Amino acid dipepetide frequency for Mycobacterium phage TM4 (Mycobacteriophage TM4)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.791AlaAla: 19.791 ± 1.542
1.073AlaCys: 1.073 ± 0.276
9.419AlaAsp: 9.419 ± 0.689
9.836AlaGlu: 9.836 ± 1.173
2.623AlaPhe: 2.623 ± 0.368
8.107AlaGly: 8.107 ± 0.9
3.398AlaHis: 3.398 ± 0.417
5.484AlaIle: 5.484 ± 0.633
4.65AlaLys: 4.65 ± 0.515
11.863AlaLeu: 11.863 ± 0.911
4.292AlaMet: 4.292 ± 0.538
2.742AlaAsn: 2.742 ± 0.476
6.677AlaPro: 6.677 ± 0.605
5.425AlaGln: 5.425 ± 0.636
8.405AlaArg: 8.405 ± 0.744
5.007AlaSer: 5.007 ± 0.532
6.975AlaThr: 6.975 ± 0.546
10.194AlaVal: 10.194 ± 0.778
2.921AlaTrp: 2.921 ± 0.359
2.086AlaTyr: 2.086 ± 0.372
0.0AlaXaa: 0.0 ± 0.0
Cys
1.49CysAla: 1.49 ± 0.325
0.179CysCys: 0.179 ± 0.097
0.477CysAsp: 0.477 ± 0.213
0.656CysGlu: 0.656 ± 0.201
0.179CysPhe: 0.179 ± 0.105
0.954CysGly: 0.954 ± 0.196
0.358CysHis: 0.358 ± 0.138
0.298CysIle: 0.298 ± 0.13
0.298CysLys: 0.298 ± 0.167
0.656CysLeu: 0.656 ± 0.165
0.298CysMet: 0.298 ± 0.155
0.238CysAsn: 0.238 ± 0.114
0.835CysPro: 0.835 ± 0.243
0.417CysGln: 0.417 ± 0.175
0.894CysArg: 0.894 ± 0.231
0.835CysSer: 0.835 ± 0.226
0.477CysThr: 0.477 ± 0.182
0.775CysVal: 0.775 ± 0.259
0.179CysTrp: 0.179 ± 0.101
0.238CysTyr: 0.238 ± 0.117
0.0CysXaa: 0.0 ± 0.0
Asp
8.107AspAla: 8.107 ± 0.543
0.775AspCys: 0.775 ± 0.233
5.604AspAsp: 5.604 ± 0.931
4.888AspGlu: 4.888 ± 0.572
1.61AspPhe: 1.61 ± 0.293
6.2AspGly: 6.2 ± 0.575
0.715AspHis: 0.715 ± 0.2
1.431AspIle: 1.431 ± 0.316
2.742AspLys: 2.742 ± 0.473
5.484AspLeu: 5.484 ± 0.57
1.967AspMet: 1.967 ± 0.367
2.206AspAsn: 2.206 ± 0.355
5.842AspPro: 5.842 ± 0.661
1.49AspGln: 1.49 ± 0.278
4.471AspArg: 4.471 ± 0.527
3.219AspSer: 3.219 ± 0.576
3.756AspThr: 3.756 ± 0.389
4.65AspVal: 4.65 ± 0.585
0.775AspTrp: 0.775 ± 0.215
1.55AspTyr: 1.55 ± 0.333
0.0AspXaa: 0.0 ± 0.0
Glu
7.332GluAla: 7.332 ± 0.803
1.013GluCys: 1.013 ± 0.285
2.504GluAsp: 2.504 ± 0.381
2.027GluGlu: 2.027 ± 0.379
1.431GluPhe: 1.431 ± 0.278
4.411GluGly: 4.411 ± 0.573
2.027GluHis: 2.027 ± 0.357
2.206GluIle: 2.206 ± 0.309
1.848GluLys: 1.848 ± 0.418
5.723GluLeu: 5.723 ± 0.577
1.133GluMet: 1.133 ± 0.307
1.073GluAsn: 1.073 ± 0.285
3.577GluPro: 3.577 ± 0.512
3.458GluGln: 3.458 ± 0.528
5.306GluArg: 5.306 ± 0.62
2.683GluSer: 2.683 ± 0.332
1.431GluThr: 1.431 ± 0.216
5.306GluVal: 5.306 ± 0.812
1.073GluTrp: 1.073 ± 0.262
1.49GluTyr: 1.49 ± 0.36
0.0GluXaa: 0.0 ± 0.0
Phe
2.683PheAla: 2.683 ± 0.496
0.238PheCys: 0.238 ± 0.107
2.981PheAsp: 2.981 ± 0.397
1.788PheGlu: 1.788 ± 0.266
0.596PhePhe: 0.596 ± 0.189
2.623PheGly: 2.623 ± 0.432
0.417PheHis: 0.417 ± 0.179
1.252PheIle: 1.252 ± 0.283
1.311PheLys: 1.311 ± 0.375
1.61PheLeu: 1.61 ± 0.323
0.775PheMet: 0.775 ± 0.218
0.656PheAsn: 0.656 ± 0.208
0.715PhePro: 0.715 ± 0.223
0.894PheGln: 0.894 ± 0.195
2.206PheArg: 2.206 ± 0.336
0.835PheSer: 0.835 ± 0.196
1.848PheThr: 1.848 ± 0.305
2.206PheVal: 2.206 ± 0.407
0.477PheTrp: 0.477 ± 0.159
0.537PheTyr: 0.537 ± 0.174
0.0PheXaa: 0.0 ± 0.0
Gly
10.313GlyAla: 10.313 ± 0.796
0.656GlyCys: 0.656 ± 0.21
5.723GlyAsp: 5.723 ± 0.528
4.65GlyGlu: 4.65 ± 0.535
2.325GlyPhe: 2.325 ± 0.395
9.896GlyGly: 9.896 ± 1.258
2.146GlyHis: 2.146 ± 0.512
2.981GlyIle: 2.981 ± 0.449
5.067GlyLys: 5.067 ± 0.737
6.617GlyLeu: 6.617 ± 0.884
2.146GlyMet: 2.146 ± 0.407
3.517GlyAsn: 3.517 ± 0.521
3.636GlyPro: 3.636 ± 0.58
2.623GlyGln: 2.623 ± 0.38
5.604GlyArg: 5.604 ± 0.706
4.471GlySer: 4.471 ± 0.99
4.352GlyThr: 4.352 ± 0.584
6.617GlyVal: 6.617 ± 0.641
1.908GlyTrp: 1.908 ± 0.295
1.729GlyTyr: 1.729 ± 0.349
0.0GlyXaa: 0.0 ± 0.0
His
2.802HisAla: 2.802 ± 0.453
0.298HisCys: 0.298 ± 0.142
1.49HisAsp: 1.49 ± 0.366
0.775HisGlu: 0.775 ± 0.217
0.537HisPhe: 0.537 ± 0.184
1.788HisGly: 1.788 ± 0.371
0.596HisHis: 0.596 ± 0.182
1.192HisIle: 1.192 ± 0.268
0.656HisLys: 0.656 ± 0.202
2.086HisLeu: 2.086 ± 0.355
0.537HisMet: 0.537 ± 0.219
1.013HisAsn: 1.013 ± 0.212
1.61HisPro: 1.61 ± 0.326
1.013HisGln: 1.013 ± 0.283
2.385HisArg: 2.385 ± 0.38
0.775HisSer: 0.775 ± 0.228
1.073HisThr: 1.073 ± 0.267
1.848HisVal: 1.848 ± 0.282
0.537HisTrp: 0.537 ± 0.216
0.537HisTyr: 0.537 ± 0.188
0.0HisXaa: 0.0 ± 0.0
Ile
5.306IleAla: 5.306 ± 0.489
0.298IleCys: 0.298 ± 0.131
3.696IleAsp: 3.696 ± 0.469
3.219IleGlu: 3.219 ± 0.413
1.073IlePhe: 1.073 ± 0.287
3.1IleGly: 3.1 ± 0.513
0.596IleHis: 0.596 ± 0.162
1.073IleIle: 1.073 ± 0.365
1.848IleLys: 1.848 ± 0.549
3.219IleLeu: 3.219 ± 0.511
1.013IleMet: 1.013 ± 0.25
1.431IleAsn: 1.431 ± 0.28
2.385IlePro: 2.385 ± 0.433
0.715IleGln: 0.715 ± 0.233
2.206IleArg: 2.206 ± 0.396
2.086IleSer: 2.086 ± 0.397
2.504IleThr: 2.504 ± 0.385
2.742IleVal: 2.742 ± 0.455
0.417IleTrp: 0.417 ± 0.199
0.477IleTyr: 0.477 ± 0.186
0.0IleXaa: 0.0 ± 0.0
Lys
6.259LysAla: 6.259 ± 0.751
0.477LysCys: 0.477 ± 0.199
1.073LysAsp: 1.073 ± 0.262
0.596LysGlu: 0.596 ± 0.193
1.252LysPhe: 1.252 ± 0.336
3.338LysGly: 3.338 ± 0.767
0.954LysHis: 0.954 ± 0.25
1.371LysIle: 1.371 ± 0.266
0.298LysLys: 0.298 ± 0.139
3.875LysLeu: 3.875 ± 0.496
0.656LysMet: 0.656 ± 0.181
0.596LysAsn: 0.596 ± 0.231
2.444LysPro: 2.444 ± 0.307
1.073LysGln: 1.073 ± 0.231
3.338LysArg: 3.338 ± 0.471
2.027LysSer: 2.027 ± 0.367
1.967LysThr: 1.967 ± 0.378
3.219LysVal: 3.219 ± 0.443
0.894LysTrp: 0.894 ± 0.215
0.656LysTyr: 0.656 ± 0.222
0.0LysXaa: 0.0 ± 0.0
Leu
12.34LeuAla: 12.34 ± 0.803
0.596LeuCys: 0.596 ± 0.183
6.975LeuAsp: 6.975 ± 0.55
2.563LeuGlu: 2.563 ± 0.342
2.206LeuPhe: 2.206 ± 0.459
8.048LeuGly: 8.048 ± 0.746
1.848LeuHis: 1.848 ± 0.323
3.994LeuIle: 3.994 ± 0.48
2.683LeuLys: 2.683 ± 0.412
5.544LeuLeu: 5.544 ± 0.664
1.371LeuMet: 1.371 ± 0.268
2.027LeuAsn: 2.027 ± 0.36
5.127LeuPro: 5.127 ± 0.693
2.683LeuGln: 2.683 ± 0.519
5.604LeuArg: 5.604 ± 0.633
5.007LeuSer: 5.007 ± 0.564
5.604LeuThr: 5.604 ± 0.563
5.365LeuVal: 5.365 ± 0.561
1.61LeuTrp: 1.61 ± 0.256
1.55LeuTyr: 1.55 ± 0.347
0.0LeuXaa: 0.0 ± 0.0
Met
2.802MetAla: 2.802 ± 0.396
0.238MetCys: 0.238 ± 0.107
0.954MetAsp: 0.954 ± 0.193
0.417MetGlu: 0.417 ± 0.131
0.477MetPhe: 0.477 ± 0.187
1.371MetGly: 1.371 ± 0.306
0.656MetHis: 0.656 ± 0.215
1.013MetIle: 1.013 ± 0.252
0.537MetLys: 0.537 ± 0.253
1.669MetLeu: 1.669 ± 0.362
0.417MetMet: 0.417 ± 0.166
0.775MetAsn: 0.775 ± 0.185
0.954MetPro: 0.954 ± 0.219
1.192MetGln: 1.192 ± 0.281
2.086MetArg: 2.086 ± 0.381
2.444MetSer: 2.444 ± 0.354
2.325MetThr: 2.325 ± 0.379
2.146MetVal: 2.146 ± 0.394
0.537MetTrp: 0.537 ± 0.211
0.358MetTyr: 0.358 ± 0.181
0.0MetXaa: 0.0 ± 0.0
Asn
3.398AsnAla: 3.398 ± 0.341
0.298AsnCys: 0.298 ± 0.139
1.371AsnAsp: 1.371 ± 0.269
1.133AsnGlu: 1.133 ± 0.235
0.537AsnPhe: 0.537 ± 0.196
3.1AsnGly: 3.1 ± 0.423
0.775AsnHis: 0.775 ± 0.179
1.073AsnIle: 1.073 ± 0.326
0.417AsnLys: 0.417 ± 0.172
2.265AsnLeu: 2.265 ± 0.386
0.596AsnMet: 0.596 ± 0.161
0.358AsnAsn: 0.358 ± 0.152
2.385AsnPro: 2.385 ± 0.435
0.596AsnGln: 0.596 ± 0.159
1.788AsnArg: 1.788 ± 0.3
1.61AsnSer: 1.61 ± 0.318
1.669AsnThr: 1.669 ± 0.269
2.444AsnVal: 2.444 ± 0.335
0.656AsnTrp: 0.656 ± 0.179
0.596AsnTyr: 0.596 ± 0.187
0.0AsnXaa: 0.0 ± 0.0
Pro
7.809ProAla: 7.809 ± 0.854
0.715ProCys: 0.715 ± 0.29
3.934ProAsp: 3.934 ± 0.451
5.007ProGlu: 5.007 ± 0.563
1.371ProPhe: 1.371 ± 0.26
6.617ProGly: 6.617 ± 0.829
1.729ProHis: 1.729 ± 0.348
2.146ProIle: 2.146 ± 0.393
2.206ProLys: 2.206 ± 0.335
4.232ProLeu: 4.232 ± 0.578
0.954ProMet: 0.954 ± 0.236
1.371ProAsn: 1.371 ± 0.305
3.398ProPro: 3.398 ± 0.562
1.49ProGln: 1.49 ± 0.326
3.159ProArg: 3.159 ± 0.524
3.1ProSer: 3.1 ± 0.493
3.04ProThr: 3.04 ± 0.388
4.292ProVal: 4.292 ± 0.519
1.192ProTrp: 1.192 ± 0.277
1.192ProTyr: 1.192 ± 0.322
0.0ProXaa: 0.0 ± 0.0
Gln
5.484GlnAla: 5.484 ± 0.605
0.417GlnCys: 0.417 ± 0.134
1.788GlnAsp: 1.788 ± 0.381
0.894GlnGlu: 0.894 ± 0.239
1.192GlnPhe: 1.192 ± 0.229
2.981GlnGly: 2.981 ± 0.351
0.835GlnHis: 0.835 ± 0.202
1.55GlnIle: 1.55 ± 0.276
1.013GlnLys: 1.013 ± 0.261
2.921GlnLeu: 2.921 ± 0.412
0.656GlnMet: 0.656 ± 0.205
0.715GlnAsn: 0.715 ± 0.221
2.265GlnPro: 2.265 ± 0.37
1.967GlnGln: 1.967 ± 0.556
2.683GlnArg: 2.683 ± 0.506
1.192GlnSer: 1.192 ± 0.265
1.61GlnThr: 1.61 ± 0.308
2.385GlnVal: 2.385 ± 0.457
1.252GlnTrp: 1.252 ± 0.247
1.192GlnTyr: 1.192 ± 0.228
0.0GlnXaa: 0.0 ± 0.0
Arg
7.511ArgAla: 7.511 ± 0.762
1.133ArgCys: 1.133 ± 0.281
4.411ArgAsp: 4.411 ± 0.631
4.65ArgGlu: 4.65 ± 0.542
2.206ArgPhe: 2.206 ± 0.289
4.65ArgGly: 4.65 ± 0.488
1.61ArgHis: 1.61 ± 0.305
2.981ArgIle: 2.981 ± 0.458
3.517ArgLys: 3.517 ± 0.502
7.094ArgLeu: 7.094 ± 0.743
1.729ArgMet: 1.729 ± 0.369
2.146ArgAsn: 2.146 ± 0.337
3.577ArgPro: 3.577 ± 0.613
2.325ArgGln: 2.325 ± 0.318
6.438ArgArg: 6.438 ± 0.783
3.219ArgSer: 3.219 ± 0.314
3.815ArgThr: 3.815 ± 0.554
4.709ArgVal: 4.709 ± 0.575
1.55ArgTrp: 1.55 ± 0.344
1.848ArgTyr: 1.848 ± 0.342
0.0ArgXaa: 0.0 ± 0.0
Ser
5.902SerAla: 5.902 ± 0.69
0.298SerCys: 0.298 ± 0.146
2.981SerAsp: 2.981 ± 0.407
3.159SerGlu: 3.159 ± 0.409
1.788SerPhe: 1.788 ± 0.307
5.663SerGly: 5.663 ± 1.029
0.775SerHis: 0.775 ± 0.19
1.908SerIle: 1.908 ± 0.428
1.848SerLys: 1.848 ± 0.446
3.696SerLeu: 3.696 ± 0.603
1.61SerMet: 1.61 ± 0.274
1.133SerAsn: 1.133 ± 0.232
2.981SerPro: 2.981 ± 0.321
1.729SerGln: 1.729 ± 0.275
2.802SerArg: 2.802 ± 0.413
2.861SerSer: 2.861 ± 0.577
2.385SerThr: 2.385 ± 0.373
3.934SerVal: 3.934 ± 0.496
0.954SerTrp: 0.954 ± 0.285
1.133SerTyr: 1.133 ± 0.239
0.0SerXaa: 0.0 ± 0.0
Thr
7.63ThrAla: 7.63 ± 0.718
0.656ThrCys: 0.656 ± 0.185
3.815ThrAsp: 3.815 ± 0.394
3.219ThrGlu: 3.219 ± 0.539
2.265ThrPhe: 2.265 ± 0.345
4.709ThrGly: 4.709 ± 0.566
1.133ThrHis: 1.133 ± 0.323
2.981ThrIle: 2.981 ± 0.508
1.431ThrLys: 1.431 ± 0.359
3.338ThrLeu: 3.338 ± 0.565
1.013ThrMet: 1.013 ± 0.256
1.371ThrAsn: 1.371 ± 0.242
3.815ThrPro: 3.815 ± 0.545
1.729ThrGln: 1.729 ± 0.299
3.696ThrArg: 3.696 ± 0.489
2.623ThrSer: 2.623 ± 0.441
2.921ThrThr: 2.921 ± 0.409
4.65ThrVal: 4.65 ± 0.511
0.894ThrTrp: 0.894 ± 0.207
1.49ThrTyr: 1.49 ± 0.322
0.0ThrXaa: 0.0 ± 0.0
Val
9.598ValAla: 9.598 ± 0.906
0.894ValCys: 0.894 ± 0.196
5.544ValAsp: 5.544 ± 0.565
5.365ValGlu: 5.365 ± 0.624
1.848ValPhe: 1.848 ± 0.415
6.438ValGly: 6.438 ± 0.692
2.086ValHis: 2.086 ± 0.387
2.921ValIle: 2.921 ± 0.371
3.1ValLys: 3.1 ± 0.41
6.498ValLeu: 6.498 ± 0.759
1.431ValMet: 1.431 ± 0.252
2.385ValAsn: 2.385 ± 0.393
4.173ValPro: 4.173 ± 0.517
2.444ValGln: 2.444 ± 0.398
4.173ValArg: 4.173 ± 0.526
3.1ValSer: 3.1 ± 0.339
5.007ValThr: 5.007 ± 0.614
5.186ValVal: 5.186 ± 0.561
1.848ValTrp: 1.848 ± 0.287
1.55ValTyr: 1.55 ± 0.261
0.0ValXaa: 0.0 ± 0.0
Trp
1.788TrpAla: 1.788 ± 0.29
0.358TrpCys: 0.358 ± 0.144
0.715TrpAsp: 0.715 ± 0.187
1.073TrpGlu: 1.073 ± 0.278
0.715TrpPhe: 0.715 ± 0.202
1.192TrpGly: 1.192 ± 0.266
0.835TrpHis: 0.835 ± 0.201
1.133TrpIle: 1.133 ± 0.234
0.417TrpLys: 0.417 ± 0.155
2.504TrpLeu: 2.504 ± 0.337
0.298TrpMet: 0.298 ± 0.153
0.596TrpAsn: 0.596 ± 0.167
1.073TrpPro: 1.073 ± 0.249
0.954TrpGln: 0.954 ± 0.198
2.027TrpArg: 2.027 ± 0.345
1.431TrpSer: 1.431 ± 0.351
1.311TrpThr: 1.311 ± 0.347
1.192TrpVal: 1.192 ± 0.267
0.775TrpTrp: 0.775 ± 0.198
0.477TrpTyr: 0.477 ± 0.171
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.623TyrAla: 2.623 ± 0.402
0.119TyrCys: 0.119 ± 0.091
2.027TyrAsp: 2.027 ± 0.403
1.133TyrGlu: 1.133 ± 0.281
0.417TyrPhe: 0.417 ± 0.143
1.848TyrGly: 1.848 ± 0.338
0.06TyrHis: 0.06 ± 0.061
0.417TyrIle: 0.417 ± 0.138
0.477TyrLys: 0.477 ± 0.21
1.848TyrLeu: 1.848 ± 0.313
0.417TyrMet: 0.417 ± 0.165
0.715TyrAsn: 0.715 ± 0.175
1.49TyrPro: 1.49 ± 0.293
0.775TyrGln: 0.775 ± 0.252
1.848TyrArg: 1.848 ± 0.38
1.013TyrSer: 1.013 ± 0.236
1.311TyrThr: 1.311 ± 0.265
1.729TyrVal: 1.729 ± 0.346
0.417TyrTrp: 0.417 ± 0.145
0.537TyrTyr: 0.537 ± 0.173
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 92 proteins (16776 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski