Amino acid dipepetide frequency for Pelagibacter phage HTVC031P

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.247AlaAla: 3.247 ± 0.512
0.464AlaCys: 0.464 ± 0.234
4.87AlaAsp: 4.87 ± 0.622
5.025AlaGlu: 5.025 ± 0.677
2.628AlaPhe: 2.628 ± 0.397
5.566AlaGly: 5.566 ± 1.183
1.005AlaHis: 1.005 ± 0.248
3.401AlaIle: 3.401 ± 0.517
6.494AlaLys: 6.494 ± 0.88
5.566AlaLeu: 5.566 ± 0.769
2.087AlaMet: 2.087 ± 0.395
6.803AlaAsn: 6.803 ± 1.191
2.396AlaPro: 2.396 ± 0.657
3.633AlaGln: 3.633 ± 0.654
2.087AlaArg: 2.087 ± 0.347
6.648AlaSer: 6.648 ± 0.8
5.643AlaThr: 5.643 ± 1.048
5.179AlaVal: 5.179 ± 0.619
1.391AlaTrp: 1.391 ± 0.344
2.551AlaTyr: 2.551 ± 0.459
0.0AlaXaa: 0.0 ± 0.0
Cys
0.541CysAla: 0.541 ± 0.201
0.077CysCys: 0.077 ± 0.068
0.541CysAsp: 0.541 ± 0.163
0.541CysGlu: 0.541 ± 0.196
0.464CysPhe: 0.464 ± 0.204
0.464CysGly: 0.464 ± 0.15
0.232CysHis: 0.232 ± 0.139
0.464CysIle: 0.464 ± 0.172
0.696CysLys: 0.696 ± 0.245
1.16CysLeu: 1.16 ± 0.337
0.077CysMet: 0.077 ± 0.077
0.541CysAsn: 0.541 ± 0.207
0.232CysPro: 0.232 ± 0.144
0.387CysGln: 0.387 ± 0.16
0.541CysArg: 0.541 ± 0.184
0.387CysSer: 0.387 ± 0.163
0.464CysThr: 0.464 ± 0.17
0.232CysVal: 0.232 ± 0.138
0.077CysTrp: 0.077 ± 0.072
0.077CysTyr: 0.077 ± 0.068
0.0CysXaa: 0.0 ± 0.0
Asp
5.952AspAla: 5.952 ± 0.884
0.696AspCys: 0.696 ± 0.238
2.551AspAsp: 2.551 ± 0.477
3.711AspGlu: 3.711 ± 0.492
2.706AspPhe: 2.706 ± 0.397
4.252AspGly: 4.252 ± 0.661
0.464AspHis: 0.464 ± 0.174
4.02AspIle: 4.02 ± 0.408
5.489AspLys: 5.489 ± 0.659
6.339AspLeu: 6.339 ± 0.687
1.469AspMet: 1.469 ± 0.357
3.169AspAsn: 3.169 ± 0.484
1.391AspPro: 1.391 ± 0.403
1.314AspGln: 1.314 ± 0.315
2.087AspArg: 2.087 ± 0.361
3.015AspSer: 3.015 ± 0.398
4.02AspThr: 4.02 ± 0.782
4.252AspVal: 4.252 ± 0.893
1.005AspTrp: 1.005 ± 0.268
2.783AspTyr: 2.783 ± 0.407
0.0AspXaa: 0.0 ± 0.0
Glu
5.025GluAla: 5.025 ± 0.861
0.696GluCys: 0.696 ± 0.339
3.324GluAsp: 3.324 ± 0.488
4.174GluGlu: 4.174 ± 0.563
2.165GluPhe: 2.165 ± 0.378
4.097GluGly: 4.097 ± 0.661
1.16GluHis: 1.16 ± 0.287
4.329GluIle: 4.329 ± 0.506
5.952GluLys: 5.952 ± 0.837
5.643GluLeu: 5.643 ± 0.875
2.242GluMet: 2.242 ± 0.345
2.783GluAsn: 2.783 ± 0.423
1.778GluPro: 1.778 ± 0.485
2.165GluGln: 2.165 ± 0.356
2.86GluArg: 2.86 ± 0.561
2.087GluSer: 2.087 ± 0.353
5.257GluThr: 5.257 ± 0.619
3.865GluVal: 3.865 ± 0.684
0.85GluTrp: 0.85 ± 0.326
2.396GluTyr: 2.396 ± 0.503
0.0GluXaa: 0.0 ± 0.0
Phe
2.01PheAla: 2.01 ± 0.469
0.387PheCys: 0.387 ± 0.178
2.938PheAsp: 2.938 ± 0.647
2.938PheGlu: 2.938 ± 0.493
1.469PhePhe: 1.469 ± 0.372
2.087PheGly: 2.087 ± 0.446
0.618PheHis: 0.618 ± 0.215
1.855PheIle: 1.855 ± 0.331
3.942PheLys: 3.942 ± 0.532
2.628PheLeu: 2.628 ± 0.485
1.237PheMet: 1.237 ± 0.367
2.319PheAsn: 2.319 ± 0.389
1.237PhePro: 1.237 ± 0.387
1.933PheGln: 1.933 ± 0.379
1.469PheArg: 1.469 ± 0.297
2.628PheSer: 2.628 ± 0.413
2.938PheThr: 2.938 ± 0.448
1.623PheVal: 1.623 ± 0.288
0.232PheTrp: 0.232 ± 0.129
1.469PheTyr: 1.469 ± 0.289
0.0PheXaa: 0.0 ± 0.0
Gly
4.02GlyAla: 4.02 ± 0.575
0.309GlyCys: 0.309 ± 0.147
4.484GlyAsp: 4.484 ± 0.48
2.938GlyGlu: 2.938 ± 0.389
3.015GlyPhe: 3.015 ± 0.488
4.716GlyGly: 4.716 ± 0.599
1.237GlyHis: 1.237 ± 0.315
4.097GlyIle: 4.097 ± 0.639
4.793GlyLys: 4.793 ± 0.773
4.947GlyLeu: 4.947 ± 0.545
1.391GlyMet: 1.391 ± 0.312
3.711GlyAsn: 3.711 ± 0.69
0.0GlyPro: 0.0 ± 0.0
2.86GlyGln: 2.86 ± 0.509
2.551GlyArg: 2.551 ± 0.441
5.952GlySer: 5.952 ± 1.013
5.566GlyThr: 5.566 ± 1.126
4.252GlyVal: 4.252 ± 0.552
0.85GlyTrp: 0.85 ± 0.268
2.551GlyTyr: 2.551 ± 0.465
0.0GlyXaa: 0.0 ± 0.0
His
1.16HisAla: 1.16 ± 0.291
0.155HisCys: 0.155 ± 0.098
0.773HisAsp: 0.773 ± 0.321
0.696HisGlu: 0.696 ± 0.241
0.85HisPhe: 0.85 ± 0.206
1.16HisGly: 1.16 ± 0.295
0.387HisHis: 0.387 ± 0.187
1.314HisIle: 1.314 ± 0.301
0.928HisLys: 0.928 ± 0.27
1.469HisLeu: 1.469 ± 0.408
0.618HisMet: 0.618 ± 0.255
0.773HisAsn: 0.773 ± 0.207
0.773HisPro: 0.773 ± 0.21
0.541HisGln: 0.541 ± 0.207
0.85HisArg: 0.85 ± 0.257
1.005HisSer: 1.005 ± 0.226
1.314HisThr: 1.314 ± 0.338
0.696HisVal: 0.696 ± 0.204
0.464HisTrp: 0.464 ± 0.166
0.464HisTyr: 0.464 ± 0.185
0.0HisXaa: 0.0 ± 0.0
Ile
6.416IleAla: 6.416 ± 0.631
0.696IleCys: 0.696 ± 0.252
4.252IleAsp: 4.252 ± 0.771
5.102IleGlu: 5.102 ± 0.667
1.391IlePhe: 1.391 ± 0.332
3.633IleGly: 3.633 ± 0.616
1.005IleHis: 1.005 ± 0.405
3.865IleIle: 3.865 ± 0.685
5.72IleLys: 5.72 ± 0.675
3.865IleLeu: 3.865 ± 0.516
1.546IleMet: 1.546 ± 0.423
3.865IleAsn: 3.865 ± 0.632
2.474IlePro: 2.474 ± 0.462
2.319IleGln: 2.319 ± 0.479
2.319IleArg: 2.319 ± 0.473
3.865IleSer: 3.865 ± 0.759
3.788IleThr: 3.788 ± 0.515
3.556IleVal: 3.556 ± 0.46
0.541IleTrp: 0.541 ± 0.156
2.087IleTyr: 2.087 ± 0.43
0.0IleXaa: 0.0 ± 0.0
Lys
6.648LysAla: 6.648 ± 0.812
0.618LysCys: 0.618 ± 0.224
4.484LysAsp: 4.484 ± 1.037
6.88LysGlu: 6.88 ± 1.032
2.706LysPhe: 2.706 ± 0.555
3.711LysGly: 3.711 ± 0.497
1.701LysHis: 1.701 ± 0.362
5.643LysIle: 5.643 ± 0.666
6.88LysLys: 6.88 ± 1.137
8.271LysLeu: 8.271 ± 1.033
1.778LysMet: 1.778 ± 0.39
4.252LysAsn: 4.252 ± 0.539
4.02LysPro: 4.02 ± 0.562
3.788LysGln: 3.788 ± 0.626
4.252LysArg: 4.252 ± 0.86
3.788LysSer: 3.788 ± 0.579
5.025LysThr: 5.025 ± 0.623
5.025LysVal: 5.025 ± 0.579
1.082LysTrp: 1.082 ± 0.303
2.783LysTyr: 2.783 ± 0.498
0.0LysXaa: 0.0 ± 0.0
Leu
6.339LeuAla: 6.339 ± 0.781
0.85LeuCys: 0.85 ± 0.29
5.411LeuAsp: 5.411 ± 0.507
5.179LeuGlu: 5.179 ± 0.575
2.706LeuPhe: 2.706 ± 0.392
4.097LeuGly: 4.097 ± 0.45
1.546LeuHis: 1.546 ± 0.326
4.638LeuIle: 4.638 ± 0.502
6.416LeuLys: 6.416 ± 0.659
6.107LeuLeu: 6.107 ± 0.666
2.087LeuMet: 2.087 ± 0.431
5.025LeuAsn: 5.025 ± 0.516
3.401LeuPro: 3.401 ± 0.694
3.324LeuGln: 3.324 ± 0.519
2.86LeuArg: 2.86 ± 0.498
5.257LeuSer: 5.257 ± 0.715
6.03LeuThr: 6.03 ± 0.601
4.406LeuVal: 4.406 ± 0.689
1.237LeuTrp: 1.237 ± 0.302
2.783LeuTyr: 2.783 ± 0.423
0.0LeuXaa: 0.0 ± 0.0
Met
2.474MetAla: 2.474 ± 0.336
0.387MetCys: 0.387 ± 0.153
1.082MetAsp: 1.082 ± 0.212
0.773MetGlu: 0.773 ± 0.246
0.773MetPhe: 0.773 ± 0.208
1.391MetGly: 1.391 ± 0.29
0.309MetHis: 0.309 ± 0.142
1.314MetIle: 1.314 ± 0.307
2.242MetLys: 2.242 ± 0.345
2.165MetLeu: 2.165 ± 0.429
0.464MetMet: 0.464 ± 0.187
1.237MetAsn: 1.237 ± 0.4
1.082MetPro: 1.082 ± 0.278
0.928MetGln: 0.928 ± 0.262
1.314MetArg: 1.314 ± 0.343
2.087MetSer: 2.087 ± 0.433
1.778MetThr: 1.778 ± 0.386
1.082MetVal: 1.082 ± 0.284
0.155MetTrp: 0.155 ± 0.149
0.696MetTyr: 0.696 ± 0.212
0.0MetXaa: 0.0 ± 0.0
Asn
3.942AsnAla: 3.942 ± 0.796
0.232AsnCys: 0.232 ± 0.108
2.938AsnAsp: 2.938 ± 0.498
2.474AsnGlu: 2.474 ± 0.443
2.628AsnPhe: 2.628 ± 0.438
5.411AsnGly: 5.411 ± 1.053
0.928AsnHis: 0.928 ± 0.229
5.334AsnIle: 5.334 ± 0.837
5.643AsnLys: 5.643 ± 0.88
5.025AsnLeu: 5.025 ± 0.514
1.469AsnMet: 1.469 ± 0.324
5.025AsnAsn: 5.025 ± 0.704
2.242AsnPro: 2.242 ± 0.413
1.933AsnGln: 1.933 ± 0.404
2.087AsnArg: 2.087 ± 0.333
4.406AsnSer: 4.406 ± 0.747
5.025AsnThr: 5.025 ± 0.853
4.484AsnVal: 4.484 ± 0.962
0.773AsnTrp: 0.773 ± 0.245
2.706AsnTyr: 2.706 ± 0.48
0.0AsnXaa: 0.0 ± 0.0
Pro
1.933ProAla: 1.933 ± 0.314
0.387ProCys: 0.387 ± 0.17
2.242ProAsp: 2.242 ± 0.323
3.169ProGlu: 3.169 ± 0.649
1.778ProPhe: 1.778 ± 0.328
0.155ProGly: 0.155 ± 0.098
0.387ProHis: 0.387 ± 0.152
1.778ProIle: 1.778 ± 0.434
2.242ProLys: 2.242 ± 0.426
2.783ProLeu: 2.783 ± 0.47
0.387ProMet: 0.387 ± 0.183
1.933ProAsn: 1.933 ± 0.337
0.773ProPro: 0.773 ± 0.227
1.237ProGln: 1.237 ± 0.339
0.464ProArg: 0.464 ± 0.179
2.938ProSer: 2.938 ± 0.429
3.169ProThr: 3.169 ± 0.482
2.087ProVal: 2.087 ± 0.34
0.0ProTrp: 0.0 ± 0.0
1.314ProTyr: 1.314 ± 0.361
0.0ProXaa: 0.0 ± 0.0
Gln
3.865GlnAla: 3.865 ± 0.621
0.0GlnCys: 0.0 ± 0.0
2.01GlnAsp: 2.01 ± 0.48
3.092GlnGlu: 3.092 ± 0.708
1.701GlnPhe: 1.701 ± 0.285
1.933GlnGly: 1.933 ± 0.306
0.928GlnHis: 0.928 ± 0.31
2.706GlnIle: 2.706 ± 0.576
3.479GlnLys: 3.479 ± 0.602
3.247GlnLeu: 3.247 ± 0.576
0.618GlnMet: 0.618 ± 0.217
2.01GlnAsn: 2.01 ± 0.436
0.696GlnPro: 0.696 ± 0.25
2.474GlnGln: 2.474 ± 0.507
1.701GlnArg: 1.701 ± 0.294
2.628GlnSer: 2.628 ± 0.443
3.015GlnThr: 3.015 ± 0.454
1.778GlnVal: 1.778 ± 0.39
0.464GlnTrp: 0.464 ± 0.162
1.855GlnTyr: 1.855 ± 0.363
0.0GlnXaa: 0.0 ± 0.0
Arg
2.242ArgAla: 2.242 ± 0.333
0.0ArgCys: 0.0 ± 0.0
2.706ArgAsp: 2.706 ± 0.621
2.628ArgGlu: 2.628 ± 0.465
1.391ArgPhe: 1.391 ± 0.413
2.165ArgGly: 2.165 ± 0.549
0.773ArgHis: 0.773 ± 0.247
2.628ArgIle: 2.628 ± 0.413
2.551ArgLys: 2.551 ± 0.451
2.783ArgLeu: 2.783 ± 0.523
1.778ArgMet: 1.778 ± 0.328
2.087ArgAsn: 2.087 ± 0.461
0.85ArgPro: 0.85 ± 0.26
1.546ArgGln: 1.546 ± 0.403
1.469ArgArg: 1.469 ± 0.316
2.087ArgSer: 2.087 ± 0.384
2.087ArgThr: 2.087 ± 0.44
1.855ArgVal: 1.855 ± 0.483
0.309ArgTrp: 0.309 ± 0.155
2.01ArgTyr: 2.01 ± 0.358
0.0ArgXaa: 0.0 ± 0.0
Ser
4.947SerAla: 4.947 ± 0.812
0.618SerCys: 0.618 ± 0.238
3.401SerAsp: 3.401 ± 0.632
3.169SerGlu: 3.169 ± 0.461
2.319SerPhe: 2.319 ± 0.484
5.411SerGly: 5.411 ± 1.097
1.391SerHis: 1.391 ± 0.3
4.947SerIle: 4.947 ± 0.505
5.489SerLys: 5.489 ± 0.671
4.561SerLeu: 4.561 ± 0.484
0.773SerMet: 0.773 ± 0.253
5.102SerAsn: 5.102 ± 0.842
1.701SerPro: 1.701 ± 0.293
2.706SerGln: 2.706 ± 0.538
2.01SerArg: 2.01 ± 0.402
5.72SerSer: 5.72 ± 1.049
4.561SerThr: 4.561 ± 0.731
4.561SerVal: 4.561 ± 0.532
0.85SerTrp: 0.85 ± 0.272
2.474SerTyr: 2.474 ± 0.511
0.0SerXaa: 0.0 ± 0.0
Thr
6.648ThrAla: 6.648 ± 0.949
0.387ThrCys: 0.387 ± 0.166
5.643ThrAsp: 5.643 ± 0.72
4.252ThrGlu: 4.252 ± 0.412
3.015ThrPhe: 3.015 ± 0.574
6.648ThrGly: 6.648 ± 0.905
0.773ThrHis: 0.773 ± 0.244
4.406ThrIle: 4.406 ± 0.692
4.02ThrLys: 4.02 ± 0.598
4.406ThrLeu: 4.406 ± 0.661
1.469ThrMet: 1.469 ± 0.289
5.566ThrAsn: 5.566 ± 1.048
3.324ThrPro: 3.324 ± 0.468
3.092ThrGln: 3.092 ± 0.444
2.01ThrArg: 2.01 ± 0.288
4.561ThrSer: 4.561 ± 0.763
5.334ThrThr: 5.334 ± 1.223
6.03ThrVal: 6.03 ± 1.123
0.387ThrTrp: 0.387 ± 0.172
2.938ThrTyr: 2.938 ± 0.364
0.0ThrXaa: 0.0 ± 0.0
Val
6.03ValAla: 6.03 ± 1.411
0.696ValCys: 0.696 ± 0.274
4.252ValAsp: 4.252 ± 0.569
4.406ValGlu: 4.406 ± 0.497
1.778ValPhe: 1.778 ± 0.418
4.097ValGly: 4.097 ± 0.417
0.773ValHis: 0.773 ± 0.285
3.169ValIle: 3.169 ± 0.43
5.72ValLys: 5.72 ± 0.693
3.942ValLeu: 3.942 ± 0.401
1.082ValMet: 1.082 ± 0.326
5.257ValAsn: 5.257 ± 1.03
1.778ValPro: 1.778 ± 0.297
2.165ValGln: 2.165 ± 0.489
2.01ValArg: 2.01 ± 0.347
4.484ValSer: 4.484 ± 0.66
4.793ValThr: 4.793 ± 0.934
2.86ValVal: 2.86 ± 0.452
0.618ValTrp: 0.618 ± 0.185
1.469ValTyr: 1.469 ± 0.412
0.0ValXaa: 0.0 ± 0.0
Trp
0.541TrpAla: 0.541 ± 0.227
0.077TrpCys: 0.077 ± 0.075
0.541TrpAsp: 0.541 ± 0.199
0.387TrpGlu: 0.387 ± 0.175
0.618TrpPhe: 0.618 ± 0.255
0.387TrpGly: 0.387 ± 0.155
0.077TrpHis: 0.077 ± 0.078
0.464TrpIle: 0.464 ± 0.206
1.237TrpLys: 1.237 ± 0.218
1.623TrpLeu: 1.623 ± 0.335
0.232TrpMet: 0.232 ± 0.156
0.387TrpAsn: 0.387 ± 0.157
0.0TrpPro: 0.0 ± 0.0
0.309TrpGln: 0.309 ± 0.151
0.309TrpArg: 0.309 ± 0.146
1.237TrpSer: 1.237 ± 0.355
2.01TrpThr: 2.01 ± 0.461
1.16TrpVal: 1.16 ± 0.28
0.077TrpTrp: 0.077 ± 0.073
0.232TrpTyr: 0.232 ± 0.142
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.706TyrAla: 2.706 ± 0.369
0.464TyrCys: 0.464 ± 0.204
2.242TyrAsp: 2.242 ± 0.284
1.391TyrGlu: 1.391 ± 0.285
1.855TyrPhe: 1.855 ± 0.372
2.86TyrGly: 2.86 ± 0.521
0.696TyrHis: 0.696 ± 0.22
1.933TyrIle: 1.933 ± 0.439
3.401TyrLys: 3.401 ± 0.523
3.169TyrLeu: 3.169 ± 0.403
0.85TyrMet: 0.85 ± 0.225
2.86TyrAsn: 2.86 ± 0.537
1.005TyrPro: 1.005 ± 0.275
1.546TyrGln: 1.546 ± 0.387
0.696TyrArg: 0.696 ± 0.177
1.933TyrSer: 1.933 ± 0.404
3.092TyrThr: 3.092 ± 0.558
2.396TyrVal: 2.396 ± 0.474
0.541TyrTrp: 0.541 ± 0.218
1.391TyrTyr: 1.391 ± 0.298
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 53 proteins (12937 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski