Amino acid dipepetide frequency for bacterium HR34

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.436AlaAla: 2.436 ± 0.162
0.529AlaCys: 0.529 ± 0.059
2.13AlaAsp: 2.13 ± 0.1
2.815AlaGlu: 2.815 ± 0.138
2.954AlaPhe: 2.954 ± 0.163
3.261AlaGly: 3.261 ± 0.162
0.698AlaHis: 0.698 ± 0.07
4.837AlaIle: 4.837 ± 0.189
5.571AlaLys: 5.571 ± 0.188
5.132AlaLeu: 5.132 ± 0.193
0.914AlaMet: 0.914 ± 0.07
1.979AlaAsn: 1.979 ± 0.11
1.378AlaPro: 1.378 ± 0.089
1.907AlaGln: 1.907 ± 0.115
2.316AlaArg: 2.316 ± 0.124
2.725AlaSer: 2.725 ± 0.134
2.244AlaThr: 2.244 ± 0.112
3.441AlaVal: 3.441 ± 0.144
0.289AlaTrp: 0.289 ± 0.043
1.498AlaTyr: 1.498 ± 0.106
0.0AlaXaa: 0.0 ± 0.0
Cys
0.499CysAla: 0.499 ± 0.063
0.114CysCys: 0.114 ± 0.028
0.578CysAsp: 0.578 ± 0.067
0.632CysGlu: 0.632 ± 0.063
0.535CysPhe: 0.535 ± 0.058
0.77CysGly: 0.77 ± 0.082
0.229CysHis: 0.229 ± 0.036
0.511CysIle: 0.511 ± 0.055
0.963CysLys: 0.963 ± 0.075
0.698CysLeu: 0.698 ± 0.066
0.102CysMet: 0.102 ± 0.024
0.427CysAsn: 0.427 ± 0.052
0.535CysPro: 0.535 ± 0.062
0.253CysGln: 0.253 ± 0.044
0.307CysArg: 0.307 ± 0.046
0.608CysSer: 0.608 ± 0.067
0.253CysThr: 0.253 ± 0.039
0.499CysVal: 0.499 ± 0.059
0.078CysTrp: 0.078 ± 0.024
0.265CysTyr: 0.265 ± 0.044
0.0CysXaa: 0.0 ± 0.0
Asp
2.021AspAla: 2.021 ± 0.108
0.421AspCys: 0.421 ± 0.052
2.154AspAsp: 2.154 ± 0.125
3.965AspGlu: 3.965 ± 0.17
3.549AspPhe: 3.549 ± 0.137
2.424AspGly: 2.424 ± 0.134
0.499AspHis: 0.499 ± 0.06
6.052AspIle: 6.052 ± 0.18
5.264AspLys: 5.264 ± 0.197
4.837AspLeu: 4.837 ± 0.17
0.704AspMet: 0.704 ± 0.061
2.629AspAsn: 2.629 ± 0.134
1.727AspPro: 1.727 ± 0.112
0.77AspGln: 0.77 ± 0.068
1.582AspArg: 1.582 ± 0.095
2.22AspSer: 2.22 ± 0.12
1.805AspThr: 1.805 ± 0.096
3.489AspVal: 3.489 ± 0.15
0.421AspTrp: 0.421 ± 0.054
2.292AspTyr: 2.292 ± 0.139
0.0AspXaa: 0.0 ± 0.0
Glu
3.658GluAla: 3.658 ± 0.175
0.584GluCys: 0.584 ± 0.063
4.085GluAsp: 4.085 ± 0.149
6.293GluGlu: 6.293 ± 0.248
3.952GluPhe: 3.952 ± 0.161
4.554GluGly: 4.554 ± 0.184
0.758GluHis: 0.758 ± 0.069
7.851GluIle: 7.851 ± 0.214
11.936GluLys: 11.936 ± 0.315
6.63GluLeu: 6.63 ± 0.237
1.42GluMet: 1.42 ± 0.086
6.022GluAsn: 6.022 ± 0.225
1.576GluPro: 1.576 ± 0.086
2.136GluGln: 2.136 ± 0.134
3.447GluArg: 3.447 ± 0.135
3.399GluSer: 3.399 ± 0.168
2.725GluThr: 2.725 ± 0.131
5.523GluVal: 5.523 ± 0.222
0.68GluTrp: 0.68 ± 0.072
2.575GluTyr: 2.575 ± 0.137
0.0GluXaa: 0.0 ± 0.0
Phe
2.99PheAla: 2.99 ± 0.132
0.602PheCys: 0.602 ± 0.056
2.761PheAsp: 2.761 ± 0.144
3.73PheGlu: 3.73 ± 0.141
3.303PhePhe: 3.303 ± 0.204
3.399PheGly: 3.399 ± 0.159
0.547PheHis: 0.547 ± 0.056
5.017PheIle: 5.017 ± 0.218
4.795PheLys: 4.795 ± 0.19
6.876PheLeu: 6.876 ± 0.315
0.848PheMet: 0.848 ± 0.079
2.912PheAsn: 2.912 ± 0.154
1.733PhePro: 1.733 ± 0.105
1.36PheGln: 1.36 ± 0.096
1.456PheArg: 1.456 ± 0.093
4.614PheSer: 4.614 ± 0.181
2.382PheThr: 2.382 ± 0.137
3.946PheVal: 3.946 ± 0.203
0.541PheTrp: 0.541 ± 0.065
2.731PheTyr: 2.731 ± 0.142
0.0PheXaa: 0.0 ± 0.0
Gly
3.068GlyAla: 3.068 ± 0.172
0.511GlyCys: 0.511 ± 0.057
3.14GlyAsp: 3.14 ± 0.15
4.452GlyGlu: 4.452 ± 0.182
3.592GlyPhe: 3.592 ± 0.138
4.211GlyGly: 4.211 ± 0.202
0.848GlyHis: 0.848 ± 0.073
5.601GlyIle: 5.601 ± 0.197
6.202GlyLys: 6.202 ± 0.203
4.933GlyLeu: 4.933 ± 0.208
1.017GlyMet: 1.017 ± 0.078
2.539GlyAsn: 2.539 ± 0.128
1.462GlyPro: 1.462 ± 0.104
1.751GlyGln: 1.751 ± 0.091
2.37GlyArg: 2.37 ± 0.15
3.092GlySer: 3.092 ± 0.142
2.364GlyThr: 2.364 ± 0.13
4.68GlyVal: 4.68 ± 0.184
0.481GlyTrp: 0.481 ± 0.054
2.352GlyTyr: 2.352 ± 0.134
0.0GlyXaa: 0.0 ± 0.0
His
0.559HisAla: 0.559 ± 0.057
0.138HisCys: 0.138 ± 0.031
0.463HisAsp: 0.463 ± 0.054
0.716HisGlu: 0.716 ± 0.07
0.602HisPhe: 0.602 ± 0.06
0.794HisGly: 0.794 ± 0.07
0.271HisHis: 0.271 ± 0.04
1.281HisIle: 1.281 ± 0.095
1.227HisLys: 1.227 ± 0.095
1.245HisLeu: 1.245 ± 0.08
0.253HisMet: 0.253 ± 0.043
0.614HisAsn: 0.614 ± 0.061
0.752HisPro: 0.752 ± 0.081
0.421HisGln: 0.421 ± 0.058
0.535HisArg: 0.535 ± 0.059
0.566HisSer: 0.566 ± 0.056
0.614HisThr: 0.614 ± 0.058
0.421HisVal: 0.421 ± 0.049
0.15HisTrp: 0.15 ± 0.03
0.511HisTyr: 0.511 ± 0.054
0.0HisXaa: 0.0 ± 0.0
Ile
4.813IleAla: 4.813 ± 0.194
0.686IleCys: 0.686 ± 0.074
4.831IleAsp: 4.831 ± 0.166
8.031IleGlu: 8.031 ± 0.243
5.673IlePhe: 5.673 ± 0.23
5.541IleGly: 5.541 ± 0.2
0.957IleHis: 0.957 ± 0.065
9.878IleIle: 9.878 ± 0.257
11.894IleLys: 11.894 ± 0.296
9.758IleLeu: 9.758 ± 0.29
1.588IleMet: 1.588 ± 0.107
4.662IleAsn: 4.662 ± 0.163
3.423IlePro: 3.423 ± 0.142
2.322IleGln: 2.322 ± 0.139
3.086IleArg: 3.086 ± 0.146
6.269IleSer: 6.269 ± 0.211
4.422IleThr: 4.422 ± 0.173
6.202IleVal: 6.202 ± 0.217
0.608IleTrp: 0.608 ± 0.068
3.411IleTyr: 3.411 ± 0.137
0.0IleXaa: 0.0 ± 0.0
Lys
5.053LysAla: 5.053 ± 0.206
0.884LysCys: 0.884 ± 0.093
6.527LysAsp: 6.527 ± 0.212
12.748LysGlu: 12.748 ± 0.33
4.422LysPhe: 4.422 ± 0.179
5.95LysGly: 5.95 ± 0.229
0.945LysHis: 0.945 ± 0.078
12.736LysIle: 12.736 ± 0.285
12.856LysLys: 12.856 ± 0.342
8.483LysLeu: 8.483 ± 0.262
2.142LysMet: 2.142 ± 0.12
7.7LysAsn: 7.7 ± 0.246
2.912LysPro: 2.912 ± 0.108
2.659LysGln: 2.659 ± 0.144
4.025LysArg: 4.025 ± 0.167
5.174LysSer: 5.174 ± 0.174
5.162LysThr: 5.162 ± 0.191
7.719LysVal: 7.719 ± 0.277
0.722LysTrp: 0.722 ± 0.069
4.193LysTyr: 4.193 ± 0.171
0.0LysXaa: 0.0 ± 0.0
Leu
5.396LeuAla: 5.396 ± 0.209
0.722LeuCys: 0.722 ± 0.064
4.356LeuAsp: 4.356 ± 0.184
7.785LeuGlu: 7.785 ± 0.221
4.819LeuPhe: 4.819 ± 0.228
5.444LeuGly: 5.444 ± 0.21
1.317LeuHis: 1.317 ± 0.091
8.663LeuIle: 8.663 ± 0.254
11.112LeuLys: 11.112 ± 0.281
8.591LeuLeu: 8.591 ± 0.286
1.751LeuMet: 1.751 ± 0.106
4.536LeuAsn: 4.536 ± 0.174
4.247LeuPro: 4.247 ± 0.154
2.954LeuGln: 2.954 ± 0.143
4.049LeuArg: 4.049 ± 0.166
6.924LeuSer: 6.924 ± 0.241
3.82LeuThr: 3.82 ± 0.157
5.204LeuVal: 5.204 ± 0.195
0.902LeuTrp: 0.902 ± 0.098
3.128LeuTyr: 3.128 ± 0.15
0.0LeuXaa: 0.0 ± 0.0
Met
1.197MetAla: 1.197 ± 0.089
0.114MetCys: 0.114 ± 0.024
0.68MetAsp: 0.68 ± 0.067
1.534MetGlu: 1.534 ± 0.098
0.926MetPhe: 0.926 ± 0.069
0.987MetGly: 0.987 ± 0.087
0.217MetHis: 0.217 ± 0.039
1.33MetIle: 1.33 ± 0.095
1.907MetLys: 1.907 ± 0.109
1.492MetLeu: 1.492 ± 0.105
0.361MetMet: 0.361 ± 0.05
0.686MetAsn: 0.686 ± 0.079
0.92MetPro: 0.92 ± 0.076
0.638MetGln: 0.638 ± 0.057
0.686MetArg: 0.686 ± 0.068
1.047MetSer: 1.047 ± 0.077
0.65MetThr: 0.65 ± 0.055
1.071MetVal: 1.071 ± 0.085
0.132MetTrp: 0.132 ± 0.027
0.469MetTyr: 0.469 ± 0.055
0.0MetXaa: 0.0 ± 0.0
Asn
1.823AsnAla: 1.823 ± 0.118
0.487AsnCys: 0.487 ± 0.047
1.931AsnAsp: 1.931 ± 0.114
3.176AsnGlu: 3.176 ± 0.156
3.561AsnPhe: 3.561 ± 0.165
2.184AsnGly: 2.184 ± 0.119
0.541AsnHis: 0.541 ± 0.05
6.16AsnIle: 6.16 ± 0.193
6.587AsnLys: 6.587 ± 0.238
5.98AsnLeu: 5.98 ± 0.19
0.896AsnMet: 0.896 ± 0.076
3.862AsnAsn: 3.862 ± 0.208
2.25AsnPro: 2.25 ± 0.128
1.654AsnGln: 1.654 ± 0.137
1.552AsnArg: 1.552 ± 0.094
2.882AsnSer: 2.882 ± 0.16
2.166AsnThr: 2.166 ± 0.127
3.225AsnVal: 3.225 ± 0.156
0.529AsnTrp: 0.529 ± 0.068
2.557AsnTyr: 2.557 ± 0.142
0.0AsnXaa: 0.0 ± 0.0
Pro
1.594ProAla: 1.594 ± 0.094
0.295ProCys: 0.295 ± 0.043
1.787ProAsp: 1.787 ± 0.11
3.291ProGlu: 3.291 ± 0.148
2.262ProPhe: 2.262 ± 0.11
1.829ProGly: 1.829 ± 0.14
0.59ProHis: 0.59 ± 0.062
2.599ProIle: 2.599 ± 0.114
3.315ProLys: 3.315 ± 0.152
3.146ProLeu: 3.146 ± 0.149
0.487ProMet: 0.487 ± 0.054
1.54ProAsn: 1.54 ± 0.093
1.275ProPro: 1.275 ± 0.096
1.354ProGln: 1.354 ± 0.088
1.077ProArg: 1.077 ± 0.091
2.1ProSer: 2.1 ± 0.105
1.57ProThr: 1.57 ± 0.104
2.166ProVal: 2.166 ± 0.12
0.319ProTrp: 0.319 ± 0.041
1.402ProTyr: 1.402 ± 0.091
0.0ProXaa: 0.0 ± 0.0
Gln
1.486GlnAla: 1.486 ± 0.103
0.211GlnCys: 0.211 ± 0.039
1.552GlnAsp: 1.552 ± 0.107
3.152GlnGlu: 3.152 ± 0.153
0.987GlnPhe: 0.987 ± 0.071
1.534GlnGly: 1.534 ± 0.111
0.211GlnHis: 0.211 ± 0.039
2.737GlnIle: 2.737 ± 0.147
3.91GlnLys: 3.91 ± 0.187
2.16GlnLeu: 2.16 ± 0.128
0.59GlnMet: 0.59 ± 0.057
1.961GlnAsn: 1.961 ± 0.134
0.908GlnPro: 0.908 ± 0.081
1.029GlnGln: 1.029 ± 0.091
1.336GlnArg: 1.336 ± 0.099
1.281GlnSer: 1.281 ± 0.099
1.408GlnThr: 1.408 ± 0.099
1.624GlnVal: 1.624 ± 0.095
0.18GlnTrp: 0.18 ± 0.032
0.824GlnTyr: 0.824 ± 0.067
0.0GlnXaa: 0.0 ± 0.0
Arg
1.793ArgAla: 1.793 ± 0.108
0.289ArgCys: 0.289 ± 0.042
1.793ArgAsp: 1.793 ± 0.105
3.393ArgGlu: 3.393 ± 0.174
1.865ArgPhe: 1.865 ± 0.114
2.232ArgGly: 2.232 ± 0.123
0.463ArgHis: 0.463 ± 0.06
3.736ArgIle: 3.736 ± 0.161
4.121ArgLys: 4.121 ± 0.182
3.11ArgLeu: 3.11 ± 0.144
0.746ArgMet: 0.746 ± 0.067
1.799ArgAsn: 1.799 ± 0.119
1.059ArgPro: 1.059 ± 0.074
1.107ArgGln: 1.107 ± 0.089
1.582ArgArg: 1.582 ± 0.116
1.648ArgSer: 1.648 ± 0.093
1.48ArgThr: 1.48 ± 0.09
2.515ArgVal: 2.515 ± 0.123
0.319ArgTrp: 0.319 ± 0.053
1.522ArgTyr: 1.522 ± 0.113
0.0ArgXaa: 0.0 ± 0.0
Ser
3.032SerAla: 3.032 ± 0.148
0.559SerCys: 0.559 ± 0.06
2.731SerAsp: 2.731 ± 0.133
3.94SerGlu: 3.94 ± 0.166
3.989SerPhe: 3.989 ± 0.189
3.561SerGly: 3.561 ± 0.159
0.884SerHis: 0.884 ± 0.078
4.939SerIle: 4.939 ± 0.202
6.124SerLys: 6.124 ± 0.197
6.365SerLeu: 6.365 ± 0.227
0.848SerMet: 0.848 ± 0.083
2.743SerAsn: 2.743 ± 0.148
1.967SerPro: 1.967 ± 0.107
2.13SerGln: 2.13 ± 0.116
1.865SerArg: 1.865 ± 0.111
3.604SerSer: 3.604 ± 0.163
2.063SerThr: 2.063 ± 0.108
3.598SerVal: 3.598 ± 0.139
0.385SerTrp: 0.385 ± 0.044
2.148SerTyr: 2.148 ± 0.127
0.0SerXaa: 0.0 ± 0.0
Thr
2.256ThrAla: 2.256 ± 0.117
0.343ThrCys: 0.343 ± 0.047
1.877ThrAsp: 1.877 ± 0.102
2.268ThrGlu: 2.268 ± 0.121
2.136ThrPhe: 2.136 ± 0.099
3.249ThrGly: 3.249 ± 0.139
0.517ThrHis: 0.517 ± 0.058
4.151ThrIle: 4.151 ± 0.14
4.115ThrLys: 4.115 ± 0.159
4.139ThrLeu: 4.139 ± 0.141
0.698ThrMet: 0.698 ± 0.065
1.787ThrAsn: 1.787 ± 0.113
1.931ThrPro: 1.931 ± 0.118
1.576ThrGln: 1.576 ± 0.11
1.426ThrArg: 1.426 ± 0.098
2.43ThrSer: 2.43 ± 0.132
2.268ThrThr: 2.268 ± 0.141
2.039ThrVal: 2.039 ± 0.098
0.211ThrTrp: 0.211 ± 0.032
1.402ThrTyr: 1.402 ± 0.097
0.0ThrXaa: 0.0 ± 0.0
Val
3.58ValAla: 3.58 ± 0.157
0.782ValCys: 0.782 ± 0.073
3.369ValAsp: 3.369 ± 0.139
4.59ValGlu: 4.59 ± 0.177
4.187ValPhe: 4.187 ± 0.202
4.067ValGly: 4.067 ± 0.173
0.854ValHis: 0.854 ± 0.081
5.673ValIle: 5.673 ± 0.195
6.702ValLys: 6.702 ± 0.21
7.207ValLeu: 7.207 ± 0.208
0.999ValMet: 0.999 ± 0.093
2.918ValAsn: 2.918 ± 0.15
2.208ValPro: 2.208 ± 0.121
1.654ValGln: 1.654 ± 0.096
2.268ValArg: 2.268 ± 0.105
4.103ValSer: 4.103 ± 0.149
1.751ValThr: 1.751 ± 0.117
4.963ValVal: 4.963 ± 0.219
0.535ValTrp: 0.535 ± 0.056
2.31ValTyr: 2.31 ± 0.121
0.0ValXaa: 0.0 ± 0.0
Trp
0.343TrpAla: 0.343 ± 0.052
0.09TrpCys: 0.09 ± 0.021
0.301TrpAsp: 0.301 ± 0.049
0.71TrpGlu: 0.71 ± 0.073
0.445TrpPhe: 0.445 ± 0.052
0.553TrpGly: 0.553 ± 0.061
0.168TrpHis: 0.168 ± 0.034
0.698TrpIle: 0.698 ± 0.07
0.572TrpLys: 0.572 ± 0.065
0.836TrpLeu: 0.836 ± 0.079
0.174TrpMet: 0.174 ± 0.03
0.403TrpAsn: 0.403 ± 0.056
0.271TrpPro: 0.271 ± 0.045
0.463TrpGln: 0.463 ± 0.059
0.325TrpArg: 0.325 ± 0.046
0.421TrpSer: 0.421 ± 0.054
0.277TrpThr: 0.277 ± 0.043
0.439TrpVal: 0.439 ± 0.052
0.144TrpTrp: 0.144 ± 0.035
0.307TrpTyr: 0.307 ± 0.043
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.456TyrAla: 1.456 ± 0.096
0.493TyrCys: 0.493 ± 0.058
1.781TyrAsp: 1.781 ± 0.1
2.581TyrGlu: 2.581 ± 0.138
2.791TyrPhe: 2.791 ± 0.156
2.124TyrGly: 2.124 ± 0.126
0.62TyrHis: 0.62 ± 0.07
3.604TyrIle: 3.604 ± 0.136
3.838TyrLys: 3.838 ± 0.147
4.103TyrLeu: 4.103 ± 0.179
0.499TyrMet: 0.499 ± 0.057
2.286TyrAsn: 2.286 ± 0.13
1.462TyrPro: 1.462 ± 0.1
0.945TyrGln: 0.945 ± 0.079
1.281TyrArg: 1.281 ± 0.092
2.37TyrSer: 2.37 ± 0.127
1.396TyrThr: 1.396 ± 0.084
1.955TyrVal: 1.955 ± 0.113
0.313TyrTrp: 0.313 ± 0.049
1.781TyrTyr: 1.781 ± 0.109
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 573 proteins (166225 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski