Amino acid dipepetide frequency for Rhizopus azygosporus (Rhizopus microsporus var. azygosporus)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.331AlaAla: 5.331 ± 0.04	0.994AlaCys: 0.994 ± 0.014	3.052AlaAsp: 3.052 ± 0.025	3.86AlaGlu: 3.86 ± 0.03	2.682AlaPhe: 2.682 ± 0.021	3.155AlaGly: 3.155 ± 0.028	1.379AlaHis: 1.379 ± 0.015	4.435AlaIle: 4.435 ± 0.029	4.178AlaLys: 4.178 ± 0.03	6.257AlaLeu: 6.257 ± 0.04	1.621AlaMet: 1.621 ± 0.019	2.88AlaAsn: 2.88 ± 0.023	2.703AlaPro: 2.703 ± 0.027	2.544AlaGln: 2.544 ± 0.024	3.009AlaArg: 3.009 ± 0.023	5.271AlaSer: 5.271 ± 0.035	4.072AlaThr: 4.072 ± 0.027	4.071AlaVal: 4.071 ± 0.033	0.713AlaTrp: 0.713 ± 0.011	2.212AlaTyr: 2.212 ± 0.019	0.0AlaXaa: 0.0 ± 0.0
Cys	0.763CysAla: 0.763 ± 0.011	0.307CysCys: 0.307 ± 0.008	0.701CysAsp: 0.701 ± 0.012	0.685CysGlu: 0.685 ± 0.011	0.675CysPhe: 0.675 ± 0.011	0.856CysGly: 0.856 ± 0.016	0.409CysHis: 0.409 ± 0.009	1.039CysIle: 1.039 ± 0.015	0.792CysLys: 0.792 ± 0.011	1.498CysLeu: 1.498 ± 0.018	0.341CysMet: 0.341 ± 0.007	0.621CysAsn: 0.621 ± 0.011	0.678CysPro: 0.678 ± 0.013	0.625CysGln: 0.625 ± 0.011	0.702CysArg: 0.702 ± 0.012	1.075CysSer: 1.075 ± 0.014	0.77CysThr: 0.77 ± 0.012	0.847CysVal: 0.847 ± 0.013	0.185CysTrp: 0.185 ± 0.005	0.524CysTyr: 0.524 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	3.42AspAla: 3.42 ± 0.028	0.72AspCys: 0.72 ± 0.011	4.33AspAsp: 4.33 ± 0.045	4.625AspGlu: 4.625 ± 0.04	2.241AspPhe: 2.241 ± 0.02	2.67AspGly: 2.67 ± 0.022	1.408AspHis: 1.408 ± 0.016	4.24AspIle: 4.24 ± 0.027	3.444AspLys: 3.444 ± 0.025	5.064AspLeu: 5.064 ± 0.034	1.515AspMet: 1.515 ± 0.018	2.828AspAsn: 2.828 ± 0.025	2.599AspPro: 2.599 ± 0.022	2.301AspGln: 2.301 ± 0.02	2.47AspArg: 2.47 ± 0.021	3.716AspSer: 3.716 ± 0.036	3.314AspThr: 3.314 ± 0.023	3.357AspVal: 3.357 ± 0.028	0.731AspTrp: 0.731 ± 0.011	2.154AspTyr: 2.154 ± 0.019	0.0AspXaa: 0.0 ± 0.0
Glu	4.392GluAla: 4.392 ± 0.033	0.777GluCys: 0.777 ± 0.011	4.305GluAsp: 4.305 ± 0.031	7.066GluGlu: 7.066 ± 0.069	2.277GluPhe: 2.277 ± 0.021	2.782GluGly: 2.782 ± 0.017	1.619GluHis: 1.619 ± 0.017	3.904GluIle: 3.904 ± 0.028	5.277GluLys: 5.277 ± 0.042	6.058GluLeu: 6.058 ± 0.041	1.555GluMet: 1.555 ± 0.018	3.191GluAsn: 3.191 ± 0.025	2.382GluPro: 2.382 ± 0.021	3.554GluGln: 3.554 ± 0.03	3.598GluArg: 3.598 ± 0.029	4.415GluSer: 4.415 ± 0.033	3.546GluThr: 3.546 ± 0.027	3.187GluVal: 3.187 ± 0.034	0.763GluTrp: 0.763 ± 0.012	2.368GluTyr: 2.368 ± 0.019	0.0GluXaa: 0.0 ± 0.0
Phe	2.512PheAla: 2.512 ± 0.024	0.657PheCys: 0.657 ± 0.011	2.57PheAsp: 2.57 ± 0.02	2.488PheGlu: 2.488 ± 0.022	1.777PhePhe: 1.777 ± 0.021	2.506PheGly: 2.506 ± 0.031	0.936PheHis: 0.936 ± 0.013	2.772PheIle: 2.772 ± 0.023	2.358PheLys: 2.358 ± 0.019	3.578PheLeu: 3.578 ± 0.027	1.062PheMet: 1.062 ± 0.013	1.932PheAsn: 1.932 ± 0.02	1.588PhePro: 1.588 ± 0.016	1.494PheGln: 1.494 ± 0.016	1.687PheArg: 1.687 ± 0.016	3.026PheSer: 3.026 ± 0.027	2.31PheThr: 2.31 ± 0.02	2.581PheVal: 2.581 ± 0.023	0.553PheTrp: 0.553 ± 0.01	1.544PheTyr: 1.544 ± 0.016	0.0PheXaa: 0.0 ± 0.0
Gly	2.973GlyAla: 2.973 ± 0.028	0.765GlyCys: 0.765 ± 0.013	2.472GlyAsp: 2.472 ± 0.023	2.537GlyGlu: 2.537 ± 0.022	2.262GlyPhe: 2.262 ± 0.022	3.05GlyGly: 3.05 ± 0.03	1.407GlyHis: 1.407 ± 0.018	3.381GlyIle: 3.381 ± 0.025	3.154GlyLys: 3.154 ± 0.025	4.544GlyLeu: 4.544 ± 0.033	1.134GlyMet: 1.134 ± 0.016	2.257GlyAsn: 2.257 ± 0.02	1.783GlyPro: 1.783 ± 0.018	2.017GlyGln: 2.017 ± 0.018	2.4GlyArg: 2.4 ± 0.023	3.834GlySer: 3.834 ± 0.031	2.857GlyThr: 2.857 ± 0.023	3.095GlyVal: 3.095 ± 0.026	0.734GlyTrp: 0.734 ± 0.012	2.147GlyTyr: 2.147 ± 0.019	0.0GlyXaa: 0.0 ± 0.0
His	1.442HisAla: 1.442 ± 0.016	0.408HisCys: 0.408 ± 0.009	1.354HisAsp: 1.354 ± 0.016	1.434HisGlu: 1.434 ± 0.017	1.075HisPhe: 1.075 ± 0.012	1.293HisGly: 1.293 ± 0.013	0.992HisHis: 0.992 ± 0.015	1.665HisIle: 1.665 ± 0.015	1.385HisLys: 1.385 ± 0.014	2.409HisLeu: 2.409 ± 0.021	0.598HisMet: 0.598 ± 0.009	1.08HisAsn: 1.08 ± 0.015	1.461HisPro: 1.461 ± 0.018	1.289HisGln: 1.289 ± 0.016	1.348HisArg: 1.348 ± 0.014	1.881HisSer: 1.881 ± 0.02	1.404HisThr: 1.404 ± 0.016	1.55HisVal: 1.55 ± 0.017	0.315HisTrp: 0.315 ± 0.008	0.948HisTyr: 0.948 ± 0.012	0.0HisXaa: 0.0 ± 0.0
Ile	4.213IleAla: 4.213 ± 0.027	0.987IleCys: 0.987 ± 0.012	3.875IleAsp: 3.875 ± 0.026	4.302IleGlu: 4.302 ± 0.029	2.358IlePhe: 2.358 ± 0.025	3.093IleGly: 3.093 ± 0.025	1.779IleHis: 1.779 ± 0.018	3.866IleIle: 3.866 ± 0.028	4.213IleLys: 4.213 ± 0.028	5.738IleLeu: 5.738 ± 0.036	1.471IleMet: 1.471 ± 0.015	2.851IleAsn: 2.851 ± 0.023	3.284IlePro: 3.284 ± 0.023	3.032IleGln: 3.032 ± 0.024	3.146IleArg: 3.146 ± 0.024	4.764IleSer: 4.764 ± 0.028	3.611IleThr: 3.611 ± 0.027	3.89IleVal: 3.89 ± 0.031	0.716IleTrp: 0.716 ± 0.013	2.233IleTyr: 2.233 ± 0.019	0.0IleXaa: 0.0 ± 0.0
Lys	4.28LysAla: 4.28 ± 0.031	0.727LysCys: 0.727 ± 0.012	3.847LysAsp: 3.847 ± 0.027	5.573LysGlu: 5.573 ± 0.042	1.95LysPhe: 1.95 ± 0.018	2.849LysGly: 2.849 ± 0.023	1.653LysHis: 1.653 ± 0.017	3.552LysIle: 3.552 ± 0.023	5.986LysLys: 5.986 ± 0.053	5.713LysLeu: 5.713 ± 0.034	1.583LysMet: 1.583 ± 0.016	3.096LysAsn: 3.096 ± 0.021	2.986LysPro: 2.986 ± 0.024	3.336LysGln: 3.336 ± 0.025	4.261LysArg: 4.261 ± 0.033	4.576LysSer: 4.576 ± 0.034	3.742LysThr: 3.742 ± 0.027	3.684LysVal: 3.684 ± 0.028	0.689LysTrp: 0.689 ± 0.01	2.304LysTyr: 2.304 ± 0.021	0.0LysXaa: 0.0 ± 0.0
Leu	6.116LeuAla: 6.116 ± 0.035	1.433LeuCys: 1.433 ± 0.016	5.354LeuAsp: 5.354 ± 0.031	6.057LeuGlu: 6.057 ± 0.041	3.952LeuPhe: 3.952 ± 0.03	4.61LeuGly: 4.61 ± 0.034	2.168LeuHis: 2.168 ± 0.02	5.238LeuIle: 5.238 ± 0.035	6.172LeuLys: 6.172 ± 0.036	9.048LeuLeu: 9.048 ± 0.05	2.153LeuMet: 2.153 ± 0.022	4.352LeuAsn: 4.352 ± 0.03	4.644LeuPro: 4.644 ± 0.029	4.147LeuGln: 4.147 ± 0.03	4.509LeuArg: 4.509 ± 0.028	7.594LeuSer: 7.594 ± 0.037	5.07LeuThr: 5.07 ± 0.034	5.405LeuVal: 5.405 ± 0.034	0.981LeuTrp: 0.981 ± 0.014	3.162LeuTyr: 3.162 ± 0.023	0.0LeuXaa: 0.0 ± 0.0
Met	1.744MetAla: 1.744 ± 0.018	0.294MetCys: 0.294 ± 0.009	1.532MetAsp: 1.532 ± 0.015	1.485MetGlu: 1.485 ± 0.016	0.902MetPhe: 0.902 ± 0.012	1.128MetGly: 1.128 ± 0.014	0.531MetHis: 0.531 ± 0.009	1.52MetIle: 1.52 ± 0.016	1.572MetLys: 1.572 ± 0.014	2.13MetLeu: 2.13 ± 0.022	0.762MetMet: 0.762 ± 0.014	1.378MetAsn: 1.378 ± 0.013	1.111MetPro: 1.111 ± 0.013	0.969MetGln: 0.969 ± 0.013	1.023MetArg: 1.023 ± 0.013	2.098MetSer: 2.098 ± 0.019	1.683MetThr: 1.683 ± 0.016	1.374MetVal: 1.374 ± 0.014	0.218MetTrp: 0.218 ± 0.006	0.746MetTyr: 0.746 ± 0.01	0.0MetXaa: 0.0 ± 0.0
Asn	3.007AsnAla: 3.007 ± 0.021	0.59AsnCys: 0.59 ± 0.011	2.919AsnAsp: 2.919 ± 0.025	3.241AsnGlu: 3.241 ± 0.024	1.624AsnPhe: 1.624 ± 0.018	2.483AsnGly: 2.483 ± 0.023	1.22AsnHis: 1.22 ± 0.013	3.119AsnIle: 3.119 ± 0.025	3.181AsnLys: 3.181 ± 0.025	3.865AsnLeu: 3.865 ± 0.025	1.294AsnMet: 1.294 ± 0.016	3.447AsnAsn: 3.447 ± 0.043	2.273AsnPro: 2.273 ± 0.02	2.171AsnGln: 2.171 ± 0.02	2.238AsnArg: 2.238 ± 0.019	3.482AsnSer: 3.482 ± 0.028	3.108AsnThr: 3.108 ± 0.021	2.831AsnVal: 2.831 ± 0.024	0.557AsnTrp: 0.557 ± 0.01	1.708AsnTyr: 1.708 ± 0.018	0.0AsnXaa: 0.0 ± 0.0
Pro	2.84ProAla: 2.84 ± 0.028	0.524ProCys: 0.524 ± 0.012	2.455ProAsp: 2.455 ± 0.019	3.151ProGlu: 3.151 ± 0.023	1.99ProPhe: 1.99 ± 0.017	2.051ProGly: 2.051 ± 0.021	1.054ProHis: 1.054 ± 0.013	2.955ProIle: 2.955 ± 0.025	2.863ProLys: 2.863 ± 0.026	4.119ProLeu: 4.119 ± 0.024	1.006ProMet: 1.006 ± 0.014	2.265ProAsn: 2.265 ± 0.021	3.186ProPro: 3.186 ± 0.049	1.932ProGln: 1.932 ± 0.023	2.007ProArg: 2.007 ± 0.02	4.645ProSer: 4.645 ± 0.038	3.295ProThr: 3.295 ± 0.029	2.987ProVal: 2.987 ± 0.02	0.487ProTrp: 0.487 ± 0.009	1.618ProTyr: 1.618 ± 0.018	0.0ProXaa: 0.0 ± 0.0
Gln	2.759GlnAla: 2.759 ± 0.021	0.576GlnCys: 0.576 ± 0.011	2.416GlnAsp: 2.416 ± 0.019	3.24GlnGlu: 3.24 ± 0.028	1.69GlnPhe: 1.69 ± 0.019	1.848GlnGly: 1.848 ± 0.019	1.339GlnHis: 1.339 ± 0.018	2.396GlnIle: 2.396 ± 0.022	3.013GlnLys: 3.013 ± 0.026	4.556GlnLeu: 4.556 ± 0.029	1.106GlnMet: 1.106 ± 0.013	1.883GlnAsn: 1.883 ± 0.019	2.277GlnPro: 2.277 ± 0.028	4.348GlnGln: 4.348 ± 0.069	2.42GlnArg: 2.42 ± 0.021	3.247GlnSer: 3.247 ± 0.024	2.427GlnThr: 2.427 ± 0.022	2.629GlnVal: 2.629 ± 0.02	0.516GlnTrp: 0.516 ± 0.008	1.54GlnTyr: 1.54 ± 0.017	0.0GlnXaa: 0.0 ± 0.0
Arg	2.959ArgAla: 2.959 ± 0.023	0.655ArgCys: 0.655 ± 0.011	2.46ArgAsp: 2.46 ± 0.023	3.051ArgGlu: 3.051 ± 0.027	2.031ArgPhe: 2.031 ± 0.02	2.184ArgGly: 2.184 ± 0.021	1.455ArgHis: 1.455 ± 0.016	3.111ArgIle: 3.111 ± 0.023	3.693ArgLys: 3.693 ± 0.027	4.97ArgLeu: 4.97 ± 0.029	1.212ArgMet: 1.212 ± 0.016	2.205ArgAsn: 2.205 ± 0.02	2.28ArgPro: 2.28 ± 0.023	2.538ArgGln: 2.538 ± 0.023	3.451ArgArg: 3.451 ± 0.031	3.683ArgSer: 3.683 ± 0.03	2.619ArgThr: 2.619 ± 0.021	2.721ArgVal: 2.721 ± 0.02	0.617ArgTrp: 0.617 ± 0.01	1.803ArgTyr: 1.803 ± 0.018	0.0ArgXaa: 0.0 ± 0.0
Ser	4.704SerAla: 4.704 ± 0.031	1.049SerCys: 1.049 ± 0.014	4.055SerAsp: 4.055 ± 0.031	4.11SerGlu: 4.11 ± 0.028	3.372SerPhe: 3.372 ± 0.024	3.69SerGly: 3.69 ± 0.029	1.838SerHis: 1.838 ± 0.02	5.316SerIle: 5.316 ± 0.031	4.811SerLys: 4.811 ± 0.035	7.243SerLeu: 7.243 ± 0.037	1.836SerMet: 1.836 ± 0.017	4.103SerAsn: 4.103 ± 0.032	3.966SerPro: 3.966 ± 0.035	3.184SerGln: 3.184 ± 0.025	3.744SerArg: 3.744 ± 0.028	8.734SerSer: 8.734 ± 0.078	5.518SerThr: 5.518 ± 0.04	4.578SerVal: 4.578 ± 0.032	0.83SerTrp: 0.83 ± 0.013	2.384SerTyr: 2.384 ± 0.023	0.0SerXaa: 0.0 ± 0.0
Thr	4.132ThrAla: 4.132 ± 0.026	0.873ThrCys: 0.873 ± 0.013	3.005ThrAsp: 3.005 ± 0.024	3.585ThrGlu: 3.585 ± 0.031	2.476ThrPhe: 2.476 ± 0.019	3.118ThrGly: 3.118 ± 0.029	1.359ThrHis: 1.359 ± 0.016	4.038ThrIle: 4.038 ± 0.03	3.576ThrLys: 3.576 ± 0.028	5.542ThrLeu: 5.542 ± 0.031	1.351ThrMet: 1.351 ± 0.012	2.777ThrAsn: 2.777 ± 0.025	3.264ThrPro: 3.264 ± 0.03	2.183ThrGln: 2.183 ± 0.019	2.635ThrArg: 2.635 ± 0.017	5.21ThrSer: 5.21 ± 0.041	4.776ThrThr: 4.776 ± 0.051	3.91ThrVal: 3.91 ± 0.03	0.658ThrTrp: 0.658 ± 0.012	1.842ThrTyr: 1.842 ± 0.02	0.0ThrXaa: 0.0 ± 0.0
Val	3.999ValAla: 3.999 ± 0.032	0.92ValCys: 0.92 ± 0.013	3.654ValAsp: 3.654 ± 0.024	3.755ValGlu: 3.755 ± 0.032	2.458ValPhe: 2.458 ± 0.025	2.935ValGly: 2.935 ± 0.026	1.499ValHis: 1.499 ± 0.014	3.865ValIle: 3.865 ± 0.029	3.758ValLys: 3.758 ± 0.03	5.405ValLeu: 5.405 ± 0.031	1.415ValMet: 1.415 ± 0.015	2.809ValAsn: 2.809 ± 0.025	2.924ValPro: 2.924 ± 0.021	2.507ValGln: 2.507 ± 0.019	2.652ValArg: 2.652 ± 0.02	4.376ValSer: 4.376 ± 0.029	3.48ValThr: 3.48 ± 0.027	3.799ValVal: 3.799 ± 0.034	0.688ValTrp: 0.688 ± 0.011	2.16ValTyr: 2.16 ± 0.019	0.0ValXaa: 0.0 ± 0.0
Trp	0.669TrpAla: 0.669 ± 0.01	0.188TrpCys: 0.188 ± 0.006	0.692TrpAsp: 0.692 ± 0.013	0.65TrpGlu: 0.65 ± 0.011	0.522TrpPhe: 0.522 ± 0.011	0.552TrpGly: 0.552 ± 0.012	0.275TrpHis: 0.275 ± 0.007	0.831TrpIle: 0.831 ± 0.013	0.878TrpLys: 0.878 ± 0.014	1.062TrpLeu: 1.062 ± 0.014	0.325TrpMet: 0.325 ± 0.008	0.645TrpAsn: 0.645 ± 0.012	0.41TrpPro: 0.41 ± 0.008	0.456TrpGln: 0.456 ± 0.01	0.597TrpArg: 0.597 ± 0.01	0.918TrpSer: 0.918 ± 0.011	0.732TrpThr: 0.732 ± 0.011	0.61TrpVal: 0.61 ± 0.012	0.192TrpTrp: 0.192 ± 0.006	0.396TrpTyr: 0.396 ± 0.009	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.16TyrAla: 2.16 ± 0.02	0.648TyrCys: 0.648 ± 0.011	2.1TyrAsp: 2.1 ± 0.019	2.12TyrGlu: 2.12 ± 0.022	1.616TyrPhe: 1.616 ± 0.016	1.95TyrGly: 1.95 ± 0.019	1.009TyrHis: 1.009 ± 0.013	2.179TyrIle: 2.179 ± 0.017	2.085TyrLys: 2.085 ± 0.019	3.367TyrLeu: 3.367 ± 0.024	0.863TyrMet: 0.863 ± 0.011	1.755TyrAsn: 1.755 ± 0.017	1.634TyrPro: 1.634 ± 0.019	1.601TyrGln: 1.601 ± 0.017	1.802TyrArg: 1.802 ± 0.021	2.518TyrSer: 2.518 ± 0.022	1.975TyrThr: 1.975 ± 0.019	1.954TyrVal: 1.954 ± 0.019	0.457TyrTrp: 0.457 ± 0.01	1.551TyrTyr: 1.551 ± 0.016	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 15771 proteins (6121853 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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