Zymoseptoria tritici ST99CH_3D7

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; dothideomyceta; Dothideomycetes; Dothideomycetidae; Mycosphaerellales; Mycosphaerellaceae; Zymoseptoria; Zymoseptoria tritici

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11722 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X7RT76|A0A1X7RT76_ZYMTR AA_permease domain-containing protein OS=Zymoseptoria tritici ST99CH_3D7 OX=1276538 GN=ZT3D7_G5785 PE=4 SV=1
MM1 pKa = 7.36GLAASSVSFEE11 pKa = 4.17SMDD14 pKa = 3.12VGGAVLLQDD23 pKa = 3.27GVVPVFLTSYY33 pKa = 10.39GFSPANMLSFGVIAALSYY51 pKa = 9.33FSPAFFFGVTDD62 pKa = 3.25TDD64 pKa = 4.37YY65 pKa = 11.56IDD67 pKa = 3.76VQPVLTFPVIDD78 pKa = 3.99AGGAASTSVVTCAPFNNGMLPPPEE102 pKa = 4.29SSGVDD107 pKa = 3.03PVVYY111 pKa = 9.27ITATNQLKK119 pKa = 10.62CSIPNTSIAPGVVTIAFEE137 pKa = 4.12KK138 pKa = 10.58SDD140 pKa = 4.13GIFFTDD146 pKa = 3.34ASYY149 pKa = 11.57GFLVADD155 pKa = 3.72STILADD161 pKa = 3.86TVTATNTVFVPGSTVGSTTVTSTRR185 pKa = 11.84YY186 pKa = 9.89IFTDD190 pKa = 3.27EE191 pKa = 4.19ATSTSTTTLSDD202 pKa = 3.39ASSTTYY208 pKa = 10.48ISCSTSTSATNTGTATADD226 pKa = 3.72PNSTSATDD234 pKa = 3.42SSSITSATDD243 pKa = 3.72LSSTTSATDD252 pKa = 3.13SSNTSSSATDD262 pKa = 3.56DD263 pKa = 3.63SSSITISDD271 pKa = 3.78TPTAPTYY278 pKa = 8.26PTDD281 pKa = 3.84SNPFSSSTSCTNPQTPSPTPQKK303 pKa = 10.48NIKK306 pKa = 8.62TVFITDD312 pKa = 3.5CAKK315 pKa = 10.51AAKK318 pKa = 9.25TSAAAAALSARR329 pKa = 11.84QTTATDD335 pKa = 3.44TLASNFGAPDD345 pKa = 3.56FTFSNGDD352 pKa = 3.07NTATITVTSTAIDD365 pKa = 3.49TRR367 pKa = 11.84STVVTSGTDD376 pKa = 3.17TSVVTEE382 pKa = 4.31SDD384 pKa = 3.26PTTTRR389 pKa = 11.84TVTAATVDD397 pKa = 3.42EE398 pKa = 4.55RR399 pKa = 11.84TVEE402 pKa = 4.23TVVGCEE408 pKa = 3.81GSEE411 pKa = 4.08TARR414 pKa = 3.84

Molecular weight: 41.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X7RQL8|A0A1X7RQL8_ZYMTR NADH-cytochrome b5 reductase OS=Zymoseptoria tritici ST99CH_3D7 OX=1276538 GN=ZT3D7_G4895 PE=3 SV=1
MM1 pKa = 7.59SYY3 pKa = 10.56LARR6 pKa = 11.84RR7 pKa = 11.84AAATVRR13 pKa = 11.84LANTITTRR21 pKa = 11.84RR22 pKa = 11.84GLVGISAGRR31 pKa = 11.84AATIVRR37 pKa = 11.84LTSNAPGRR45 pKa = 11.84IHH47 pKa = 6.96ARR49 pKa = 11.84STARR53 pKa = 11.84RR54 pKa = 11.84AVPGVRR60 pKa = 11.84LVNTAWRR67 pKa = 11.84PDD69 pKa = 3.3MPAQGSSIAA78 pKa = 3.92

Molecular weight: 8.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11722

0

11722

5360897

66

6000

457.3

50.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.154 ± 0.021

1.21 ± 0.009

5.847 ± 0.019

6.347 ± 0.024

3.532 ± 0.014

7.047 ± 0.023

2.396 ± 0.01

4.533 ± 0.013

4.903 ± 0.021

8.565 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.236 ± 0.009

3.589 ± 0.012

6.029 ± 0.024

4.101 ± 0.017

6.169 ± 0.023

8.117 ± 0.029

6.154 ± 0.022

6.036 ± 0.014

1.412 ± 0.009

2.622 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski