Phaseolus angularis (Azuki bean) (Vigna angularis)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 33730 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0L9TXU1|A0A0L9TXU1_PHAAN AP2/ERF domain-containing protein OS=Phaseolus angularis OX=3914 GN=LR48_Vigan02g136700 PE=4 SV=1
MM1 pKa = 7.32EE2 pKa = 5.44RR3 pKa = 11.84KK4 pKa = 9.66KK5 pKa = 10.64PLDD8 pKa = 3.61FSSYY12 pKa = 10.55FCFEE16 pKa = 3.89ASGDD20 pKa = 4.0SEE22 pKa = 4.37EE23 pKa = 4.7AHH25 pKa = 6.9PDD27 pKa = 3.49DD28 pKa = 6.21PIFACEE34 pKa = 3.7MTRR37 pKa = 11.84AYY39 pKa = 10.54GDD41 pKa = 4.44DD42 pKa = 4.01EE43 pKa = 6.11NDD45 pKa = 4.32DD46 pKa = 4.25ALSCNFEE53 pKa = 3.93GSGAFDD59 pKa = 3.03GAVFDD64 pKa = 4.45EE65 pKa = 5.32EE66 pKa = 5.16EE67 pKa = 4.47NEE69 pKa = 4.62CCDD72 pKa = 5.17DD73 pKa = 4.35DD74 pKa = 6.63HH75 pKa = 9.25DD76 pKa = 5.69DD77 pKa = 4.82EE78 pKa = 4.64IAKK81 pKa = 8.08VTQDD85 pKa = 3.63EE86 pKa = 4.43KK87 pKa = 11.04SGVYY91 pKa = 10.3GMSYY95 pKa = 10.89CEE97 pKa = 4.5DD98 pKa = 3.9DD99 pKa = 4.92DD100 pKa = 4.55MEE102 pKa = 4.39EE103 pKa = 4.04EE104 pKa = 4.3HH105 pKa = 6.65MKK107 pKa = 10.47SHH109 pKa = 6.27VSFDD113 pKa = 3.62SGHH116 pKa = 5.85EE117 pKa = 4.1FVDD120 pKa = 3.71EE121 pKa = 4.16MEE123 pKa = 4.39KK124 pKa = 10.71NRR126 pKa = 11.84LFWEE130 pKa = 4.55ACLASS135 pKa = 3.65

Molecular weight: 15.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0L9T8I7|A0A0L9T8I7_PHAAN Cytokinin riboside 5'-monophosphate phosphoribohydrolase OS=Phaseolus angularis OX=3914 GN=LR48_Vigan345s000900 PE=3 SV=1
MM1 pKa = 7.24HH2 pKa = 7.21FVNFVHH8 pKa = 6.87IVAFVTFVNFVHH20 pKa = 7.18FIHH23 pKa = 6.56FVSSSTSSTSLFSSTLSNSSTSSTLSTWTTLSTSSNSFNSSTSSTSSISSTSSVHH78 pKa = 5.93PLPPVRR84 pKa = 11.84PLPPVRR90 pKa = 11.84SLRR93 pKa = 11.84HH94 pKa = 5.25FNHH97 pKa = 6.3FVSSSPSSSSSTSSTSSTSSTSSTSSTSAVRR128 pKa = 11.84PLRR131 pKa = 11.84PLHH134 pKa = 6.63PLRR137 pKa = 11.84QLRR140 pKa = 11.84PLRR143 pKa = 11.84RR144 pKa = 11.84LRR146 pKa = 11.84PLRR149 pKa = 11.84PIRR152 pKa = 11.84QLRR155 pKa = 11.84SLRR158 pKa = 11.84PLCPLGPLRR167 pKa = 11.84QLRR170 pKa = 11.84QLPPLPPLRR179 pKa = 11.84PLPLLHH185 pKa = 6.99PLLRR189 pKa = 11.84PLRR192 pKa = 11.84PLHH195 pKa = 6.02EE196 pKa = 4.59LRR198 pKa = 11.84PLRR201 pKa = 11.84PLPQLRR207 pKa = 11.84PFRR210 pKa = 11.84PLRR213 pKa = 11.84ALRR216 pKa = 11.84PLRR219 pKa = 11.84QLPPLRR225 pKa = 11.84PLRR228 pKa = 11.84QRR230 pKa = 11.84RR231 pKa = 11.84PLRR234 pKa = 11.84PLRR237 pKa = 11.84QFIHH241 pKa = 6.61FLQFVHH247 pKa = 7.19FIHH250 pKa = 6.6FVSFVHH256 pKa = 6.53FVSSSTSSTSSTSSTSSTSSVRR278 pKa = 11.84PLPLVRR284 pKa = 11.84LLNPLRR290 pKa = 11.84QFVHH294 pKa = 6.41FVNFVHH300 pKa = 7.17FVHH303 pKa = 6.5SLNSVHH309 pKa = 6.54FVHH312 pKa = 7.14FVHH315 pKa = 6.94FVSSSPSSTPSTSSTSATSSTSLVRR340 pKa = 11.84PLPPLRR346 pKa = 11.84PLPPLPPLRR355 pKa = 11.84PLRR358 pKa = 4.15

Molecular weight: 40.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

33730

0

33730

12545473

49

9099

371.9

41.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.452 ± 0.018

1.83 ± 0.008

5.558 ± 0.035

7.204 ± 0.071

4.147 ± 0.012

6.429 ± 0.018

2.502 ± 0.008

5.069 ± 0.013

5.917 ± 0.015

9.359 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.387 ± 0.007

4.415 ± 0.015

4.919 ± 0.019

3.627 ± 0.011

5.419 ± 0.015

8.739 ± 0.025

4.933 ± 0.012

7.071 ± 0.039

1.301 ± 0.005

2.677 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski