Mahlapitsi orthoreovirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Spinareovirinae; Orthoreovirus

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G1HJT8|A0A3G1HJT8_9REOV Mu B OS=Mahlapitsi orthoreovirus OX=2170064 PE=4 SV=1
MM1 pKa = 6.92GTASSLVQNFNIYY14 pKa = 11.06GNGNVFRR21 pKa = 11.84PTADD25 pKa = 3.15QVSTAVPTLSLQPGVLNPGGLAWRR49 pKa = 11.84RR50 pKa = 11.84INPDD54 pKa = 2.78VDD56 pKa = 3.53PTAPGALIQMTTDD69 pKa = 5.27DD70 pKa = 4.89IPTASLTCTEE80 pKa = 4.28NASGLVPVNAMYY92 pKa = 10.67MMLKK96 pKa = 10.02KK97 pKa = 10.64EE98 pKa = 4.08EE99 pKa = 4.18LVVVTDD105 pKa = 3.63HH106 pKa = 7.7AIDD109 pKa = 3.54QMHH112 pKa = 6.57KK113 pKa = 10.67LEE115 pKa = 4.18YY116 pKa = 11.04AMEE119 pKa = 4.66LSRR122 pKa = 11.84AYY124 pKa = 10.87LNEE127 pKa = 3.8KK128 pKa = 10.38SILPQSTSFDD138 pKa = 3.72SYY140 pKa = 10.97VIRR143 pKa = 11.84VDD145 pKa = 3.9CYY147 pKa = 9.95VGASALQAARR157 pKa = 11.84NFVRR161 pKa = 11.84GAPLITRR168 pKa = 11.84SRR170 pKa = 11.84MVAYY174 pKa = 7.45MTAIQNAFEE183 pKa = 4.83ILSDD187 pKa = 3.51WEE189 pKa = 4.07SDD191 pKa = 2.7IRR193 pKa = 11.84EE194 pKa = 4.25AMNLIPSTTPYY205 pKa = 11.08GATTCDD211 pKa = 3.33MQSVIKK217 pKa = 10.39FLDD220 pKa = 3.44EE221 pKa = 4.11NLPEE225 pKa = 4.29NSLVRR230 pKa = 11.84LYY232 pKa = 9.55PQQAADD238 pKa = 3.64SIARR242 pKa = 11.84RR243 pKa = 11.84NGGIRR248 pKa = 11.84WKK250 pKa = 11.01DD251 pKa = 3.57EE252 pKa = 3.77VTGDD256 pKa = 3.81VPSLATNTVAAATLGVINNTVPLGEE281 pKa = 4.56KK282 pKa = 10.32SDD284 pKa = 3.84VTSEE288 pKa = 3.81AMSLVTVVKK297 pKa = 9.88PDD299 pKa = 3.55LVTSMRR305 pKa = 11.84PVSSSVFATTVSPKK319 pKa = 9.46TYY321 pKa = 10.2NIRR324 pKa = 11.84AQPVMEE330 pKa = 4.53ALWLRR335 pKa = 11.84TAAAGVDD342 pKa = 3.79MVVGWWSEE350 pKa = 3.78EE351 pKa = 3.81HH352 pKa = 6.73AATHH356 pKa = 5.05YY357 pKa = 9.83TVIRR361 pKa = 11.84PGTKK365 pKa = 9.92VINLQQTGSMLITVDD380 pKa = 5.74LVDD383 pKa = 3.29KK384 pKa = 10.58DD385 pKa = 3.48YY386 pKa = 10.62RR387 pKa = 11.84TVHH390 pKa = 6.36FNPDD394 pKa = 3.25GQTVILLLLQSKK406 pKa = 10.33IPFEE410 pKa = 4.11QLTSPTEE417 pKa = 4.38LIGVAQVASVVLSASDD433 pKa = 3.42SSTEE437 pKa = 3.67GSAIINNTNLSYY449 pKa = 10.97LFEE452 pKa = 5.4RR453 pKa = 11.84EE454 pKa = 3.89ILRR457 pKa = 11.84GPDD460 pKa = 3.45TEE462 pKa = 4.42VNTYY466 pKa = 9.45LLCGFRR472 pKa = 11.84TDD474 pKa = 3.78SQPTKK479 pKa = 11.09EE480 pKa = 4.01NMPWYY485 pKa = 9.58DD486 pKa = 2.9AWDD489 pKa = 3.72AKK491 pKa = 8.49TTLTPLTTGEE501 pKa = 4.17VTLNGAAVPFVTPMSLIGAYY521 pKa = 8.56TPEE524 pKa = 4.15AMQGALPNDD533 pKa = 3.63AGILLAEE540 pKa = 4.3RR541 pKa = 11.84AMNLAEE547 pKa = 4.85AIKK550 pKa = 11.07LEE552 pKa = 4.61DD553 pKa = 4.7DD554 pKa = 3.87SMADD558 pKa = 3.32EE559 pKa = 5.2ASPFSAPIQGVLAIQQHH576 pKa = 5.32EE577 pKa = 4.18PGEE580 pKa = 4.47GVKK583 pKa = 10.39AWLGPFDD590 pKa = 4.53ILRR593 pKa = 11.84KK594 pKa = 8.85AARR597 pKa = 11.84AIQVFLGNPKK607 pKa = 9.79SVLMQGVPVVSDD619 pKa = 3.89PNVWIAMVQGIRR631 pKa = 11.84DD632 pKa = 4.59GIRR635 pKa = 11.84NKK637 pKa = 10.41SLSVGVRR644 pKa = 11.84TALDD648 pKa = 3.4NLNAVQSVRR657 pKa = 11.84TWKK660 pKa = 10.56SDD662 pKa = 3.16FLTKK666 pKa = 10.22VEE668 pKa = 4.49KK669 pKa = 10.64YY670 pKa = 9.97YY671 pKa = 10.45PPSSEE676 pKa = 3.81

Molecular weight: 73.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G1HJT8|A0A3G1HJT8_9REOV Mu B OS=Mahlapitsi orthoreovirus OX=2170064 PE=4 SV=1
MM1 pKa = 7.64GSGPSNFVNHH11 pKa = 6.97ASGEE15 pKa = 4.21AIISGLSDD23 pKa = 2.93QTNRR27 pKa = 11.84LGSLLSQNVYY37 pKa = 10.35NIIYY41 pKa = 8.74FFVIGGLILSAGYY54 pKa = 10.54GLYY57 pKa = 10.1KK58 pKa = 9.18YY59 pKa = 10.27CKK61 pKa = 8.23YY62 pKa = 10.01KK63 pKa = 10.28QRR65 pKa = 11.84RR66 pKa = 11.84AKK68 pKa = 10.58NLTRR72 pKa = 11.84QLSRR76 pKa = 11.84EE77 pKa = 4.15LIDD80 pKa = 3.85LNRR83 pKa = 11.84KK84 pKa = 8.84IEE86 pKa = 4.62HH87 pKa = 6.47ISGGKK92 pKa = 9.28IPATKK97 pKa = 9.9PSAPRR102 pKa = 11.84YY103 pKa = 7.11TPPCYY108 pKa = 9.97KK109 pKa = 10.33EE110 pKa = 4.03PIYY113 pKa = 11.27NEE115 pKa = 3.82VCEE118 pKa = 4.35GGFYY122 pKa = 10.94GNCC125 pKa = 3.45

Molecular weight: 13.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

0

11

7411

125

1294

673.7

75.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.597 ± 0.374

1.336 ± 0.126

5.937 ± 0.211

4.723 ± 0.302

3.562 ± 0.304

4.844 ± 0.258

2.375 ± 0.212

5.667 ± 0.257

3.967 ± 0.377

9.702 ± 0.317

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.996 ± 0.162

5.101 ± 0.33

5.735 ± 0.195

4.089 ± 0.213

5.735 ± 0.244

6.99 ± 0.236

6.207 ± 0.27

7.786 ± 0.359

1.741 ± 0.162

3.913 ± 0.357
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski