: 4.761 ± 0.376
4.307IleThr: 4.307 ± 0.333
3.012IleVal: 3.012 ± 0.323
0.453IleTrp: 0.453 ± 0.102
1.587IleTyr: 1.587 ± 0.221
0.0IleXaa: 0.0 ± 0.0
Lys
6.218LysAla: 6.218 ± 0.603
0.356LysCys: 0.356 ± 0.119
3.044LysAsp: 3.044 ± 0.301
4.404LysGlu: 4.404 ± 0.442
1.587LysPhe: 1.587 ± 0.23
3.465LysGly: 3.465 ± 0.355
1.198LysHis: 1.198 ± 0.189
3.206LysIle: 3.206 ± 0.38
4.21LysLys: 4.21 ± 0.458
4.793LysLeu: 4.793 ± 0.478
1.425LysMet: 1.425 ± 0.205
2.526LysAsn: 2.526 ± 0.273
2.235LysPro: 2.235 ± 0.301
2.429LysGln: 2.429 ± 0.28
2.688LysArg: 2.688 ± 0.296
4.016LysSer: 4.016 ± 0.368
3.433LysThr: 3.433 ± 0.318
3.368LysVal: 3.368 ± 0.398
0.777LysTrp: 0.777 ± 0.159
2.17LysTyr: 2.17 ± 0.315
0.0LysXaa: 0.0 ± 0.0
Leu
9.23LeuAla: 9.23 ± 0.61
0.939LeuCys: 0.939 ± 0.198
5.343LeuAsp: 5.343 ± 0.316
6.088LeuGlu: 6.088 ± 0.508
2.558LeuPhe: 2.558 ± 0.238
5.084LeuGly: 5.084 ± 0.514
1.522LeuHis: 1.522 ± 0.213
4.34LeuIle: 4.34 ± 0.339
6.056LeuLys: 6.056 ± 0.523
6.833LeuLeu: 6.833 ± 0.676
2.558LeuMet: 2.558 ± 0.278
4.016LeuAsn: 4.016 ± 0.318
4.178LeuPro: 4.178 ± 0.371
3.595LeuGln: 3.595 ± 0.344
5.246LeuArg: 5.246 ± 0.573
6.801LeuSer: 6.801 ± 0.455
4.89LeuThr: 4.89 ± 0.385
5.732LeuVal: 5.732 ± 0.45
0.874LeuTrp: 0.874 ± 0.181
2.202LeuTyr: 2.202 ± 0.224
0.0LeuXaa: 0.0 ± 0.0
Met
2.85MetAla: 2.85 ± 0.345
0.291MetCys: 0.291 ± 0.101
1.393MetAsp: 1.393 ± 0.24
1.231MetGlu: 1.231 ± 0.216
0.68MetPhe: 0.68 ± 0.136
1.393MetGly: 1.393 ± 0.23
0.356MetHis: 0.356 ± 0.114
1.457MetIle: 1.457 ± 0.207
1.684MetLys: 1.684 ± 0.215
2.429MetLeu: 2.429 ± 0.322
0.81MetMet: 0.81 ± 0.184
1.295MetAsn: 1.295 ± 0.193
1.263MetPro: 1.263 ± 0.217
1.166MetGln: 1.166 ± 0.169
1.781MetArg: 1.781 ± 0.24
1.911MetSer: 1.911 ± 0.192
1.684MetThr: 1.684 ± 0.198
1.069MetVal: 1.069 ± 0.162
0.259MetTrp: 0.259 ± 0.093
0.421MetTyr: 0.421 ± 0.15
0.0MetXaa: 0.0 ± 0.0
Asn
4.437AsnAla: 4.437 ± 0.409
0.259AsnCys: 0.259 ± 0.119
2.656AsnAsp: 2.656 ± 0.353
3.238AsnGlu: 3.238 ± 0.333
1.036AsnPhe: 1.036 ± 0.208
4.21AsnGly: 4.21 ± 0.418
0.842AsnHis: 0.842 ± 0.19
2.915AsnIle: 2.915 ± 0.234
2.623AsnLys: 2.623 ± 0.272
3.789AsnLeu: 3.789 ± 0.309
0.745AsnMet: 0.745 ± 0.165
2.623AsnAsn: 2.623 ± 0.466
1.846AsnPro: 1.846 ± 0.277
1.846AsnGln: 1.846 ± 0.26
2.656AsnArg: 2.656 ± 0.336
2.882AsnSer: 2.882 ± 0.24
2.461AsnThr: 2.461 ± 0.318
2.656AsnVal: 2.656 ± 0.27
0.551AsnTrp: 0.551 ± 0.118
1.554AsnTyr: 1.554 ± 0.212
0.0AsnXaa: 0.0 ± 0.0
Pro
3.724ProAla: 3.724 ± 0.333
0.324ProCys: 0.324 ± 0.11
3.044ProAsp: 3.044 ± 0.335
3.951ProGlu: 3.951 ± 0.307
1.522ProPhe: 1.522 ± 0.201
2.979ProGly: 2.979 ± 0.314
0.518ProHis: 0.518 ± 0.154
1.781ProIle: 1.781 ± 0.258
1.749ProLys: 1.749 ± 0.236
3.109ProLeu: 3.109 ± 0.375
0.648ProMet: 0.648 ± 0.122
1.587ProAsn: 1.587 ± 0.206
1.457ProPro: 1.457 ± 0.187
1.652ProGln: 1.652 ± 0.228
1.619ProArg: 1.619 ± 0.274
2.299ProSer: 2.299 ± 0.247
2.396ProThr: 2.396 ± 0.257
3.595ProVal: 3.595 ± 0.345
0.486ProTrp: 0.486 ± 0.153
1.522ProTyr: 1.522 ± 0.189
0.0ProXaa: 0.0 ± 0.0
Gln
3.659GlnAla: 3.659 ± 0.374
0.291GlnCys: 0.291 ± 0.095
1.781GlnAsp: 1.781 ± 0.251
2.623GlnGlu: 2.623 ± 0.264
1.522GlnPhe: 1.522 ± 0.282
1.975GlnGly: 1.975 ± 0.252
1.069GlnHis: 1.069 ± 0.165
2.17GlnIle: 2.17 ± 0.309
2.526GlnLys: 2.526 ± 0.328
4.08GlnLeu: 4.08 ± 0.34
1.36GlnMet: 1.36 ± 0.182
2.073GlnAsn: 2.073 ± 0.218
1.393GlnPro: 1.393 ± 0.191
2.591GlnGln: 2.591 ± 0.278
2.882GlnArg: 2.882 ± 0.313
2.623GlnSer: 2.623 ± 0.306
2.008GlnThr: 2.008 ± 0.264
2.364GlnVal: 2.364 ± 0.24
0.583GlnTrp: 0.583 ± 0.155
1.781GlnTyr: 1.781 ± 0.261
0.0GlnXaa: 0.0 ± 0.0
Arg
6.056ArgAla: 6.056 ± 0.491
0.518ArgCys: 0.518 ± 0.113
3.336ArgAsp: 3.336 ± 0.327
3.951ArgGlu: 3.951 ± 0.41
2.17ArgPhe: 2.17 ± 0.229
3.271ArgGly: 3.271 ± 0.357
1.004ArgHis: 1.004 ± 0.226
4.113ArgIle: 4.113 ± 0.397
4.34ArgLys: 4.34 ± 0.406
5.57ArgLeu: 5.57 ± 0.413
1.652ArgMet: 1.652 ± 0.229
2.461ArgAsn: 2.461 ± 0.261
2.267ArgPro: 2.267 ± 0.252
2.04ArgGln: 2.04 ± 0.284
4.922ArgArg: 4.922 ± 0.526
2.979ArgSer: 2.979 ± 0.318
2.947ArgThr: 2.947 ± 0.314
3.303ArgVal: 3.303 ± 0.31
0.874ArgTrp: 0.874 ± 0.179
2.137ArgTyr: 2.137 ± 0.292
0.0ArgXaa: 0.0 ± 0.0
Ser
5.894SerAla: 5.894 ± 0.444
0.81SerCys: 0.81 ± 0.154
4.404SerAsp: 4.404 ± 0.33
3.498SerGlu: 3.498 ± 0.294
2.073SerPhe: 2.073 ± 0.292
5.635SerGly: 5.635 ± 0.417
0.777SerHis: 0.777 ± 0.15
4.307SerIle: 4.307 ± 0.305
3.433SerLys: 3.433 ± 0.387
6.25SerLeu: 6.25 ± 0.447
1.587SerMet: 1.587 ± 0.269
2.494SerAsn: 2.494 ± 0.323
2.364SerPro: 2.364 ± 0.301
2.558SerGln: 2.558 ± 0.301
3.465SerArg: 3.465 ± 0.231
4.21SerSer: 4.21 ± 0.429
3.498SerThr: 3.498 ± 0.36
5.117SerVal: 5.117 ± 0.371
0.874SerTrp: 0.874 ± 0.163
1.652SerTyr: 1.652 ± 0.297
0.0SerXaa: 0.0 ± 0.0
Thr
6.088ThrAla: 6.088 ± 0.467
0.389ThrCys: 0.389 ± 0.105
3.336ThrAsp: 3.336 ± 0.304
3.53ThrGlu: 3.53 ± 0.267
2.332ThrPhe: 2.332 ± 0.274
4.566ThrGly: 4.566 ± 0.403
1.101ThrHis: 1.101 ± 0.174
3.498ThrIle: 3.498 ± 0.316
2.235ThrLys: 2.235 ± 0.264
5.441ThrLeu: 5.441 ± 0.421
1.101ThrMet: 1.101 ± 0.176
2.137ThrAsn: 2.137 ± 0.32
3.53ThrPro: 3.53 ± 0.274
1.975ThrGln: 1.975 ± 0.264
2.72ThrArg: 2.72 ± 0.284
3.789ThrSer: 3.789 ± 0.397
3.206ThrThr: 3.206 ± 0.41
3.692ThrVal: 3.692 ± 0.372
0.648ThrTrp: 0.648 ± 0.168
2.073ThrTyr: 2.073 ± 0.287
0.0ThrXaa: 0.0 ± 0.0
Val
6.056ValAla: 6.056 ± 0.411
0.648ValCys: 0.648 ± 0.134
3.983ValAsp: 3.983 ± 0.364
3.789ValGlu: 3.789 ± 0.344
2.073ValPhe: 2.073 ± 0.231
3.271ValGly: 3.271 ± 0.304
0.777ValHis: 0.777 ± 0.172
3.044ValIle: 3.044 ± 0.299
3.303ValLys: 3.303 ± 0.283
5.797ValLeu: 5.797 ± 0.441
1.393ValMet: 1.393 ± 0.215
3.368ValAsn: 3.368 ± 0.351
2.817ValPro: 2.817 ± 0.296
2.461ValGln: 2.461 ± 0.304
3.659ValArg: 3.659 ± 0.35
4.955ValSer: 4.955 ± 0.433
4.631ValThr: 4.631 ± 0.367
3.854ValVal: 3.854 ± 0.429
1.101ValTrp: 1.101 ± 0.196
1.749ValTyr: 1.749 ± 0.282
0.0ValXaa: 0.0 ± 0.0
Trp
1.263TrpAla: 1.263 ± 0.195
0.194TrpCys: 0.194 ± 0.086
0.68TrpAsp: 0.68 ± 0.146
0.745TrpGlu: 0.745 ± 0.133
0.712TrpPhe: 0.712 ± 0.152
0.745TrpGly: 0.745 ± 0.173
0.421TrpHis: 0.421 ± 0.098
0.842TrpIle: 0.842 ± 0.2
0.551TrpLys: 0.551 ± 0.121
1.781TrpLeu: 1.781 ± 0.339
0.227TrpMet: 0.227 ± 0.089
0.712TrpAsn: 0.712 ± 0.181
0.68TrpPro: 0.68 ± 0.178
0.583TrpGln: 0.583 ± 0.129
0.874TrpArg: 0.874 ± 0.187
0.551TrpSer: 0.551 ± 0.143
0.518TrpThr: 0.518 ± 0.134
0.939TrpVal: 0.939 ± 0.152
0.324TrpTrp: 0.324 ± 0.114
0.324TrpTyr: 0.324 ± 0.104
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.85TyrAla: 2.85 ± 0.261
0.421TyrCys: 0.421 ± 0.116
1.716TyrAsp: 1.716 ± 0.238
2.235TyrGlu: 2.235 ± 0.244
1.166TyrPhe: 1.166 ± 0.189
2.235TyrGly: 2.235 ± 0.314
0.648TyrHis: 0.648 ± 0.136
1.716TyrIle: 1.716 ± 0.237
1.166TyrLys: 1.166 ± 0.163
2.526TyrLeu: 2.526 ± 0.244
0.81TyrMet: 0.81 ± 0.16
1.295TyrAsn: 1.295 ± 0.18
1.587TyrPro: 1.587 ± 0.21
1.49TyrGln: 1.49 ± 0.198
2.364TyrArg: 2.364 ± 0.287
2.008TyrSer: 2.008 ± 0.234
1.878TyrThr: 1.878 ± 0.251
2.073TyrVal: 2.073 ± 0.29
0.324TyrTrp: 0.324 ± 0.088
0.777TyrTyr: 0.777 ± 0.176
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 122 proteins (30880 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski