bacterium HR34

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 7.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 573 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H6A8G8|A0A2H6A8G8_9BACT Na(+)/H(+) antiporter NhaD OS=bacterium HR34 OX=2035429 GN=nhaD PE=4 SV=1
MM1 pKa = 7.35NKK3 pKa = 9.4ILSLLLAVAISSYY16 pKa = 10.81PVFLFVEE23 pKa = 4.38PEE25 pKa = 3.76ITDD28 pKa = 3.72SVSATDD34 pKa = 3.55SVGVSLQVTEE44 pKa = 5.5EE45 pKa = 4.1IAISSPPDD53 pKa = 3.28VNLPSIGGMGTASSTGSFSWTVTTNNSAGYY83 pKa = 7.62TLAVKK88 pKa = 10.57ASTSPALQSGSDD100 pKa = 3.89SIADD104 pKa = 3.77YY105 pKa = 10.45TPATAGTPDD114 pKa = 3.36FNFSVGANDD123 pKa = 4.13AEE125 pKa = 4.28FGYY128 pKa = 10.42SVEE131 pKa = 4.58GSDD134 pKa = 3.89TAQLFLDD141 pKa = 4.52NGSACNTGTTNAVDD155 pKa = 3.8KK156 pKa = 10.69CWLNFTTTDD165 pKa = 2.82ITVARR170 pKa = 11.84STSEE174 pKa = 4.21TPSSGTPTTIKK185 pKa = 10.62LRR187 pKa = 11.84VDD189 pKa = 3.76LGTNAFVPSGTYY201 pKa = 9.4SATIIGTATTLL212 pKa = 3.37

Molecular weight: 21.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H6A857|A0A2H6A857_9BACT Uncharacterized protein OS=bacterium HR34 OX=2035429 GN=HRbin34_00451 PE=4 SV=1
MM1 pKa = 7.49LPGAKK6 pKa = 8.67TRR8 pKa = 11.84NKK10 pKa = 9.54KK11 pKa = 9.91RR12 pKa = 11.84KK13 pKa = 7.41RR14 pKa = 11.84THH16 pKa = 6.08GFLARR21 pKa = 11.84KK22 pKa = 8.82SKK24 pKa = 10.77KK25 pKa = 9.99GGKK28 pKa = 9.19KK29 pKa = 9.31VIKK32 pKa = 10.18RR33 pKa = 11.84RR34 pKa = 11.84IKK36 pKa = 10.2KK37 pKa = 8.04GRR39 pKa = 11.84KK40 pKa = 9.13RR41 pKa = 11.84ITVV44 pKa = 3.25

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

573

0

573

166225

31

1192

290.1

33.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.905 ± 0.069

0.886 ± 0.038

4.883 ± 0.063

7.98 ± 0.122

5.655 ± 0.12

5.867 ± 0.079

1.213 ± 0.036

9.805 ± 0.088

11.301 ± 0.136

9.572 ± 0.095

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.704 ± 0.035

5.401 ± 0.092

3.4 ± 0.056

2.908 ± 0.063

3.657 ± 0.077

5.748 ± 0.096

4.082 ± 0.068

6.472 ± 0.089

0.794 ± 0.038

3.768 ± 0.066
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski