Thauera sp. 27

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Rhodocyclales; Zoogloeaceae; Thauera; unclassified Thauera

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4268 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|N6XJ09|N6XJ09_9RHOO Peptidase U62 modulator of DNA gyrase OS=Thauera sp. 27 OX=305700 GN=B447_07377 PE=3 SV=1
MM1 pKa = 6.98SAEE4 pKa = 4.13VEE6 pKa = 4.39TPDD9 pKa = 3.79ILVFTDD15 pKa = 3.61SAANKK20 pKa = 8.85VRR22 pKa = 11.84EE23 pKa = 4.42LIEE26 pKa = 4.53EE27 pKa = 4.19EE28 pKa = 4.67GNPQLKK34 pKa = 10.31LRR36 pKa = 11.84VFVSGGGCSGFQYY49 pKa = 10.93GFTFDD54 pKa = 4.28EE55 pKa = 4.93EE56 pKa = 4.59VNEE59 pKa = 5.57DD60 pKa = 3.3DD61 pKa = 3.6TTYY64 pKa = 11.03EE65 pKa = 4.12KK66 pKa = 11.07NGVMLLIDD74 pKa = 3.88AMSYY78 pKa = 10.46QYY80 pKa = 11.17LIGAEE85 pKa = 3.66IDD87 pKa = 3.63YY88 pKa = 10.7TEE90 pKa = 4.43GLEE93 pKa = 4.25GSQFVIRR100 pKa = 11.84NPNATSTCGCGSSFSAA116 pKa = 4.79

Molecular weight: 12.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|N6YET6|N6YET6_9RHOO Uncharacterized protein OS=Thauera sp. 27 OX=305700 GN=B447_14164 PE=4 SV=1
MM1 pKa = 7.22NGKK4 pKa = 8.98HH5 pKa = 6.29PAQAPHH11 pKa = 6.56APVVGAGRR19 pKa = 11.84RR20 pKa = 11.84SLQHH24 pKa = 6.51RR25 pKa = 11.84LGALRR30 pKa = 11.84AGLKK34 pKa = 9.99AALRR38 pKa = 11.84ALRR41 pKa = 11.84SHH43 pKa = 7.78PIKK46 pKa = 10.68HH47 pKa = 5.67PQRR50 pKa = 5.12

Molecular weight: 5.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4268

0

4268

1408651

24

11953

330.0

35.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.036 ± 0.061

0.958 ± 0.015

5.545 ± 0.03

5.871 ± 0.034

3.521 ± 0.024

8.41 ± 0.037

2.303 ± 0.018

4.584 ± 0.028

2.8 ± 0.035

10.938 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.361 ± 0.027

2.493 ± 0.024

4.977 ± 0.026

3.455 ± 0.02

7.597 ± 0.04

5.09 ± 0.034

4.83 ± 0.032

7.647 ± 0.039

1.404 ± 0.015

2.179 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski