Amino acid dipepetide frequency for Caulobacter phage Ccr34

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.753AlaAla: 14.753 ± 0.652
1.079AlaCys: 1.079 ± 0.12
7.425AlaAsp: 7.425 ± 0.364
7.312AlaGlu: 7.312 ± 0.467
4.606AlaPhe: 4.606 ± 0.247
9.068AlaGly: 9.068 ± 0.534
2.142AlaHis: 2.142 ± 0.192
5.379AlaIle: 5.379 ± 0.271
5.315AlaLys: 5.315 ± 0.388
10.598AlaLeu: 10.598 ± 0.559
3.527AlaMet: 3.527 ± 0.303
3.076AlaAsn: 3.076 ± 0.276
6.314AlaPro: 6.314 ± 0.35
4.381AlaGln: 4.381 ± 0.317
7.715AlaArg: 7.715 ± 0.444
5.814AlaSer: 5.814 ± 0.4
5.782AlaThr: 5.782 ± 0.437
7.538AlaVal: 7.538 ± 0.356
2.142AlaTrp: 2.142 ± 0.199
3.382AlaTyr: 3.382 ± 0.215
0.0AlaXaa: 0.0 ± 0.0
Cys
0.999CysAla: 0.999 ± 0.103
0.177CysCys: 0.177 ± 0.059
0.548CysAsp: 0.548 ± 0.094
0.644CysGlu: 0.644 ± 0.102
0.209CysPhe: 0.209 ± 0.052
0.66CysGly: 0.66 ± 0.106
0.242CysHis: 0.242 ± 0.071
0.29CysIle: 0.29 ± 0.086
0.274CysLys: 0.274 ± 0.069
0.757CysLeu: 0.757 ± 0.103
0.209CysMet: 0.209 ± 0.06
0.161CysAsn: 0.161 ± 0.047
0.387CysPro: 0.387 ± 0.08
0.37CysGln: 0.37 ± 0.077
0.918CysArg: 0.918 ± 0.12
0.37CysSer: 0.37 ± 0.079
0.435CysThr: 0.435 ± 0.089
0.757CysVal: 0.757 ± 0.108
0.161CysTrp: 0.161 ± 0.044
0.403CysTyr: 0.403 ± 0.082
0.0CysXaa: 0.0 ± 0.0
Asp
7.554AspAla: 7.554 ± 0.46
0.596AspCys: 0.596 ± 0.09
3.978AspAsp: 3.978 ± 0.374
4.945AspGlu: 4.945 ± 0.392
2.448AspPhe: 2.448 ± 0.203
6.008AspGly: 6.008 ± 0.327
1.433AspHis: 1.433 ± 0.186
3.189AspIle: 3.189 ± 0.221
2.351AspLys: 2.351 ± 0.224
6.539AspLeu: 6.539 ± 0.316
1.578AspMet: 1.578 ± 0.17
1.433AspAsn: 1.433 ± 0.155
4.123AspPro: 4.123 ± 0.323
1.723AspGln: 1.723 ± 0.15
4.075AspArg: 4.075 ± 0.265
1.981AspSer: 1.981 ± 0.176
2.915AspThr: 2.915 ± 0.285
4.735AspVal: 4.735 ± 0.296
1.305AspTrp: 1.305 ± 0.151
2.351AspTyr: 2.351 ± 0.199
0.0AspXaa: 0.0 ± 0.0
Glu
8.939GluAla: 8.939 ± 0.48
0.37GluCys: 0.37 ± 0.075
4.268GluAsp: 4.268 ± 0.353
4.171GluGlu: 4.171 ± 0.348
2.77GluPhe: 2.77 ± 0.249
4.316GluGly: 4.316 ± 0.257
1.578GluHis: 1.578 ± 0.187
3.511GluIle: 3.511 ± 0.259
2.835GluLys: 2.835 ± 0.217
3.559GluLeu: 3.559 ± 0.262
1.659GluMet: 1.659 ± 0.161
1.594GluAsn: 1.594 ± 0.19
2.98GluPro: 2.98 ± 0.237
2.029GluGln: 2.029 ± 0.195
4.896GluArg: 4.896 ± 0.394
2.126GluSer: 2.126 ± 0.197
4.316GluThr: 4.316 ± 0.242
4.494GluVal: 4.494 ± 0.244
0.821GluTrp: 0.821 ± 0.105
1.401GluTyr: 1.401 ± 0.199
0.0GluXaa: 0.0 ± 0.0
Phe
3.737PheAla: 3.737 ± 0.261
0.354PheCys: 0.354 ± 0.071
3.253PheAsp: 3.253 ± 0.244
2.174PheGlu: 2.174 ± 0.193
1.208PhePhe: 1.208 ± 0.142
2.448PheGly: 2.448 ± 0.155
1.208PheHis: 1.208 ± 0.149
1.562PheIle: 1.562 ± 0.163
1.836PheLys: 1.836 ± 0.194
3.028PheLeu: 3.028 ± 0.243
0.805PheMet: 0.805 ± 0.121
1.224PheAsn: 1.224 ± 0.141
1.611PhePro: 1.611 ± 0.153
1.176PheGln: 1.176 ± 0.123
2.19PheArg: 2.19 ± 0.187
2.158PheSer: 2.158 ± 0.218
2.078PheThr: 2.078 ± 0.224
2.593PheVal: 2.593 ± 0.206
0.676PheTrp: 0.676 ± 0.106
1.256PheTyr: 1.256 ± 0.152
0.0PheXaa: 0.0 ± 0.0
Gly
8.665GlyAla: 8.665 ± 0.63
0.515GlyCys: 0.515 ± 0.086
5.251GlyAsp: 5.251 ± 0.304
5.202GlyGlu: 5.202 ± 0.32
3.221GlyPhe: 3.221 ± 0.206
9.567GlyGly: 9.567 ± 2.015
1.756GlyHis: 1.756 ± 0.163
2.319GlyIle: 2.319 ± 0.177
3.994GlyLys: 3.994 ± 0.328
6.152GlyLeu: 6.152 ± 0.391
1.981GlyMet: 1.981 ± 0.163
2.094GlyAsn: 2.094 ± 0.321
2.899GlyPro: 2.899 ± 0.205
2.819GlyGln: 2.819 ± 0.216
5.54GlyArg: 5.54 ± 0.292
4.719GlySer: 4.719 ± 0.459
4.542GlyThr: 4.542 ± 0.556
5.846GlyVal: 5.846 ± 0.344
1.756GlyTrp: 1.756 ± 0.203
2.738GlyTyr: 2.738 ± 0.253
0.0GlyXaa: 0.0 ± 0.0
His
2.722HisAla: 2.722 ± 0.234
0.322HisCys: 0.322 ± 0.068
1.643HisAsp: 1.643 ± 0.153
1.401HisGlu: 1.401 ± 0.141
0.757HisPhe: 0.757 ± 0.127
2.19HisGly: 2.19 ± 0.21
0.451HisHis: 0.451 ± 0.091
0.693HisIle: 0.693 ± 0.107
0.644HisLys: 0.644 ± 0.117
1.836HisLeu: 1.836 ± 0.167
0.387HisMet: 0.387 ± 0.088
0.58HisAsn: 0.58 ± 0.09
1.208HisPro: 1.208 ± 0.148
0.628HisGln: 0.628 ± 0.112
1.514HisArg: 1.514 ± 0.19
0.789HisSer: 0.789 ± 0.118
0.982HisThr: 0.982 ± 0.132
1.417HisVal: 1.417 ± 0.155
0.515HisTrp: 0.515 ± 0.091
0.87HisTyr: 0.87 ± 0.117
0.0HisXaa: 0.0 ± 0.0
Ile
5.718IleAla: 5.718 ± 0.354
0.419IleCys: 0.419 ± 0.076
3.253IleAsp: 3.253 ± 0.219
3.865IleGlu: 3.865 ± 0.297
1.16IlePhe: 1.16 ± 0.149
3.527IleGly: 3.527 ± 0.293
1.16IleHis: 1.16 ± 0.166
2.19IleIle: 2.19 ± 0.197
2.239IleLys: 2.239 ± 0.228
3.527IleLeu: 3.527 ± 0.208
0.693IleMet: 0.693 ± 0.083
1.498IleAsn: 1.498 ± 0.176
2.4IlePro: 2.4 ± 0.193
1.514IleGln: 1.514 ± 0.186
2.915IleArg: 2.915 ± 0.207
2.029IleSer: 2.029 ± 0.173
2.996IleThr: 2.996 ± 0.244
3.35IleVal: 3.35 ± 0.227
0.564IleTrp: 0.564 ± 0.098
1.208IleTyr: 1.208 ± 0.129
0.0IleXaa: 0.0 ± 0.0
Lys
6.217LysAla: 6.217 ± 0.42
0.113LysCys: 0.113 ± 0.045
2.577LysAsp: 2.577 ± 0.235
1.997LysGlu: 1.997 ± 0.2
1.353LysPhe: 1.353 ± 0.132
2.819LysGly: 2.819 ± 0.267
1.047LysHis: 1.047 ± 0.137
2.126LysIle: 2.126 ± 0.169
2.319LysLys: 2.319 ± 0.219
3.801LysLeu: 3.801 ± 0.297
0.789LysMet: 0.789 ± 0.122
1.111LysAsn: 1.111 ± 0.122
2.738LysPro: 2.738 ± 0.24
1.079LysGln: 1.079 ± 0.129
3.543LysArg: 3.543 ± 0.238
2.448LysSer: 2.448 ± 0.203
3.189LysThr: 3.189 ± 0.256
3.624LysVal: 3.624 ± 0.236
0.725LysTrp: 0.725 ± 0.094
0.821LysTyr: 0.821 ± 0.121
0.0LysXaa: 0.0 ± 0.0
Leu
9.873LeuAla: 9.873 ± 0.45
0.789LeuCys: 0.789 ± 0.13
6.12LeuAsp: 6.12 ± 0.352
4.945LeuGlu: 4.945 ± 0.304
2.561LeuPhe: 2.561 ± 0.224
6.442LeuGly: 6.442 ± 0.459
1.868LeuHis: 1.868 ± 0.189
4.123LeuIle: 4.123 ± 0.259
4.204LeuLys: 4.204 ± 0.283
6.056LeuLeu: 6.056 ± 0.344
1.627LeuMet: 1.627 ± 0.169
2.754LeuAsn: 2.754 ± 0.216
4.381LeuPro: 4.381 ± 0.283
2.513LeuGln: 2.513 ± 0.201
5.734LeuArg: 5.734 ± 0.327
4.928LeuSer: 4.928 ± 0.358
5.363LeuThr: 5.363 ± 0.305
4.961LeuVal: 4.961 ± 0.292
1.353LeuTrp: 1.353 ± 0.145
2.19LeuTyr: 2.19 ± 0.195
0.0LeuXaa: 0.0 ± 0.0
Met
2.48MetAla: 2.48 ± 0.203
0.129MetCys: 0.129 ± 0.044
1.16MetAsp: 1.16 ± 0.135
0.982MetGlu: 0.982 ± 0.115
0.709MetPhe: 0.709 ± 0.121
1.53MetGly: 1.53 ± 0.177
0.387MetHis: 0.387 ± 0.087
1.433MetIle: 1.433 ± 0.151
0.982MetLys: 0.982 ± 0.148
1.82MetLeu: 1.82 ± 0.178
0.37MetMet: 0.37 ± 0.086
0.773MetAsn: 0.773 ± 0.114
1.353MetPro: 1.353 ± 0.175
0.628MetGln: 0.628 ± 0.102
1.739MetArg: 1.739 ± 0.18
1.836MetSer: 1.836 ± 0.188
2.432MetThr: 2.432 ± 0.21
1.385MetVal: 1.385 ± 0.171
0.225MetTrp: 0.225 ± 0.056
0.29MetTyr: 0.29 ± 0.06
0.0MetXaa: 0.0 ± 0.0
Asn
3.27AsnAla: 3.27 ± 0.285
0.29AsnCys: 0.29 ± 0.075
1.756AsnAsp: 1.756 ± 0.142
1.433AsnGlu: 1.433 ± 0.158
1.047AsnPhe: 1.047 ± 0.144
3.141AsnGly: 3.141 ± 0.295
0.564AsnHis: 0.564 ± 0.113
1.417AsnIle: 1.417 ± 0.138
1.16AsnLys: 1.16 ± 0.142
2.786AsnLeu: 2.786 ± 0.205
0.451AsnMet: 0.451 ± 0.092
1.144AsnAsn: 1.144 ± 0.183
2.029AsnPro: 2.029 ± 0.151
0.725AsnGln: 0.725 ± 0.122
1.868AsnArg: 1.868 ± 0.169
1.321AsnSer: 1.321 ± 0.169
1.53AsnThr: 1.53 ± 0.225
1.917AsnVal: 1.917 ± 0.224
0.499AsnTrp: 0.499 ± 0.097
0.773AsnTyr: 0.773 ± 0.133
0.0AsnXaa: 0.0 ± 0.0
Pro
6.104ProAla: 6.104 ± 0.32
0.435ProCys: 0.435 ± 0.093
3.592ProAsp: 3.592 ± 0.225
3.801ProGlu: 3.801 ± 0.262
2.078ProPhe: 2.078 ± 0.165
4.639ProGly: 4.639 ± 0.327
1.079ProHis: 1.079 ± 0.15
2.545ProIle: 2.545 ± 0.234
2.094ProLys: 2.094 ± 0.185
3.801ProLeu: 3.801 ± 0.255
1.224ProMet: 1.224 ± 0.148
1.772ProAsn: 1.772 ± 0.197
3.076ProPro: 3.076 ± 0.329
1.659ProGln: 1.659 ± 0.167
3.06ProArg: 3.06 ± 0.294
2.674ProSer: 2.674 ± 0.243
3.624ProThr: 3.624 ± 0.29
3.608ProVal: 3.608 ± 0.253
0.644ProTrp: 0.644 ± 0.1
1.611ProTyr: 1.611 ± 0.162
0.0ProXaa: 0.0 ± 0.0
Gln
4.349GlnAla: 4.349 ± 0.293
0.242GlnCys: 0.242 ± 0.061
1.981GlnAsp: 1.981 ± 0.167
2.126GlnGlu: 2.126 ± 0.184
1.16GlnPhe: 1.16 ± 0.123
2.464GlnGly: 2.464 ± 0.254
0.499GlnHis: 0.499 ± 0.087
1.643GlnIle: 1.643 ± 0.153
1.562GlnLys: 1.562 ± 0.15
1.723GlnLeu: 1.723 ± 0.207
0.805GlnMet: 0.805 ± 0.125
0.95GlnAsn: 0.95 ± 0.118
1.643GlnPro: 1.643 ± 0.16
1.16GlnGln: 1.16 ± 0.141
2.496GlnArg: 2.496 ± 0.234
1.305GlnSer: 1.305 ± 0.163
2.045GlnThr: 2.045 ± 0.182
2.271GlnVal: 2.271 ± 0.176
0.483GlnTrp: 0.483 ± 0.081
0.902GlnTyr: 0.902 ± 0.13
0.0GlnXaa: 0.0 ± 0.0
Arg
7.248ArgAla: 7.248 ± 0.375
0.838ArgCys: 0.838 ± 0.129
4.542ArgAsp: 4.542 ± 0.363
4.043ArgGlu: 4.043 ± 0.302
2.851ArgPhe: 2.851 ± 0.197
4.719ArgGly: 4.719 ± 0.3
1.578ArgHis: 1.578 ± 0.156
2.915ArgIle: 2.915 ± 0.213
3.076ArgLys: 3.076 ± 0.234
6.974ArgLeu: 6.974 ± 0.46
1.868ArgMet: 1.868 ± 0.187
2.045ArgAsn: 2.045 ± 0.152
3.398ArgPro: 3.398 ± 0.277
2.223ArgGln: 2.223 ± 0.187
6.233ArgArg: 6.233 ± 0.415
3.012ArgSer: 3.012 ± 0.307
3.334ArgThr: 3.334 ± 0.219
4.606ArgVal: 4.606 ± 0.282
1.514ArgTrp: 1.514 ± 0.142
2.802ArgTyr: 2.802 ± 0.198
0.0ArgXaa: 0.0 ± 0.0
Ser
5.186SerAla: 5.186 ± 0.313
0.499SerCys: 0.499 ± 0.085
2.754SerAsp: 2.754 ± 0.214
2.706SerGlu: 2.706 ± 0.228
1.981SerPhe: 1.981 ± 0.234
4.945SerGly: 4.945 ± 0.563
1.031SerHis: 1.031 ± 0.125
2.368SerIle: 2.368 ± 0.178
2.094SerLys: 2.094 ± 0.2
4.268SerLeu: 4.268 ± 0.272
1.385SerMet: 1.385 ± 0.176
1.369SerAsn: 1.369 ± 0.161
2.706SerPro: 2.706 ± 0.233
1.53SerGln: 1.53 ± 0.188
3.382SerArg: 3.382 ± 0.251
2.625SerSer: 2.625 ± 0.265
3.237SerThr: 3.237 ± 0.294
3.028SerVal: 3.028 ± 0.219
0.757SerTrp: 0.757 ± 0.1
1.739SerTyr: 1.739 ± 0.179
0.0SerXaa: 0.0 ± 0.0
Thr
7.215ThrAla: 7.215 ± 0.484
0.403ThrCys: 0.403 ± 0.088
3.414ThrAsp: 3.414 ± 0.307
3.237ThrGlu: 3.237 ± 0.189
2.4ThrPhe: 2.4 ± 0.285
5.009ThrGly: 5.009 ± 0.501
1.224ThrHis: 1.224 ± 0.137
3.189ThrIle: 3.189 ± 0.356
1.997ThrLys: 1.997 ± 0.185
5.637ThrLeu: 5.637 ± 0.31
1.208ThrMet: 1.208 ± 0.142
1.514ThrAsn: 1.514 ± 0.179
4.59ThrPro: 4.59 ± 0.31
1.981ThrGln: 1.981 ± 0.192
3.704ThrArg: 3.704 ± 0.224
2.851ThrSer: 2.851 ± 0.271
3.398ThrThr: 3.398 ± 0.371
4.107ThrVal: 4.107 ± 0.368
0.982ThrTrp: 0.982 ± 0.117
2.029ThrTyr: 2.029 ± 0.195
0.0ThrXaa: 0.0 ± 0.0
Val
7.328ValAla: 7.328 ± 0.301
0.886ValCys: 0.886 ± 0.137
4.526ValAsp: 4.526 ± 0.233
4.735ValGlu: 4.735 ± 0.296
2.448ValPhe: 2.448 ± 0.202
4.3ValGly: 4.3 ± 0.309
1.417ValHis: 1.417 ± 0.141
3.366ValIle: 3.366 ± 0.217
3.205ValLys: 3.205 ± 0.249
5.863ValLeu: 5.863 ± 0.295
1.369ValMet: 1.369 ± 0.146
2.255ValAsn: 2.255 ± 0.256
3.35ValPro: 3.35 ± 0.283
2.207ValGln: 2.207 ± 0.173
4.671ValArg: 4.671 ± 0.293
3.849ValSer: 3.849 ± 0.349
4.494ValThr: 4.494 ± 0.346
5.17ValVal: 5.17 ± 0.333
1.385ValTrp: 1.385 ± 0.15
2.142ValTyr: 2.142 ± 0.205
0.0ValXaa: 0.0 ± 0.0
Trp
1.643TrpAla: 1.643 ± 0.142
0.177TrpCys: 0.177 ± 0.049
1.192TrpAsp: 1.192 ± 0.124
0.982TrpGlu: 0.982 ± 0.122
0.757TrpPhe: 0.757 ± 0.126
1.176TrpGly: 1.176 ± 0.156
0.242TrpHis: 0.242 ± 0.057
0.676TrpIle: 0.676 ± 0.09
0.999TrpLys: 0.999 ± 0.142
1.611TrpLeu: 1.611 ± 0.17
0.322TrpMet: 0.322 ± 0.086
0.838TrpAsn: 0.838 ± 0.11
0.644TrpPro: 0.644 ± 0.104
0.403TrpGln: 0.403 ± 0.075
1.353TrpArg: 1.353 ± 0.147
1.127TrpSer: 1.127 ± 0.125
1.288TrpThr: 1.288 ± 0.148
1.144TrpVal: 1.144 ± 0.143
0.354TrpTrp: 0.354 ± 0.089
0.338TrpTyr: 0.338 ± 0.068
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.35TyrAla: 3.35 ± 0.203
0.338TyrCys: 0.338 ± 0.067
2.029TyrAsp: 2.029 ± 0.161
1.933TyrGlu: 1.933 ± 0.184
0.854TyrPhe: 0.854 ± 0.134
2.609TyrGly: 2.609 ± 0.209
0.644TyrHis: 0.644 ± 0.092
1.288TyrIle: 1.288 ± 0.156
1.256TyrLys: 1.256 ± 0.169
2.448TyrLeu: 2.448 ± 0.218
0.37TyrMet: 0.37 ± 0.066
0.934TyrAsn: 0.934 ± 0.142
1.288TyrPro: 1.288 ± 0.138
1.063TyrGln: 1.063 ± 0.137
2.271TyrArg: 2.271 ± 0.211
1.643TyrSer: 1.643 ± 0.167
2.11TyrThr: 2.11 ± 0.211
2.416TyrVal: 2.416 ± 0.21
0.403TyrTrp: 0.403 ± 0.078
0.966TyrTyr: 0.966 ± 0.126
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 321 proteins (62090 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski