Roseovarius spongiae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Roseovarius

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3730 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3A8B669|A0A3A8B669_9RHOB Isoleucine--tRNA ligase OS=Roseovarius spongiae OX=2320272 GN=ileS PE=3 SV=1
MM1 pKa = 7.8EE2 pKa = 5.72GPPMRR7 pKa = 11.84YY8 pKa = 9.72SSILALMLLSSNLQAQDD25 pKa = 3.75LATLSLVTSEE35 pKa = 4.43EE36 pKa = 3.83SGAYY40 pKa = 8.92IVGPDD45 pKa = 3.73GRR47 pKa = 11.84PVYY50 pKa = 11.22AMLSADD56 pKa = 3.59SVGGDD61 pKa = 3.84GLDD64 pKa = 4.11SLDD67 pKa = 4.61SCAQSCRR74 pKa = 11.84DD75 pKa = 3.35NWPLLTAQEE84 pKa = 4.87DD85 pKa = 3.91ISVGEE90 pKa = 4.29GVNPDD95 pKa = 4.36LIGRR99 pKa = 11.84TASDD103 pKa = 3.64GQQVLTYY110 pKa = 10.5ADD112 pKa = 3.4QPLFQYY118 pKa = 10.04HH119 pKa = 5.38QDD121 pKa = 3.5VAGGEE126 pKa = 4.07PQGDD130 pKa = 4.33GIYY133 pKa = 10.78SFGGYY138 pKa = 6.62WALMAPSGRR147 pKa = 11.84PIRR150 pKa = 11.84SGTVPAADD158 pKa = 5.07NIPEE162 pKa = 4.29AQSGADD168 pKa = 3.66EE169 pKa = 4.73NEE171 pKa = 4.35SLPLPPVSKK180 pKa = 10.96

Molecular weight: 18.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3A8B2E0|A0A3A8B2E0_9RHOB Protease HtpX homolog OS=Roseovarius spongiae OX=2320272 GN=htpX PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3730

0

3730

1211542

19

11700

324.8

35.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.085 ± 0.056

0.899 ± 0.015

6.275 ± 0.048

6.008 ± 0.038

3.614 ± 0.024

8.976 ± 0.049

2.089 ± 0.021

5.008 ± 0.027

2.7 ± 0.027

10.026 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.769 ± 0.024

2.351 ± 0.021

5.209 ± 0.029

2.872 ± 0.02

7.505 ± 0.041

4.775 ± 0.024

5.181 ± 0.034

7.047 ± 0.031

1.425 ± 0.018

2.187 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski