Escherichia phage smaasur

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Bonnellvirus; unclassified Bonnellvirus

Average proteome isoelectric point is 5.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9X8M1|A0A6B9X8M1_9CAUD Putative polynucleotide 5' kinase/3' phosphatase OS=Escherichia phage smaasur OX=2696444 GN=smaasur_12 PE=4 SV=1
MM1 pKa = 7.59SKK3 pKa = 11.05LEE5 pKa = 3.95LGQRR9 pKa = 11.84VKK11 pKa = 11.11SRR13 pKa = 11.84WFGNAVGVVVYY24 pKa = 10.64RR25 pKa = 11.84GDD27 pKa = 3.5TGKK30 pKa = 10.54SLPVFDD36 pKa = 5.04AHH38 pKa = 7.24PDD40 pKa = 3.22ADD42 pKa = 4.05VYY44 pKa = 11.06RR45 pKa = 11.84GLASDD50 pKa = 4.68DD51 pKa = 4.09DD52 pKa = 4.45DD53 pKa = 5.79FIVLFDD59 pKa = 3.52EE60 pKa = 4.97AQAEE64 pKa = 4.53GVNGEE69 pKa = 4.06LAGVLGYY76 pKa = 10.88GYY78 pKa = 10.6DD79 pKa = 3.7DD80 pKa = 5.45DD81 pKa = 6.9GDD83 pKa = 4.46FEE85 pKa = 4.82VEE87 pKa = 3.88

Molecular weight: 9.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9X868|A0A6B9X868_9CAUD Exonuclease OS=Escherichia phage smaasur OX=2696444 GN=smaasur_16 PE=4 SV=1
MM1 pKa = 7.38GSVGKK6 pKa = 9.72VFKK9 pKa = 11.07GIGSVFGLGSQAPKK23 pKa = 9.57YY24 pKa = 8.68TIKK27 pKa = 10.03TDD29 pKa = 3.62PAQVKK34 pKa = 8.82AQAMQANLAADD45 pKa = 4.61LSNDD49 pKa = 3.37NTPTIQSGGQVSSASDD65 pKa = 3.31TLSGRR70 pKa = 11.84RR71 pKa = 11.84RR72 pKa = 11.84RR73 pKa = 11.84TGTGVASNLGIGSS86 pKa = 3.69

Molecular weight: 8.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

48

0

48

12600

50

1033

262.5

29.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.151 ± 0.555

0.992 ± 0.169

6.0 ± 0.253

6.238 ± 0.444

3.429 ± 0.236

7.81 ± 0.32

1.944 ± 0.266

4.659 ± 0.201

4.611 ± 0.344

8.048 ± 0.314

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.857 ± 0.224

4.302 ± 0.378

4.325 ± 0.354

4.937 ± 0.577

5.659 ± 0.29

5.587 ± 0.393

6.127 ± 0.423

7.159 ± 0.313

1.421 ± 0.149

3.746 ± 0.249
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski