Sphingopyxis sp. Root1497

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingopyxis; unclassified Sphingopyxis

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4384 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q7YLG1|A0A0Q7YLG1_9SPHN Cytochrome OS=Sphingopyxis sp. Root1497 OX=1736474 GN=ASD67_12080 PE=3 SV=1
MM1 pKa = 7.05VLSTRR6 pKa = 11.84LQTVGEE12 pKa = 4.28SGNVFIDD19 pKa = 3.36TLVYY23 pKa = 10.4GVAFRR28 pKa = 11.84PATTVIYY35 pKa = 10.32ALQGNPGDD43 pKa = 3.66TGLNGGALWAANGAAGAYY61 pKa = 8.9AAALASWAAVANIRR75 pKa = 11.84FQAATTAYY83 pKa = 10.18DD84 pKa = 3.74GTGSRR89 pKa = 11.84ASYY92 pKa = 11.09DD93 pKa = 3.34FVGQLSTSLPSDD105 pKa = 3.29VLGQHH110 pKa = 6.31TLPTTGDD117 pKa = 3.15MVGQFNTTISYY128 pKa = 7.45FTTASNQAGGLSFLTFVHH146 pKa = 6.29EE147 pKa = 4.48LGHH150 pKa = 6.94GIGLLHH156 pKa = 6.58PHH158 pKa = 6.94ADD160 pKa = 3.72EE161 pKa = 5.56PGDD164 pKa = 3.99DD165 pKa = 4.05SFPGLGDD172 pKa = 3.72AFSTGPFGLNQGIFTTMTYY191 pKa = 10.8NDD193 pKa = 4.11GYY195 pKa = 10.03EE196 pKa = 4.15DD197 pKa = 3.54VGRR200 pKa = 11.84SPSVNYY206 pKa = 8.8GWQMGPGAFDD216 pKa = 3.15IAAVQFLYY224 pKa = 10.7GANNSTGAGNTTYY237 pKa = 11.03TMPTLNQAGTGWMTIWDD254 pKa = 3.85VGGTDD259 pKa = 3.78TISAQGSQLDD269 pKa = 4.03AIIDD273 pKa = 3.65LRR275 pKa = 11.84AATLRR280 pKa = 11.84NEE282 pKa = 4.07EE283 pKa = 4.14GGGGFVSRR291 pKa = 11.84NGGVLGGFTIANGAVIEE308 pKa = 4.08NAIGGNGDD316 pKa = 3.88DD317 pKa = 4.64LLVGNAANNSLNGGAGWDD335 pKa = 3.86TVSYY339 pKa = 11.36AHH341 pKa = 6.29MTSAVTVNLATGIATGEE358 pKa = 4.3GNDD361 pKa = 3.57TLSAIEE367 pKa = 3.95RR368 pKa = 11.84AIGGSGDD375 pKa = 3.88DD376 pKa = 3.93VMTAFIGASIVNGSQDD392 pKa = 3.8VIKK395 pKa = 10.8SAALRR400 pKa = 11.84NGEE403 pKa = 4.06IASALSLDD411 pKa = 4.15GRR413 pKa = 11.84FSPHH417 pKa = 6.54SSDD420 pKa = 3.94VSIQAVGAGVNSVTVHH436 pKa = 6.42GEE438 pKa = 3.67GGLQRR443 pKa = 11.84DD444 pKa = 4.53VYY446 pKa = 11.3SFTLTAGAQITIDD459 pKa = 3.22IDD461 pKa = 3.23NSFRR465 pKa = 11.84MDD467 pKa = 4.09SIIALFGPGLYY478 pKa = 10.47GPSSLMTTNDD488 pKa = 4.07DD489 pKa = 3.93SPNDD493 pKa = 3.94LDD495 pKa = 3.89VGSANSLDD503 pKa = 3.68SFLTFNATTAGTYY516 pKa = 10.02YY517 pKa = 11.35VMVSTYY523 pKa = 10.19SAEE526 pKa = 4.14SDD528 pKa = 3.33GGGPIALGSSYY539 pKa = 10.57TLNISAANTVAAAGTILLGSTLDD562 pKa = 3.74GGAGNDD568 pKa = 4.0TLNGGAGIDD577 pKa = 3.73TLIGGIGNDD586 pKa = 3.55MLNGGGGADD595 pKa = 3.86LMYY598 pKa = 10.97GGTGNDD604 pKa = 3.26NYY606 pKa = 10.84VIDD609 pKa = 4.36AQGDD613 pKa = 3.87LTFEE617 pKa = 4.33GLNGGTDD624 pKa = 3.26TVTASVGHH632 pKa = 5.55YY633 pKa = 10.35LYY635 pKa = 11.29ANLEE639 pKa = 4.1NLTLATGAGDD649 pKa = 3.14IFGVGNEE656 pKa = 4.68LANTLQGNAGANLLLGGLGDD676 pKa = 3.86DD677 pKa = 4.87VIRR680 pKa = 11.84GGAGVDD686 pKa = 3.61SLFGEE691 pKa = 4.58AGVDD695 pKa = 3.77QLFGDD700 pKa = 3.94AGIDD704 pKa = 3.67YY705 pKa = 10.82LVGGLGNDD713 pKa = 3.55ILDD716 pKa = 4.2GGADD720 pKa = 3.46ADD722 pKa = 3.93ALYY725 pKa = 11.17GEE727 pKa = 5.66DD728 pKa = 5.55GDD730 pKa = 4.24DD731 pKa = 3.72TLIGGAGFFTDD742 pKa = 3.79ILVGGAGNDD751 pKa = 3.31ILRR754 pKa = 11.84GASGLGDD761 pKa = 4.2YY762 pKa = 11.48DD763 pKa = 5.77LMDD766 pKa = 4.59GGAGNDD772 pKa = 3.02IYY774 pKa = 11.66YY775 pKa = 10.51VDD777 pKa = 4.51TPDD780 pKa = 4.83DD781 pKa = 3.77LTFEE785 pKa = 4.39AVGGGADD792 pKa = 2.97TVYY795 pKa = 11.49ANINGAGYY803 pKa = 9.84YY804 pKa = 10.35LYY806 pKa = 11.15ANVEE810 pKa = 4.15NLVLEE815 pKa = 4.69GNTPFGVGNDD825 pKa = 3.41LANRR829 pKa = 11.84LTGNAIGNYY838 pKa = 9.89LLGGRR843 pKa = 11.84GNDD846 pKa = 3.47TLNGKK851 pKa = 9.26GGNDD855 pKa = 3.28VLFGEE860 pKa = 5.0GGVDD864 pKa = 2.93TFVFEE869 pKa = 4.81RR870 pKa = 11.84GTGGDD875 pKa = 3.59VIGDD879 pKa = 4.09FARR882 pKa = 11.84GTDD885 pKa = 4.06KK886 pKa = 10.93IDD888 pKa = 3.14VSAFGFASFAALQAGFTQVGANGAINLGNGDD919 pKa = 4.95FIVLHH924 pKa = 6.05NVTMSQLTQGDD935 pKa = 4.93FILSATAGKK944 pKa = 9.44PDD946 pKa = 4.32LFGDD950 pKa = 3.96GAKK953 pKa = 10.24AVEE956 pKa = 4.47DD957 pKa = 3.79AGFAPDD963 pKa = 4.11AGTPVLDD970 pKa = 3.38WHH972 pKa = 7.14PDD974 pKa = 2.57HH975 pKa = 7.41WMAQYY980 pKa = 8.26HH981 pKa = 5.14TAYY984 pKa = 10.69QLGFVV989 pKa = 4.32

Molecular weight: 99.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q7YY40|A0A0Q7YY40_9SPHN Pimeloyl-CoA dehydrogenase large subunit OS=Sphingopyxis sp. Root1497 OX=1736474 GN=ASD67_22015 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.43GFRR19 pKa = 11.84ARR21 pKa = 11.84KK22 pKa = 7.81ATVGGRR28 pKa = 11.84KK29 pKa = 8.83ILANRR34 pKa = 11.84RR35 pKa = 11.84AQGRR39 pKa = 11.84KK40 pKa = 9.26KK41 pKa = 10.65LSAA44 pKa = 3.91

Molecular weight: 5.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4384

0

4384

1426498

29

4039

325.4

35.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.824 ± 0.064

0.757 ± 0.01

6.305 ± 0.027

5.121 ± 0.03

3.614 ± 0.027

9.205 ± 0.054

1.949 ± 0.019

5.106 ± 0.023

3.014 ± 0.033

9.679 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.409 ± 0.02

2.557 ± 0.029

5.326 ± 0.029

2.906 ± 0.021

7.004 ± 0.035

5.121 ± 0.028

5.273 ± 0.032

7.051 ± 0.028

1.497 ± 0.017

2.279 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski