Rhynchosporium commune

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes; Helotiales; Helotiales incertae sedis; Rhynchosporium

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12210 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1E1LCA8|A0A1E1LCA8_9HELO Related to potential drug facilitator PEP5 OS=Rhynchosporium commune OX=914237 GN=RCO7_09552 PE=4 SV=1
MM1 pKa = 7.64SKK3 pKa = 10.76LNPAWVYY10 pKa = 11.38LEE12 pKa = 3.86GHH14 pKa = 6.85PPATSTDD21 pKa = 3.34ATRR24 pKa = 11.84LLQMDD29 pKa = 3.8PTEE32 pKa = 4.49TSDD35 pKa = 4.63EE36 pKa = 4.4DD37 pKa = 3.77LTDD40 pKa = 3.34TSNSQRR46 pKa = 11.84PCSSVYY52 pKa = 10.29SDD54 pKa = 4.67HH55 pKa = 7.43FALQDD60 pKa = 3.36LVYY63 pKa = 10.63QVDD66 pKa = 3.91DD67 pKa = 4.63LLVDD71 pKa = 4.25GQLGTIEE78 pKa = 3.98QLEE81 pKa = 4.35MQNSILQEE89 pKa = 3.73EE90 pKa = 4.83VAQYY94 pKa = 10.56QRR96 pKa = 11.84TWDD99 pKa = 4.59AILEE103 pKa = 4.36MFDD106 pKa = 3.71EE107 pKa = 5.17VYY109 pKa = 9.63DD110 pKa = 3.79TALLLRR116 pKa = 11.84CSLEE120 pKa = 3.83EE121 pKa = 4.18CEE123 pKa = 4.5EE124 pKa = 4.79GISIAQADD132 pKa = 4.22WLALWGVRR140 pKa = 11.84DD141 pKa = 3.8ATEE144 pKa = 3.78VGNWII149 pKa = 4.02

Molecular weight: 16.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1E1LSG6|A0A1E1LSG6_9HELO Uncharacterized protein OS=Rhynchosporium commune OX=914237 GN=RCO7_00655 PE=4 SV=1
MM1 pKa = 7.1RR2 pKa = 11.84AKK4 pKa = 9.09WRR6 pKa = 11.84KK7 pKa = 8.87KK8 pKa = 8.73RR9 pKa = 11.84VRR11 pKa = 11.84RR12 pKa = 11.84LKK14 pKa = 9.95RR15 pKa = 11.84KK16 pKa = 8.83RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 7.63TRR21 pKa = 11.84ARR23 pKa = 11.84SS24 pKa = 3.18

Molecular weight: 3.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12210

0

12210

5554614

24

6762

454.9

50.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.202 ± 0.021

1.177 ± 0.008

5.546 ± 0.017

6.311 ± 0.021

3.753 ± 0.014

6.958 ± 0.02

2.246 ± 0.01

5.26 ± 0.014

5.375 ± 0.021

8.653 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.256 ± 0.008

3.906 ± 0.014

5.862 ± 0.021

3.893 ± 0.015

5.79 ± 0.02

8.648 ± 0.029

6.076 ± 0.015

5.98 ± 0.016

1.372 ± 0.008

2.736 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski