Amino acid dipepetide frequency for Rhizobium phage RHEph09

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.466AlaAla: 12.466 ± 1.486
0.85AlaCys: 0.85 ± 0.278
6.729AlaAsp: 6.729 ± 0.807
5.383AlaGlu: 5.383 ± 0.783
3.967AlaPhe: 3.967 ± 0.509
8.5AlaGly: 8.5 ± 0.901
1.842AlaHis: 1.842 ± 0.528
4.533AlaIle: 4.533 ± 0.567
5.737AlaLys: 5.737 ± 0.532
7.862AlaLeu: 7.862 ± 0.682
2.479AlaMet: 2.479 ± 0.342
4.392AlaAsn: 4.392 ± 0.512
2.833AlaPro: 2.833 ± 0.383
4.462AlaGln: 4.462 ± 0.658
5.383AlaArg: 5.383 ± 0.523
5.383AlaSer: 5.383 ± 0.871
5.1AlaThr: 5.1 ± 0.738
6.658AlaVal: 6.658 ± 0.97
1.062AlaTrp: 1.062 ± 0.265
4.037AlaTyr: 4.037 ± 0.586
0.0AlaXaa: 0.0 ± 0.0
Cys
0.567CysAla: 0.567 ± 0.184
0.142CysCys: 0.142 ± 0.102
0.496CysAsp: 0.496 ± 0.173
0.496CysGlu: 0.496 ± 0.165
0.283CysPhe: 0.283 ± 0.151
0.921CysGly: 0.921 ± 0.275
0.425CysHis: 0.425 ± 0.165
0.708CysIle: 0.708 ± 0.239
0.354CysLys: 0.354 ± 0.173
0.567CysLeu: 0.567 ± 0.168
0.425CysMet: 0.425 ± 0.183
0.283CysAsn: 0.283 ± 0.14
0.637CysPro: 0.637 ± 0.261
0.142CysGln: 0.142 ± 0.142
0.354CysArg: 0.354 ± 0.197
0.354CysSer: 0.354 ± 0.164
0.567CysThr: 0.567 ± 0.21
0.637CysVal: 0.637 ± 0.242
0.212CysTrp: 0.212 ± 0.111
0.283CysTyr: 0.283 ± 0.149
0.0CysXaa: 0.0 ± 0.0
Asp
6.304AspAla: 6.304 ± 0.76
0.567AspCys: 0.567 ± 0.19
3.754AspAsp: 3.754 ± 0.453
3.754AspGlu: 3.754 ± 0.422
1.983AspPhe: 1.983 ± 0.292
5.525AspGly: 5.525 ± 0.698
1.062AspHis: 1.062 ± 0.382
4.25AspIle: 4.25 ± 0.389
3.896AspLys: 3.896 ± 0.678
4.887AspLeu: 4.887 ± 0.502
1.275AspMet: 1.275 ± 0.263
2.125AspAsn: 2.125 ± 0.484
3.967AspPro: 3.967 ± 0.725
2.337AspGln: 2.337 ± 0.313
4.108AspArg: 4.108 ± 0.723
2.621AspSer: 2.621 ± 0.458
3.754AspThr: 3.754 ± 0.602
3.329AspVal: 3.329 ± 0.522
1.346AspTrp: 1.346 ± 0.338
1.7AspTyr: 1.7 ± 0.325
0.0AspXaa: 0.0 ± 0.0
Glu
6.941GluAla: 6.941 ± 0.682
0.496GluCys: 0.496 ± 0.147
3.471GluAsp: 3.471 ± 0.503
3.329GluGlu: 3.329 ± 0.563
1.771GluPhe: 1.771 ± 0.34
3.117GluGly: 3.117 ± 0.563
0.992GluHis: 0.992 ± 0.345
2.975GluIle: 2.975 ± 0.51
3.471GluLys: 3.471 ± 0.425
4.462GluLeu: 4.462 ± 0.489
2.479GluMet: 2.479 ± 0.551
1.417GluAsn: 1.417 ± 0.394
0.992GluPro: 0.992 ± 0.201
3.471GluGln: 3.471 ± 0.593
3.612GluArg: 3.612 ± 0.62
2.408GluSer: 2.408 ± 0.382
2.762GluThr: 2.762 ± 0.509
3.825GluVal: 3.825 ± 0.511
1.133GluTrp: 1.133 ± 0.309
2.621GluTyr: 2.621 ± 0.443
0.0GluXaa: 0.0 ± 0.0
Phe
2.975PheAla: 2.975 ± 0.475
0.354PheCys: 0.354 ± 0.189
2.408PheAsp: 2.408 ± 0.389
2.196PheGlu: 2.196 ± 0.453
1.133PhePhe: 1.133 ± 0.345
2.55PheGly: 2.55 ± 0.49
0.992PheHis: 0.992 ± 0.314
1.983PheIle: 1.983 ± 0.443
1.842PheLys: 1.842 ± 0.435
2.621PheLeu: 2.621 ± 0.481
0.921PheMet: 0.921 ± 0.32
1.487PheAsn: 1.487 ± 0.394
1.133PhePro: 1.133 ± 0.321
1.275PheGln: 1.275 ± 0.286
1.558PheArg: 1.558 ± 0.429
2.054PheSer: 2.054 ± 0.363
1.629PheThr: 1.629 ± 0.372
1.912PheVal: 1.912 ± 0.336
0.708PheTrp: 0.708 ± 0.236
0.992PheTyr: 0.992 ± 0.254
0.0PheXaa: 0.0 ± 0.0
Gly
6.8GlyAla: 6.8 ± 0.705
0.637GlyCys: 0.637 ± 0.25
4.392GlyAsp: 4.392 ± 0.57
3.754GlyGlu: 3.754 ± 0.354
2.267GlyPhe: 2.267 ± 0.507
6.729GlyGly: 6.729 ± 0.737
0.85GlyHis: 0.85 ± 0.213
4.037GlyIle: 4.037 ± 0.488
5.171GlyLys: 5.171 ± 0.67
6.729GlyLeu: 6.729 ± 0.865
2.904GlyMet: 2.904 ± 0.415
3.329GlyAsn: 3.329 ± 0.479
2.55GlyPro: 2.55 ± 0.34
2.833GlyGln: 2.833 ± 0.489
4.321GlyArg: 4.321 ± 0.57
4.958GlySer: 4.958 ± 0.86
4.817GlyThr: 4.817 ± 0.735
4.746GlyVal: 4.746 ± 0.554
1.771GlyTrp: 1.771 ± 0.357
3.4GlyTyr: 3.4 ± 0.47
0.0GlyXaa: 0.0 ± 0.0
His
1.558HisAla: 1.558 ± 0.336
0.283HisCys: 0.283 ± 0.125
1.7HisAsp: 1.7 ± 0.486
1.346HisGlu: 1.346 ± 0.259
0.85HisPhe: 0.85 ± 0.277
1.062HisGly: 1.062 ± 0.274
0.708HisHis: 0.708 ± 0.227
1.204HisIle: 1.204 ± 0.316
0.637HisLys: 0.637 ± 0.207
2.337HisLeu: 2.337 ± 0.501
1.204HisMet: 1.204 ± 0.276
1.204HisAsn: 1.204 ± 0.242
1.062HisPro: 1.062 ± 0.275
0.354HisGln: 0.354 ± 0.161
1.275HisArg: 1.275 ± 0.321
1.346HisSer: 1.346 ± 0.286
1.204HisThr: 1.204 ± 0.279
1.346HisVal: 1.346 ± 0.344
0.637HisTrp: 0.637 ± 0.224
0.567HisTyr: 0.567 ± 0.201
0.0HisXaa: 0.0 ± 0.0
Ile
4.958IleAla: 4.958 ± 0.521
0.567IleCys: 0.567 ± 0.214
3.754IleAsp: 3.754 ± 0.514
3.117IleGlu: 3.117 ± 0.491
1.417IlePhe: 1.417 ± 0.33
3.258IleGly: 3.258 ± 0.498
1.629IleHis: 1.629 ± 0.4
2.904IleIle: 2.904 ± 0.559
3.4IleLys: 3.4 ± 0.445
3.329IleLeu: 3.329 ± 0.511
1.912IleMet: 1.912 ± 0.338
2.904IleAsn: 2.904 ± 0.596
2.337IlePro: 2.337 ± 0.431
2.408IleGln: 2.408 ± 0.357
3.046IleArg: 3.046 ± 0.378
3.046IleSer: 3.046 ± 0.611
4.108IleThr: 4.108 ± 0.583
4.037IleVal: 4.037 ± 0.691
0.637IleTrp: 0.637 ± 0.16
1.417IleTyr: 1.417 ± 0.286
0.0IleXaa: 0.0 ± 0.0
Lys
7.225LysAla: 7.225 ± 1.071
0.283LysCys: 0.283 ± 0.169
3.683LysAsp: 3.683 ± 0.533
3.187LysGlu: 3.187 ± 0.646
1.204LysPhe: 1.204 ± 0.302
4.25LysGly: 4.25 ± 0.557
1.417LysHis: 1.417 ± 0.346
2.267LysIle: 2.267 ± 0.344
3.329LysLys: 3.329 ± 0.565
3.825LysLeu: 3.825 ± 0.527
2.408LysMet: 2.408 ± 0.422
2.479LysAsn: 2.479 ± 0.412
2.975LysPro: 2.975 ± 0.502
2.833LysGln: 2.833 ± 0.644
4.179LysArg: 4.179 ± 0.686
2.337LysSer: 2.337 ± 0.422
3.542LysThr: 3.542 ± 0.573
2.55LysVal: 2.55 ± 0.487
1.133LysTrp: 1.133 ± 0.254
2.196LysTyr: 2.196 ± 0.435
0.0LysXaa: 0.0 ± 0.0
Leu
7.721LeuAla: 7.721 ± 0.62
0.921LeuCys: 0.921 ± 0.292
6.304LeuAsp: 6.304 ± 0.645
3.683LeuGlu: 3.683 ± 0.561
2.267LeuPhe: 2.267 ± 0.365
6.587LeuGly: 6.587 ± 0.758
1.7LeuHis: 1.7 ± 0.397
3.471LeuIle: 3.471 ± 0.561
4.037LeuLys: 4.037 ± 0.478
5.242LeuLeu: 5.242 ± 0.809
1.842LeuMet: 1.842 ± 0.316
3.825LeuAsn: 3.825 ± 0.694
2.904LeuPro: 2.904 ± 0.468
2.337LeuGln: 2.337 ± 0.471
5.029LeuArg: 5.029 ± 0.749
5.312LeuSer: 5.312 ± 0.615
5.1LeuThr: 5.1 ± 0.502
4.887LeuVal: 4.887 ± 0.647
1.204LeuTrp: 1.204 ± 0.252
2.337LeuTyr: 2.337 ± 0.486
0.0LeuXaa: 0.0 ± 0.0
Met
2.479MetAla: 2.479 ± 0.451
0.283MetCys: 0.283 ± 0.148
2.054MetAsp: 2.054 ± 0.337
1.275MetGlu: 1.275 ± 0.308
0.779MetPhe: 0.779 ± 0.199
2.267MetGly: 2.267 ± 0.433
0.85MetHis: 0.85 ± 0.237
1.983MetIle: 1.983 ± 0.391
1.346MetLys: 1.346 ± 0.28
2.621MetLeu: 2.621 ± 0.39
0.921MetMet: 0.921 ± 0.255
1.062MetAsn: 1.062 ± 0.247
1.275MetPro: 1.275 ± 0.313
1.842MetGln: 1.842 ± 0.319
2.762MetArg: 2.762 ± 0.547
2.267MetSer: 2.267 ± 0.434
1.842MetThr: 1.842 ± 0.362
1.983MetVal: 1.983 ± 0.296
0.354MetTrp: 0.354 ± 0.154
1.062MetTyr: 1.062 ± 0.268
0.0MetXaa: 0.0 ± 0.0
Asn
4.817AsnAla: 4.817 ± 0.643
0.212AsnCys: 0.212 ± 0.142
2.125AsnAsp: 2.125 ± 0.403
2.479AsnGlu: 2.479 ± 0.42
1.346AsnPhe: 1.346 ± 0.316
3.896AsnGly: 3.896 ± 0.505
0.708AsnHis: 0.708 ± 0.253
2.408AsnIle: 2.408 ± 0.361
2.833AsnLys: 2.833 ± 0.39
2.975AsnLeu: 2.975 ± 0.44
1.842AsnMet: 1.842 ± 0.321
1.558AsnAsn: 1.558 ± 0.423
2.621AsnPro: 2.621 ± 0.456
2.125AsnGln: 2.125 ± 0.472
1.912AsnArg: 1.912 ± 0.391
3.187AsnSer: 3.187 ± 0.525
2.479AsnThr: 2.479 ± 0.36
2.408AsnVal: 2.408 ± 0.41
0.496AsnTrp: 0.496 ± 0.26
1.487AsnTyr: 1.487 ± 0.286
0.0AsnXaa: 0.0 ± 0.0
Pro
3.046ProAla: 3.046 ± 0.514
0.142ProCys: 0.142 ± 0.101
3.117ProAsp: 3.117 ± 0.445
3.258ProGlu: 3.258 ± 0.441
1.7ProPhe: 1.7 ± 0.427
2.762ProGly: 2.762 ± 0.525
0.85ProHis: 0.85 ± 0.249
2.479ProIle: 2.479 ± 0.357
2.621ProLys: 2.621 ± 0.446
3.117ProLeu: 3.117 ± 0.476
0.921ProMet: 0.921 ± 0.234
1.487ProAsn: 1.487 ± 0.322
0.567ProPro: 0.567 ± 0.193
1.275ProGln: 1.275 ± 0.349
1.842ProArg: 1.842 ± 0.422
2.479ProSer: 2.479 ± 0.395
2.55ProThr: 2.55 ± 0.375
3.4ProVal: 3.4 ± 0.461
0.567ProTrp: 0.567 ± 0.195
0.992ProTyr: 0.992 ± 0.337
0.0ProXaa: 0.0 ± 0.0
Gln
4.462GlnAla: 4.462 ± 0.569
0.283GlnCys: 0.283 ± 0.13
2.479GlnAsp: 2.479 ± 0.401
2.904GlnGlu: 2.904 ± 0.473
1.558GlnPhe: 1.558 ± 0.354
3.4GlnGly: 3.4 ± 0.72
1.062GlnHis: 1.062 ± 0.314
2.692GlnIle: 2.692 ± 0.508
2.55GlnLys: 2.55 ± 0.453
3.187GlnLeu: 3.187 ± 0.441
1.204GlnMet: 1.204 ± 0.268
2.55GlnAsn: 2.55 ± 0.444
1.062GlnPro: 1.062 ± 0.265
3.471GlnGln: 3.471 ± 0.929
2.267GlnArg: 2.267 ± 0.446
2.692GlnSer: 2.692 ± 0.402
1.771GlnThr: 1.771 ± 0.287
3.258GlnVal: 3.258 ± 0.449
0.85GlnTrp: 0.85 ± 0.202
1.842GlnTyr: 1.842 ± 0.348
0.0GlnXaa: 0.0 ± 0.0
Arg
5.312ArgAla: 5.312 ± 0.593
0.567ArgCys: 0.567 ± 0.251
2.833ArgAsp: 2.833 ± 0.473
3.967ArgGlu: 3.967 ± 0.583
2.692ArgPhe: 2.692 ± 0.455
3.329ArgGly: 3.329 ± 0.499
1.346ArgHis: 1.346 ± 0.34
3.329ArgIle: 3.329 ± 0.456
3.754ArgLys: 3.754 ± 0.53
5.029ArgLeu: 5.029 ± 0.552
1.771ArgMet: 1.771 ± 0.401
2.975ArgAsn: 2.975 ± 0.51
1.912ArgPro: 1.912 ± 0.395
3.046ArgGln: 3.046 ± 0.519
3.4ArgArg: 3.4 ± 0.548
3.329ArgSer: 3.329 ± 0.456
3.329ArgThr: 3.329 ± 0.52
4.321ArgVal: 4.321 ± 0.396
1.062ArgTrp: 1.062 ± 0.282
1.912ArgTyr: 1.912 ± 0.468
0.0ArgXaa: 0.0 ± 0.0
Ser
6.021SerAla: 6.021 ± 0.667
0.496SerCys: 0.496 ± 0.204
3.187SerAsp: 3.187 ± 0.464
1.983SerGlu: 1.983 ± 0.443
2.125SerPhe: 2.125 ± 0.425
5.242SerGly: 5.242 ± 0.657
0.992SerHis: 0.992 ± 0.28
3.612SerIle: 3.612 ± 0.545
3.4SerLys: 3.4 ± 0.53
5.029SerLeu: 5.029 ± 0.738
2.054SerMet: 2.054 ± 0.453
2.054SerAsn: 2.054 ± 0.351
2.762SerPro: 2.762 ± 0.442
2.337SerGln: 2.337 ± 0.458
3.683SerArg: 3.683 ± 0.499
3.187SerSer: 3.187 ± 0.547
4.037SerThr: 4.037 ± 0.658
3.329SerVal: 3.329 ± 0.502
0.779SerTrp: 0.779 ± 0.272
2.125SerTyr: 2.125 ± 0.385
0.0SerXaa: 0.0 ± 0.0
Thr
5.667ThrAla: 5.667 ± 0.73
0.425ThrCys: 0.425 ± 0.165
3.187ThrAsp: 3.187 ± 0.406
3.329ThrGlu: 3.329 ± 0.509
1.771ThrPhe: 1.771 ± 0.346
5.242ThrGly: 5.242 ± 0.62
1.346ThrHis: 1.346 ± 0.337
3.4ThrIle: 3.4 ± 0.343
2.975ThrLys: 2.975 ± 0.412
4.817ThrLeu: 4.817 ± 0.579
1.204ThrMet: 1.204 ± 0.357
2.55ThrAsn: 2.55 ± 0.374
2.762ThrPro: 2.762 ± 0.412
2.408ThrGln: 2.408 ± 0.406
2.762ThrArg: 2.762 ± 0.419
4.321ThrSer: 4.321 ± 0.674
3.187ThrThr: 3.187 ± 0.552
3.612ThrVal: 3.612 ± 0.719
0.921ThrTrp: 0.921 ± 0.244
2.479ThrTyr: 2.479 ± 0.502
0.0ThrXaa: 0.0 ± 0.0
Val
5.808ValAla: 5.808 ± 0.561
0.85ValCys: 0.85 ± 0.328
3.471ValAsp: 3.471 ± 0.366
4.108ValGlu: 4.108 ± 0.491
2.054ValPhe: 2.054 ± 0.333
4.887ValGly: 4.887 ± 0.693
1.558ValHis: 1.558 ± 0.379
3.117ValIle: 3.117 ± 0.527
3.046ValLys: 3.046 ± 0.511
4.321ValLeu: 4.321 ± 0.484
1.629ValMet: 1.629 ± 0.37
2.904ValAsn: 2.904 ± 0.307
3.117ValPro: 3.117 ± 0.406
3.258ValGln: 3.258 ± 0.544
4.392ValArg: 4.392 ± 0.573
4.321ValSer: 4.321 ± 0.663
3.825ValThr: 3.825 ± 0.532
4.604ValVal: 4.604 ± 0.648
1.346ValTrp: 1.346 ± 0.365
2.267ValTyr: 2.267 ± 0.461
0.0ValXaa: 0.0 ± 0.0
Trp
1.629TrpAla: 1.629 ± 0.295
0.354TrpCys: 0.354 ± 0.156
1.133TrpAsp: 1.133 ± 0.335
0.567TrpGlu: 0.567 ± 0.246
0.779TrpPhe: 0.779 ± 0.248
0.567TrpGly: 0.567 ± 0.218
0.354TrpHis: 0.354 ± 0.199
0.708TrpIle: 0.708 ± 0.289
1.062TrpLys: 1.062 ± 0.356
1.842TrpLeu: 1.842 ± 0.278
0.567TrpMet: 0.567 ± 0.227
1.062TrpAsn: 1.062 ± 0.248
0.567TrpPro: 0.567 ± 0.167
1.062TrpGln: 1.062 ± 0.237
0.992TrpArg: 0.992 ± 0.238
0.637TrpSer: 0.637 ± 0.224
0.85TrpThr: 0.85 ± 0.214
1.204TrpVal: 1.204 ± 0.285
0.142TrpTrp: 0.142 ± 0.096
0.708TrpTyr: 0.708 ± 0.217
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.904TyrAla: 2.904 ± 0.328
0.142TyrCys: 0.142 ± 0.103
2.267TyrAsp: 2.267 ± 0.523
1.629TyrGlu: 1.629 ± 0.283
0.779TyrPhe: 0.779 ± 0.223
2.904TyrGly: 2.904 ± 0.352
1.204TyrHis: 1.204 ± 0.334
2.125TyrIle: 2.125 ± 0.388
1.842TyrLys: 1.842 ± 0.356
1.983TyrLeu: 1.983 ± 0.371
1.275TyrMet: 1.275 ± 0.259
2.267TyrAsn: 2.267 ± 0.333
1.133TyrPro: 1.133 ± 0.335
2.267TyrGln: 2.267 ± 0.363
2.337TyrArg: 2.337 ± 0.449
2.196TyrSer: 2.196 ± 0.363
1.912TyrThr: 1.912 ± 0.44
2.833TyrVal: 2.833 ± 0.502
0.425TyrTrp: 0.425 ± 0.17
1.346TyrTyr: 1.346 ± 0.263
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 61 proteins (14119 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski