Amino acid dipepetide frequency for Escherichia phage smaasur

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.779AlaAla: 12.779 ± 1.492
0.952AlaCys: 0.952 ± 0.287
5.477AlaAsp: 5.477 ± 0.587
6.429AlaGlu: 6.429 ± 0.699
3.334AlaPhe: 3.334 ± 0.573
7.143AlaGly: 7.143 ± 0.854
1.826AlaHis: 1.826 ± 0.4
4.683AlaIle: 4.683 ± 0.495
4.842AlaLys: 4.842 ± 0.627
9.128AlaLeu: 9.128 ± 1.012
3.016AlaMet: 3.016 ± 0.63
4.921AlaAsn: 4.921 ± 0.611
3.889AlaPro: 3.889 ± 0.697
4.286AlaGln: 4.286 ± 0.876
5.556AlaArg: 5.556 ± 0.659
5.953AlaSer: 5.953 ± 0.855
6.27AlaThr: 6.27 ± 0.911
6.35AlaVal: 6.35 ± 0.818
1.191AlaTrp: 1.191 ± 0.273
3.492AlaTyr: 3.492 ± 0.487
0.0AlaXaa: 0.0 ± 0.0
Cys
1.032CysAla: 1.032 ± 0.395
0.0CysCys: 0.0 ± 0.0
0.397CysAsp: 0.397 ± 0.247
0.714CysGlu: 0.714 ± 0.261
0.238CysPhe: 0.238 ± 0.129
0.952CysGly: 0.952 ± 0.38
0.238CysHis: 0.238 ± 0.143
0.476CysIle: 0.476 ± 0.209
0.556CysLys: 0.556 ± 0.267
0.397CysLeu: 0.397 ± 0.177
0.397CysMet: 0.397 ± 0.191
0.317CysAsn: 0.317 ± 0.231
0.476CysPro: 0.476 ± 0.23
0.317CysGln: 0.317 ± 0.179
0.556CysArg: 0.556 ± 0.273
0.714CysSer: 0.714 ± 0.23
0.556CysThr: 0.556 ± 0.218
0.794CysVal: 0.794 ± 0.261
0.238CysTrp: 0.238 ± 0.153
0.556CysTyr: 0.556 ± 0.23
0.0CysXaa: 0.0 ± 0.0
Asp
6.032AspAla: 6.032 ± 0.459
0.397AspCys: 0.397 ± 0.202
4.683AspAsp: 4.683 ± 0.743
4.286AspGlu: 4.286 ± 0.641
2.381AspPhe: 2.381 ± 0.57
5.397AspGly: 5.397 ± 0.807
0.873AspHis: 0.873 ± 0.283
3.016AspIle: 3.016 ± 0.392
2.778AspLys: 2.778 ± 0.483
3.969AspLeu: 3.969 ± 0.43
2.143AspMet: 2.143 ± 0.574
2.381AspAsn: 2.381 ± 0.418
3.413AspPro: 3.413 ± 0.558
1.111AspGln: 1.111 ± 0.39
2.857AspArg: 2.857 ± 0.345
3.81AspSer: 3.81 ± 0.667
3.254AspThr: 3.254 ± 0.387
3.572AspVal: 3.572 ± 0.551
1.429AspTrp: 1.429 ± 0.403
2.222AspTyr: 2.222 ± 0.455
0.0AspXaa: 0.0 ± 0.0
Glu
5.556GluAla: 5.556 ± 0.93
0.635GluCys: 0.635 ± 0.269
3.254GluAsp: 3.254 ± 0.584
4.127GluGlu: 4.127 ± 0.883
2.857GluPhe: 2.857 ± 0.526
4.207GluGly: 4.207 ± 0.518
1.349GluHis: 1.349 ± 0.275
2.143GluIle: 2.143 ± 0.447
3.016GluLys: 3.016 ± 0.542
5.477GluLeu: 5.477 ± 0.825
1.984GluMet: 1.984 ± 0.342
2.064GluAsn: 2.064 ± 0.402
2.54GluPro: 2.54 ± 0.656
3.492GluGln: 3.492 ± 0.479
3.175GluArg: 3.175 ± 0.517
3.572GluSer: 3.572 ± 0.517
3.651GluThr: 3.651 ± 0.5
5.397GluVal: 5.397 ± 0.679
0.794GluTrp: 0.794 ± 0.251
3.095GluTyr: 3.095 ± 0.547
0.0GluXaa: 0.0 ± 0.0
Phe
2.064PheAla: 2.064 ± 0.439
0.476PheCys: 0.476 ± 0.17
3.095PheAsp: 3.095 ± 0.602
1.746PheGlu: 1.746 ± 0.386
0.794PhePhe: 0.794 ± 0.217
3.334PheGly: 3.334 ± 0.389
0.556PheHis: 0.556 ± 0.177
2.461PheIle: 2.461 ± 0.436
2.143PheLys: 2.143 ± 0.485
2.222PheLeu: 2.222 ± 0.409
1.191PheMet: 1.191 ± 0.253
2.143PheAsn: 2.143 ± 0.456
1.667PhePro: 1.667 ± 0.343
1.191PheGln: 1.191 ± 0.29
1.429PheArg: 1.429 ± 0.338
1.667PheSer: 1.667 ± 0.387
2.461PheThr: 2.461 ± 0.528
2.302PheVal: 2.302 ± 0.263
0.476PheTrp: 0.476 ± 0.191
0.873PheTyr: 0.873 ± 0.259
0.0PheXaa: 0.0 ± 0.0
Gly
7.064GlyAla: 7.064 ± 0.92
1.032GlyCys: 1.032 ± 0.348
4.445GlyAsp: 4.445 ± 0.559
4.207GlyGlu: 4.207 ± 0.625
2.064GlyPhe: 2.064 ± 0.442
6.27GlyGly: 6.27 ± 0.868
1.508GlyHis: 1.508 ± 0.417
4.842GlyIle: 4.842 ± 0.476
4.127GlyLys: 4.127 ± 0.862
5.794GlyLeu: 5.794 ± 0.621
2.064GlyMet: 2.064 ± 0.444
3.175GlyAsn: 3.175 ± 0.676
1.508GlyPro: 1.508 ± 0.307
4.207GlyGln: 4.207 ± 0.691
5.318GlyArg: 5.318 ± 0.493
4.683GlySer: 4.683 ± 0.851
5.0GlyThr: 5.0 ± 1.037
6.27GlyVal: 6.27 ± 0.938
1.27GlyTrp: 1.27 ± 0.311
3.254GlyTyr: 3.254 ± 0.423
0.0GlyXaa: 0.0 ± 0.0
His
2.143HisAla: 2.143 ± 0.431
0.317HisCys: 0.317 ± 0.153
1.429HisAsp: 1.429 ± 0.338
1.349HisGlu: 1.349 ± 0.316
0.794HisPhe: 0.794 ± 0.252
1.111HisGly: 1.111 ± 0.321
0.714HisHis: 0.714 ± 0.309
0.714HisIle: 0.714 ± 0.242
1.111HisLys: 1.111 ± 0.322
2.222HisLeu: 2.222 ± 0.526
0.556HisMet: 0.556 ± 0.177
0.476HisAsn: 0.476 ± 0.193
0.635HisPro: 0.635 ± 0.192
0.635HisGln: 0.635 ± 0.236
0.714HisArg: 0.714 ± 0.28
1.032HisSer: 1.032 ± 0.27
0.952HisThr: 0.952 ± 0.312
1.429HisVal: 1.429 ± 0.408
0.397HisTrp: 0.397 ± 0.23
0.714HisTyr: 0.714 ± 0.184
0.0HisXaa: 0.0 ± 0.0
Ile
4.127IleAla: 4.127 ± 0.409
0.635IleCys: 0.635 ± 0.365
3.492IleAsp: 3.492 ± 0.521
3.175IleGlu: 3.175 ± 0.368
1.191IlePhe: 1.191 ± 0.268
3.095IleGly: 3.095 ± 0.621
1.191IleHis: 1.191 ± 0.29
2.064IleIle: 2.064 ± 0.526
2.222IleLys: 2.222 ± 0.415
2.619IleLeu: 2.619 ± 0.415
1.508IleMet: 1.508 ± 0.325
1.667IleAsn: 1.667 ± 0.412
2.778IlePro: 2.778 ± 0.42
2.699IleGln: 2.699 ± 0.466
3.254IleArg: 3.254 ± 0.488
2.143IleSer: 2.143 ± 0.429
2.54IleThr: 2.54 ± 0.475
3.889IleVal: 3.889 ± 0.684
0.476IleTrp: 0.476 ± 0.188
1.826IleTyr: 1.826 ± 0.426
0.0IleXaa: 0.0 ± 0.0
Lys
5.477LysAla: 5.477 ± 0.914
0.635LysCys: 0.635 ± 0.224
2.619LysAsp: 2.619 ± 0.561
2.699LysGlu: 2.699 ± 0.471
1.746LysPhe: 1.746 ± 0.414
3.095LysGly: 3.095 ± 0.494
0.714LysHis: 0.714 ± 0.232
0.397LysIle: 0.397 ± 0.178
1.508LysLys: 1.508 ± 0.49
4.921LysLeu: 4.921 ± 0.8
0.952LysMet: 0.952 ± 0.267
1.349LysAsn: 1.349 ± 0.273
1.587LysPro: 1.587 ± 0.309
2.461LysGln: 2.461 ± 0.508
3.334LysArg: 3.334 ± 0.527
3.651LysSer: 3.651 ± 0.509
3.016LysThr: 3.016 ± 0.466
3.81LysVal: 3.81 ± 0.585
0.873LysTrp: 0.873 ± 0.296
1.27LysTyr: 1.27 ± 0.354
0.0LysXaa: 0.0 ± 0.0
Leu
8.89LeuAla: 8.89 ± 0.669
0.556LeuCys: 0.556 ± 0.188
5.08LeuAsp: 5.08 ± 0.622
5.318LeuGlu: 5.318 ± 0.465
3.334LeuPhe: 3.334 ± 0.464
5.556LeuGly: 5.556 ± 0.684
1.905LeuHis: 1.905 ± 0.496
3.572LeuIle: 3.572 ± 0.743
2.857LeuLys: 2.857 ± 0.43
6.905LeuLeu: 6.905 ± 0.95
1.905LeuMet: 1.905 ± 0.455
3.254LeuAsn: 3.254 ± 0.591
4.286LeuPro: 4.286 ± 0.706
3.889LeuGln: 3.889 ± 0.617
4.604LeuArg: 4.604 ± 0.697
5.477LeuSer: 5.477 ± 0.572
5.556LeuThr: 5.556 ± 0.668
4.365LeuVal: 4.365 ± 0.5
1.111LeuTrp: 1.111 ± 0.294
2.064LeuTyr: 2.064 ± 0.393
0.0LeuXaa: 0.0 ± 0.0
Met
2.778MetAla: 2.778 ± 0.449
0.238MetCys: 0.238 ± 0.135
1.429MetAsp: 1.429 ± 0.337
0.952MetGlu: 0.952 ± 0.282
0.714MetPhe: 0.714 ± 0.263
2.064MetGly: 2.064 ± 0.431
0.635MetHis: 0.635 ± 0.213
0.476MetIle: 0.476 ± 0.192
1.349MetLys: 1.349 ± 0.291
2.778MetLeu: 2.778 ± 0.51
1.27MetMet: 1.27 ± 0.433
1.508MetAsn: 1.508 ± 0.315
1.111MetPro: 1.111 ± 0.277
1.587MetGln: 1.587 ± 0.431
1.667MetArg: 1.667 ± 0.41
1.826MetSer: 1.826 ± 0.408
2.302MetThr: 2.302 ± 0.338
2.064MetVal: 2.064 ± 0.409
0.556MetTrp: 0.556 ± 0.262
1.27MetTyr: 1.27 ± 0.261
0.0MetXaa: 0.0 ± 0.0
Asn
3.73AsnAla: 3.73 ± 0.565
0.397AsnCys: 0.397 ± 0.15
1.984AsnAsp: 1.984 ± 0.278
2.143AsnGlu: 2.143 ± 0.312
1.667AsnPhe: 1.667 ± 0.377
3.889AsnGly: 3.889 ± 0.675
1.032AsnHis: 1.032 ± 0.347
3.254AsnIle: 3.254 ± 0.672
2.699AsnLys: 2.699 ± 0.434
2.064AsnLeu: 2.064 ± 0.307
1.587AsnMet: 1.587 ± 0.395
2.143AsnAsn: 2.143 ± 0.402
2.778AsnPro: 2.778 ± 0.541
2.619AsnGln: 2.619 ± 0.683
2.222AsnArg: 2.222 ± 0.285
2.381AsnSer: 2.381 ± 0.542
2.381AsnThr: 2.381 ± 0.418
3.016AsnVal: 3.016 ± 0.603
0.556AsnTrp: 0.556 ± 0.157
0.476AsnTyr: 0.476 ± 0.173
0.0AsnXaa: 0.0 ± 0.0
Pro
4.524ProAla: 4.524 ± 0.601
0.556ProCys: 0.556 ± 0.208
2.857ProAsp: 2.857 ± 0.42
4.445ProGlu: 4.445 ± 0.954
1.349ProPhe: 1.349 ± 0.252
3.095ProGly: 3.095 ± 0.389
0.714ProHis: 0.714 ± 0.248
2.064ProIle: 2.064 ± 0.415
1.349ProLys: 1.349 ± 0.404
2.461ProLeu: 2.461 ± 0.461
0.714ProMet: 0.714 ± 0.213
2.381ProAsn: 2.381 ± 0.405
1.429ProPro: 1.429 ± 0.393
2.54ProGln: 2.54 ± 1.011
1.905ProArg: 1.905 ± 0.308
2.381ProSer: 2.381 ± 0.454
3.095ProThr: 3.095 ± 0.494
2.857ProVal: 2.857 ± 0.514
0.635ProTrp: 0.635 ± 0.191
1.905ProTyr: 1.905 ± 0.558
0.0ProXaa: 0.0 ± 0.0
Gln
5.794GlnAla: 5.794 ± 1.405
0.317GlnCys: 0.317 ± 0.149
2.302GlnAsp: 2.302 ± 0.532
2.302GlnGlu: 2.302 ± 0.439
2.381GlnPhe: 2.381 ± 0.499
3.492GlnGly: 3.492 ± 0.645
1.111GlnHis: 1.111 ± 0.262
2.064GlnIle: 2.064 ± 0.498
1.746GlnLys: 1.746 ± 0.5
4.207GlnLeu: 4.207 ± 0.534
1.429GlnMet: 1.429 ± 0.306
1.667GlnAsn: 1.667 ± 0.478
1.984GlnPro: 1.984 ± 0.983
4.365GlnGln: 4.365 ± 1.471
3.572GlnArg: 3.572 ± 0.639
2.222GlnSer: 2.222 ± 0.505
2.302GlnThr: 2.302 ± 0.545
3.175GlnVal: 3.175 ± 0.678
0.952GlnTrp: 0.952 ± 0.229
1.984GlnTyr: 1.984 ± 0.394
0.0GlnXaa: 0.0 ± 0.0
Arg
5.477ArgAla: 5.477 ± 1.047
0.556ArgCys: 0.556 ± 0.18
3.095ArgAsp: 3.095 ± 0.513
4.127ArgGlu: 4.127 ± 0.668
1.826ArgPhe: 1.826 ± 0.373
4.762ArgGly: 4.762 ± 0.497
0.873ArgHis: 0.873 ± 0.277
3.095ArgIle: 3.095 ± 0.507
2.937ArgLys: 2.937 ± 0.442
4.524ArgLeu: 4.524 ± 0.707
1.826ArgMet: 1.826 ± 0.348
2.143ArgAsn: 2.143 ± 0.426
2.064ArgPro: 2.064 ± 0.543
2.619ArgGln: 2.619 ± 0.433
4.127ArgArg: 4.127 ± 0.729
1.746ArgSer: 1.746 ± 0.321
3.334ArgThr: 3.334 ± 0.494
4.445ArgVal: 4.445 ± 0.586
0.794ArgTrp: 0.794 ± 0.267
2.222ArgTyr: 2.222 ± 0.368
0.0ArgXaa: 0.0 ± 0.0
Ser
5.873SerAla: 5.873 ± 0.561
0.079SerCys: 0.079 ± 0.077
3.175SerAsp: 3.175 ± 0.483
2.778SerGlu: 2.778 ± 0.496
1.587SerPhe: 1.587 ± 0.381
5.635SerGly: 5.635 ± 0.557
0.476SerHis: 0.476 ± 0.196
2.778SerIle: 2.778 ± 0.446
3.016SerLys: 3.016 ± 0.601
4.604SerLeu: 4.604 ± 0.557
1.587SerMet: 1.587 ± 0.438
3.651SerAsn: 3.651 ± 0.727
3.254SerPro: 3.254 ± 0.536
2.54SerGln: 2.54 ± 0.619
2.54SerArg: 2.54 ± 0.459
2.381SerSer: 2.381 ± 0.782
3.334SerThr: 3.334 ± 0.799
3.413SerVal: 3.413 ± 0.643
0.714SerTrp: 0.714 ± 0.208
2.381SerTyr: 2.381 ± 0.53
0.0SerXaa: 0.0 ± 0.0
Thr
7.62ThrAla: 7.62 ± 1.049
0.556ThrCys: 0.556 ± 0.203
3.413ThrAsp: 3.413 ± 0.507
2.699ThrGlu: 2.699 ± 0.53
2.381ThrPhe: 2.381 ± 0.471
5.635ThrGly: 5.635 ± 0.704
0.873ThrHis: 0.873 ± 0.24
2.54ThrIle: 2.54 ± 0.428
3.095ThrLys: 3.095 ± 0.451
5.556ThrLeu: 5.556 ± 0.757
1.27ThrMet: 1.27 ± 0.348
2.143ThrAsn: 2.143 ± 0.422
2.461ThrPro: 2.461 ± 0.533
3.016ThrGln: 3.016 ± 0.554
2.619ThrArg: 2.619 ± 0.316
3.572ThrSer: 3.572 ± 0.621
2.937ThrThr: 2.937 ± 0.744
5.715ThrVal: 5.715 ± 0.972
0.397ThrTrp: 0.397 ± 0.15
2.778ThrTyr: 2.778 ± 0.468
0.0ThrXaa: 0.0 ± 0.0
Val
6.429ValAla: 6.429 ± 0.728
0.635ValCys: 0.635 ± 0.229
3.889ValAsp: 3.889 ± 0.62
5.556ValGlu: 5.556 ± 0.621
2.064ValPhe: 2.064 ± 0.458
5.477ValGly: 5.477 ± 0.686
1.746ValHis: 1.746 ± 0.381
3.969ValIle: 3.969 ± 0.605
3.492ValLys: 3.492 ± 0.558
5.239ValLeu: 5.239 ± 0.669
1.508ValMet: 1.508 ± 0.324
3.572ValAsn: 3.572 ± 0.62
3.175ValPro: 3.175 ± 0.376
3.413ValGln: 3.413 ± 0.66
3.889ValArg: 3.889 ± 0.765
3.413ValSer: 3.413 ± 0.659
4.842ValThr: 4.842 ± 0.778
5.318ValVal: 5.318 ± 0.722
0.873ValTrp: 0.873 ± 0.223
3.095ValTyr: 3.095 ± 0.468
0.0ValXaa: 0.0 ± 0.0
Trp
0.873TrpAla: 0.873 ± 0.286
0.635TrpCys: 0.635 ± 0.213
1.429TrpAsp: 1.429 ± 0.24
0.714TrpGlu: 0.714 ± 0.232
0.794TrpPhe: 0.794 ± 0.262
0.952TrpGly: 0.952 ± 0.299
0.238TrpHis: 0.238 ± 0.12
0.476TrpIle: 0.476 ± 0.167
0.238TrpLys: 0.238 ± 0.148
1.905TrpLeu: 1.905 ± 0.401
0.238TrpMet: 0.238 ± 0.161
0.476TrpAsn: 0.476 ± 0.147
0.556TrpPro: 0.556 ± 0.203
0.556TrpGln: 0.556 ± 0.194
0.635TrpArg: 0.635 ± 0.226
1.111TrpSer: 1.111 ± 0.263
0.714TrpThr: 0.714 ± 0.214
0.635TrpVal: 0.635 ± 0.225
0.397TrpTrp: 0.397 ± 0.226
0.635TrpTyr: 0.635 ± 0.288
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.254TyrAla: 3.254 ± 0.552
0.317TyrCys: 0.317 ± 0.17
2.461TyrAsp: 2.461 ± 0.508
2.619TyrGlu: 2.619 ± 0.541
0.794TyrPhe: 0.794 ± 0.205
2.937TyrGly: 2.937 ± 0.395
0.873TyrHis: 0.873 ± 0.247
1.508TyrIle: 1.508 ± 0.434
1.032TyrLys: 1.032 ± 0.259
3.492TyrLeu: 3.492 ± 0.608
1.349TyrMet: 1.349 ± 0.27
1.587TyrAsn: 1.587 ± 0.291
1.667TyrPro: 1.667 ± 0.34
1.826TyrGln: 1.826 ± 0.337
2.619TyrArg: 2.619 ± 0.501
2.143TyrSer: 2.143 ± 0.417
2.778TyrThr: 2.778 ± 0.579
2.778TyrVal: 2.778 ± 0.561
0.079TyrTrp: 0.079 ± 0.069
1.349TyrTyr: 1.349 ± 0.262
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 48 proteins (12600 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski