Bean leaf crumple virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 8.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y0B6D1|A0A1Y0B6D1_9GEMI Movement protein BC1 OS=Bean leaf crumple virus OX=2004460 GN=BC1 PE=3 SV=1
MM1 pKa = 7.76EE2 pKa = 4.81SQLVIPPNAFNYY14 pKa = 9.91IEE16 pKa = 4.27SQRR19 pKa = 11.84DD20 pKa = 3.77EE21 pKa = 4.43YY22 pKa = 11.22QLSHH26 pKa = 7.5DD27 pKa = 3.72LTEE30 pKa = 4.56IVLQFPSTAAQLSARR45 pKa = 11.84LTRR48 pKa = 11.84SCMKK52 pKa = 9.74IDD54 pKa = 3.33HH55 pKa = 6.45CVIEE59 pKa = 4.31YY60 pKa = 9.79RR61 pKa = 11.84QQVPINASGSVIVEE75 pKa = 3.74IHH77 pKa = 7.1DD78 pKa = 4.31KK79 pKa = 11.26RR80 pKa = 11.84MTDD83 pKa = 3.41NEE85 pKa = 4.36SLQASWTFPIRR96 pKa = 11.84CNIDD100 pKa = 2.83LHH102 pKa = 6.2YY103 pKa = 10.34FSSSFFSLKK112 pKa = 10.83DD113 pKa = 3.74PIPWKK118 pKa = 10.43LYY120 pKa = 10.92YY121 pKa = 9.85KK122 pKa = 10.3VCDD125 pKa = 4.04SNVHH129 pKa = 5.15QRR131 pKa = 11.84THH133 pKa = 5.39FAKK136 pKa = 10.85FKK138 pKa = 10.96GKK140 pKa = 10.52LKK142 pKa = 10.82LSTAKK147 pKa = 10.37HH148 pKa = 5.69SVDD151 pKa = 2.95IPFRR155 pKa = 11.84APTVKK160 pKa = 10.11ILSKK164 pKa = 10.67QFSDD168 pKa = 3.46KK169 pKa = 11.16DD170 pKa = 3.2IDD172 pKa = 3.91FHH174 pKa = 8.07HH175 pKa = 6.72VGYY178 pKa = 10.59GKK180 pKa = 9.39WEE182 pKa = 3.7RR183 pKa = 11.84RR184 pKa = 11.84MVRR187 pKa = 11.84SASSTRR193 pKa = 11.84FGLNGPIEE201 pKa = 4.13IRR203 pKa = 11.84PGEE206 pKa = 4.21SWASRR211 pKa = 11.84SVIGTAHH218 pKa = 6.55SNAEE222 pKa = 4.12SDD224 pKa = 3.69TTDD227 pKa = 2.77AFHH230 pKa = 8.01PYY232 pKa = 9.96RR233 pKa = 11.84DD234 pKa = 4.09LNRR237 pKa = 11.84LDD239 pKa = 4.19TPILDD244 pKa = 4.72PGDD247 pKa = 3.66SASIVGVQRR256 pKa = 11.84TQSNITMSLSQLNEE270 pKa = 3.95LVRR273 pKa = 11.84STVHH277 pKa = 5.77EE278 pKa = 4.81CINTNCIPLQPKK290 pKa = 8.84SLKK293 pKa = 10.51

Molecular weight: 33.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y0B6C6|A0A1Y0B6C6_9GEMI Replication enhancer OS=Bean leaf crumple virus OX=2004460 GN=AC3 PE=3 SV=1
MM1 pKa = 7.81KK2 pKa = 10.36LFKK5 pKa = 10.6CFNICPGRR13 pKa = 11.84SSNPRR18 pKa = 11.84TSEE21 pKa = 3.86SPEE24 pKa = 3.77RR25 pKa = 11.84NIPTGSRR32 pKa = 11.84TYY34 pKa = 9.44TALYY38 pKa = 9.16NYY40 pKa = 8.09PVSPTSRR47 pKa = 11.84MLDD50 pKa = 3.82FSTLLTPDD58 pKa = 4.14GLPVFTQTFRR68 pKa = 11.84QPKK71 pKa = 8.24TPMPSRR77 pKa = 11.84ITSPKK82 pKa = 9.71RR83 pKa = 11.84VIIVNPGNIKK93 pKa = 10.6CLGVPNQIKK102 pKa = 9.91KK103 pKa = 10.73SSIITPSMQEE113 pKa = 3.42VRR115 pKa = 11.84EE116 pKa = 4.04RR117 pKa = 11.84LSTLSRR123 pKa = 11.84SEE125 pKa = 3.57IQRR128 pKa = 11.84RR129 pKa = 11.84SS130 pKa = 3.09

Molecular weight: 14.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

1591

130

349

227.3

26.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.154 ± 0.883

1.948 ± 0.226

5.028 ± 0.649

4.148 ± 0.28

4.525 ± 0.265

5.091 ± 0.419

3.708 ± 0.457

6.411 ± 0.547

6.6 ± 0.434

6.6 ± 0.568

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.388 ± 0.502

5.783 ± 0.549

5.468 ± 0.728

3.708 ± 0.466

7.291 ± 0.871

9.177 ± 0.988

5.28 ± 0.666

6.097 ± 0.787

1.383 ± 0.224

4.211 ± 0.598
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski