Lachnospiraceae bacterium A2

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; unclassified Lachnospiraceae

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5159 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R9KL91|R9KL91_9FIRM Uncharacterized protein OS=Lachnospiraceae bacterium A2 OX=397290 GN=C810_01506 PE=4 SV=1
MM1 pKa = 7.64SDD3 pKa = 3.11KK4 pKa = 11.25NYY6 pKa = 10.86GLEE9 pKa = 3.99LYY11 pKa = 10.14KK12 pKa = 10.9LIMEE16 pKa = 4.71GDD18 pKa = 3.75KK19 pKa = 11.5NGFPYY24 pKa = 10.44VDD26 pKa = 3.27EE27 pKa = 5.33FGWISAGEE35 pKa = 3.94FCVWVSYY42 pKa = 10.81LWLNDD47 pKa = 3.32FMTDD51 pKa = 3.39LKK53 pKa = 11.38NIFGNGMFDD62 pKa = 5.14DD63 pKa = 5.11GGFDD67 pKa = 5.08ANMQEE72 pKa = 5.0DD73 pKa = 5.41GVCIDD78 pKa = 4.04LSEE81 pKa = 4.57TIGGYY86 pKa = 10.55LDD88 pKa = 3.48IEE90 pKa = 4.43EE91 pKa = 4.83VFPKK95 pKa = 10.46DD96 pKa = 3.66KK97 pKa = 10.74YY98 pKa = 9.73QHH100 pKa = 5.96

Molecular weight: 11.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R9KGK8|R9KGK8_9FIRM DUF3656 domain-containing protein OS=Lachnospiraceae bacterium A2 OX=397290 GN=C810_02484 PE=4 SV=1
MM1 pKa = 7.51EE2 pKa = 5.43RR3 pKa = 11.84NCGNRR8 pKa = 11.84TEE10 pKa = 4.43RR11 pKa = 11.84GRR13 pKa = 11.84EE14 pKa = 3.62VSRR17 pKa = 11.84RR18 pKa = 11.84NISRR22 pKa = 11.84NNGADD27 pKa = 3.31TQPQQRR33 pKa = 11.84EE34 pKa = 4.07WRR36 pKa = 11.84GSRR39 pKa = 11.84PGSIKK44 pKa = 10.09VSRR47 pKa = 11.84SGAILAALGLLFMGYY62 pKa = 9.32GASRR66 pKa = 11.84GEE68 pKa = 4.0MPVVLEE74 pKa = 4.03KK75 pKa = 10.79AINICMEE82 pKa = 4.78CIGIGG87 pKa = 3.31

Molecular weight: 9.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5159

0

5159

1470053

22

3031

284.9

31.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.731 ± 0.042

1.531 ± 0.015

5.15 ± 0.027

7.985 ± 0.042

4.141 ± 0.027

7.457 ± 0.035

1.767 ± 0.016

6.78 ± 0.033

7.071 ± 0.032

8.995 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.072 ± 0.018

4.192 ± 0.023

3.346 ± 0.024

3.789 ± 0.026

4.664 ± 0.031

5.829 ± 0.027

5.013 ± 0.034

6.403 ± 0.029

0.972 ± 0.013

4.113 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski