Amino acid dipepetide frequency for Gemmobacter megaterium

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.589AlaAla: 19.589 ± 0.181
1.163AlaCys: 1.163 ± 0.034
7.747AlaAsp: 7.747 ± 0.084
8.46AlaGlu: 8.46 ± 0.111
4.331AlaPhe: 4.331 ± 0.065
11.824AlaGly: 11.824 ± 0.123
2.514AlaHis: 2.514 ± 0.046
5.822AlaIle: 5.822 ± 0.082
3.041AlaLys: 3.041 ± 0.06
15.239AlaLeu: 15.239 ± 0.155
4.302AlaMet: 4.302 ± 0.063
2.572AlaAsn: 2.572 ± 0.049
6.814AlaPro: 6.814 ± 0.091
4.861AlaGln: 4.861 ± 0.07
10.842AlaArg: 10.842 ± 0.124
5.5AlaSer: 5.5 ± 0.083
6.5AlaThr: 6.5 ± 0.079
9.233AlaVal: 9.233 ± 0.097
1.639AlaTrp: 1.639 ± 0.037
2.375AlaTyr: 2.375 ± 0.043
0.0AlaXaa: 0.0 ± 0.0
Cys
1.092CysAla: 1.092 ± 0.032
0.112CysCys: 0.112 ± 0.009
0.562CysAsp: 0.562 ± 0.019
0.361CysGlu: 0.361 ± 0.018
0.312CysPhe: 0.312 ± 0.016
0.885CysGly: 0.885 ± 0.029
0.283CysHis: 0.283 ± 0.016
0.382CysIle: 0.382 ± 0.017
0.156CysLys: 0.156 ± 0.012
0.839CysLeu: 0.839 ± 0.023
0.143CysMet: 0.143 ± 0.011
0.216CysAsn: 0.216 ± 0.015
0.52CysPro: 0.52 ± 0.022
0.233CysGln: 0.233 ± 0.015
0.577CysArg: 0.577 ± 0.023
0.369CysSer: 0.369 ± 0.017
0.435CysThr: 0.435 ± 0.018
0.553CysVal: 0.553 ± 0.021
0.12CysTrp: 0.12 ± 0.01
0.179CysTyr: 0.179 ± 0.012
0.0CysXaa: 0.0 ± 0.0
Asp
6.855AspAla: 6.855 ± 0.083
0.489AspCys: 0.489 ± 0.02
3.085AspAsp: 3.085 ± 0.068
2.957AspGlu: 2.957 ± 0.06
2.088AspPhe: 2.088 ± 0.043
5.681AspGly: 5.681 ± 0.09
1.392AspHis: 1.392 ± 0.039
2.679AspIle: 2.679 ± 0.051
1.39AspLys: 1.39 ± 0.036
6.901AspLeu: 6.901 ± 0.086
1.678AspMet: 1.678 ± 0.038
0.993AspAsn: 0.993 ± 0.029
4.085AspPro: 4.085 ± 0.057
1.875AspGln: 1.875 ± 0.039
5.026AspArg: 5.026 ± 0.07
2.139AspSer: 2.139 ± 0.041
2.809AspThr: 2.809 ± 0.065
3.87AspVal: 3.87 ± 0.057
1.257AspTrp: 1.257 ± 0.036
1.372AspTyr: 1.372 ± 0.033
0.0AspXaa: 0.0 ± 0.0
Glu
7.858GluAla: 7.858 ± 0.099
0.306GluCys: 0.306 ± 0.015
2.885GluAsp: 2.885 ± 0.05
2.365GluGlu: 2.365 ± 0.051
1.628GluPhe: 1.628 ± 0.039
4.347GluGly: 4.347 ± 0.065
0.978GluHis: 0.978 ± 0.026
3.15GluIle: 3.15 ± 0.051
1.467GluLys: 1.467 ± 0.039
4.618GluLeu: 4.618 ± 0.073
1.51GluMet: 1.51 ± 0.035
1.243GluAsn: 1.243 ± 0.033
2.548GluPro: 2.548 ± 0.11
1.679GluGln: 1.679 ± 0.039
4.19GluArg: 4.19 ± 0.063
1.988GluSer: 1.988 ± 0.039
3.518GluThr: 3.518 ± 0.054
4.126GluVal: 4.126 ± 0.069
0.667GluTrp: 0.667 ± 0.026
0.872GluTyr: 0.872 ± 0.025
0.0GluXaa: 0.0 ± 0.0
Phe
4.565PheAla: 4.565 ± 0.071
0.36PheCys: 0.36 ± 0.018
2.689PheAsp: 2.689 ± 0.052
1.768PheGlu: 1.768 ± 0.037
1.221PhePhe: 1.221 ± 0.034
3.778PheGly: 3.778 ± 0.063
0.778PheHis: 0.778 ± 0.024
1.404PheIle: 1.404 ± 0.036
0.737PheLys: 0.737 ± 0.027
3.414PheLeu: 3.414 ± 0.063
0.75PheMet: 0.75 ± 0.024
0.841PheAsn: 0.841 ± 0.029
1.61PhePro: 1.61 ± 0.036
0.967PheGln: 0.967 ± 0.029
2.352PheArg: 2.352 ± 0.046
1.879PheSer: 1.879 ± 0.037
1.894PheThr: 1.894 ± 0.042
2.544PheVal: 2.544 ± 0.048
0.579PheTrp: 0.579 ± 0.024
0.773PheTyr: 0.773 ± 0.028
0.0PheXaa: 0.0 ± 0.0
Gly
11.188GlyAla: 11.188 ± 0.116
0.864GlyCys: 0.864 ± 0.028
4.699GlyAsp: 4.699 ± 0.09
4.139GlyGlu: 4.139 ± 0.064
3.644GlyPhe: 3.644 ± 0.059
8.114GlyGly: 8.114 ± 0.145
2.126GlyHis: 2.126 ± 0.048
4.479GlyIle: 4.479 ± 0.067
2.869GlyLys: 2.869 ± 0.056
10.24GlyLeu: 10.24 ± 0.109
2.754GlyMet: 2.754 ± 0.043
2.202GlyAsn: 2.202 ± 0.071
4.139GlyPro: 4.139 ± 0.063
3.44GlyGln: 3.44 ± 0.061
6.675GlyArg: 6.675 ± 0.083
4.018GlySer: 4.018 ± 0.064
4.865GlyThr: 4.865 ± 0.084
6.506GlyVal: 6.506 ± 0.069
1.789GlyTrp: 1.789 ± 0.039
2.176GlyTyr: 2.176 ± 0.042
0.0GlyXaa: 0.0 ± 0.0
His
2.494HisAla: 2.494 ± 0.043
0.234HisCys: 0.234 ± 0.016
1.33HisAsp: 1.33 ± 0.037
0.956HisGlu: 0.956 ± 0.03
0.747HisPhe: 0.747 ± 0.024
2.114HisGly: 2.114 ± 0.049
0.603HisHis: 0.603 ± 0.022
0.925HisIle: 0.925 ± 0.029
0.444HisLys: 0.444 ± 0.019
2.275HisLeu: 2.275 ± 0.048
0.51HisMet: 0.51 ± 0.022
0.41HisAsn: 0.41 ± 0.017
1.509HisPro: 1.509 ± 0.036
0.619HisGln: 0.619 ± 0.023
1.542HisArg: 1.542 ± 0.038
0.927HisSer: 0.927 ± 0.031
0.796HisThr: 0.796 ± 0.025
1.621HisVal: 1.621 ± 0.039
0.39HisTrp: 0.39 ± 0.019
0.514HisTyr: 0.514 ± 0.019
0.0HisXaa: 0.0 ± 0.0
Ile
7.143IleAla: 7.143 ± 0.085
0.568IleCys: 0.568 ± 0.022
3.072IleAsp: 3.072 ± 0.047
2.942IleGlu: 2.942 ± 0.055
1.542IlePhe: 1.542 ± 0.038
4.823IleGly: 4.823 ± 0.057
0.911IleHis: 0.911 ± 0.025
1.837IleIle: 1.837 ± 0.044
1.008IleLys: 1.008 ± 0.031
4.638IleLeu: 4.638 ± 0.072
0.942IleMet: 0.942 ± 0.032
1.107IleAsn: 1.107 ± 0.033
2.276IlePro: 2.276 ± 0.043
1.098IleGln: 1.098 ± 0.033
3.496IleArg: 3.496 ± 0.051
2.569IleSer: 2.569 ± 0.048
2.654IleThr: 2.654 ± 0.046
3.725IleVal: 3.725 ± 0.06
0.701IleTrp: 0.701 ± 0.026
1.033IleTyr: 1.033 ± 0.031
0.0IleXaa: 0.0 ± 0.0
Lys
3.568LysAla: 3.568 ± 0.071
0.131LysCys: 0.131 ± 0.01
1.378LysAsp: 1.378 ± 0.032
1.122LysGlu: 1.122 ± 0.036
0.727LysPhe: 0.727 ± 0.027
2.483LysGly: 2.483 ± 0.051
0.458LysHis: 0.458 ± 0.019
1.168LysIle: 1.168 ± 0.034
0.812LysLys: 0.812 ± 0.03
2.33LysLeu: 2.33 ± 0.043
0.635LysMet: 0.635 ± 0.025
0.542LysAsn: 0.542 ± 0.022
1.571LysPro: 1.571 ± 0.041
0.672LysGln: 0.672 ± 0.024
1.79LysArg: 1.79 ± 0.041
1.363LysSer: 1.363 ± 0.036
1.453LysThr: 1.453 ± 0.036
2.105LysVal: 2.105 ± 0.047
0.303LysTrp: 0.303 ± 0.016
0.492LysTyr: 0.492 ± 0.021
0.0LysXaa: 0.0 ± 0.0
Leu
15.544LeuAla: 15.544 ± 0.142
0.864LeuCys: 0.864 ± 0.028
6.276LeuAsp: 6.276 ± 0.067
4.9LeuGlu: 4.9 ± 0.057
3.338LeuPhe: 3.338 ± 0.064
9.028LeuGly: 9.028 ± 0.097
2.126LeuHis: 2.126 ± 0.048
5.178LeuIle: 5.178 ± 0.079
2.684LeuLys: 2.684 ± 0.056
9.416LeuLeu: 9.416 ± 0.142
2.905LeuMet: 2.905 ± 0.061
2.499LeuAsn: 2.499 ± 0.045
6.282LeuPro: 6.282 ± 0.092
2.528LeuGln: 2.528 ± 0.04
8.267LeuArg: 8.267 ± 0.099
6.284LeuSer: 6.284 ± 0.075
6.135LeuThr: 6.135 ± 0.079
7.517LeuVal: 7.517 ± 0.103
1.42LeuTrp: 1.42 ± 0.038
1.903LeuTyr: 1.903 ± 0.044
0.0LeuXaa: 0.0 ± 0.0
Met
3.896MetAla: 3.896 ± 0.064
0.151MetCys: 0.151 ± 0.01
1.366MetAsp: 1.366 ± 0.038
1.244MetGlu: 1.244 ± 0.034
0.82MetPhe: 0.82 ± 0.029
2.357MetGly: 2.357 ± 0.048
0.438MetHis: 0.438 ± 0.018
1.499MetIle: 1.499 ± 0.031
0.868MetLys: 0.868 ± 0.028
2.795MetLeu: 2.795 ± 0.05
0.781MetMet: 0.781 ± 0.024
0.739MetAsn: 0.739 ± 0.026
1.605MetPro: 1.605 ± 0.038
1.005MetGln: 1.005 ± 0.028
2.03MetArg: 2.03 ± 0.041
1.508MetSer: 1.508 ± 0.033
2.201MetThr: 2.201 ± 0.044
2.049MetVal: 2.049 ± 0.037
0.24MetTrp: 0.24 ± 0.015
0.347MetTyr: 0.347 ± 0.015
0.0MetXaa: 0.0 ± 0.0
Asn
2.88AsnAla: 2.88 ± 0.054
0.196AsnCys: 0.196 ± 0.013
1.307AsnAsp: 1.307 ± 0.059
0.86AsnGlu: 0.86 ± 0.031
0.777AsnPhe: 0.777 ± 0.025
2.137AsnGly: 2.137 ± 0.05
0.454AsnHis: 0.454 ± 0.018
1.142AsnIle: 1.142 ± 0.031
0.472AsnLys: 0.472 ± 0.018
2.33AsnLeu: 2.33 ± 0.047
0.599AsnMet: 0.599 ± 0.023
0.489AsnAsn: 0.489 ± 0.02
1.732AsnPro: 1.732 ± 0.039
0.579AsnGln: 0.579 ± 0.023
1.795AsnArg: 1.795 ± 0.037
0.952AsnSer: 0.952 ± 0.031
1.156AsnThr: 1.156 ± 0.03
1.534AsnVal: 1.534 ± 0.041
0.348AsnTrp: 0.348 ± 0.018
0.502AsnTyr: 0.502 ± 0.023
0.0AsnXaa: 0.0 ± 0.0
Pro
7.087ProAla: 7.087 ± 0.092
0.375ProCys: 0.375 ± 0.018
4.304ProAsp: 4.304 ± 0.067
4.05ProGlu: 4.05 ± 0.062
1.97ProPhe: 1.97 ± 0.039
5.425ProGly: 5.425 ± 0.072
1.183ProHis: 1.183 ± 0.035
2.001ProIle: 2.001 ± 0.044
1.35ProLys: 1.35 ± 0.034
5.072ProLeu: 5.072 ± 0.066
1.504ProMet: 1.504 ± 0.036
1.108ProAsn: 1.108 ± 0.032
3.004ProPro: 3.004 ± 0.072
1.941ProGln: 1.941 ± 0.037
3.436ProArg: 3.436 ± 0.056
2.299ProSer: 2.299 ± 0.041
2.524ProThr: 2.524 ± 0.133
4.979ProVal: 4.979 ± 0.07
0.762ProTrp: 0.762 ± 0.027
1.03ProTyr: 1.03 ± 0.032
0.0ProXaa: 0.0 ± 0.0
Gln
4.582GlnAla: 4.582 ± 0.074
0.176GlnCys: 0.176 ± 0.012
1.721GlnAsp: 1.721 ± 0.039
1.332GlnGlu: 1.332 ± 0.036
0.987GlnPhe: 0.987 ± 0.026
3.15GlnGly: 3.15 ± 0.049
0.589GlnHis: 0.589 ± 0.023
1.978GlnIle: 1.978 ± 0.043
0.859GlnLys: 0.859 ± 0.028
2.589GlnLeu: 2.589 ± 0.055
1.079GlnMet: 1.079 ± 0.028
0.741GlnAsn: 0.741 ± 0.028
1.826GlnPro: 1.826 ± 0.043
1.018GlnGln: 1.018 ± 0.032
2.326GlnArg: 2.326 ± 0.041
1.566GlnSer: 1.566 ± 0.037
1.692GlnThr: 1.692 ± 0.038
2.552GlnVal: 2.552 ± 0.046
0.404GlnTrp: 0.404 ± 0.019
0.5GlnTyr: 0.5 ± 0.022
0.0GlnXaa: 0.0 ± 0.0
Arg
9.661ArgAla: 9.661 ± 0.108
0.503ArgCys: 0.503 ± 0.02
4.416ArgAsp: 4.416 ± 0.062
3.559ArgGlu: 3.559 ± 0.061
2.893ArgPhe: 2.893 ± 0.047
5.186ArgGly: 5.186 ± 0.073
1.842ArgHis: 1.842 ± 0.041
4.358ArgIle: 4.358 ± 0.068
2.151ArgLys: 2.151 ± 0.051
9.102ArgLeu: 9.102 ± 0.109
2.307ArgMet: 2.307 ± 0.04
1.728ArgAsn: 1.728 ± 0.032
4.017ArgPro: 4.017 ± 0.074
2.622ArgGln: 2.622 ± 0.051
5.875ArgArg: 5.875 ± 0.09
3.372ArgSer: 3.372 ± 0.058
3.283ArgThr: 3.283 ± 0.051
5.204ArgVal: 5.204 ± 0.063
1.1ArgTrp: 1.1 ± 0.032
1.555ArgTyr: 1.555 ± 0.036
0.0ArgXaa: 0.0 ± 0.0
Ser
5.808SerAla: 5.808 ± 0.072
0.41SerCys: 0.41 ± 0.022
2.755SerAsp: 2.755 ± 0.047
2.218SerGlu: 2.218 ± 0.044
1.94SerPhe: 1.94 ± 0.039
5.411SerGly: 5.411 ± 0.07
1.02SerHis: 1.02 ± 0.033
2.059SerIle: 2.059 ± 0.043
1.05SerLys: 1.05 ± 0.033
4.9SerLeu: 4.9 ± 0.07
1.198SerMet: 1.198 ± 0.033
1.034SerAsn: 1.034 ± 0.033
2.501SerPro: 2.501 ± 0.052
1.389SerGln: 1.389 ± 0.031
3.416SerArg: 3.416 ± 0.051
2.177SerSer: 2.177 ± 0.048
2.261SerThr: 2.261 ± 0.044
3.655SerVal: 3.655 ± 0.054
0.692SerTrp: 0.692 ± 0.023
1.136SerTyr: 1.136 ± 0.033
0.0SerXaa: 0.0 ± 0.0
Thr
6.445ThrAla: 6.445 ± 0.077
0.461ThrCys: 0.461 ± 0.02
2.978ThrAsp: 2.978 ± 0.055
2.919ThrGlu: 2.919 ± 0.109
1.857ThrPhe: 1.857 ± 0.037
5.684ThrGly: 5.684 ± 0.072
1.066ThrHis: 1.066 ± 0.033
2.588ThrIle: 2.588 ± 0.042
1.082ThrLys: 1.082 ± 0.032
6.105ThrLeu: 6.105 ± 0.072
1.291ThrMet: 1.291 ± 0.033
1.14ThrAsn: 1.14 ± 0.03
3.5ThrPro: 3.5 ± 0.058
1.415ThrGln: 1.415 ± 0.038
3.682ThrArg: 3.682 ± 0.054
2.482ThrSer: 2.482 ± 0.049
2.83ThrThr: 2.83 ± 0.052
4.196ThrVal: 4.196 ± 0.067
0.712ThrTrp: 0.712 ± 0.028
1.084ThrTyr: 1.084 ± 0.027
0.0ThrXaa: 0.0 ± 0.0
Val
10.098ValAla: 10.098 ± 0.112
0.612ValCys: 0.612 ± 0.021
3.817ValAsp: 3.817 ± 0.064
4.193ValGlu: 4.193 ± 0.066
2.748ValPhe: 2.748 ± 0.05
5.378ValGly: 5.378 ± 0.08
1.362ValHis: 1.362 ± 0.03
3.97ValIle: 3.97 ± 0.062
1.808ValLys: 1.808 ± 0.041
8.393ValLeu: 8.393 ± 0.098
2.186ValMet: 2.186 ± 0.041
1.759ValAsn: 1.759 ± 0.039
4.073ValPro: 4.073 ± 0.061
2.415ValGln: 2.415 ± 0.045
4.624ValArg: 4.624 ± 0.063
3.807ValSer: 3.807 ± 0.053
4.657ValThr: 4.657 ± 0.075
5.954ValVal: 5.954 ± 0.087
1.113ValTrp: 1.113 ± 0.03
1.356ValTyr: 1.356 ± 0.036
0.0ValXaa: 0.0 ± 0.0
Trp
1.544TrpAla: 1.544 ± 0.041
0.139TrpCys: 0.139 ± 0.012
0.778TrpAsp: 0.778 ± 0.025
0.555TrpGlu: 0.555 ± 0.023
0.594TrpPhe: 0.594 ± 0.024
1.114TrpGly: 1.114 ± 0.036
0.378TrpHis: 0.378 ± 0.017
0.67TrpIle: 0.67 ± 0.024
0.402TrpLys: 0.402 ± 0.02
1.894TrpLeu: 1.894 ± 0.046
0.442TrpMet: 0.442 ± 0.018
0.408TrpAsn: 0.408 ± 0.02
0.809TrpPro: 0.809 ± 0.027
0.721TrpGln: 0.721 ± 0.026
1.277TrpArg: 1.277 ± 0.038
0.832TrpSer: 0.832 ± 0.028
0.81TrpThr: 0.81 ± 0.028
0.968TrpVal: 0.968 ± 0.03
0.252TrpTrp: 0.252 ± 0.016
0.253TrpTyr: 0.253 ± 0.013
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.473TyrAla: 2.473 ± 0.053
0.213TyrCys: 0.213 ± 0.011
1.457TyrAsp: 1.457 ± 0.039
1.043TyrGlu: 1.043 ± 0.034
0.741TyrPhe: 0.741 ± 0.024
2.008TyrGly: 2.008 ± 0.042
0.457TyrHis: 0.457 ± 0.019
0.798TyrIle: 0.798 ± 0.025
0.453TyrLys: 0.453 ± 0.019
2.005TyrLeu: 2.005 ± 0.039
0.422TyrMet: 0.422 ± 0.018
0.468TyrAsn: 0.468 ± 0.02
0.982TyrPro: 0.982 ± 0.028
0.589TyrGln: 0.589 ± 0.022
1.518TyrArg: 1.518 ± 0.033
0.999TyrSer: 0.999 ± 0.03
1.042TyrThr: 1.042 ± 0.031
1.432TyrVal: 1.432 ± 0.033
0.35TyrTrp: 0.35 ± 0.019
0.517TyrTyr: 0.517 ± 0.027
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4016 proteins (1263230 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski