Amino acid dipepetide frequency for Rhizobium phage RHEph01

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.335AlaAla: 14.335 ± 1.662
0.152AlaCys: 0.152 ± 0.119
4.499AlaAsp: 4.499 ± 0.588
7.93AlaGlu: 7.93 ± 1.04
3.889AlaPhe: 3.889 ± 0.475
8.159AlaGly: 8.159 ± 0.764
2.287AlaHis: 2.287 ± 0.453
5.261AlaIle: 5.261 ± 0.717
6.1AlaLys: 6.1 ± 0.885
9.607AlaLeu: 9.607 ± 1.235
2.897AlaMet: 2.897 ± 0.531
3.507AlaAsn: 3.507 ± 0.579
3.05AlaPro: 3.05 ± 0.693
4.27AlaGln: 4.27 ± 0.681
7.167AlaArg: 7.167 ± 0.805
7.015AlaSer: 7.015 ± 0.948
5.642AlaThr: 5.642 ± 1.029
6.252AlaVal: 6.252 ± 0.665
1.22AlaTrp: 1.22 ± 0.29
2.974AlaTyr: 2.974 ± 0.549
0.0AlaXaa: 0.0 ± 0.0
Cys
0.305CysAla: 0.305 ± 0.143
0.0CysCys: 0.0 ± 0.0
0.305CysAsp: 0.305 ± 0.146
0.61CysGlu: 0.61 ± 0.193
0.229CysPhe: 0.229 ± 0.119
0.152CysGly: 0.152 ± 0.109
0.305CysHis: 0.305 ± 0.18
0.152CysIle: 0.152 ± 0.109
0.305CysLys: 0.305 ± 0.144
0.61CysLeu: 0.61 ± 0.206
0.229CysMet: 0.229 ± 0.144
0.305CysAsn: 0.305 ± 0.154
0.61CysPro: 0.61 ± 0.214
0.152CysGln: 0.152 ± 0.114
0.534CysArg: 0.534 ± 0.186
0.305CysSer: 0.305 ± 0.133
0.381CysThr: 0.381 ± 0.169
0.534CysVal: 0.534 ± 0.27
0.076CysTrp: 0.076 ± 0.064
0.152CysTyr: 0.152 ± 0.111
0.0CysXaa: 0.0 ± 0.0
Asp
6.1AspAla: 6.1 ± 0.727
0.305AspCys: 0.305 ± 0.16
3.66AspAsp: 3.66 ± 0.655
5.032AspGlu: 5.032 ± 0.674
2.516AspPhe: 2.516 ± 0.36
4.041AspGly: 4.041 ± 0.57
1.067AspHis: 1.067 ± 0.3
2.669AspIle: 2.669 ± 0.424
3.05AspLys: 3.05 ± 0.377
5.261AspLeu: 5.261 ± 0.545
1.525AspMet: 1.525 ± 0.345
1.601AspAsn: 1.601 ± 0.31
2.821AspPro: 2.821 ± 0.564
3.126AspGln: 3.126 ± 0.435
3.279AspArg: 3.279 ± 0.445
2.974AspSer: 2.974 ± 0.555
3.584AspThr: 3.584 ± 0.64
3.126AspVal: 3.126 ± 0.4
1.144AspTrp: 1.144 ± 0.257
2.135AspTyr: 2.135 ± 0.505
0.0AspXaa: 0.0 ± 0.0
Glu
8.54GluAla: 8.54 ± 0.768
0.457GluCys: 0.457 ± 0.19
3.355GluAsp: 3.355 ± 0.529
3.965GluGlu: 3.965 ± 0.783
2.592GluPhe: 2.592 ± 0.505
3.736GluGly: 3.736 ± 0.667
1.296GluHis: 1.296 ± 0.301
3.507GluIle: 3.507 ± 0.43
3.736GluLys: 3.736 ± 0.689
4.956GluLeu: 4.956 ± 0.509
1.754GluMet: 1.754 ± 0.359
2.059GluAsn: 2.059 ± 0.449
2.135GluPro: 2.135 ± 0.385
2.059GluGln: 2.059 ± 0.412
4.804GluArg: 4.804 ± 0.634
2.211GluSer: 2.211 ± 0.495
3.736GluThr: 3.736 ± 0.657
3.812GluVal: 3.812 ± 0.468
1.372GluTrp: 1.372 ± 0.315
1.449GluTyr: 1.449 ± 0.378
0.0GluXaa: 0.0 ± 0.0
Phe
3.431PheAla: 3.431 ± 0.444
0.457PheCys: 0.457 ± 0.176
3.279PheAsp: 3.279 ± 0.506
2.211PheGlu: 2.211 ± 0.364
1.754PhePhe: 1.754 ± 0.352
3.431PheGly: 3.431 ± 0.646
0.457PheHis: 0.457 ± 0.194
2.364PheIle: 2.364 ± 0.462
3.126PheLys: 3.126 ± 0.498
3.279PheLeu: 3.279 ± 0.477
0.686PheMet: 0.686 ± 0.188
1.296PheAsn: 1.296 ± 0.316
1.906PhePro: 1.906 ± 0.383
1.601PheGln: 1.601 ± 0.436
2.059PheArg: 2.059 ± 0.406
2.135PheSer: 2.135 ± 0.545
2.364PheThr: 2.364 ± 0.393
2.974PheVal: 2.974 ± 0.43
0.381PheTrp: 0.381 ± 0.181
0.839PheTyr: 0.839 ± 0.253
0.0PheXaa: 0.0 ± 0.0
Gly
6.405GlyAla: 6.405 ± 0.961
0.762GlyCys: 0.762 ± 0.265
4.956GlyAsp: 4.956 ± 0.573
4.575GlyGlu: 4.575 ± 0.482
2.974GlyPhe: 2.974 ± 0.505
4.727GlyGly: 4.727 ± 0.577
1.906GlyHis: 1.906 ± 0.414
3.279GlyIle: 3.279 ± 0.636
3.431GlyLys: 3.431 ± 0.626
5.795GlyLeu: 5.795 ± 0.793
1.906GlyMet: 1.906 ± 0.462
3.584GlyAsn: 3.584 ± 0.507
1.677GlyPro: 1.677 ± 0.374
3.355GlyGln: 3.355 ± 0.527
4.804GlyArg: 4.804 ± 0.557
5.719GlySer: 5.719 ± 0.765
4.575GlyThr: 4.575 ± 0.593
4.575GlyVal: 4.575 ± 0.785
1.372GlyTrp: 1.372 ± 0.314
2.974GlyTyr: 2.974 ± 0.454
0.0GlyXaa: 0.0 ± 0.0
His
1.067HisAla: 1.067 ± 0.292
0.305HisCys: 0.305 ± 0.149
0.915HisAsp: 0.915 ± 0.292
0.534HisGlu: 0.534 ± 0.212
0.762HisPhe: 0.762 ± 0.24
1.449HisGly: 1.449 ± 0.383
0.534HisHis: 0.534 ± 0.304
1.525HisIle: 1.525 ± 0.361
0.839HisLys: 0.839 ± 0.27
2.135HisLeu: 2.135 ± 0.435
0.534HisMet: 0.534 ± 0.215
0.534HisAsn: 0.534 ± 0.168
0.915HisPro: 0.915 ± 0.344
0.686HisGln: 0.686 ± 0.251
1.372HisArg: 1.372 ± 0.401
1.372HisSer: 1.372 ± 0.268
1.372HisThr: 1.372 ± 0.318
1.906HisVal: 1.906 ± 0.51
0.381HisTrp: 0.381 ± 0.161
0.839HisTyr: 0.839 ± 0.26
0.0HisXaa: 0.0 ± 0.0
Ile
5.719IleAla: 5.719 ± 0.715
0.305IleCys: 0.305 ± 0.171
4.346IleAsp: 4.346 ± 0.518
2.821IleGlu: 2.821 ± 0.604
1.601IlePhe: 1.601 ± 0.273
3.431IleGly: 3.431 ± 0.39
1.22IleHis: 1.22 ± 0.344
2.44IleIle: 2.44 ± 0.514
2.592IleLys: 2.592 ± 0.376
3.736IleLeu: 3.736 ± 0.569
0.762IleMet: 0.762 ± 0.227
2.897IleAsn: 2.897 ± 0.551
2.364IlePro: 2.364 ± 0.364
1.83IleGln: 1.83 ± 0.3
3.812IleArg: 3.812 ± 0.552
3.05IleSer: 3.05 ± 0.488
3.431IleThr: 3.431 ± 0.531
3.202IleVal: 3.202 ± 0.495
1.067IleTrp: 1.067 ± 0.262
1.144IleTyr: 1.144 ± 0.235
0.0IleXaa: 0.0 ± 0.0
Lys
6.557LysAla: 6.557 ± 0.747
0.229LysCys: 0.229 ± 0.122
3.431LysAsp: 3.431 ± 0.676
3.05LysGlu: 3.05 ± 0.487
2.059LysPhe: 2.059 ± 0.328
4.117LysGly: 4.117 ± 0.549
0.686LysHis: 0.686 ± 0.281
2.745LysIle: 2.745 ± 0.408
3.355LysLys: 3.355 ± 0.588
5.947LysLeu: 5.947 ± 0.681
1.067LysMet: 1.067 ± 0.294
1.754LysAsn: 1.754 ± 0.461
2.669LysPro: 2.669 ± 0.615
1.754LysGln: 1.754 ± 0.522
3.507LysArg: 3.507 ± 0.635
3.126LysSer: 3.126 ± 0.498
4.499LysThr: 4.499 ± 0.675
5.109LysVal: 5.109 ± 0.836
1.067LysTrp: 1.067 ± 0.21
1.449LysTyr: 1.449 ± 0.349
0.0LysXaa: 0.0 ± 0.0
Leu
8.692LeuAla: 8.692 ± 0.952
0.152LeuCys: 0.152 ± 0.105
5.871LeuAsp: 5.871 ± 0.658
4.956LeuGlu: 4.956 ± 0.722
3.202LeuPhe: 3.202 ± 0.458
5.566LeuGly: 5.566 ± 0.92
0.915LeuHis: 0.915 ± 0.292
3.736LeuIle: 3.736 ± 0.476
5.642LeuLys: 5.642 ± 0.75
6.176LeuLeu: 6.176 ± 0.677
2.44LeuMet: 2.44 ± 0.418
3.431LeuAsn: 3.431 ± 0.479
4.27LeuPro: 4.27 ± 0.749
3.584LeuGln: 3.584 ± 0.559
5.185LeuArg: 5.185 ± 0.575
4.88LeuSer: 4.88 ± 0.7
4.422LeuThr: 4.422 ± 0.605
5.337LeuVal: 5.337 ± 0.605
1.296LeuTrp: 1.296 ± 0.393
2.44LeuTyr: 2.44 ± 0.388
0.0LeuXaa: 0.0 ± 0.0
Met
3.736MetAla: 3.736 ± 0.57
0.229MetCys: 0.229 ± 0.143
1.296MetAsp: 1.296 ± 0.286
0.839MetGlu: 0.839 ± 0.28
1.22MetPhe: 1.22 ± 0.315
2.211MetGly: 2.211 ± 0.587
0.381MetHis: 0.381 ± 0.164
1.372MetIle: 1.372 ± 0.357
1.144MetLys: 1.144 ± 0.342
1.83MetLeu: 1.83 ± 0.337
0.61MetMet: 0.61 ± 0.256
1.067MetAsn: 1.067 ± 0.235
1.372MetPro: 1.372 ± 0.286
0.991MetGln: 0.991 ± 0.308
1.22MetArg: 1.22 ± 0.313
1.525MetSer: 1.525 ± 0.33
2.135MetThr: 2.135 ± 0.413
1.22MetVal: 1.22 ± 0.298
0.229MetTrp: 0.229 ± 0.14
0.076MetTyr: 0.076 ± 0.064
0.0MetXaa: 0.0 ± 0.0
Asn
3.05AsnAla: 3.05 ± 0.615
0.152AsnCys: 0.152 ± 0.128
2.364AsnAsp: 2.364 ± 0.551
2.592AsnGlu: 2.592 ± 0.461
1.754AsnPhe: 1.754 ± 0.31
3.431AsnGly: 3.431 ± 0.473
0.762AsnHis: 0.762 ± 0.255
1.525AsnIle: 1.525 ± 0.311
1.449AsnLys: 1.449 ± 0.302
3.66AsnLeu: 3.66 ± 0.464
0.915AsnMet: 0.915 ± 0.217
1.144AsnAsn: 1.144 ± 0.259
2.364AsnPro: 2.364 ± 0.537
1.677AsnGln: 1.677 ± 0.424
2.287AsnArg: 2.287 ± 0.479
2.669AsnSer: 2.669 ± 0.471
2.211AsnThr: 2.211 ± 0.349
2.287AsnVal: 2.287 ± 0.376
0.61AsnTrp: 0.61 ± 0.235
0.991AsnTyr: 0.991 ± 0.3
0.0AsnXaa: 0.0 ± 0.0
Pro
6.405ProAla: 6.405 ± 0.962
0.076ProCys: 0.076 ± 0.067
1.906ProAsp: 1.906 ± 0.395
3.202ProGlu: 3.202 ± 0.542
2.059ProPhe: 2.059 ± 0.4
3.126ProGly: 3.126 ± 0.526
0.915ProHis: 0.915 ± 0.244
2.287ProIle: 2.287 ± 0.461
2.974ProLys: 2.974 ± 0.584
2.592ProLeu: 2.592 ± 0.395
0.61ProMet: 0.61 ± 0.221
2.135ProAsn: 2.135 ± 0.369
1.754ProPro: 1.754 ± 0.429
1.601ProGln: 1.601 ± 0.349
2.364ProArg: 2.364 ± 0.491
3.202ProSer: 3.202 ± 0.56
2.821ProThr: 2.821 ± 0.451
2.745ProVal: 2.745 ± 0.621
0.534ProTrp: 0.534 ± 0.179
0.991ProTyr: 0.991 ± 0.334
0.0ProXaa: 0.0 ± 0.0
Gln
3.584GlnAla: 3.584 ± 0.55
0.305GlnCys: 0.305 ± 0.144
1.982GlnAsp: 1.982 ± 0.329
2.44GlnGlu: 2.44 ± 0.371
1.601GlnPhe: 1.601 ± 0.384
2.592GlnGly: 2.592 ± 0.345
0.839GlnHis: 0.839 ± 0.252
2.364GlnIle: 2.364 ± 0.454
2.287GlnLys: 2.287 ± 0.393
3.812GlnLeu: 3.812 ± 0.705
1.144GlnMet: 1.144 ± 0.252
1.601GlnAsn: 1.601 ± 0.432
1.83GlnPro: 1.83 ± 0.414
1.677GlnGln: 1.677 ± 0.586
2.364GlnArg: 2.364 ± 0.344
2.135GlnSer: 2.135 ± 0.351
2.669GlnThr: 2.669 ± 0.397
2.592GlnVal: 2.592 ± 0.531
0.457GlnTrp: 0.457 ± 0.171
0.915GlnTyr: 0.915 ± 0.302
0.0GlnXaa: 0.0 ± 0.0
Arg
5.185ArgAla: 5.185 ± 0.745
0.534ArgCys: 0.534 ± 0.253
4.117ArgAsp: 4.117 ± 0.526
3.584ArgGlu: 3.584 ± 0.521
2.592ArgPhe: 2.592 ± 0.526
3.202ArgGly: 3.202 ± 0.327
1.22ArgHis: 1.22 ± 0.263
3.126ArgIle: 3.126 ± 0.464
4.27ArgLys: 4.27 ± 0.581
5.337ArgLeu: 5.337 ± 0.688
1.83ArgMet: 1.83 ± 0.419
2.592ArgAsn: 2.592 ± 0.476
2.364ArgPro: 2.364 ± 0.559
2.516ArgGln: 2.516 ± 0.358
3.279ArgArg: 3.279 ± 0.412
4.346ArgSer: 4.346 ± 0.626
2.897ArgThr: 2.897 ± 0.436
4.499ArgVal: 4.499 ± 0.535
1.372ArgTrp: 1.372 ± 0.341
1.754ArgTyr: 1.754 ± 0.363
0.0ArgXaa: 0.0 ± 0.0
Ser
7.625SerAla: 7.625 ± 1.072
0.152SerCys: 0.152 ± 0.104
3.05SerAsp: 3.05 ± 0.54
2.592SerGlu: 2.592 ± 0.417
2.974SerPhe: 2.974 ± 0.476
6.939SerGly: 6.939 ± 0.684
0.991SerHis: 0.991 ± 0.252
3.66SerIle: 3.66 ± 0.616
3.126SerLys: 3.126 ± 0.369
5.109SerLeu: 5.109 ± 0.61
1.525SerMet: 1.525 ± 0.287
1.754SerAsn: 1.754 ± 0.378
2.364SerPro: 2.364 ± 0.412
2.135SerGln: 2.135 ± 0.454
3.66SerArg: 3.66 ± 0.694
4.88SerSer: 4.88 ± 1.162
3.05SerThr: 3.05 ± 0.634
4.88SerVal: 4.88 ± 0.778
0.686SerTrp: 0.686 ± 0.2
2.364SerTyr: 2.364 ± 0.724
0.0SerXaa: 0.0 ± 0.0
Thr
5.261ThrAla: 5.261 ± 0.645
0.229ThrCys: 0.229 ± 0.127
3.431ThrAsp: 3.431 ± 0.538
3.889ThrGlu: 3.889 ± 0.638
3.05ThrPhe: 3.05 ± 0.507
4.727ThrGly: 4.727 ± 0.684
1.677ThrHis: 1.677 ± 0.405
4.27ThrIle: 4.27 ± 0.711
4.804ThrLys: 4.804 ± 0.823
4.27ThrLeu: 4.27 ± 0.71
1.22ThrMet: 1.22 ± 0.302
2.44ThrAsn: 2.44 ± 0.659
3.66ThrPro: 3.66 ± 0.484
2.059ThrGln: 2.059 ± 0.295
2.745ThrArg: 2.745 ± 0.546
3.812ThrSer: 3.812 ± 0.597
3.66ThrThr: 3.66 ± 0.606
4.117ThrVal: 4.117 ± 0.67
0.61ThrTrp: 0.61 ± 0.226
1.677ThrTyr: 1.677 ± 0.342
0.0ThrXaa: 0.0 ± 0.0
Val
6.252ValAla: 6.252 ± 0.715
0.991ValCys: 0.991 ± 0.288
3.965ValAsp: 3.965 ± 0.606
4.727ValGlu: 4.727 ± 0.786
1.982ValPhe: 1.982 ± 0.414
5.185ValGly: 5.185 ± 0.693
1.296ValHis: 1.296 ± 0.36
3.431ValIle: 3.431 ± 0.648
4.117ValLys: 4.117 ± 0.673
4.346ValLeu: 4.346 ± 0.713
1.601ValMet: 1.601 ± 0.344
2.897ValAsn: 2.897 ± 0.471
4.27ValPro: 4.27 ± 0.451
2.211ValGln: 2.211 ± 0.325
3.507ValArg: 3.507 ± 0.693
4.346ValSer: 4.346 ± 0.549
5.414ValThr: 5.414 ± 0.719
4.575ValVal: 4.575 ± 0.669
0.762ValTrp: 0.762 ± 0.29
1.144ValTyr: 1.144 ± 0.287
0.0ValXaa: 0.0 ± 0.0
Trp
1.372TrpAla: 1.372 ± 0.354
0.305TrpCys: 0.305 ± 0.163
0.686TrpAsp: 0.686 ± 0.241
0.305TrpGlu: 0.305 ± 0.16
0.457TrpPhe: 0.457 ± 0.187
0.839TrpGly: 0.839 ± 0.227
0.457TrpHis: 0.457 ± 0.235
0.686TrpIle: 0.686 ± 0.244
0.762TrpLys: 0.762 ± 0.228
1.372TrpLeu: 1.372 ± 0.388
0.61TrpMet: 0.61 ± 0.193
0.61TrpAsn: 0.61 ± 0.234
0.457TrpPro: 0.457 ± 0.208
0.686TrpGln: 0.686 ± 0.212
0.839TrpArg: 0.839 ± 0.262
1.449TrpSer: 1.449 ± 0.356
1.372TrpThr: 1.372 ± 0.249
1.449TrpVal: 1.449 ± 0.303
0.152TrpTrp: 0.152 ± 0.097
0.381TrpTyr: 0.381 ± 0.15
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.669TyrAla: 2.669 ± 0.44
0.152TyrCys: 0.152 ± 0.096
1.601TyrAsp: 1.601 ± 0.312
1.677TyrGlu: 1.677 ± 0.324
0.762TyrPhe: 0.762 ± 0.2
2.287TyrGly: 2.287 ± 0.404
0.762TyrHis: 0.762 ± 0.262
1.372TyrIle: 1.372 ± 0.271
1.067TyrLys: 1.067 ± 0.286
2.364TyrLeu: 2.364 ± 0.364
0.762TyrMet: 0.762 ± 0.256
0.686TyrAsn: 0.686 ± 0.251
1.449TyrPro: 1.449 ± 0.434
1.22TyrGln: 1.22 ± 0.247
1.83TyrArg: 1.83 ± 0.366
2.364TyrSer: 2.364 ± 0.694
1.372TyrThr: 1.372 ± 0.355
1.83TyrVal: 1.83 ± 0.551
0.457TyrTrp: 0.457 ± 0.199
0.686TyrTyr: 0.686 ± 0.249
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 55 proteins (13116 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski