Amino acid dipepetide frequency for Bacillus lehensis G1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.747AlaAla: 5.747 ± 0.097
0.729AlaCys: 0.729 ± 0.029
3.64AlaAsp: 3.64 ± 0.17
4.598AlaGlu: 4.598 ± 0.07
3.815AlaPhe: 3.815 ± 0.058
5.289AlaGly: 5.289 ± 0.091
1.65AlaHis: 1.65 ± 0.042
6.174AlaIle: 6.174 ± 0.09
4.243AlaLys: 4.243 ± 0.067
8.184AlaLeu: 8.184 ± 0.106
2.117AlaMet: 2.117 ± 0.043
2.918AlaAsn: 2.918 ± 0.047
2.208AlaPro: 2.208 ± 0.05
2.575AlaGln: 2.575 ± 0.049
2.868AlaArg: 2.868 ± 0.058
4.709AlaSer: 4.709 ± 0.077
4.622AlaThr: 4.622 ± 0.179
5.501AlaVal: 5.501 ± 0.08
0.638AlaTrp: 0.638 ± 0.028
2.693AlaTyr: 2.693 ± 0.047
0.0AlaXaa: 0.0 ± 0.0
Cys
0.419CysAla: 0.419 ± 0.02
0.086CysCys: 0.086 ± 0.01
0.375CysAsp: 0.375 ± 0.02
0.431CysGlu: 0.431 ± 0.02
0.33CysPhe: 0.33 ± 0.018
0.655CysGly: 0.655 ± 0.025
0.209CysHis: 0.209 ± 0.014
0.466CysIle: 0.466 ± 0.019
0.303CysLys: 0.303 ± 0.016
0.743CysLeu: 0.743 ± 0.026
0.186CysMet: 0.186 ± 0.013
0.213CysAsn: 0.213 ± 0.016
0.313CysPro: 0.313 ± 0.019
0.264CysGln: 0.264 ± 0.017
0.267CysArg: 0.267 ± 0.016
0.469CysSer: 0.469 ± 0.02
0.4CysThr: 0.4 ± 0.021
0.459CysVal: 0.459 ± 0.019
0.068CysTrp: 0.068 ± 0.007
0.227CysTyr: 0.227 ± 0.014
0.0CysXaa: 0.0 ± 0.0
Asp
3.843AspAla: 3.843 ± 0.167
0.339AspCys: 0.339 ± 0.018
2.72AspAsp: 2.72 ± 0.066
4.613AspGlu: 4.613 ± 0.074
2.139AspPhe: 2.139 ± 0.049
3.586AspGly: 3.586 ± 0.063
1.401AspHis: 1.401 ± 0.038
3.473AspIle: 3.473 ± 0.059
2.408AspLys: 2.408 ± 0.057
5.093AspLeu: 5.093 ± 0.068
1.277AspMet: 1.277 ± 0.036
1.566AspAsn: 1.566 ± 0.043
1.993AspPro: 1.993 ± 0.042
2.375AspGln: 2.375 ± 0.048
2.452AspArg: 2.452 ± 0.045
2.644AspSer: 2.644 ± 0.062
2.539AspThr: 2.539 ± 0.052
4.324AspVal: 4.324 ± 0.061
0.647AspTrp: 0.647 ± 0.024
2.094AspTyr: 2.094 ± 0.047
0.0AspXaa: 0.0 ± 0.0
Glu
6.262GluAla: 6.262 ± 0.085
0.387GluCys: 0.387 ± 0.016
4.035GluAsp: 4.035 ± 0.067
7.603GluGlu: 7.603 ± 0.125
2.189GluPhe: 2.189 ± 0.052
4.426GluGly: 4.426 ± 0.071
1.683GluHis: 1.683 ± 0.035
4.276GluIle: 4.276 ± 0.065
5.29GluLys: 5.29 ± 0.076
7.251GluLeu: 7.251 ± 0.088
2.185GluMet: 2.185 ± 0.04
3.218GluAsn: 3.218 ± 0.057
2.229GluPro: 2.229 ± 0.052
4.115GluGln: 4.115 ± 0.075
4.068GluArg: 4.068 ± 0.059
3.756GluSer: 3.756 ± 0.057
4.388GluThr: 4.388 ± 0.068
4.655GluVal: 4.655 ± 0.073
0.981GluTrp: 0.981 ± 0.03
1.924GluTyr: 1.924 ± 0.043
0.0GluXaa: 0.0 ± 0.0
Phe
3.22PheAla: 3.22 ± 0.057
0.32PheCys: 0.32 ± 0.017
2.314PheAsp: 2.314 ± 0.042
2.669PheGlu: 2.669 ± 0.055
2.527PhePhe: 2.527 ± 0.065
3.177PheGly: 3.177 ± 0.065
1.093PheHis: 1.093 ± 0.031
3.798PheIle: 3.798 ± 0.066
2.049PheLys: 2.049 ± 0.044
4.817PheLeu: 4.817 ± 0.087
1.165PheMet: 1.165 ± 0.036
1.726PheAsn: 1.726 ± 0.043
1.685PhePro: 1.685 ± 0.043
1.671PheGln: 1.671 ± 0.039
1.532PheArg: 1.532 ± 0.038
3.376PheSer: 3.376 ± 0.064
2.77PheThr: 2.77 ± 0.049
3.282PheVal: 3.282 ± 0.06
0.46PheTrp: 0.46 ± 0.02
1.721PheTyr: 1.721 ± 0.042
0.0PheXaa: 0.0 ± 0.0
Gly
5.356GlyAla: 5.356 ± 0.189
0.561GlyCys: 0.561 ± 0.022
3.234GlyAsp: 3.234 ± 0.055
4.62GlyGlu: 4.62 ± 0.071
3.504GlyPhe: 3.504 ± 0.058
4.758GlyGly: 4.758 ± 0.083
1.419GlyHis: 1.419 ± 0.038
5.545GlyIle: 5.545 ± 0.076
4.054GlyLys: 4.054 ± 0.064
6.769GlyLeu: 6.769 ± 0.096
2.159GlyMet: 2.159 ± 0.05
2.569GlyAsn: 2.569 ± 0.054
1.858GlyPro: 1.858 ± 0.068
2.383GlyGln: 2.383 ± 0.043
2.654GlyArg: 2.654 ± 0.045
4.205GlySer: 4.205 ± 0.071
4.166GlyThr: 4.166 ± 0.062
5.5GlyVal: 5.5 ± 0.217
0.81GlyTrp: 0.81 ± 0.027
2.74GlyTyr: 2.74 ± 0.047
0.0GlyXaa: 0.0 ± 0.0
His
1.695HisAla: 1.695 ± 0.041
0.21HisCys: 0.21 ± 0.014
1.209HisAsp: 1.209 ± 0.033
1.607HisGlu: 1.607 ± 0.041
1.174HisPhe: 1.174 ± 0.034
1.454HisGly: 1.454 ± 0.038
0.842HisHis: 0.842 ± 0.028
1.582HisIle: 1.582 ± 0.04
0.998HisLys: 0.998 ± 0.029
2.419HisLeu: 2.419 ± 0.054
0.573HisMet: 0.573 ± 0.023
0.733HisAsn: 0.733 ± 0.029
1.138HisPro: 1.138 ± 0.034
1.062HisGln: 1.062 ± 0.03
0.93HisArg: 0.93 ± 0.031
1.497HisSer: 1.497 ± 0.038
1.322HisThr: 1.322 ± 0.033
1.828HisVal: 1.828 ± 0.045
0.262HisTrp: 0.262 ± 0.014
1.148HisTyr: 1.148 ± 0.029
0.0HisXaa: 0.0 ± 0.0
Ile
5.87IleAla: 5.87 ± 0.082
0.561IleCys: 0.561 ± 0.024
4.237IleAsp: 4.237 ± 0.064
5.487IleGlu: 5.487 ± 0.075
2.629IlePhe: 2.629 ± 0.059
5.991IleGly: 5.991 ± 0.087
1.764IleHis: 1.764 ± 0.043
4.759IleIle: 4.759 ± 0.071
3.502IleLys: 3.502 ± 0.053
6.239IleLeu: 6.239 ± 0.091
1.606IleMet: 1.606 ± 0.037
2.6IleAsn: 2.6 ± 0.064
3.176IlePro: 3.176 ± 0.057
3.024IleGln: 3.024 ± 0.055
3.111IleArg: 3.111 ± 0.051
4.268IleSer: 4.268 ± 0.065
3.877IleThr: 3.877 ± 0.072
5.604IleVal: 5.604 ± 0.081
0.653IleTrp: 0.653 ± 0.023
2.13IleTyr: 2.13 ± 0.049
0.0IleXaa: 0.0 ± 0.0
Lys
4.468LysAla: 4.468 ± 0.073
0.229LysCys: 0.229 ± 0.013
3.162LysAsp: 3.162 ± 0.054
6.122LysGlu: 6.122 ± 0.087
1.103LysPhe: 1.103 ± 0.032
3.965LysGly: 3.965 ± 0.067
1.403LysHis: 1.403 ± 0.04
2.913LysIle: 2.913 ± 0.055
4.956LysLys: 4.956 ± 0.082
4.504LysLeu: 4.504 ± 0.061
1.631LysMet: 1.631 ± 0.044
2.321LysAsn: 2.321 ± 0.048
2.134LysPro: 2.134 ± 0.043
3.635LysGln: 3.635 ± 0.064
3.432LysArg: 3.432 ± 0.054
2.776LysSer: 2.776 ± 0.057
3.364LysThr: 3.364 ± 0.06
3.62LysVal: 3.62 ± 0.059
0.716LysTrp: 0.716 ± 0.027
1.206LysTyr: 1.206 ± 0.032
0.0LysXaa: 0.0 ± 0.0
Leu
8.021LeuAla: 8.021 ± 0.09
0.681LeuCys: 0.681 ± 0.025
4.905LeuAsp: 4.905 ± 0.073
6.497LeuGlu: 6.497 ± 0.089
5.469LeuPhe: 5.469 ± 0.094
6.165LeuGly: 6.165 ± 0.095
2.327LeuHis: 2.327 ± 0.048
6.951LeuIle: 6.951 ± 0.086
5.663LeuLys: 5.663 ± 0.08
11.158LeuLeu: 11.158 ± 0.139
2.593LeuMet: 2.593 ± 0.052
4.048LeuAsn: 4.048 ± 0.059
4.004LeuPro: 4.004 ± 0.064
3.806LeuGln: 3.806 ± 0.059
3.699LeuArg: 3.699 ± 0.057
7.069LeuSer: 7.069 ± 0.081
6.441LeuThr: 6.441 ± 0.067
6.873LeuVal: 6.873 ± 0.093
0.764LeuTrp: 0.764 ± 0.025
3.309LeuTyr: 3.309 ± 0.058
0.0LeuXaa: 0.0 ± 0.0
Met
2.085MetAla: 2.085 ± 0.042
0.128MetCys: 0.128 ± 0.01
1.51MetAsp: 1.51 ± 0.041
1.85MetGlu: 1.85 ± 0.042
1.004MetPhe: 1.004 ± 0.031
1.556MetGly: 1.556 ± 0.04
0.507MetHis: 0.507 ± 0.023
2.041MetIle: 2.041 ± 0.047
2.195MetLys: 2.195 ± 0.039
2.539MetLeu: 2.539 ± 0.053
0.895MetMet: 0.895 ± 0.03
1.629MetAsn: 1.629 ± 0.038
0.986MetPro: 0.986 ± 0.026
0.92MetGln: 0.92 ± 0.033
1.175MetArg: 1.175 ± 0.032
1.757MetSer: 1.757 ± 0.036
1.776MetThr: 1.776 ± 0.04
1.758MetVal: 1.758 ± 0.044
0.212MetTrp: 0.212 ± 0.014
0.761MetTyr: 0.761 ± 0.026
0.0MetXaa: 0.0 ± 0.0
Asn
2.583AsnAla: 2.583 ± 0.045
0.239AsnCys: 0.239 ± 0.013
2.287AsnAsp: 2.287 ± 0.047
3.697AsnGlu: 3.697 ± 0.063
1.214AsnPhe: 1.214 ± 0.03
3.285AsnGly: 3.285 ± 0.063
1.148AsnHis: 1.148 ± 0.031
2.292AsnIle: 2.292 ± 0.048
2.205AsnLys: 2.205 ± 0.044
3.133AsnLeu: 3.133 ± 0.059
1.032AsnMet: 1.032 ± 0.035
1.498AsnAsn: 1.498 ± 0.042
1.853AsnPro: 1.853 ± 0.042
2.214AsnGln: 2.214 ± 0.049
2.254AsnArg: 2.254 ± 0.046
1.762AsnSer: 1.762 ± 0.042
1.932AsnThr: 1.932 ± 0.039
3.09AsnVal: 3.09 ± 0.054
0.537AsnTrp: 0.537 ± 0.026
1.288AsnTyr: 1.288 ± 0.034
0.0AsnXaa: 0.0 ± 0.0
Pro
2.415ProAla: 2.415 ± 0.051
0.177ProCys: 0.177 ± 0.014
1.901ProAsp: 1.901 ± 0.044
2.604ProGlu: 2.604 ± 0.058
2.166ProPhe: 2.166 ± 0.045
2.231ProGly: 2.231 ± 0.053
0.887ProHis: 0.887 ± 0.029
2.896ProIle: 2.896 ± 0.053
1.8ProLys: 1.8 ± 0.041
3.915ProLeu: 3.915 ± 0.061
0.866ProMet: 0.866 ± 0.028
1.582ProAsn: 1.582 ± 0.036
1.017ProPro: 1.017 ± 0.031
1.147ProGln: 1.147 ± 0.032
1.08ProArg: 1.08 ± 0.035
2.447ProSer: 2.447 ± 0.048
2.332ProThr: 2.332 ± 0.064
2.794ProVal: 2.794 ± 0.074
0.391ProTrp: 0.391 ± 0.018
1.431ProTyr: 1.431 ± 0.039
0.0ProXaa: 0.0 ± 0.0
Gln
3.505GlnAla: 3.505 ± 0.055
0.206GlnCys: 0.206 ± 0.014
1.778GlnAsp: 1.778 ± 0.04
3.101GlnGlu: 3.101 ± 0.054
1.809GlnPhe: 1.809 ± 0.044
2.341GlnGly: 2.341 ± 0.053
1.011GlnHis: 1.011 ± 0.036
2.338GlnIle: 2.338 ± 0.043
2.495GlnLys: 2.495 ± 0.049
5.042GlnLeu: 5.042 ± 0.084
1.169GlnMet: 1.169 ± 0.028
1.425GlnAsn: 1.425 ± 0.035
1.388GlnPro: 1.388 ± 0.041
2.183GlnGln: 2.183 ± 0.047
1.796GlnArg: 1.796 ± 0.044
2.721GlnSer: 2.721 ± 0.053
2.596GlnThr: 2.596 ± 0.044
2.676GlnVal: 2.676 ± 0.051
0.512GlnTrp: 0.512 ± 0.023
1.289GlnTyr: 1.289 ± 0.031
0.0GlnXaa: 0.0 ± 0.0
Arg
2.748ArgAla: 2.748 ± 0.057
0.271ArgCys: 0.271 ± 0.016
2.112ArgAsp: 2.112 ± 0.048
3.149ArgGlu: 3.149 ± 0.056
2.154ArgPhe: 2.154 ± 0.046
2.488ArgGly: 2.488 ± 0.045
0.897ArgHis: 0.897 ± 0.028
3.048ArgIle: 3.048 ± 0.054
2.887ArgLys: 2.887 ± 0.053
4.491ArgLeu: 4.491 ± 0.067
1.365ArgMet: 1.365 ± 0.032
1.736ArgAsn: 1.736 ± 0.038
1.362ArgPro: 1.362 ± 0.037
1.712ArgGln: 1.712 ± 0.038
1.863ArgArg: 1.863 ± 0.039
2.608ArgSer: 2.608 ± 0.056
2.445ArgThr: 2.445 ± 0.047
2.935ArgVal: 2.935 ± 0.05
0.468ArgTrp: 0.468 ± 0.021
1.762ArgTyr: 1.762 ± 0.038
0.0ArgXaa: 0.0 ± 0.0
Ser
3.85SerAla: 3.85 ± 0.063
0.389SerCys: 0.389 ± 0.019
2.89SerAsp: 2.89 ± 0.064
3.915SerGlu: 3.915 ± 0.072
3.675SerPhe: 3.675 ± 0.056
4.369SerGly: 4.369 ± 0.071
1.411SerHis: 1.411 ± 0.035
5.268SerIle: 5.268 ± 0.073
3.195SerLys: 3.195 ± 0.057
6.589SerLeu: 6.589 ± 0.082
1.79SerMet: 1.79 ± 0.033
2.299SerAsn: 2.299 ± 0.048
2.094SerPro: 2.094 ± 0.044
2.065SerGln: 2.065 ± 0.041
2.314SerArg: 2.314 ± 0.045
4.068SerSer: 4.068 ± 0.064
3.57SerThr: 3.57 ± 0.06
4.215SerVal: 4.215 ± 0.057
0.674SerTrp: 0.674 ± 0.024
2.504SerTyr: 2.504 ± 0.047
0.0SerXaa: 0.0 ± 0.0
Thr
4.31ThrAla: 4.31 ± 0.066
0.398ThrCys: 0.398 ± 0.018
2.909ThrAsp: 2.909 ± 0.05
3.756ThrGlu: 3.756 ± 0.062
2.939ThrPhe: 2.939 ± 0.049
5.063ThrGly: 5.063 ± 0.465
1.246ThrHis: 1.246 ± 0.036
5.174ThrIle: 5.174 ± 0.067
3.113ThrLys: 3.113 ± 0.05
5.979ThrLeu: 5.979 ± 0.068
1.596ThrMet: 1.596 ± 0.041
2.638ThrAsn: 2.638 ± 0.047
2.28ThrPro: 2.28 ± 0.048
1.611ThrGln: 1.611 ± 0.034
1.955ThrArg: 1.955 ± 0.049
3.587ThrSer: 3.587 ± 0.054
3.498ThrThr: 3.498 ± 0.056
4.577ThrVal: 4.577 ± 0.069
0.57ThrTrp: 0.57 ± 0.021
2.238ThrTyr: 2.238 ± 0.044
0.0ThrXaa: 0.0 ± 0.0
Val
5.515ValAla: 5.515 ± 0.086
0.619ValCys: 0.619 ± 0.022
3.838ValAsp: 3.838 ± 0.063
5.18ValGlu: 5.18 ± 0.07
3.323ValPhe: 3.323 ± 0.062
4.939ValGly: 4.939 ± 0.068
1.616ValHis: 1.616 ± 0.037
5.207ValIle: 5.207 ± 0.08
3.918ValLys: 3.918 ± 0.068
7.045ValLeu: 7.045 ± 0.08
1.891ValMet: 1.891 ± 0.043
2.99ValAsn: 2.99 ± 0.05
2.734ValPro: 2.734 ± 0.051
2.597ValGln: 2.597 ± 0.045
2.94ValArg: 2.94 ± 0.052
4.643ValSer: 4.643 ± 0.068
4.859ValThr: 4.859 ± 0.225
5.277ValVal: 5.277 ± 0.089
0.666ValTrp: 0.666 ± 0.026
2.391ValTyr: 2.391 ± 0.049
0.0ValXaa: 0.0 ± 0.0
Trp
0.613TrpAla: 0.613 ± 0.025
0.079TrpCys: 0.079 ± 0.009
0.521TrpAsp: 0.521 ± 0.024
0.673TrpGlu: 0.673 ± 0.027
0.561TrpPhe: 0.561 ± 0.023
0.628TrpGly: 0.628 ± 0.023
0.267TrpHis: 0.267 ± 0.016
0.798TrpIle: 0.798 ± 0.027
0.626TrpLys: 0.626 ± 0.024
1.255TrpLeu: 1.255 ± 0.035
0.357TrpMet: 0.357 ± 0.018
0.548TrpAsn: 0.548 ± 0.021
0.275TrpPro: 0.275 ± 0.018
0.452TrpGln: 0.452 ± 0.02
0.446TrpArg: 0.446 ± 0.021
0.644TrpSer: 0.644 ± 0.025
0.611TrpThr: 0.611 ± 0.027
0.684TrpVal: 0.684 ± 0.022
0.136TrpTrp: 0.136 ± 0.012
0.36TrpTyr: 0.36 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.391TyrAla: 2.391 ± 0.046
0.276TyrCys: 0.276 ± 0.018
1.951TyrAsp: 1.951 ± 0.043
2.749TyrGlu: 2.749 ± 0.056
1.649TyrPhe: 1.649 ± 0.04
2.5TyrGly: 2.5 ± 0.047
0.901TyrHis: 0.901 ± 0.032
2.088TyrIle: 2.088 ± 0.042
1.759TyrLys: 1.759 ± 0.048
3.277TyrLeu: 3.277 ± 0.061
0.83TyrMet: 0.83 ± 0.025
1.286TyrAsn: 1.286 ± 0.032
1.351TyrPro: 1.351 ± 0.036
1.572TyrGln: 1.572 ± 0.038
1.665TyrArg: 1.665 ± 0.038
2.143TyrSer: 2.143 ± 0.043
1.926TyrThr: 1.926 ± 0.041
2.536TyrVal: 2.536 ± 0.049
0.366TyrTrp: 0.366 ± 0.019
1.347TyrTyr: 1.347 ± 0.038
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4016 proteins (1166809 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski