Amino acid dipepetide frequency for Sphingobium sp. YR768

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.765AlaAla: 17.765 ± 0.148
1.094AlaCys: 1.094 ± 0.027
8.065AlaAsp: 8.065 ± 0.076
6.649AlaGlu: 6.649 ± 0.074
4.365AlaPhe: 4.365 ± 0.057
10.804AlaGly: 10.804 ± 0.092
2.437AlaHis: 2.437 ± 0.041
7.139AlaIle: 7.139 ± 0.075
4.143AlaLys: 4.143 ± 0.068
13.7AlaLeu: 13.7 ± 0.124
4.015AlaMet: 4.015 ± 0.05
3.206AlaAsn: 3.206 ± 0.049
6.218AlaPro: 6.218 ± 0.065
5.094AlaGln: 5.094 ± 0.066
9.108AlaArg: 9.108 ± 0.096
6.599AlaSer: 6.599 ± 0.081
6.566AlaThr: 6.566 ± 0.08
7.973AlaVal: 7.973 ± 0.084
1.563AlaTrp: 1.563 ± 0.033
2.843AlaTyr: 2.843 ± 0.042
0.0AlaXaa: 0.0 ± 0.0
Cys
1.006CysAla: 1.006 ± 0.026
0.103CysCys: 0.103 ± 0.009
0.514CysAsp: 0.514 ± 0.018
0.374CysGlu: 0.374 ± 0.014
0.299CysPhe: 0.299 ± 0.013
0.846CysGly: 0.846 ± 0.025
0.22CysHis: 0.22 ± 0.013
0.385CysIle: 0.385 ± 0.013
0.179CysLys: 0.179 ± 0.011
0.752CysLeu: 0.752 ± 0.02
0.17CysMet: 0.17 ± 0.01
0.194CysAsn: 0.194 ± 0.012
0.454CysPro: 0.454 ± 0.018
0.219CysGln: 0.219 ± 0.011
0.542CysArg: 0.542 ± 0.021
0.428CysSer: 0.428 ± 0.013
0.401CysThr: 0.401 ± 0.016
0.537CysVal: 0.537 ± 0.019
0.134CysTrp: 0.134 ± 0.009
0.181CysTyr: 0.181 ± 0.009
0.0CysXaa: 0.0 ± 0.0
Asp
8.009AspAla: 8.009 ± 0.063
0.475AspCys: 0.475 ± 0.018
3.423AspAsp: 3.423 ± 0.054
3.055AspGlu: 3.055 ± 0.048
2.275AspPhe: 2.275 ± 0.034
6.021AspGly: 6.021 ± 0.072
1.518AspHis: 1.518 ± 0.034
3.4AspIle: 3.4 ± 0.044
1.846AspLys: 1.846 ± 0.031
5.945AspLeu: 5.945 ± 0.066
1.682AspMet: 1.682 ± 0.033
1.559AspAsn: 1.559 ± 0.03
3.767AspPro: 3.767 ± 0.055
1.999AspGln: 1.999 ± 0.034
5.089AspArg: 5.089 ± 0.065
2.639AspSer: 2.639 ± 0.042
2.611AspThr: 2.611 ± 0.051
4.05AspVal: 4.05 ± 0.052
1.225AspTrp: 1.225 ± 0.029
1.843AspTyr: 1.843 ± 0.031
0.0AspXaa: 0.0 ± 0.0
Glu
7.207GluAla: 7.207 ± 0.068
0.291GluCys: 0.291 ± 0.015
2.63GluAsp: 2.63 ± 0.042
2.856GluGlu: 2.856 ± 0.06
1.323GluPhe: 1.323 ± 0.03
4.305GluGly: 4.305 ± 0.056
0.995GluHis: 0.995 ± 0.027
2.691GluIle: 2.691 ± 0.036
2.024GluLys: 2.024 ± 0.039
4.402GluLeu: 4.402 ± 0.061
1.404GluMet: 1.404 ± 0.028
1.304GluAsn: 1.304 ± 0.031
2.258GluPro: 2.258 ± 0.039
2.111GluGln: 2.111 ± 0.038
4.42GluArg: 4.42 ± 0.061
2.195GluSer: 2.195 ± 0.04
2.984GluThr: 2.984 ± 0.045
3.127GluVal: 3.127 ± 0.046
0.727GluTrp: 0.727 ± 0.022
0.915GluTyr: 0.915 ± 0.025
0.0GluXaa: 0.0 ± 0.0
Phe
4.644PheAla: 4.644 ± 0.047
0.338PheCys: 0.338 ± 0.016
2.79PheAsp: 2.79 ± 0.042
1.84PheGlu: 1.84 ± 0.036
1.21PhePhe: 1.21 ± 0.029
3.633PheGly: 3.633 ± 0.049
0.738PheHis: 0.738 ± 0.02
1.502PheIle: 1.502 ± 0.031
0.876PheLys: 0.876 ± 0.024
3.074PheLeu: 3.074 ± 0.049
0.77PheMet: 0.77 ± 0.02
1.076PheAsn: 1.076 ± 0.028
1.511PhePro: 1.511 ± 0.03
0.952PheGln: 0.952 ± 0.026
2.243PheArg: 2.243 ± 0.035
2.181PheSer: 2.181 ± 0.036
2.044PheThr: 2.044 ± 0.038
2.35PheVal: 2.35 ± 0.039
0.495PheTrp: 0.495 ± 0.014
0.962PheTyr: 0.962 ± 0.024
0.0PheXaa: 0.0 ± 0.0
Gly
9.771GlyAla: 9.771 ± 0.089
0.81GlyCys: 0.81 ± 0.025
5.053GlyAsp: 5.053 ± 0.074
4.405GlyGlu: 4.405 ± 0.052
3.634GlyPhe: 3.634 ± 0.049
8.014GlyGly: 8.014 ± 0.108
1.93GlyHis: 1.93 ± 0.04
4.462GlyIle: 4.462 ± 0.06
3.443GlyLys: 3.443 ± 0.049
8.641GlyLeu: 8.641 ± 0.074
2.38GlyMet: 2.38 ± 0.04
2.43GlyAsn: 2.43 ± 0.063
3.636GlyPro: 3.636 ± 0.049
3.269GlyGln: 3.269 ± 0.042
6.389GlyArg: 6.389 ± 0.071
4.964GlySer: 4.964 ± 0.069
4.774GlyThr: 4.774 ± 0.06
6.23GlyVal: 6.23 ± 0.066
1.712GlyTrp: 1.712 ± 0.033
2.504GlyTyr: 2.504 ± 0.041
0.0GlyXaa: 0.0 ± 0.0
His
2.402HisAla: 2.402 ± 0.037
0.21HisCys: 0.21 ± 0.012
1.321HisAsp: 1.321 ± 0.029
0.947HisGlu: 0.947 ± 0.025
0.909HisPhe: 0.909 ± 0.024
2.071HisGly: 2.071 ± 0.04
0.599HisHis: 0.599 ± 0.02
1.084HisIle: 1.084 ± 0.024
0.496HisLys: 0.496 ± 0.017
1.984HisLeu: 1.984 ± 0.037
0.547HisMet: 0.547 ± 0.017
0.477HisAsn: 0.477 ± 0.017
1.307HisPro: 1.307 ± 0.028
0.642HisGln: 0.642 ± 0.022
1.463HisArg: 1.463 ± 0.034
0.952HisSer: 0.952 ± 0.023
0.616HisThr: 0.616 ± 0.02
1.65HisVal: 1.65 ± 0.035
0.418HisTrp: 0.418 ± 0.018
0.656HisTyr: 0.656 ± 0.021
0.0HisXaa: 0.0 ± 0.0
Ile
7.944IleAla: 7.944 ± 0.073
0.45IleCys: 0.45 ± 0.017
4.151IleAsp: 4.151 ± 0.048
3.335IleGlu: 3.335 ± 0.055
1.729IlePhe: 1.729 ± 0.034
5.241IleGly: 5.241 ± 0.063
0.981IleHis: 0.981 ± 0.024
2.339IleIle: 2.339 ± 0.041
1.277IleLys: 1.277 ± 0.03
4.422IleLeu: 4.422 ± 0.058
0.999IleMet: 0.999 ± 0.024
1.411IleAsn: 1.411 ± 0.031
2.272IlePro: 2.272 ± 0.035
1.246IleGln: 1.246 ± 0.028
3.253IleArg: 3.253 ± 0.047
2.85IleSer: 2.85 ± 0.049
2.532IleThr: 2.532 ± 0.044
4.212IleVal: 4.212 ± 0.048
0.675IleTrp: 0.675 ± 0.023
1.067IleTyr: 1.067 ± 0.026
0.0IleXaa: 0.0 ± 0.0
Lys
4.409LysAla: 4.409 ± 0.064
0.15LysCys: 0.15 ± 0.01
1.736LysAsp: 1.736 ± 0.037
1.287LysGlu: 1.287 ± 0.032
0.834LysPhe: 0.834 ± 0.022
2.902LysGly: 2.902 ± 0.04
0.486LysHis: 0.486 ± 0.019
1.631LysIle: 1.631 ± 0.031
1.091LysLys: 1.091 ± 0.032
3.138LysLeu: 3.138 ± 0.043
0.776LysMet: 0.776 ± 0.022
0.759LysAsn: 0.759 ± 0.026
2.082LysPro: 2.082 ± 0.04
0.962LysGln: 0.962 ± 0.022
2.196LysArg: 2.196 ± 0.036
1.646LysSer: 1.646 ± 0.036
1.721LysThr: 1.721 ± 0.031
2.269LysVal: 2.269 ± 0.037
0.407LysTrp: 0.407 ± 0.019
0.585LysTyr: 0.585 ± 0.018
0.0LysXaa: 0.0 ± 0.0
Leu
12.995LeuAla: 12.995 ± 0.111
0.888LeuCys: 0.888 ± 0.021
5.996LeuAsp: 5.996 ± 0.067
4.564LeuGlu: 4.564 ± 0.061
3.742LeuPhe: 3.742 ± 0.049
7.861LeuGly: 7.861 ± 0.082
1.911LeuHis: 1.911 ± 0.035
5.13LeuIle: 5.13 ± 0.057
3.064LeuLys: 3.064 ± 0.042
9.691LeuLeu: 9.691 ± 0.097
2.246LeuMet: 2.246 ± 0.036
2.545LeuAsn: 2.545 ± 0.038
5.811LeuPro: 5.811 ± 0.062
2.515LeuGln: 2.515 ± 0.041
6.721LeuArg: 6.721 ± 0.061
6.486LeuSer: 6.486 ± 0.071
5.867LeuThr: 5.867 ± 0.065
6.504LeuVal: 6.504 ± 0.071
1.305LeuTrp: 1.305 ± 0.028
2.185LeuTyr: 2.185 ± 0.04
0.0LeuXaa: 0.0 ± 0.0
Met
3.552MetAla: 3.552 ± 0.047
0.147MetCys: 0.147 ± 0.01
1.248MetAsp: 1.248 ± 0.025
1.084MetGlu: 1.084 ± 0.027
0.663MetPhe: 0.663 ± 0.021
2.012MetGly: 2.012 ± 0.036
0.436MetHis: 0.436 ± 0.015
1.415MetIle: 1.415 ± 0.03
0.98MetLys: 0.98 ± 0.025
2.769MetLeu: 2.769 ± 0.043
0.726MetMet: 0.726 ± 0.023
0.666MetAsn: 0.666 ± 0.019
1.518MetPro: 1.518 ± 0.03
0.824MetGln: 0.824 ± 0.023
1.861MetArg: 1.861 ± 0.034
1.508MetSer: 1.508 ± 0.031
1.869MetThr: 1.869 ± 0.038
1.703MetVal: 1.703 ± 0.034
0.215MetTrp: 0.215 ± 0.011
0.252MetTyr: 0.252 ± 0.012
0.0MetXaa: 0.0 ± 0.0
Asn
3.343AsnAla: 3.343 ± 0.045
0.224AsnCys: 0.224 ± 0.012
1.624AsnAsp: 1.624 ± 0.049
1.099AsnGlu: 1.099 ± 0.027
0.982AsnPhe: 0.982 ± 0.03
2.699AsnGly: 2.699 ± 0.055
0.521AsnHis: 0.521 ± 0.017
1.464AsnIle: 1.464 ± 0.034
0.689AsnLys: 0.689 ± 0.026
2.491AsnLeu: 2.491 ± 0.049
0.639AsnMet: 0.639 ± 0.018
0.787AsnAsn: 0.787 ± 0.026
1.747AsnPro: 1.747 ± 0.034
0.796AsnGln: 0.796 ± 0.022
1.856AsnArg: 1.856 ± 0.033
1.406AsnSer: 1.406 ± 0.034
0.997AsnThr: 0.997 ± 0.029
1.896AsnVal: 1.896 ± 0.044
0.504AsnTrp: 0.504 ± 0.018
0.794AsnTyr: 0.794 ± 0.025
0.0AsnXaa: 0.0 ± 0.0
Pro
6.785ProAla: 6.785 ± 0.069
0.335ProCys: 0.335 ± 0.016
4.114ProAsp: 4.114 ± 0.052
3.075ProGlu: 3.075 ± 0.045
1.947ProPhe: 1.947 ± 0.035
4.632ProGly: 4.632 ± 0.064
1.101ProHis: 1.101 ± 0.023
2.561ProIle: 2.561 ± 0.04
1.533ProLys: 1.533 ± 0.033
4.828ProLeu: 4.828 ± 0.058
1.314ProMet: 1.314 ± 0.033
1.272ProAsn: 1.272 ± 0.031
2.67ProPro: 2.67 ± 0.062
1.857ProGln: 1.857 ± 0.035
2.92ProArg: 2.92 ± 0.041
2.848ProSer: 2.848 ± 0.042
2.738ProThr: 2.738 ± 0.043
4.396ProVal: 4.396 ± 0.056
0.732ProTrp: 0.732 ± 0.019
1.153ProTyr: 1.153 ± 0.028
0.0ProXaa: 0.0 ± 0.0
Gln
4.617GlnAla: 4.617 ± 0.061
0.237GlnCys: 0.237 ± 0.012
1.753GlnAsp: 1.753 ± 0.034
1.398GlnGlu: 1.398 ± 0.029
1.059GlnPhe: 1.059 ± 0.026
2.77GlnGly: 2.77 ± 0.035
0.634GlnHis: 0.634 ± 0.019
1.9GlnIle: 1.9 ± 0.035
1.001GlnLys: 1.001 ± 0.024
3.045GlnLeu: 3.045 ± 0.047
0.942GlnMet: 0.942 ± 0.026
0.837GlnAsn: 0.837 ± 0.022
1.939GlnPro: 1.939 ± 0.035
1.317GlnGln: 1.317 ± 0.039
2.592GlnArg: 2.592 ± 0.042
2.014GlnSer: 2.014 ± 0.04
1.752GlnThr: 1.752 ± 0.033
2.344GlnVal: 2.344 ± 0.037
0.512GlnTrp: 0.512 ± 0.018
0.709GlnTyr: 0.709 ± 0.02
0.0GlnXaa: 0.0 ± 0.0
Arg
8.412ArgAla: 8.412 ± 0.083
0.521ArgCys: 0.521 ± 0.017
4.428ArgAsp: 4.428 ± 0.05
3.57ArgGlu: 3.57 ± 0.058
3.02ArgPhe: 3.02 ± 0.044
4.811ArgGly: 4.811 ± 0.059
1.797ArgHis: 1.797 ± 0.033
4.198ArgIle: 4.198 ± 0.053
2.189ArgLys: 2.189 ± 0.038
7.866ArgLeu: 7.866 ± 0.07
1.93ArgMet: 1.93 ± 0.032
1.937ArgAsn: 1.937 ± 0.037
3.694ArgPro: 3.694 ± 0.055
2.643ArgGln: 2.643 ± 0.045
5.486ArgArg: 5.486 ± 0.077
3.687ArgSer: 3.687 ± 0.06
3.558ArgThr: 3.558 ± 0.044
4.403ArgVal: 4.403 ± 0.059
1.236ArgTrp: 1.236 ± 0.029
2.039ArgTyr: 2.039 ± 0.035
0.0ArgXaa: 0.0 ± 0.0
Ser
6.716SerAla: 6.716 ± 0.068
0.44SerCys: 0.44 ± 0.016
3.383SerAsp: 3.383 ± 0.04
2.398SerGlu: 2.398 ± 0.04
2.258SerPhe: 2.258 ± 0.041
5.529SerGly: 5.529 ± 0.066
1.081SerHis: 1.081 ± 0.029
2.939SerIle: 2.939 ± 0.048
1.515SerLys: 1.515 ± 0.03
5.552SerLeu: 5.552 ± 0.055
1.221SerMet: 1.221 ± 0.029
1.453SerAsn: 1.453 ± 0.035
2.899SerPro: 2.899 ± 0.045
1.686SerGln: 1.686 ± 0.032
3.57SerArg: 3.57 ± 0.047
3.01SerSer: 3.01 ± 0.054
2.718SerThr: 2.718 ± 0.043
3.722SerVal: 3.722 ± 0.054
0.837SerTrp: 0.837 ± 0.022
1.549SerTyr: 1.549 ± 0.034
0.0SerXaa: 0.0 ± 0.0
Thr
6.227ThrAla: 6.227 ± 0.066
0.375ThrCys: 0.375 ± 0.017
3.185ThrAsp: 3.185 ± 0.046
2.148ThrGlu: 2.148 ± 0.037
1.72ThrPhe: 1.72 ± 0.032
5.486ThrGly: 5.486 ± 0.073
1.012ThrHis: 1.012 ± 0.027
3.028ThrIle: 3.028 ± 0.039
1.377ThrLys: 1.377 ± 0.027
5.986ThrLeu: 5.986 ± 0.071
1.17ThrMet: 1.17 ± 0.029
1.339ThrAsn: 1.339 ± 0.036
3.556ThrPro: 3.556 ± 0.041
1.601ThrGln: 1.601 ± 0.033
3.356ThrArg: 3.356 ± 0.046
2.772ThrSer: 2.772 ± 0.047
2.716ThrThr: 2.716 ± 0.05
3.969ThrVal: 3.969 ± 0.052
0.606ThrTrp: 0.606 ± 0.018
1.241ThrTyr: 1.241 ± 0.034
0.0ThrXaa: 0.0 ± 0.0
Val
9.205ValAla: 9.205 ± 0.085
0.478ValCys: 0.478 ± 0.017
4.494ValAsp: 4.494 ± 0.052
4.321ValGlu: 4.321 ± 0.061
1.833ValPhe: 1.833 ± 0.032
5.542ValGly: 5.542 ± 0.062
1.402ValHis: 1.402 ± 0.031
3.475ValIle: 3.475 ± 0.053
2.156ValLys: 2.156 ± 0.041
5.782ValLeu: 5.782 ± 0.064
1.658ValMet: 1.658 ± 0.035
2.072ValAsn: 2.072 ± 0.041
3.646ValPro: 3.646 ± 0.052
2.221ValGln: 2.221 ± 0.036
4.961ValArg: 4.961 ± 0.055
3.97ValSer: 3.97 ± 0.048
4.428ValThr: 4.428 ± 0.059
4.764ValVal: 4.764 ± 0.057
0.79ValTrp: 0.79 ± 0.021
1.384ValTyr: 1.384 ± 0.032
0.0ValXaa: 0.0 ± 0.0
Trp
1.457TrpAla: 1.457 ± 0.031
0.115TrpCys: 0.115 ± 0.009
0.797TrpAsp: 0.797 ± 0.024
0.598TrpGlu: 0.598 ± 0.018
0.538TrpPhe: 0.538 ± 0.018
1.003TrpGly: 1.003 ± 0.025
0.379TrpHis: 0.379 ± 0.016
0.717TrpIle: 0.717 ± 0.02
0.537TrpLys: 0.537 ± 0.018
1.715TrpLeu: 1.715 ± 0.034
0.378TrpMet: 0.378 ± 0.015
0.504TrpAsn: 0.504 ± 0.018
0.73TrpPro: 0.73 ± 0.019
0.612TrpGln: 0.612 ± 0.017
1.367TrpArg: 1.367 ± 0.034
0.972TrpSer: 0.972 ± 0.025
0.9TrpThr: 0.9 ± 0.023
0.838TrpVal: 0.838 ± 0.023
0.247TrpTrp: 0.247 ± 0.014
0.314TrpTyr: 0.314 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.882TyrAla: 2.882 ± 0.041
0.254TyrCys: 0.254 ± 0.013
1.725TyrAsp: 1.725 ± 0.032
1.169TyrGlu: 1.169 ± 0.026
0.89TyrPhe: 0.89 ± 0.024
2.268TyrGly: 2.268 ± 0.043
0.571TyrHis: 0.571 ± 0.02
0.926TyrIle: 0.926 ± 0.025
0.653TyrLys: 0.653 ± 0.021
2.248TyrLeu: 2.248 ± 0.035
0.478TyrMet: 0.478 ± 0.015
0.731TyrAsn: 0.731 ± 0.02
1.095TyrPro: 1.095 ± 0.024
0.806TyrGln: 0.806 ± 0.022
2.031TyrArg: 2.031 ± 0.034
1.349TyrSer: 1.349 ± 0.034
1.079TyrThr: 1.079 ± 0.03
1.643TyrVal: 1.643 ± 0.031
0.378TyrTrp: 0.378 ± 0.016
0.758TyrTyr: 0.758 ± 0.021
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5343 proteins (1701863 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski