Amino acid dipepetide frequency for Verticillium nonalfalfae

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	12.132AlaAla: 12.132 ± 0.099	1.15AlaCys: 1.15 ± 0.021	5.138AlaAsp: 5.138 ± 0.037	5.693AlaGlu: 5.693 ± 0.051	3.339AlaPhe: 3.339 ± 0.03	6.91AlaGly: 6.91 ± 0.049	2.054AlaHis: 2.054 ± 0.022	4.187AlaIle: 4.187 ± 0.033	4.262AlaLys: 4.262 ± 0.042	8.459AlaLeu: 8.459 ± 0.051	2.229AlaMet: 2.229 ± 0.025	3.125AlaAsn: 3.125 ± 0.03	5.982AlaPro: 5.982 ± 0.056	3.782AlaGln: 3.782 ± 0.033	5.767AlaArg: 5.767 ± 0.041	7.973AlaSer: 7.973 ± 0.048	5.881AlaThr: 5.881 ± 0.047	6.23AlaVal: 6.23 ± 0.04	1.342AlaTrp: 1.342 ± 0.018	2.24AlaTyr: 2.24 ± 0.022	0.0AlaXaa: 0.0 ± 0.0
Cys	0.979CysAla: 0.979 ± 0.017	0.23CysCys: 0.23 ± 0.012	0.672CysAsp: 0.672 ± 0.015	0.572CysGlu: 0.572 ± 0.013	0.49CysPhe: 0.49 ± 0.011	0.94CysGly: 0.94 ± 0.025	0.312CysHis: 0.312 ± 0.009	0.625CysIle: 0.625 ± 0.013	0.469CysLys: 0.469 ± 0.014	1.147CysLeu: 1.147 ± 0.02	0.248CysMet: 0.248 ± 0.007	0.424CysAsn: 0.424 ± 0.011	0.638CysPro: 0.638 ± 0.017	0.411CysGln: 0.411 ± 0.012	0.729CysArg: 0.729 ± 0.014	0.799CysSer: 0.799 ± 0.015	0.667CysThr: 0.667 ± 0.018	0.792CysVal: 0.792 ± 0.024	0.185CysTrp: 0.185 ± 0.006	0.325CysTyr: 0.325 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	5.797AspAla: 5.797 ± 0.042	0.605AspCys: 0.605 ± 0.012	5.043AspAsp: 5.043 ± 0.053	4.697AspGlu: 4.697 ± 0.046	2.237AspPhe: 2.237 ± 0.019	4.535AspGly: 4.535 ± 0.035	1.29AspHis: 1.29 ± 0.016	2.788AspIle: 2.788 ± 0.026	2.445AspLys: 2.445 ± 0.025	5.063AspLeu: 5.063 ± 0.039	1.361AspMet: 1.361 ± 0.018	1.858AspAsn: 1.858 ± 0.022	3.348AspPro: 3.348 ± 0.028	1.857AspGln: 1.857 ± 0.021	3.204AspArg: 3.204 ± 0.034	3.899AspSer: 3.899 ± 0.031	2.971AspThr: 2.971 ± 0.029	4.022AspVal: 4.022 ± 0.03	0.916AspTrp: 0.916 ± 0.012	1.542AspTyr: 1.542 ± 0.021	0.0AspXaa: 0.0 ± 0.0
Glu	6.088GluAla: 6.088 ± 0.049	0.571GluCys: 0.571 ± 0.013	4.255GluAsp: 4.255 ± 0.039	4.992GluGlu: 4.992 ± 0.059	1.806GluPhe: 1.806 ± 0.022	3.88GluGly: 3.88 ± 0.032	1.356GluHis: 1.356 ± 0.016	2.598GluIle: 2.598 ± 0.026	3.305GluLys: 3.305 ± 0.033	4.963GluLeu: 4.963 ± 0.039	1.404GluMet: 1.404 ± 0.02	1.941GluAsn: 1.941 ± 0.019	2.981GluPro: 2.981 ± 0.051	2.344GluGln: 2.344 ± 0.026	3.871GluArg: 3.871 ± 0.034	3.876GluSer: 3.876 ± 0.032	3.542GluThr: 3.542 ± 0.03	3.578GluVal: 3.578 ± 0.034	0.812GluTrp: 0.812 ± 0.014	1.511GluTyr: 1.511 ± 0.019	0.0GluXaa: 0.0 ± 0.0
Phe	3.34PheAla: 3.34 ± 0.03	0.53PheCys: 0.53 ± 0.011	2.374PheAsp: 2.374 ± 0.024	2.109PheGlu: 2.109 ± 0.021	1.635PhePhe: 1.635 ± 0.025	2.942PheGly: 2.942 ± 0.03	0.862PheHis: 0.862 ± 0.013	1.643PheIle: 1.643 ± 0.02	1.393PheLys: 1.393 ± 0.019	3.278PheLeu: 3.278 ± 0.035	0.78PheMet: 0.78 ± 0.014	1.377PheAsn: 1.377 ± 0.016	1.846PhePro: 1.846 ± 0.023	1.295PheGln: 1.295 ± 0.018	1.94PheArg: 1.94 ± 0.023	2.694PheSer: 2.694 ± 0.021	2.071PheThr: 2.071 ± 0.021	2.453PheVal: 2.453 ± 0.026	0.637PheTrp: 0.637 ± 0.013	1.011PheTyr: 1.011 ± 0.016	0.0PheXaa: 0.0 ± 0.0
Gly	6.33GlyAla: 6.33 ± 0.05	0.85GlyCys: 0.85 ± 0.016	4.028GlyAsp: 4.028 ± 0.032	3.704GlyGlu: 3.704 ± 0.03	2.842GlyPhe: 2.842 ± 0.028	6.738GlyGly: 6.738 ± 0.086	1.819GlyHis: 1.819 ± 0.025	3.348GlyIle: 3.348 ± 0.029	3.289GlyLys: 3.289 ± 0.035	6.299GlyLeu: 6.299 ± 0.047	1.627GlyMet: 1.627 ± 0.02	2.578GlyAsn: 2.578 ± 0.031	3.738GlyPro: 3.738 ± 0.036	2.734GlyGln: 2.734 ± 0.033	4.494GlyArg: 4.494 ± 0.039	5.765GlySer: 5.765 ± 0.043	4.156GlyThr: 4.156 ± 0.039	4.636GlyVal: 4.636 ± 0.039	1.196GlyTrp: 1.196 ± 0.018	2.054GlyTyr: 2.054 ± 0.024	0.0GlyXaa: 0.0 ± 0.0
His	2.155HisAla: 2.155 ± 0.024	0.304HisCys: 0.304 ± 0.009	1.458HisAsp: 1.458 ± 0.017	1.326HisGlu: 1.326 ± 0.017	0.897HisPhe: 0.897 ± 0.014	1.844HisGly: 1.844 ± 0.022	0.893HisHis: 0.893 ± 0.017	1.128HisIle: 1.128 ± 0.017	0.864HisLys: 0.864 ± 0.014	2.186HisLeu: 2.186 ± 0.024	0.516HisMet: 0.516 ± 0.011	0.809HisAsn: 0.809 ± 0.014	1.605HisPro: 1.605 ± 0.021	1.029HisGln: 1.029 ± 0.017	1.562HisArg: 1.562 ± 0.018	1.669HisSer: 1.669 ± 0.024	1.241HisThr: 1.241 ± 0.016	1.573HisVal: 1.573 ± 0.02	0.33HisTrp: 0.33 ± 0.009	0.667HisTyr: 0.667 ± 0.012	0.0HisXaa: 0.0 ± 0.0
Ile	4.144IleAla: 4.144 ± 0.029	0.661IleCys: 0.661 ± 0.017	2.737IleAsp: 2.737 ± 0.029	2.598IleGlu: 2.598 ± 0.025	1.782IlePhe: 1.782 ± 0.018	3.008IleGly: 3.008 ± 0.027	1.067IleHis: 1.067 ± 0.015	2.251IleIle: 2.251 ± 0.026	1.947IleLys: 1.947 ± 0.025	4.05IleLeu: 4.05 ± 0.033	1.009IleMet: 1.009 ± 0.017	1.627IleAsn: 1.627 ± 0.022	2.683IlePro: 2.683 ± 0.029	1.634IleGln: 1.634 ± 0.022	2.583IleArg: 2.583 ± 0.026	3.258IleSer: 3.258 ± 0.027	2.594IleThr: 2.594 ± 0.023	3.09IleVal: 3.09 ± 0.03	0.681IleTrp: 0.681 ± 0.014	1.189IleTyr: 1.189 ± 0.016	0.0IleXaa: 0.0 ± 0.0
Lys	4.47LysAla: 4.47 ± 0.038	0.45LysCys: 0.45 ± 0.012	2.623LysAsp: 2.623 ± 0.025	2.939LysGlu: 2.939 ± 0.034	1.346LysPhe: 1.346 ± 0.019	2.863LysGly: 2.863 ± 0.029	1.056LysHis: 1.056 ± 0.016	1.98LysIle: 1.98 ± 0.024	3.255LysLys: 3.255 ± 0.068	3.726LysLeu: 3.726 ± 0.032	0.964LysMet: 0.964 ± 0.014	1.571LysAsn: 1.571 ± 0.021	2.676LysPro: 2.676 ± 0.03	1.711LysGln: 1.711 ± 0.021	3.191LysArg: 3.191 ± 0.03	3.086LysSer: 3.086 ± 0.027	2.864LysThr: 2.864 ± 0.027	2.706LysVal: 2.706 ± 0.025	0.616LysTrp: 0.616 ± 0.012	1.202LysTyr: 1.202 ± 0.019	0.0LysXaa: 0.0 ± 0.0
Leu	8.657LeuAla: 8.657 ± 0.056	1.082LeuCys: 1.082 ± 0.016	5.179LeuAsp: 5.179 ± 0.038	5.205LeuGlu: 5.205 ± 0.041	3.208LeuPhe: 3.208 ± 0.033	6.002LeuGly: 6.002 ± 0.044	2.164LeuHis: 2.164 ± 0.027	3.543LeuIle: 3.543 ± 0.035	3.759LeuLys: 3.759 ± 0.033	8.026LeuLeu: 8.026 ± 0.054	1.757LeuMet: 1.757 ± 0.02	2.91LeuAsn: 2.91 ± 0.026	5.497LeuPro: 5.497 ± 0.044	3.593LeuGln: 3.593 ± 0.033	5.78LeuArg: 5.78 ± 0.042	6.668LeuSer: 6.668 ± 0.041	4.932LeuThr: 4.932 ± 0.033	5.528LeuVal: 5.528 ± 0.04	1.176LeuTrp: 1.176 ± 0.016	2.174LeuTyr: 2.174 ± 0.024	0.0LeuXaa: 0.0 ± 0.0
Met	2.49MetAla: 2.49 ± 0.025	0.229MetCys: 0.229 ± 0.007	1.251MetAsp: 1.251 ± 0.017	1.184MetGlu: 1.184 ± 0.017	0.743MetPhe: 0.743 ± 0.015	1.541MetGly: 1.541 ± 0.021	0.491MetHis: 0.491 ± 0.009	0.92MetIle: 0.92 ± 0.014	0.957MetLys: 0.957 ± 0.014	1.824MetLeu: 1.824 ± 0.023	0.584MetMet: 0.584 ± 0.013	0.762MetAsn: 0.762 ± 0.014	1.36MetPro: 1.36 ± 0.019	0.834MetGln: 0.834 ± 0.015	1.321MetArg: 1.321 ± 0.017	1.834MetSer: 1.834 ± 0.019	1.366MetThr: 1.366 ± 0.02	1.336MetVal: 1.336 ± 0.018	0.263MetTrp: 0.263 ± 0.007	0.515MetTyr: 0.515 ± 0.011	0.0MetXaa: 0.0 ± 0.0
Asn	3.16AsnAla: 3.16 ± 0.029	0.421AsnCys: 0.421 ± 0.01	1.958AsnAsp: 1.958 ± 0.022	1.849AsnGlu: 1.849 ± 0.022	1.311AsnPhe: 1.311 ± 0.018	2.982AsnGly: 2.982 ± 0.035	0.785AsnHis: 0.785 ± 0.013	1.776AsnIle: 1.776 ± 0.022	1.472AsnLys: 1.472 ± 0.019	2.998AsnLeu: 2.998 ± 0.027	0.817AsnMet: 0.817 ± 0.014	1.398AsnAsn: 1.398 ± 0.028	2.321AsnPro: 2.321 ± 0.024	1.184AsnGln: 1.184 ± 0.016	1.836AsnArg: 1.836 ± 0.02	2.371AsnSer: 2.371 ± 0.025	2.055AsnThr: 2.055 ± 0.02	2.265AsnVal: 2.265 ± 0.024	0.522AsnTrp: 0.522 ± 0.012	0.954AsnTyr: 0.954 ± 0.014	0.0AsnXaa: 0.0 ± 0.0
Pro	6.428ProAla: 6.428 ± 0.062	0.525ProCys: 0.525 ± 0.015	3.359ProAsp: 3.359 ± 0.031	3.835ProGlu: 3.835 ± 0.04	2.029ProPhe: 2.029 ± 0.023	4.382ProGly: 4.382 ± 0.046	1.345ProHis: 1.345 ± 0.017	2.31ProIle: 2.31 ± 0.024	2.538ProLys: 2.538 ± 0.028	4.803ProLeu: 4.803 ± 0.041	1.125ProMet: 1.125 ± 0.017	2.044ProAsn: 2.044 ± 0.024	5.495ProPro: 5.495 ± 0.084	2.512ProGln: 2.512 ± 0.031	3.631ProArg: 3.631 ± 0.04	5.934ProSer: 5.934 ± 0.055	4.093ProThr: 4.093 ± 0.04	3.872ProVal: 3.872 ± 0.038	0.768ProTrp: 0.768 ± 0.016	1.439ProTyr: 1.439 ± 0.02	0.0ProXaa: 0.0 ± 0.0
Gln	3.793GlnAla: 3.793 ± 0.035	0.424GlnCys: 0.424 ± 0.01	2.076GlnAsp: 2.076 ± 0.023	2.177GlnGlu: 2.177 ± 0.028	1.186GlnPhe: 1.186 ± 0.017	2.542GlnGly: 2.542 ± 0.027	1.165GlnHis: 1.165 ± 0.021	1.667GlnIle: 1.667 ± 0.018	1.776GlnLys: 1.776 ± 0.022	3.266GlnLeu: 3.266 ± 0.027	0.876GlnMet: 0.876 ± 0.015	1.383GlnAsn: 1.383 ± 0.017	2.645GlnPro: 2.645 ± 0.034	2.963GlnGln: 2.963 ± 0.068	2.633GlnArg: 2.633 ± 0.027	2.87GlnSer: 2.87 ± 0.027	2.34GlnThr: 2.34 ± 0.023	2.196GlnVal: 2.196 ± 0.022	0.558GlnTrp: 0.558 ± 0.012	1.095GlnTyr: 1.095 ± 0.017	0.0GlnXaa: 0.0 ± 0.0
Arg	5.453ArgAla: 5.453 ± 0.041	0.697ArgCys: 0.697 ± 0.012	3.693ArgAsp: 3.693 ± 0.037	3.77ArgGlu: 3.77 ± 0.035	2.129ArgPhe: 2.129 ± 0.019	4.025ArgGly: 4.025 ± 0.036	1.697ArgHis: 1.697 ± 0.023	2.71ArgIle: 2.71 ± 0.024	3.235ArgLys: 3.235 ± 0.034	5.399ArgLeu: 5.399 ± 0.038	1.321ArgMet: 1.321 ± 0.013	2.15ArgAsn: 2.15 ± 0.021	3.78ArgPro: 3.78 ± 0.034	2.744ArgGln: 2.744 ± 0.028	5.355ArgArg: 5.355 ± 0.049	4.659ArgSer: 4.659 ± 0.043	3.377ArgThr: 3.377 ± 0.03	3.469ArgVal: 3.469 ± 0.029	0.967ArgTrp: 0.967 ± 0.014	1.592ArgTyr: 1.592 ± 0.019	0.0ArgXaa: 0.0 ± 0.0
Ser	7.055SerAla: 7.055 ± 0.047	0.794SerCys: 0.794 ± 0.014	4.129SerAsp: 4.129 ± 0.033	3.766SerGlu: 3.766 ± 0.032	2.861SerPhe: 2.861 ± 0.025	5.533SerGly: 5.533 ± 0.037	1.877SerHis: 1.877 ± 0.024	3.534SerIle: 3.534 ± 0.03	3.282SerLys: 3.282 ± 0.031	6.716SerLeu: 6.716 ± 0.047	1.669SerMet: 1.669 ± 0.021	2.698SerAsn: 2.698 ± 0.028	5.396SerPro: 5.396 ± 0.056	3.019SerGln: 3.019 ± 0.033	4.876SerArg: 4.876 ± 0.037	8.065SerSer: 8.065 ± 0.069	5.211SerThr: 5.211 ± 0.04	4.505SerVal: 4.505 ± 0.036	1.085SerTrp: 1.085 ± 0.015	1.893SerTyr: 1.893 ± 0.023	0.0SerXaa: 0.0 ± 0.0
Thr	5.868ThrAla: 5.868 ± 0.042	0.744ThrCys: 0.744 ± 0.027	2.95ThrAsp: 2.95 ± 0.027	3.023ThrGlu: 3.023 ± 0.028	2.185ThrPhe: 2.185 ± 0.024	4.292ThrGly: 4.292 ± 0.039	1.302ThrHis: 1.302 ± 0.018	2.903ThrIle: 2.903 ± 0.026	2.534ThrLys: 2.534 ± 0.026	5.142ThrLeu: 5.142 ± 0.038	1.221ThrMet: 1.221 ± 0.017	2.028ThrAsn: 2.028 ± 0.022	4.589ThrPro: 4.589 ± 0.04	2.086ThrGln: 2.086 ± 0.023	3.268ThrArg: 3.268 ± 0.027	5.161ThrSer: 5.161 ± 0.039	4.521ThrThr: 4.521 ± 0.054	3.831ThrVal: 3.831 ± 0.037	0.889ThrTrp: 0.889 ± 0.016	1.522ThrTyr: 1.522 ± 0.02	0.0ThrXaa: 0.0 ± 0.0
Val	6.065ValAla: 6.065 ± 0.039	0.822ValCys: 0.822 ± 0.025	3.967ValAsp: 3.967 ± 0.031	3.948ValGlu: 3.948 ± 0.032	2.514ValPhe: 2.514 ± 0.028	4.23ValGly: 4.23 ± 0.037	1.415ValHis: 1.415 ± 0.019	2.788ValIle: 2.788 ± 0.027	2.819ValLys: 2.819 ± 0.032	5.738ValLeu: 5.738 ± 0.043	1.357ValMet: 1.357 ± 0.015	2.163ValAsn: 2.163 ± 0.024	3.854ValPro: 3.854 ± 0.035	2.315ValGln: 2.315 ± 0.024	3.66ValArg: 3.66 ± 0.03	4.564ValSer: 4.564 ± 0.032	3.754ValThr: 3.754 ± 0.033	4.542ValVal: 4.542 ± 0.041	0.903ValTrp: 0.903 ± 0.014	1.682ValTyr: 1.682 ± 0.02	0.0ValXaa: 0.0 ± 0.0
Trp	1.254TrpAla: 1.254 ± 0.018	0.19TrpCys: 0.19 ± 0.006	0.93TrpAsp: 0.93 ± 0.018	0.807TrpGlu: 0.807 ± 0.014	0.556TrpPhe: 0.556 ± 0.011	0.991TrpGly: 0.991 ± 0.016	0.386TrpHis: 0.386 ± 0.01	0.69TrpIle: 0.69 ± 0.012	0.715TrpLys: 0.715 ± 0.012	1.332TrpLeu: 1.332 ± 0.019	0.363TrpMet: 0.363 ± 0.008	0.589TrpAsn: 0.589 ± 0.012	0.643TrpPro: 0.643 ± 0.011	0.597TrpGln: 0.597 ± 0.012	0.957TrpArg: 0.957 ± 0.016	1.028TrpSer: 1.028 ± 0.016	0.944TrpThr: 0.944 ± 0.014	0.889TrpVal: 0.889 ± 0.013	0.286TrpTrp: 0.286 ± 0.008	0.418TrpTyr: 0.418 ± 0.009	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.219TyrAla: 2.219 ± 0.022	0.376TyrCys: 0.376 ± 0.009	1.66TyrAsp: 1.66 ± 0.021	1.482TyrGlu: 1.482 ± 0.021	1.113TyrPhe: 1.113 ± 0.017	2.046TyrGly: 2.046 ± 0.029	0.72TyrHis: 0.72 ± 0.011	1.204TyrIle: 1.204 ± 0.017	0.98TyrLys: 0.98 ± 0.015	2.425TyrLeu: 2.425 ± 0.027	0.576TyrMet: 0.576 ± 0.011	1.002TyrAsn: 1.002 ± 0.016	1.388TyrPro: 1.388 ± 0.02	0.984TyrGln: 0.984 ± 0.015	1.566TyrArg: 1.566 ± 0.024	1.787TyrSer: 1.787 ± 0.022	1.479TyrThr: 1.479 ± 0.022	1.586TyrVal: 1.586 ± 0.021	0.434TyrTrp: 0.434 ± 0.009	0.833TyrTyr: 0.833 ± 0.016	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 9427 proteins (4738719 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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