Pseudohoeflea suaedae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Phyllobacteriaceae; Pseudohoeflea

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3469 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4V3A6X2|A0A4V3A6X2_9RHIZ Carbohydrate kinase OS=Pseudohoeflea suaedae OX=877384 GN=E2A64_14740 PE=3 SV=1
MM1 pKa = 7.67RR2 pKa = 11.84SVILFASFVLALVSSCFLLSSNSHH26 pKa = 6.57AAPFPCEE33 pKa = 3.8GNTISSGNEE42 pKa = 3.07IVVQLDD48 pKa = 3.53GAITCGTAYY57 pKa = 10.86NSTDD61 pKa = 3.51EE62 pKa = 5.6IEE64 pKa = 4.14ILNNGIVSGSTIQVLIAFYY83 pKa = 10.83NGATGSVTGGTTSDD97 pKa = 3.67DD98 pKa = 3.44SGTFLSPEE106 pKa = 4.16CNVTVGCTLSVTGTYY121 pKa = 10.4SGTPFAFSVVFPGANSTTATLSNTNYY147 pKa = 10.22GAATAPEE154 pKa = 4.11ISVSEE159 pKa = 4.1TGQNQGAVADD169 pKa = 4.67EE170 pKa = 4.38GTLAQGTQAAGSPVNLTFTVTNSGTGDD197 pKa = 3.54LTIATATSSLLSNVTVNSISAPTNTTVASGGGTEE231 pKa = 4.43TFTVQYY237 pKa = 9.74TPTSAGNFSFNLSFANDD254 pKa = 3.95DD255 pKa = 4.14ANEE258 pKa = 4.2GPYY261 pKa = 10.93NFIVSGTASGAPEE274 pKa = 3.68ISVAEE279 pKa = 4.27TGQSQGAVADD289 pKa = 4.42EE290 pKa = 4.38GTLAQGTQAAGSPVNLTFTVTNSGTGDD317 pKa = 3.54LTIATATSSLLSNVTVNSISAPTNSTVASGGGTEE351 pKa = 4.43TFTVQYY357 pKa = 9.74TPTSAGNFSFNLSFVNDD374 pKa = 4.35DD375 pKa = 4.29ANEE378 pKa = 4.0SPYY381 pKa = 11.27NFTISGTGTAAPTFSQSFSPTTIEE405 pKa = 3.62IGGASTITFTIDD417 pKa = 2.69NSTNALDD424 pKa = 3.79ATSLDD429 pKa = 3.87FTDD432 pKa = 4.42NLPAGMNVSSPSNAATTCTGGTLTASSGAGTVSYY466 pKa = 11.05SGGTVGAGASCTVSVDD482 pKa = 3.29VGATSDD488 pKa = 3.42GSFVNTTGNLTSSLGNSGPSSASLTVSSPDD518 pKa = 2.91IDD520 pKa = 3.72VQRR523 pKa = 11.84PVSTSIADD531 pKa = 4.26DD532 pKa = 5.4DD533 pKa = 4.33IDD535 pKa = 4.01SQGVVTLGVQQTLTFTIEE553 pKa = 4.2NKK555 pKa = 8.8GTATLSLSGTPTSDD569 pKa = 2.9SPINVSVDD577 pKa = 3.79SISAPGSNTLAGGATTTFTVQYY599 pKa = 9.02TPIAIGPFSFQVDD612 pKa = 3.56IVSNDD617 pKa = 3.17ADD619 pKa = 3.67EE620 pKa = 4.76ANYY623 pKa = 10.51DD624 pKa = 3.62ILVSGTASGAAEE636 pKa = 4.04IEE638 pKa = 4.52VSSSASGPISDD649 pKa = 5.1GGTDD653 pKa = 3.87TISGTVTAGSSATITYY669 pKa = 7.78TITNSGTAPLSLTQPTVGGNVTSTTNVTVDD699 pKa = 3.22SFTLSSLSVVSGGGTATLQVSYY721 pKa = 9.79TPTSSGSFDD730 pKa = 3.89FDD732 pKa = 4.99FNFANNDD739 pKa = 3.14ADD741 pKa = 3.94EE742 pKa = 4.6NPFNISVSGTGGGIPAGLSATSGSGQSTEE771 pKa = 4.48AGTQFGSVLVATVTDD786 pKa = 3.73SSNAGVAGVSVTFTAPSSGASLTFAGSGSNTEE818 pKa = 4.26TVITASDD825 pKa = 3.66GTATSSAMTANAIASGYY842 pKa = 10.38SGGSLQSYY850 pKa = 10.04AVTASAAGLTSVDD863 pKa = 4.33FFLTNSRR870 pKa = 11.84DD871 pKa = 3.45SAADD875 pKa = 3.19IQRR878 pKa = 11.84TEE880 pKa = 3.99DD881 pKa = 3.51VIASFVTSRR890 pKa = 11.84ANVIVAGQPDD900 pKa = 4.67LVSRR904 pKa = 11.84LTNGPFGRR912 pKa = 11.84QSGLNGFNFNASRR925 pKa = 11.84FSQTGSFQFSLRR937 pKa = 11.84AFSDD941 pKa = 3.42AVRR944 pKa = 11.84NGVSGSPVHH953 pKa = 6.2QPVTAAGHH961 pKa = 6.0AAGFDD966 pKa = 3.73SFTSEE971 pKa = 4.09TTSGYY976 pKa = 11.3ASAAPQAAEE985 pKa = 4.25AEE987 pKa = 4.41TLAILGIAAGQAPAAGEE1004 pKa = 3.96EE1005 pKa = 4.36SAEE1008 pKa = 4.27VQSGWDD1014 pKa = 3.67FWAEE1018 pKa = 3.5GTYY1021 pKa = 11.15ARR1023 pKa = 11.84TQDD1026 pKa = 3.46KK1027 pKa = 11.15ASDD1030 pKa = 3.93SVSGLFFAGLDD1041 pKa = 3.34YY1042 pKa = 11.09RR1043 pKa = 11.84FADD1046 pKa = 3.88RR1047 pKa = 11.84AVVGVLGQLDD1057 pKa = 3.5ITDD1060 pKa = 3.78EE1061 pKa = 4.62HH1062 pKa = 6.79NGSANTSADD1071 pKa = 3.68GIGWMVGPYY1080 pKa = 10.37AVLKK1084 pKa = 8.75VHH1086 pKa = 6.06QNFYY1090 pKa = 10.66IDD1092 pKa = 3.56GAVTYY1097 pKa = 8.58GQSNNSVNALGLFEE1111 pKa = 6.75DD1112 pKa = 4.66DD1113 pKa = 4.71FRR1115 pKa = 11.84TQRR1118 pKa = 11.84FLMQGGLTGDD1128 pKa = 3.99FEE1130 pKa = 5.54IDD1132 pKa = 2.72EE1133 pKa = 4.66HH1134 pKa = 8.56ARR1136 pKa = 11.84ISPFVRR1142 pKa = 11.84LTYY1145 pKa = 10.32YY1146 pKa = 10.74YY1147 pKa = 10.09EE1148 pKa = 4.16EE1149 pKa = 4.32QEE1151 pKa = 4.64SYY1153 pKa = 10.89TDD1155 pKa = 3.4SLGNQIPSQDD1165 pKa = 3.19FDD1167 pKa = 3.83LGRR1170 pKa = 11.84VEE1172 pKa = 5.2FGPKK1176 pKa = 9.76ISWEE1180 pKa = 4.36LILDD1184 pKa = 4.07DD1185 pKa = 5.25SLLFSPFVSLSGIYY1199 pKa = 10.23DD1200 pKa = 3.5FNKK1203 pKa = 10.04LHH1205 pKa = 6.08EE1206 pKa = 4.64TVPTDD1211 pKa = 3.34PRR1213 pKa = 11.84LASSDD1218 pKa = 3.19EE1219 pKa = 3.97DD1220 pKa = 3.31LRR1222 pKa = 11.84ARR1224 pKa = 11.84LEE1226 pKa = 3.9MGAGIVIPNRR1236 pKa = 11.84KK1237 pKa = 8.73IRR1239 pKa = 11.84ISGKK1243 pKa = 10.14GFYY1246 pKa = 10.29DD1247 pKa = 4.71GIGAADD1253 pKa = 4.68FSSYY1257 pKa = 11.26GGTLNFTVPFF1267 pKa = 4.53

Molecular weight: 128.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R5PM99|A0A4R5PM99_9RHIZ XRE family transcriptional regulator OS=Pseudohoeflea suaedae OX=877384 GN=E2A64_02995 PE=4 SV=1
MM1 pKa = 7.62TKK3 pKa = 9.11RR4 pKa = 11.84TYY6 pKa = 10.36QPSKK10 pKa = 9.73LVRR13 pKa = 11.84KK14 pKa = 8.95RR15 pKa = 11.84RR16 pKa = 11.84HH17 pKa = 4.63GFRR20 pKa = 11.84SRR22 pKa = 11.84MAKK25 pKa = 9.42VGGRR29 pKa = 11.84KK30 pKa = 9.34VIAARR35 pKa = 11.84RR36 pKa = 11.84NRR38 pKa = 11.84GRR40 pKa = 11.84KK41 pKa = 9.03RR42 pKa = 11.84LSAA45 pKa = 4.03

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3469

0

3469

1121147

41

2560

323.2

35.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.854 ± 0.06

0.81 ± 0.013

6.054 ± 0.038

6.281 ± 0.041

3.892 ± 0.027

8.751 ± 0.05

1.982 ± 0.02

5.67 ± 0.032

3.549 ± 0.032

9.516 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.691 ± 0.022

2.792 ± 0.022

4.811 ± 0.031

2.802 ± 0.022

6.71 ± 0.046

5.732 ± 0.03

5.346 ± 0.027

7.249 ± 0.033

1.233 ± 0.015

2.274 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski